UniProtKB - P0C6F8 (R1A_BCHK3)
Protein
Replicase polyprotein 1a
Gene
1a
Organism
Bat coronavirus HKU3 (BtCoV) (SARS-like coronavirus HKU3)
Status
Functioni
The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 (By similarity).By similarity
Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity).By similarity
Miscellaneous
Bat coronavirus HKU3 is highly similar to SARS-CoV (SARS-like).
Catalytic activityi
- TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. EC:3.4.22.69
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1645 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1806 | For PL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3275 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 | |
Active sitei | 3379 | For 3CL-PRO activityPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1723 – 1760 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 4298 – 4314 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4341 – 4354 | By similarityAdd BLAST | 14 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: InterPro
- single-stranded RNA binding Source: InterPro
- thiol-dependent ubiquitin-specific protease activity Source: UniProtKB-EC
- transferase activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- induction by virus of catabolism of host mRNA Source: UniProtKB-KW
- induction by virus of host autophagy Source: UniProtKB-KW
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- suppression by virus of host IRF3 activity Source: UniProtKB-KW
- suppression by virus of host ISG15 activity Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
- viral genome replication Source: InterPro
- viral protein processing Source: InterPro
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1aShort name: pp1a Alternative name(s): ORF1a polyprotein Cleaved into the following 11 chains: Alternative name(s): Leader protein Alternative name(s): p65 homolog Alternative name(s): PL2-PRO Papain-like proteinase Short name: PL-PRO Alternative name(s): nsp5 Alternative name(s): Growth factor-like peptide Short name: GFL |
Gene namesi | ORF Names:1a |
Organismi | Bat coronavirus HKU3 (BtCoV) (SARS-like coronavirus HKU3) |
Taxonomic identifieri | 442736 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Betacoronavirus › Sarbecovirus › |
Virus hosti | Rhinolophus sinicus (Chinese rufous horseshoe bat) [TaxID: 89399] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
- host perinuclear region By similarity Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 2197 – 2217 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2298 – 2318 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2345 – 2365 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2744 – 2764 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 2986 – 3006 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3016 – 3036 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3048 – 3068 