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Entry version 83 (11 Dec 2019)
Sequence version 1 (10 Jun 2008)
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Protein

Replicase polyprotein 1a

Gene

1a

Organism
Bat coronavirus 133/2005 (BtCoV) (BtCoV/133/2005)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.1 Publication
The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity).PROSITE-ProRule annotation
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.By similarity
Nsp9 is a ssRNA-binding protein.By similarity
binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. EC:3.4.22.69
  • Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei1641For PL-PRO activityPROSITE-ProRule annotation1
Active sitei1807For PL-PRO activityPROSITE-ProRule annotation1
Active sitei3339For 3CL-PRO activityPROSITE-ProRule annotation1
Active sitei3446For 3CL-PRO activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri1721 – 1758C4-typePROSITE-ProRule annotationAdd BLAST38
Zinc fingeri4362 – 4378By similarityAdd BLAST17
Zinc fingeri4404 – 4417By similarityAdd BLAST14

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Protease, RNA-binding, Thiol protease
Biological processActivation of host autophagy by virus, Decay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host ISG15 by virus, Inhibition of host RLR pathway by virus, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway, Viral immunoevasion
LigandMetal-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Replicase polyprotein 1a
Short name:
pp1a
Alternative name(s):
ORF1a polyprotein
Cleaved into the following 11 chains:
Non-structural protein 1
Short name:
nsp1
Alternative name(s):
Leader protein
Non-structural protein 2
Short name:
nsp2
Alternative name(s):
p65 homolog
Non-structural protein 3 (EC:3.4.19.12, EC:3.4.22.69)
Short name:
nsp3
Alternative name(s):
PL2-PRO
Papain-like proteinase
Short name:
PL-PRO
Non-structural protein 4
Short name:
nsp4
3C-like proteinase (EC:3.4.22.-)
Short name:
3CL-PRO
Short name:
3CLp
Alternative name(s):
nsp5
Non-structural protein 6
Short name:
nsp6
Non-structural protein 7
Short name:
nsp7
Non-structural protein 8
Short name:
nsp8
Non-structural protein 9
Short name:
nsp9
Non-structural protein 10
Short name:
nsp10
Alternative name(s):
Growth factor-like peptide
Short name:
GFL
Non-structural protein 11
Short name:
nsp11
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
ORF Names:1a
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBat coronavirus 133/2005 (BtCoV) (BtCoV/133/2005)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri389230 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaNidoviralesCornidovirineaeCoronaviridaeOrthocoronavirinaeBetacoronavirusMerbecovirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiTylonycteris pachypus (Lesser bamboo bat) [TaxID: 258959]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000007449 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

  • host perinuclear region By similarity
  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
  • host perinuclear region By similarity
  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
  • host perinuclear region By similarity
  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity
  • host perinuclear region By similarity
  • Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes (By similarity).By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei2152 – 2172HelicalSequence analysisAdd BLAST21
Transmembranei2229 – 2249HelicalSequence analysisAdd BLAST21
Transmembranei2333 – 2353HelicalSequence analysisAdd BLAST21
Transmembranei2357 – 2377HelicalSequence analysisAdd BLAST21
Transmembranei2382 – 2402HelicalSequence analysisAdd BLAST21
Transmembranei2807 – 2827HelicalSequence analysisAdd BLAST21
Transmembranei3079 – 3099HelicalSequence analysisAdd BLAST21
Transmembranei3112 – 3132HelicalSequence analysisAdd BLAST21
Transmembranei3156 – 3176HelicalSequence analysisAdd BLAST21
Transmembranei3610 – 3630HelicalSequence analysisAdd BLAST21
Transmembranei3644 – 3664HelicalSequence analysisAdd BLAST21
Transmembranei3669 – 3689HelicalSequence analysisAdd BLAST21
Transmembranei3714 – 3734HelicalSequence analysisAdd BLAST21
Transmembranei3742 – 3762HelicalSequence analysisAdd BLAST21
Transmembranei3791 – 3811HelicalSequence analysisAdd BLAST21
Transmembranei3815 – 3835HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003380621 – 4441Replicase polyprotein 1aAdd BLAST4441
ChainiPRO_00003380631 – 195Non-structural protein 1Sequence analysisAdd BLAST195
ChainiPRO_0000338064196 – 847Non-structural protein 2Sequence analysisAdd BLAST652
ChainiPRO_0000338065848 – 2791Non-structural protein 3Sequence analysisAdd BLAST1944
ChainiPRO_00003380662792 – 3298Non-structural protein 4Sequence analysisAdd BLAST507
ChainiPRO_00003380673299 – 36043C-like proteinaseSequence analysisAdd BLAST306
ChainiPRO_00003380683605 – 3896Non-structural protein 6Sequence analysisAdd BLAST292
ChainiPRO_00003380693897 – 3979Non-structural protein 7Sequence analysisAdd BLAST83
ChainiPRO_00003380703980 – 4178Non-structural protein 8Sequence analysisAdd BLAST199
ChainiPRO_00003380714179 – 4288Non-structural protein 9Sequence analysisAdd BLAST110
ChainiPRO_00003380734288 – 4441Non-structural protein 11Sequence analysisAdd BLAST154
ChainiPRO_00003380724289 – 4427Non-structural protein 10Sequence analysisAdd BLAST139

