UniProtKB - P0C250 (COW1_CONBL)
Protein
Contryphan-Lo
Gene
N/A
Organism
Conus buxeus loroisii (Cone snail) (Conus loroisii)
Status
Functioni
Activates high voltage-activated calcium channels (Cav). This activity is in contrast to other contryphans that inhibit high voltage-gated calcium channels.1 Publication
Miscellaneous
Exists in two forms, due to cis-trans isomerization at 56-Cys-Pro-57.1 Publication
GO - Molecular functioni
- ion channel inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin |
Names & Taxonomyi
Protein namesi | Recommended name: Contryphan-Lo1 PublicationAlternative name(s): Contryphan-Lo1Curated Lo9592 Publications Cleaved into the following chain: Contryphan-Lo Lo9021 Publication |
Organismi | Conus buxeus loroisii (Cone snail) (Conus loroisii) |
Taxonomic identifieri | 410709 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Neogastropoda › Conoidea › Conidae › Conus › Dendroconus › |
Organism-specific databases
ConoServeri | 1272 Contryphan-Lo |
Subcellular locationi
Extracellular region or secreted
- Secreted 2 Publications
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 57 | P → V: Abolishes the very slow conformation equilibrium. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
PropeptideiPRO_0000445123 | 24 – 54 | CuratedAdd BLAST | 31 | |
PeptideiPRO_0000262670 | 55 – 62 | Contryphan-Lo3 Publications | 8 | |
PeptideiPRO_0000439694 | 56 – 62 | Contryphan-Lo Lo9021 Publication | 7 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 56 ↔ 62 | 3 PublicationsImported | ||
Modified residuei | 58 | D-tryptophan1 Publication1 Publication | 1 | |
Modified residuei | 62 | Cysteine amide2 Publications | 1 |
Keywords - PTMi
Amidation, D-amino acid, Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom duct.2 Publications
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2M6G | NMR | - | A | 55-62 | [»] | |
2M6H | NMR | - | A | 55-62 | [»] | |
SMRi | P0C250 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Domaini
The cysteine framework is C-C.Curated
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
InterProi | View protein in InterPro IPR004214 Conotoxin IPR011062 Contryphan_CS |
Pfami | View protein in Pfam PF02950 Conotoxin, 1 hit |
PROSITEi | View protein in PROSITE PS60027 CONTRYPHAN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0C250-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGKLTILVLV AAVLLSTQVM VQGDGDQPAD RNAVRRDDNP GGTRGRFMNI
60
LRRTGCPWDP WCG
Mass spectrometryi
Molecular mass is 959.35 Da from positions 55 - 62. Determined by MALDI. Monoisotopic mass.1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | KT713759 mRNA Translation: ALI96902.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | KT713759 mRNA Translation: ALI96902.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2M6G | NMR | - | A | 55-62 | [»] | |
2M6H | NMR | - | A | 55-62 | [»] | |
SMRi | P0C250 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Organism-specific databases
ConoServeri | 1272 Contryphan-Lo |
Family and domain databases
InterProi | View protein in InterPro IPR004214 Conotoxin IPR011062 Contryphan_CS |
Pfami | View protein in Pfam PF02950 Conotoxin, 1 hit |
PROSITEi | View protein in PROSITE PS60027 CONTRYPHAN, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | COW1_CONBL | |
Accessioni | P0C250Primary (citable) accession number: P0C250 Secondary accession number(s): A0A0P0CT07 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2006 |
Last sequence update: | October 10, 2018 | |
Last modified: | February 13, 2019 | |
This is version 42 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references