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3071 – 3091 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3099 – 3119 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3136 – 3156 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3558 – 3578 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3580 – 3600 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3606 – 3626 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3652 – 3672 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3679 – 3698 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 3722 – 3742 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 3750 – 3770 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- host cell membrane Source: UniProtKB-SubCell
- host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB-KW
Keywords - Cellular componenti
Host cytoplasm, Host membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000338074 | 1 – 4376 | Replicase polyprotein 1aAdd BLAST | 4376 | |
ChainiPRO_0000338075 | 1 – 179 | Non-structural protein 1By similarityAdd BLAST | 179 | |
ChainiPRO_0000338076 | 180 – 818 | Non-structural protein 2By similarityAdd BLAST | 639 | |
ChainiPRO_0000338077 | 819 – 2734 | Non-structural protein 3By similarityAdd BLAST | 1916 | |
ChainiPRO_0000338078 | 2735 – 3234 | Non-structural protein 4By similarityAdd BLAST | 500 | |
ChainiPRO_0000338079 | 3235 – 3540 | 3C-like proteinaseBy similarityAdd BLAST | 306 | |
ChainiPRO_0000338080 | 3541 – 3830 | Non-structural protein 6By similarityAdd BLAST | 290 | |
ChainiPRO_0000338081 | 3831 – 3913 | Non-structural protein 7By similarityAdd BLAST | 83 | |
ChainiPRO_0000338082 | 3914 – 4111 | Non-structural protein 8By similarityAdd BLAST | 198 | |
ChainiPRO_0000338083 | 4112 – 4224 | Non-structural protein 9By similarityAdd BLAST | 113 | |
ChainiPRO_0000338084 | 4225 – 4363 | Non-structural protein 10By similarityAdd BLAST | 139 | |
ChainiPRO_0000338085 | 4364 – 4376 | Non-structural protein 11Sequence analysisAdd BLAST | 13 |
Post-translational modificationi
Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 179 – 180 | Cleavage; by PL-PROBy similarity | 2 | |
Sitei | 818 – 819 | Cleavage; by PL-PROBy similarity | 2 | |
Sitei | 3234 – 3235 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3540 – 3541 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3830 – 3831 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 3913 – 3914 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 4111 – 4112 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 4224 – 4225 | Cleavage; by 3CL-PROBy similarity | 2 | |
Sitei | 4363 – 4364 | Cleavage; by 3CL-PROBy similarity | 2 |
Interactioni
Subunit structurei
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 998 – 1164 | MacroPROSITE-ProRule annotationAdd BLAST | 167 | |
Domaini | 1605 – 1869 | Peptidase C16PROSITE-ProRule annotationAdd BLAST | 265 | |