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei195 – 196CleavageSequence analysis2
Sitei847 – 848Cleavage; by PL-PROSequence analysis2
Sitei2791 – 2792Cleavage; by PL-PROSequence analysis2
Sitei3298 – 3299Cleavage; by 3CL-PROSequence analysis2
Sitei3604 – 3605Cleavage; by 3CL-PROSequence analysis2
Sitei3896 – 3897Cleavage; by 3CL-PROSequence analysis2
Sitei3979 – 3980Cleavage; by 3CL-PROSequence analysis2
Sitei4178 – 4179Cleavage; by 3CL-PROSequence analysis2
Sitei4288 – 4289Cleavage; by 3CL-PROSequence analysis2
Sitei4427 – 4428Cleavage; by 3CL-PROSequence analysis2

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P0C6F7

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer (By similarity).

By similarity

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P0C6F7

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1159 – 1328MacroPROSITE-ProRule annotationAdd BLAST170
Domaini1600 – 1871Peptidase C16PROSITE-ProRule annotationAdd BLAST272
Domaini3299 – 3604Peptidase C30PROSITE-ProRule annotationAdd BLAST306

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni2119 – 2402HD1By similarityAdd BLAST284
Regioni2807 – 3176HD2By similarityAdd BLAST370
Regioni3610 – 3835HD3By similarityAdd BLAST226

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi960 – 1059Glu-richAdd BLAST100

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.By similarity

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1721 – 1758C4-typePROSITE-ProRule annotationAdd BLAST38
Zinc fingeri4362 – 4378By similarityAdd BLAST17
Zinc fingeri4404 – 4417By similarityAdd BLAST14

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.150.420, 1 hit
1.10.8.370, 1 hit
2.40.10.250, 1 hit
2.40.10.290, 1 hit
3.10.20.350, 1 hit
3.40.220.20, 1 hit
3.40.50.11020, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR032505 Corona_NSP4_C
IPR002589 Macro_dom
IPR032592 NAR_dom
IPR042570 NAR_sf
IPR036333 NSP10_sf
IPR024375 Nsp3_coronavir
IPR038400 Nsp3_coronavir_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR037204 NSP7_sf
IPR014829 NSP8
IPR037230 NSP8_sf
IPR014822 NSP9
IPR036499 NSP9_sf
IPR008740 Peptidase_C30
IPR013016 Peptidase_C30/C16
IPR009003 Peptidase_S1_PA
IPR038083 R1a/1ab
IPR018995 RNA_synth_NSP10_coronavirus
IPR014827 Viral_protease

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF16348 Corona_NSP4_C, 1 hit
PF01661 Macro, 1 hit
PF16251 NAR, 1 hit
PF09401 NSP10, 1 hit
PF08716 nsp7, 1 hit
PF08717 nsp8, 1 hit
PF08710 nsp9, 1 hit
PF05409 Peptidase_C30, 1 hit
PF11633 SUD-M, 1 hit
PF08715 Viral_protease, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00506 A1pp, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF101816 SSF101816, 1 hit
SSF140367 SSF140367, 1 hit
SSF143076 SSF143076, 1 hit
SSF144246 SSF144246, 1 hit
SSF159936 SSF159936, 1 hit
SSF50494 SSF50494, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51442 M_PRO, 1 hit
PS51154 MACRO, 1 hit
PS51124 PEPTIDASE_C16, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by ribosomal frameshifting. AlignAdd to basket
Isoform Replicase polyprotein 1a (identifier: P0C6F7-1) [UniParc]FASTAAdd to basket
Also known as: pp1a, ORF1a polyprotein

