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Protein

60S ribosomal protein L27-A

Gene

RPL27A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 7380 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eL27 in yeast.Curated

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-31073-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L27-A1 Publication
Alternative name(s):
Large ribosomal subunit protein eL27-A1 Publication
Gene namesi
Name:RPL27A1 Publication
Synonyms:RPL27
Ordered Locus Names:YHR010W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR010W
SGDiS000001052 RPL27A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001260922 – 13660S ribosomal protein L27-AAdd BLAST135

Proteomic databases

MaxQBiP0C2H6
PaxDbiP0C2H6
PRIDEiP0C2H6
TopDownProteomicsiP0C2H6

PTM databases

iPTMnetiP0C2H6

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi36437, 95 interactors
IntActiP0C2H6, 4 interactors
MINTiP0C2H6
STRINGi4932.YHR010W

Structurei

Secondary structure

1136
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Beta strandi9 – 13Combined sources5
Beta strandi15 – 18Combined sources4
Beta strandi22 – 29Combined sources8
Beta strandi34 – 36Combined sources3
Beta strandi40 – 48Combined sources9
Beta strandi55 – 57Combined sources3
Helixi61 – 65Combined sources5
Beta strandi69 – 76Combined sources8
Helixi77 – 79Combined sources3
Beta strandi80 – 86Combined sources7
Helixi90 – 92Combined sources3
Turni93 – 95Combined sources3
Turni98 – 100Combined sources3
Beta strandi101 – 103Combined sources3
Helixi104 – 122Combined sources19
Turni123 – 127Combined sources5
Helixi128 – 131Combined sources4

3D structure databases

ProteinModelPortaliP0C2H6
SMRiP0C2H6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000010721
HOGENOMiHOG000210138
InParanoidiP0C2H6
KOiK02901
OMAiRYPRKVH
OrthoDBiEOG092C5ISN

Family and domain databases

Gene3Di2.30.30.770, 1 hit
InterProiView protein in InterPro
IPR038655 L27e_sf
IPR001141 Ribosomal_L27e
IPR018262 Ribosomal_L27e_CS
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR10497 PTHR10497, 1 hit
PfamiView protein in Pfam
PF01777 Ribosomal_L27e, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009396 Ribosomal_L27e, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
PROSITEiView protein in PROSITE
PS01107 RIBOSOMAL_L27E, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C2H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFLKAGKV AVVVRGRYAG KKVVIVKPHD EGSKSHPFGH ALVAGIERYP
60 70 80 90 100
LKVTKKHGAK KVAKRTKIKP FIKVVNYNHL LPTRYTLDVE AFKSVVSTET
110 120 130
FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF
Length:136
Mass (Da):15,531
Last modified:March 6, 2007 - v1
Checksum:iF3924B039AA2D85D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10400 Genomic DNA Translation: AAB68944.1
BK006934 Genomic DNA Translation: DAA06699.1
PIRiS46790
RefSeqiNP_011874.1, NM_001179140.1

Genome annotation databases

EnsemblFungiiYHR010W; YHR010W; YHR010W
GeneIDi856401
KEGGisce:YHR010W

Similar proteinsi

Entry informationi

Entry nameiRL27A_YEAST
AccessioniPrimary (citable) accession number: P0C2H6
Secondary accession number(s): D3DKV5, P38706
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: July 18, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

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