Domaini | 3235 – 3540 | Peptidase C30PROSITE-ProRule annotationAdd BLAST | 306 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2086 – 2365 | HD1By similarityAdd BLAST | 280 | |
Regioni | 2749 – 3156 | HD2By similarityAdd BLAST | 408 | |
Regioni | 3558 – 3770 | HD3By similarityAdd BLAST | 213 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 930 – 996 | Glu-richAdd BLAST | 67 | |
Compositional biasi | 2204 – 2207 | Poly-Leu | 4 | |
Compositional biasi | 3760 – 3763 | Poly-Cys | 4 |
Domaini
The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity
Sequence similaritiesi
Belongs to the coronaviruses polyprotein 1ab family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1723 – 1760 | C4-typePROSITE-ProRule annotationAdd BLAST | 38 | |
Zinc fingeri | 4298 – 4314 | By similarityAdd BLAST | 17 | |
Zinc fingeri | 4341 – 4354 | By similarityAdd BLAST | 14 |
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.30.30.590, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.40.220.10, 1 hit 3.40.220.20, 1 hit 3.40.220.30, 1 hit 3.40.50.11020, 1 hit 3.90.70.90, 1 hit |
InterProi | View protein in InterPro IPR043613, CoV_NSP2_C IPR043615, CoV_NSP2_N IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR022733, DPUP_SUD_C_bCoV IPR002589, Macro_dom IPR043472, Macro_dom-like IPR042570, NAR_sf IPR036333, NSP10_sf_CoV IPR021590, NSP1_bCoV IPR038030, NSP1_sf_bCoV IPR024375, NSP3_bCoV IPR024358, NSP3_N_bCoV IPR032592, NSP3_NAR_bCoV IPR038166, NSP3_PL2pro_sf_CoV IPR038400, NSP3_SUD-M_sf_bCoV IPR043478, NSP3_SUD-N_bCoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF11501, bCoV_NSP1, 1 hit PF12379, bCoV_NSP3_N, 1 hit PF12124, bCoV_SUD_C, 1 hit PF11633, bCoV_SUD_M, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF160099, SSF160099, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform Replicase polyprotein 1a (identifier: P0C6F8-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MESLVLGVNE KTHVQLSLPV LQVRDVLVRG FGDSVEEALS EAREHLKNGT
60 70 80 90 100
CGLVELEKGV LPQLEQPYVF IKRSDALSTN HGHKVVELVA ELDGIQFGRS
110 120 130 140 150
GITLGVLVPH VGETPIAYRN VLLRKNGNKG AGGHSFGIDL KSYDLGDELG
160 170 180 190 200
TDPIEDYEQN WNTKHGSGAL RELTRELNGG VVTRYVDNNF CGPDGYPLEC
210 220 230 240 250
IKDFLARAGK SMCTLSEQLD YIESKRGVYC CREHEHEIVW FTERSEKSYE
260 270 280 290 300
HQTPFEIKSA KKFDTFKGEC PKFVFPLNSK VKVIQPRVEK KKTEGFMGRI
310 320 330 340 350
RSVYPVATPQ ECNDMHLSTL MKCNHCDEVS WQTCDFLKAT CEQCGTENLV
360 370 380 390 400
CEGPTTCGYL PTNAVVKMPC PACQDPEVGP EHSVADYHNH SNIETRLRKG
410 420 430 440 450
GRTKCFGGCV FSYVGCYNKR AYWVPRASAN IGANHTGITG ENVETLNEDL
460 470 480 490 500
LEILNRERVN INIVGDFRFN EEVAIILASF SASPSAFIET VKGLDYKSFK
510 520 530 540 550
VIVESCGNYK VTNGKPVTGA WNIGQQRSIL TPLCGFPSQA AGVIRSIFSR
560 570 580 590 600
TLDAANHSIL DLQRAAVTTL DGISEQSLRL VDAMVYTSDL LTNSVVVMAY
610 620 630 640 650
VTGGLVQQTM QWLSNMLGTA VDKLKPVFTW VEAKLSAGVE FLRDAWEILK
660 670 680 690 700
FLITGVFDVI KGQIQVATDN IKECVKIFLG VVNKALEMCL DQVTIAGTKL
710 720 730 740 750