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLSKAGVTTQ GARGKYRAEL YNEKRSDHVA CTVPLCDTED MASKLTPWFE
60 70 80 90 100
DGETAFNQVS SILKEKGKIL FVPMHMQRAM KFLPGPRVYL VERLTGGMLS
110 120 130 140 150
KHFLVNQLAY KDHVGAAMMR TTLNVKPLGM FFPYDSSLET GEHTFLLRKN
160 170 180 190 200
GLGGQLFRER PWDRKETPYV EILDDLEADP TGKYSQNLLK KLIGGDCIPV
210 220 230 240 250
DQYMCGKNGK PIADYAKIVA KEGLTTLADI EVDVKSRMDS DRFIVLNKKL
260 270 280 290 300
YRVVWNVTRR NVPYSKQTAF TVVSVIQCDD KESVPEHTFT IGSQILMVSP
310 320 330 340 350
LKATNNKNFN LKQRLLHTFY GKEAVQQPGY IYHSAYVDCN ACGRGTWCTG
360 370 380 390 400
NAIQGFACDC GANYSANDVD LQSSGLVPKN ALFLANCPCA NNGACSHNAA
410 420 430 440 450
QVYSILDGKA CVEVGGKSFT LTFGGVVYAY MGCCDGTMYF VPRAKSCVSR
460 470 480 490 500
IGDAIFTGCT GTWDKVVETA NLFLEKAQHS LNFCQQFALT EVVLAILSGT
510 520 530 540 550
TSTFEELRDL CHNASYEKVR DHLVNHGFVV TIGDYIRDAI NIGANGVCNA
560 570 580 590 600
TINAPFIAFT GLGESFKKVA AIPWKICSNL KSALDYYCSN IMFRVFPYDI
610 620 630 640 650
PCDVNDFVEL LLDCGKLTVA TSYFVLRYLD EKFDTVLGTV SNACQTALSS
660 670 680 690 700
FLNACVAASR ATAGFISDMF KLFKVLMHKL YVYTSCGYVA VAEHSSKIVQ
710 720 730 740 750
QVLDIMSKAM KLLHTNVSWA GTKLSAIIYE GREALLFNSG TYFCLSTKAK
760 770 780 790 800
TLQDQMNLVL PGDYNKKTLG ILDPVPNADT IDVTANSTVV DVVHGQLEPT
810 820 830 840 850
NEHGPSMIVG NYVLVSDKLF VRTDDEEFYP LCINGKVVST LFRLKGGMPS
860 870 880 890 900
KKVTFGDVNT VEVTAYRSVS ITYDIHPVLD ALLSSSKLAT FTVEKDLLVE
910 920 930 940 950
DFVDVIKDEV LTLLTPLLRG YDIDGFDVED FIDVPCYVYN QDGDCAWSSN
960 970 980 990 1000
MTFSINPVED VEEVEEFIED DYLSDELPIA DDEEAWTRAV EEVMPLDDIL
1010 1020 1030 1040 1050
VAEIELEEDL PLETALESVE AEVGESISDE LCVVETAKAQ EPSVESTDST
1060 1070 1080 1090 1100
PSTSTVVSEN DLSVKPMSRV AETGDVLEVE TAVVGGPVSD VTASVVTNDI
1110 1120 1130 1140 1150
VSVEQAQQCG VSSLPIQDEA SENQVHQVPD LQCTSETKVE IVQPRQDLRP
1160 1170 1180 1190 1200
RRLRKSKVDL SKYKHTVINN SVTLVLGDAI QIASLLPKCV LVNAANRHLK
1210 1220 1230 1240 1250
HGGGIAGAIN KASGGDVQEE SDEYISNSGP LHVGDSVLLK GYGLADAILR
1260 1270 1280 1290 1300
VVGPDARNNE DAALLKRCYK TFNKHTIVVT PLISSGIFSV DPKVSFEYLL
1310 1320 1330 1340 1350
ANVTTTTYVV VNNEDIYNTL ATPSKPDGLV YSFEGWRGTV RTAKNYGFTC
1360 1370 1380 1390 1400
FICTEYSANV KFLRTKGVDT TKKIQTVDGV SYYLYSARDA LTDVIAAANG
1410 1420 1430 1440 1450
CPGICAMPFG YVTHGLDLAQ SGNYVRQVKV PYVCLLASKE QIPIMNSDVA
1460 1470 1480 1490 1500
IQTPETAFIN NVTSNGGYHS WHLVSGDLIV KDVCYKKLLH WSGQTICYAD
1510 1520 1530 1540 1550
NKFYVVKNDV ALPFSDLEAC RAYLTSRAAQ QVNIEVLVTI DGVNFRTVIL
1560 1570 1580 1590 1600
NDATTFRKQL GATFYKGVDI SDALPTVKMG GESLFVADNL SESEEVVLKE
1610 1620 1630 1640 1650
YYGTSDVTFL QRYYSLQPLV QQWKFVVHDG VKSLKLSNYN CYINATIMMI
1660 1670 1680 1690 1700
DMLHDIKFVV PALQNAYLRY KGGDPYDFLA LIMAYGDCTF DNPDDEAKLL
1710 1720 1730 1740 1750
HTLLAKAELT VSAKMVWREW CTVCGIRDIE YTGMRACVYA GVNSMEELQS
1760 1770 1780 1790 1800
VFNETCVCGS VKHRQLVEHS TPWLLVSGLN EVKVSTSTDP VYRAFNVFQG
1810 1820 1830 1840 1850
VETSVGHYVH VRVKDGLFYK YDSGSLTKTS DMKCKMTSVW YPKVRYTADC
1860 1870 1880 1890 1900
NVVVYDLDGV TKVEVNPDLS NYYMKDGKYY TSKPTIKYSP ATILPGSVYS
1910 1920 1930 1940 1950
NSCLVGVDGT PGSDTISKFF NDLLGFDETK PISKKLTYSL LPNEDGDVLL
1960 1970 1980 1990 2000
SEFNNYNPVY KKGVMLKGKP ILWVNNGVCD SALNKPNRAS LRQLYDVAPI
2010 2020 2030 2040 2050
VLDNKYTVLQ DNTSQLIEPN VPVVEDVSIT TRKLIEVKCK GLNKPFVKGN
2060 2070 2080 2090 2100
FSFVNDPNGV TVVDTLGLTE LRALYVDINT RYIVLRDNNW SSLFKLHTVE
2110 2120 2130 2140 2150
SGDLQIVANG GSVTRRARVL LGASSLFASF AKITVTATTA ACKTAGRSFC
2160 2170 2180 2190 2200
KFVVNYGVLQ NMFLFLKMLF FLPFNYLWPK KQPTVDVGVS GLRTAGVVTT
2210 2220 2230 2240 2250
NIVKQCGTAA YYMLLGKFKR VDWKATLRLF LLLCTTILLL SSIYHLVIFN
2260 2270 2280 2290 2300
QVLSSDVMLE DATGILAMYK EVRSYLGIRT LCDGLAVEYR NTSFDVVDFC
2310 2320 2330 2340 2350
SNRSVLCQWC LIGQDSLTRY SALQMLQTHI TSYVLNIDWI WFALEFFLAY
2360 2370 2380 2390 2400
VLYTSSFNVL LLVVTAQYFF AYTSAFVNWR AYNYIVSGLF FLVTHIPLHG
2410 2420 2430 2440 2450
LVRVYNFLAC LWFLRKFYSH VINGCKDTAC LLCYKRNRLT RVEASTIVCG
2460 2470 2480 2490 2500
TKRTFYIAAN GGTSYCCKHN WNCVECDTAG VGNTFICTEV ANDLTTTLRR
2510 2520 2530 2540 2550
LIKPTDQSHY YVDSVVVKDA VVELHYNRDG SSCYERYPLC YFTNLEKLKF
2560 2570 2580 2590 2600
KEVCKTPTGI PEHNFLIYDT NDRGQENLAR SACVYYSQVL CKPMLLVDVN
2610 2620 2630 2640 2650
LVTTVGDSRE IAIKMLDSFI NSFISLFSVS RDKLEKLINT ARDCVRRGDD
2660 2670 2680 2690 2700
FQTVLKTFTD AARGHAGVES DVETTMVVDA LQYAHKNDIQ LTTECYNNYV
2710 2720 2730 2740 2750
PGYIKPDSIN TLDLGCLIDL KAASVNQTSM RNANGACVWN SGDYMKLSDS
2760 2770 2780 2790 2800
FKRQIRIACR KCNIPFRLTT SKLRAADNIL SVKFSATKIV GGAPSWLLRV
2810 2820 2830 2840 2850
RDLTVKGYCI LTLFVFTVAV LSWFCLPSYS IATVNFNDDR ILTYKVIENG
2860 2870 2880 2890 2900
IVRDIAPNDA CFANKYGHFS KWFNENHGGV YRNSVDCPIT IAVIAGVAGA
2910 2920 2930 2940 2950
RVANVPATLA WVGRQIVLFV SRVFANTNVC FTPTNEIPYD TFSDSGCVLS
2960 2970 2980 2990 3000
SECTLFRDAE GNLNPFCYDP TVLPGASSYA DMKPHVRYDM YDSDMYIKFP
3010 3020 3030 3040 3050
EVIFESTLRI TKTLATQYCR FGSCEESAAG VCISTNGSWA LYNQNYSTRP
3060 3070 3080 3090 3100
GIYCGDDYFD IVRRLAVSLF QPVTYFQLST SLAMGLVLCV FLTAAFYYIN
3110 3120 3130 3140 3150
KVKRALADYT QCAVVAVVAA LLNSLCLCFI VANPLLVAPY TAMYYYATFY
3160 3170 3180 3190 3200
LTGEPAFIMH ISWYVMFGTV VPIWMLASYT VGVMLRHLFW VLAYFSKKHV
3210 3220 3230 3240 3250
DVFTDGKLNC SFQDAASNIF VIGKDTYVAL RNAITQDSFV RYLSLFNKYK
3260 3270 3280 3290 3300
YYSGAMDTAS YREACAAHLC KALQTYSETG SDILYQPPNC SVTSSVLQSG
3310 3320 3330 3340 3350
LVKMSAPSGA VENCIVQVTC GSMTLNGLWL DNTVWCPRHI MCPADQLTDP
3360 3370 3380 3390 3400
NYDALLISKT NHSFIVQKHI GAQANLRVVA HSMVGVLLKL TVDVANPSTP
3410 3420 3430 3440 3450
AYTFSTVKPG ASFSVLACYN GKPTGVFTVN LRHNSTIKGS FLCGSCGSVG
3460 3470 3480 3490 3500
YTENGGVLNF VYMHQMELSN GTHTGSSFDG VMYGAFEDKQ THQLQLTDKY
3510 3520 3530 3540 3550
CTINVVAWLY AAVLNGCKWF VKPTRVGIVT YNEWALSNQF TEFVGTQSID
3560 3570 3580 3590 3600
MLAHRTGVSV EQMLAAIQSL HAGFQGKTIL GQSTLEDEFT PDDVNMQVMG
3610 3620 3630 3640 3650
VVMQSGVKRI SYGFMHWLMS TLVLAYVSVM QLTKFTMWTY LFETIPTQMT
3660 3670 3680 3690 3700
PLLFGFMACV MFTVKHKHTF LSLFLLPVAL CLTYANIVYE PQTLVSSTLI
3710 3720 3730 3740 3750
AVANWLTPTS VYMRTTHLDF GLYISLSFVL AIIVRRLYRP SMSNLALALC
3760 3770 3780 3790 3800
SGVMWFYTYV IGDHSSPITY LMFITTLTSD YTITVFATVN LAKFISGLVF
3810 3820 3830 3840 3850
LYAPHLGFIL PEVKLVLLIY LCLGYMCTMY FGVFSLLNLK LRVPLGVYDY
3860 3870 3880 3890 3900
SVSTQEFRFL TGNGLHAPRN SWEALILNFK LLGIGGTPCI KVATVQSKLT
3910 3920 3930 3940 3950
DLKCTSVVLL TVLQQLHLES NSKAWSYCVK LHNEILAAVD PTEAFERFVC
3960 3970 3980 3990 4000
LFATLMSFSA NVDLDALAND LFENSSVLQA TLTEFSHLAT YAELETAQSS
4010 4020 4030 4040 4050
YQKALNSGDA SPQVLKALQK AVNVAKNAYE KDKAVARKLE RMAEQAMTSM
4060 4070 4080 4090 4100
YKQARAEDKK AKIVSAMQTM LFGMIKKLDN DVLNGVIANA RNGCVPLSIV
4110 4120 4130 4140 4150
PLCASNKLRV VIPDISVWNK VVNWPSVSYA GSLWDVTVIN NVDNEVVKPT
4160 4170 4180 4190 4200
DVVETNESLT WPLVIECSRA SSSAVKLQNN EIHPKGLKTM VVTAGIDQVN
4210 4220 4230 4240 4250
CSSSAVAYYE PVQGHRMVMG LLSENAHLKW AKVEGKDGFI NIELQPPCKF
4260 4270 4280 4290 4300
LIAGPKGPEI RYLYFVKNLN NLHRGQLLGH IAATVRLQAG ANTEFASNST
4310 4320 4330 4340 4350
VLTLVAFAVD PAKAYLDYVG SGGTPLSNYV KMLAPKTGTG VAISVKPEAT
4360 4370 4380 4390 4400
ADQETYGGAS VCLYCRAHIE HPDVSGVCKY KTRFVQIPAH VRDPVGFLLK
4410 4420 4430 4440
NVPCNVCQYW VGYGCNCDAL RNNTVPQSKD TNFLNESGVL V
Note: Produced by conventional translation.
Length:4,441
Mass (Da):492,464
Last modified:June 10, 2008 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA6E0DB4CDDA40AC7
GO
Isoform Replicase polyprotein 1ab (identifier: P0C6W1-1) [UniParc]FASTAAdd to basket
Also known as: pp1ab
The sequence of this isoform can be found in the external entry P0C6W1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:7,126
Mass (Da):795,781
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
DQ648794 Genomic RNA No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ648794 Genomic RNA No translation available.

3D structure databases

SMRiP0C6F7
ModBaseiSearch...

Proteomic databases

PRIDEiP0C6F7

Family and domain databases

Gene3Di1.10.150.420, 1 hit
1.10.8.370, 1 hit
2.40.10.250, 1 hit
2.40.10.290, 1 hit
3.10.20.350, 1 hit
3.40.220.20, 1 hit
3.40.50.11020, 1 hit
InterProiView protein in InterPro
IPR032505 Corona_NSP4_C
IPR002589 Macro_dom
IPR032592 NAR_dom
IPR042570 NAR_sf
IPR036333 NSP10_sf
IPR024375 Nsp3_coronavir
IPR038400 Nsp3_coronavir_sf
IPR038123 NSP4_C_sf
IPR014828 NSP7
IPR037204 NSP7_sf
IPR014829 NSP8
IPR037230 NSP8_sf
IPR014822 NSP9
IPR036499 NSP9_sf
IPR008740 Peptidase_C30
IPR013016 Peptidase_C30/C16
IPR009003 Peptidase_S1_PA
IPR038083 R1a/1ab
IPR018995 RNA_synth_NSP10_coronavirus
IPR014827 Viral_protease
PfamiView protein in Pfam
PF16348 Corona_NSP4_C, 1 hit
PF01661 Macro, 1 hit
PF16251 NAR, 1 hit
PF09401 NSP10, 1 hit
PF08716 nsp7, 1 hit
PF08717 nsp8, 1 hit
PF08710 nsp9, 1 hit
PF05409 Peptidase_C30, 1 hit
PF11633 SUD-M, 1 hit
PF08715 Viral_protease, 1 hit
SMARTiView protein in SMART
SM00506 A1pp, 1 hit
SUPFAMiSSF101816 SSF101816, 1 hit
SSF140367 SSF140367, 1 hit
SSF143076 SSF143076, 1 hit
SSF144246 SSF144246, 1 hit
SSF159936 SSF159936, 1 hit
SSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS51442 M_PRO, 1 hit
PS51154 MACRO, 1 hit
PS51124 PEPTIDASE_C16, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiR1A_BC133
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0C6F7
Secondary accession number(s): Q0Q4F3
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: December 11, 2019
This is version 83 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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