RALNLGEVFI AQSRGLYRQC IRGKEQLQLL MPLKAPKEVT FLEGDAHDTV
760 770 780 790 800
LTSEEVVLKS GELEALETPI DSFTSGAVVG TPVCINGLML LELENKEQYC
810 820 830 840 850
ALSPGLLATN NVFRLKGGAP VKGVTFGEDT VLEVQGYKNV KITFELDVRV
860 870 880 890 900
DKVLNEKCSV YTVESGTEVT EFACVVAEAV VKTLQPVSDL LTPMGIDLDE
910 920 930 940 950
WSVATFYLFD DAGEEKLSSR MYCSFYPPDE EEDCEECEDE EETCEHEYGT
960 970 980 990 1000
EDDYKGLPLE FGASTETPHV EEEEEEEDWL DDAIEAEPEP EPLPEEPVNQ
1010 1020 1030 1040 1050
FVGYLKLTDN VAIKCIDIVK EAQSAKPTVI VNAANTHLKH GGGVAGALNK
1060 1070 1080 1090 1100
ATNGAMQNES DEYIRQNGPL TVGGSCLLSG HNLAEKCLHV VGPNLNAGED
1110 1120 1130 1140 1150
VQLLKRAYEN FNSQDVLLAP LLSAGIFGAK PLQSLKMCVE IVRTQVYLAV
1160 1170 1180 1190 1200
NDKSLYDQIV LDYLDSLKPK VESPNKEEEP KLEEPKAVQP VAEKPVDVKP
1210 1220 1230 1240 1250
KIKACIDEVT TTLEETKFLT NKLLLFADIN GKLYQDSQNM LRGEDMSFLE
1260 1270 1280 1290 1300
KDAPYIVGDV ITSGDITCVI IPAKKSGGTT EMLARALKEV PVAEYITTYP
1310 1320 1330 1340 1350
GQGCAGYTLE EAKTALKKCK SAFYVLPSET PNEKEEVLGT VSWNLREMLA
1360 1370 1380 1390 1400
HAEETRKLMP ICLDVRAIMA TIQRKYKGIK VQEGIVDYGV RFFFYTSKEP
1410 1420 1430 1440 1450
VASIITKLNS LNEPLVTMPI GYVTHGLNLE EAARCMRSLK APAVVSVSSP
1460 1470 1480 1490 1500
DAVTAYNGYL TSSSKTPEEY FVETTSLAGS YRDWSYSGQR TELGVEFLKR
1510 1520 1530 1540 1550
GDKIVYHTTG SPIEFHLDGE VLPLDKLKSL LSLREVKTIK VFTTVDNTNL
1560 1570 1580 1590 1600
HTHIVDMSMT YGQQFGPTYL DGADVTKIKP HVNHEGKTFF VLPSDDTLRS
1610 1620 1630 1640 1650
EAFEYYHTID ESFLGRYMSA LNHTKKWKFP QVGGLTSIKW ADNNCYLSSV
1660 1670 1680 1690 1700
LLALQQVEVK FNAPALQEAY YRARAGDAAN FCALILAYSN KTVGELGDVR
1710 1720 1730 1740 1750
ETMTHLLQHA NLESAKRVLN VVCKHCGQKT TTLKGVEAVM YMGTLSYDEL
1760 1770 1780 1790 1800
KTGVSIPCVC GRNATQYLVQ QESSFVMMSA PPAEYKLQQG AFLCANEYTG
1810 1820 1830 1840 1850
NYQCGHYTHI TAKETLYRVD GAHLTKMSEY KGPVTDVFYK ETSYTTAIKP
1860 1870 1880 1890 1900
VSYKLDGVTY TEIEPKLDGY YKKGNAYYTE QPIDLVPTQP MPNASFDNFK
1910 1920 1930 1940 1950
LTCSNTKFAD DLNQMTGFKK PASRELTVTF FPDLNGDVVA IDYRHYSTSF
1960 1970 1980 1990 2000
KKGAKLVHKP ILWHINQTTN KTTYKPNIWC LRCLWSTKPV DTSNSFEVLV
2010 2020 2030 2040 2050
VEDTQGMDNL ACESQTTTSE EVVENPTVQK EIIECDVKTT EVVGNVILKP
2060 2070 2080 2090 2100
SEEGVKVTQE LGHEDLMAAY VEETSITIKK PNELSLALGL KTLATHGAAA
2110 2120 2130 2140 2150
INSVPWSKIL AYVKPFLGQT AVITSNCIKK CVQRVFSNYM PYVITLLFQL
2160 2170 2180 2190 2200
CTFTKSTNSR IKASLPTTIA KNSVKSVAKL CLDVCINYVK SPKFSKLFTI
2210 2220 2230 2240 2250
VMWLLLLSIC LGSLTYVTAV LGVCLSSLGV PSYCDGVREL YINSSNVTTM
2260 2270 2280 2290 2300
DFCQGYFPCS VCLSGLDSLD SYPALETIQV TISSYKLDLT FLGLAAEWLL
2310 2320 2330 2340 2350
AYMLFTKFFY LLGLSAIMQA FFGYFASHFI SNSWLMWFII SIVQMAPVSA
2360 2370 2380 2390 2400
MVRMYIFFAS FYYVWKSYVH IMDGCTSSTC MMCYKRNRAT RVECTTIVNG
2410 2420 2430 2440 2450
VKRSFYVYAN GGRGFCKAHN WNCLNCDTFC AGSTFISDEV ARDLSLQFKR
2460 2470 2480 2490 2500
PINPTDQSAY VVDSVTVKNG ALHLYFDKAG QKTYERHPLS HFVNLDNLRA
2510 2520 2530 2540 2550
NNTKGSLPIN VIVFDGKSKC EESAAKSASV YYSQLMCQPI LLLDQALVSD
2560 2570 2580 2590 2600
VGDSTEVSVK MFDAYVDTFS ATFSVPMEKL KALVATAHSE LAKGVALDGV
2610 2620 2630 2640 2650
LSTFVSAARQ GVVDTDVDTK DVIECLKLSH HSDIEVTGDS CNNFMLTYNK
2660 2670 2680 2690 2700
VENMTPRDLG ACIDCNARHI NAQVAKSHNV SLVWNVKDYM SLSEQLRKQI
2710 2720 2730 2740 2750
RSAAKKNNIP FRLTCATTRQ VVNVITTKIS LKGGKVVSTW FKLLLKVTLL
2760 2770 2780 2790 2800
CVLAALFCYV IMPVHSLSVH DGYTNEIIGY KAIQDGVTRD IVSTDDCFAN
2810 2820 2830 2840 2850
KHAGFDSWFS QRGGSYRNDK NCPVVAAIIT REIGFIVPGL PGTVLRALNG
2860 2870 2880 2890 2900
DFLHFLPRVF SAVGNICYTP SKLIEYSDFA TSACVLAAEC TIFKDAMGKP
2910 2920 2930 2940 2950
VPYCYDTNLL EGSISYSELR PDTRYVLMDG SIIQFPNTYL EGSVRVVTTF
2960 2970 2980 2990 3000
DAEYCRHGTC ERSEVGVCLS TSGRWVLNNE HYRALPGVFC GVDAMNLIAN
3010 3020 3030 3040 3050
IFTPLVQPVG ALDVSASVVA GGIIAILVTC AAYYFMKFRR AFGEYNHVVA
3060 3070 3080 3090 3100
ANALLFLMSF TILCLAPAYS FLPGVYSIFY LYLTFYFTND VSFLAHLQWF
3110 3120 3130 3140 3150
AMFSPIVPFW ITAIYVFCIS LKHFHWFFSN YLKKRVMFNG VTFSTFEEAA
3160 3170 3180 3190 3200
LCTFLLNKEM YLRLRSETLL PLTQYNRYLA LYNKYKYFSG ALDTTSYREA
3210 3220 3230 3240 3250
ACCHLAKALN DFSNSGADVL YQPPQTSITS AVLQSGFRKM AFPSGKVEGC
3260 3270 3280 3290 3300
MVQVTCGTTT LNGLWLDDTV YCPRHVVCTA EDMLNPNYDD LLIRKSNHSF
3310 3320 3330 3340 3350
LVQAGNVQLR VIGHSMQNCL LRLKVDTSNP KTPKYKFVRI QPGQTFSVLA
3360 3370 3380 3390 3400
CYNGSPSGVY QCAMRPNHTI KGSFLNGSCG SVGFNIDYDC VSFCYMHHME
3410 3420 3430 3440 3450
LPTGVHAGTD LEGKFYGPFV DRQTAQAAGT DTTITLNVLA WLYAAVINGD
3460 3470 3480 3490 3500
RWFLNRFTTT LNDFNLVAMK YNYEPLTQDH VDILGPLSAQ TGIAVLDMCA
3510 3520 3530 3540 3550
ALKELLQNGM NGRTILGSTI LEDEFTPFDV VRQCSGVTFQ GKFKKIVKGT
3560 3570 3580 3590 3600
HHWMLLTFLT SLLILVQSTQ WSLFFFVYEN AFLPFALGIM AVAACAMLLV
3610 3620 3630 3640 3650
KHKHAFLCLF LLPSLATVAY FNMVYMPASW VMRIMTWLEL ADTSLSGYRL
3660 3670 3680 3690 3700
KDCVMYASAL VLLILMTART VYDDAARRVW TLMNVITLVY KVYYGNSLDQ
3710 3720 3730 3740 3750
AISMWALVIS VTSNYSGVVT TIMFLARAIV FVCVEYYPLL FITGNTLQCI
3760 3770 3780 3790 3800
MLVYCFLGYC CCCYFGLFCL LNRYFRLTLG VYDYLVSTQE FRYMNSQGLL
3810 3820 3830 3840 3850
PPKSSIDAFK LNIKLLGIGG KPCIKVATVQ SKMSDVKCTS VVLLSVLQQL
3860 3870 3880 3890 3900
RVESSSKLWA QCVQLHNDIL LAKDTTEAFE KMVSLLSVLL SMQGAVDINK
3910 3920 3930 3940 3950
LCEEMLDNRA TLQAIASEFS SLPSYAAYAT AQEAYEQAVS NGDSEVVLKK
3960 3970 3980 3990 4000
LKKSLNVAKS EFDHDAAMQR KLEKMADQAM TQMYKQARSE DKRAKVTSAM
4010 4020 4030 4040 4050
QTMLFTMLRK LDNDALNNII NNARDGCVPL NIIPLTTAAK LMVVVPDYGT
4060 4070 4080 4090 4100
YKNTCDGNTF TYASALWEIQ QVVDADSKIV QLSEINMDNS PNLAWPLIVT
4110 4120 4130 4140 4150
ALRANSAVKL QNNELSPVAL RQMSCAAGTT QTACTDDNAL AYYNNAKGGR
4160 4170 4180 4190 4200
FVLALLSDHQ DLKWARFPKS DGTGTIYTEL EPPCRFVTDT PKGPKVKYLY
4210 4220 4230 4240 4250
FIKGLNNLNR GMVLGSLAAT VRLQAGNATE VPANSTVLSF CAFAVDPAKA
4260 4270 4280 4290 4300
YKDYLASGGQ PITNCVKMLC THTGTGQAIT VTPEANMDQE SFGGASCCLY
4310 4320 4330 4340 4350
CRCHIDHPNP KGFCDLKGKY VQIPTTCAND PVGFTLRNTV CTVCGMWKGY
4360 4370
GCSCDQLREP MMQSADASTF LNGFAV
Note: Produced by conventional translation.
Isoform Replicase polyprotein 1ab (identifier: P0C6W2-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6W2.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ022305 Genomic RNA No translation available. |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ022305 Genomic RNA No translation available. |
3D structure databases
BMRBi | P0C6F8 |
SMRi | P0C6F8 |
ModBasei | Search... |
Family and domain databases
Gene3Di | 1.10.150.420, 1 hit 1.10.1840.10, 1 hit 1.10.8.1190, 1 hit 1.10.8.370, 1 hit 2.30.30.590, 1 hit 2.40.10.10, 2 hits 2.40.10.250, 1 hit 2.40.10.290, 1 hit 3.10.20.350, 1 hit 3.10.20.540, 1 hit 3.40.220.10, 1 hit 3.40.220.20, 1 hit 3.40.220.30, 1 hit 3.40.50.11020, 1 hit 3.90.70.90, 1 hit |
InterProi | View protein in InterPro IPR043613, CoV_NSP2_C IPR043615, CoV_NSP2_N IPR043611, CoV_NSP3_C IPR043612, CoV_NSP4_N IPR043610, CoV_NSP6 IPR022733, DPUP_SUD_C_bCoV IPR002589, Macro_dom IPR043472, Macro_dom-like IPR042570, NAR_sf IPR036333, NSP10_sf_CoV IPR021590, NSP1_bCoV IPR038030, NSP1_sf_bCoV IPR024375, NSP3_bCoV IPR024358, NSP3_N_bCoV IPR032592, NSP3_NAR_bCoV IPR038166, NSP3_PL2pro_sf_CoV IPR038400, NSP3_SUD-M_sf_bCoV IPR043478, NSP3_SUD-N_bCoV IPR038083, NSP3A-like IPR032505, NSP4_C_CoV IPR038123, NSP4_C_sf_CoV IPR014828, NSP7_CoV IPR037204, NSP7_sf_CoV IPR014829, NSP8_CoV-like IPR037230, NSP8_sf_CoV IPR014822, NSP9_CoV IPR036499, NSP9_sf_CoV IPR013016, Peptidase_C16_CoV IPR008740, Peptidase_C30_CoV IPR043477, Peptidase_C30_dom3_CoV IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR043177, PLpro_N_sf_CoV IPR043503, PLpro_palm_finger_dom_CoV IPR043178, PLpro_thumb_sf_CoV IPR018995, RNA_synth_NSP10_CoV |
Pfami | View protein in Pfam PF16251, bCoV_NAR, 1 hit PF11501, bCoV_NSP1, 1 hit PF12379, bCoV_NSP3_N, 1 hit PF12124, bCoV_SUD_C, 1 hit PF11633, bCoV_SUD_M, 1 hit PF09401, CoV_NSP10, 1 hit PF19212, CoV_NSP2_C, 1 hit PF19211, CoV_NSP2_N, 1 hit PF19218, CoV_NSP3_C, 1 hit PF16348, CoV_NSP4_C, 1 hit PF19217, CoV_NSP4_N, 1 hit PF19213, CoV_NSP6, 1 hit PF08716, CoV_NSP7, 1 hit PF08717, CoV_NSP8, 1 hit PF08710, CoV_NSP9, 1 hit PF08715, CoV_peptidase, 1 hit PF01661, Macro, 1 hit PF05409, Peptidase_C30, 1 hit |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit |
SUPFAMi | SSF101816, SSF101816, 1 hit SSF140367, SSF140367, 1 hit SSF143076, SSF143076, 1 hit SSF144246, SSF144246, 1 hit SSF159936, SSF159936, 1 hit SSF160099, SSF160099, 1 hit SSF50494, SSF50494, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51442, M_PRO, 1 hit PS51154, MACRO, 1 hit PS51124, PEPTIDASE_C16, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | R1A_BCHK3 | |
Accessioni | P0C6F8Primary (citable) accession number: P0C6F8 Secondary accession number(s): Q3LZX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 10, 2008 |
Last sequence update: | June 10, 2008 | |
Last modified: | December 2, 2020 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |