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Protein

Alpha-conotoxin VxXXA

Gene
N/A
Organism
Conus vexillum (Flag cone)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin specifically blocks mammalian neuronal nAChR of the alpha-7/CHRNA7, alpha-3-beta-2/CHRNA3-CHRNB2 (IC50=370 nM) and alpha-4-beta-2/CHRNA4-CHRNB2 subtypes. VxXXB inhibits alpha-7/CHRNA7 and alpha-3-beta-2/CHRNA3-CHRNB2 nAChR subtypes with the highest efficiency, followed by VxXXA and VxXXC. VxXXB and VxXXC inhibit the alpha-4-beta-2/CHRNA4-CHRNB2 nAChR subtype more efficiently than VxXXA.1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin VxXXA1 Publication
Alternative name(s):
Vx20.1Imported
VxXIIA1 Publication
OrganismiConus vexillum (Flag cone)
Taxonomic identifieri89431 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaNeogastropodaConoideaConidaeConusRhizoconus

Organism-specific databases

ConoServeri1686 VxXXA
3630 VxXXA precursor

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000039178225 – 451 PublicationAdd BLAST21
ChainiPRO_000024979346 – 92Alpha-conotoxin VxXXA1 PublicationAdd BLAST47

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei554-hydroxyproline; partial1 Publication1
Modified residuei704-hydroxyproline; partial1 Publication1
Modified residuei744-hydroxyproline; partial1 Publication1

Post-translational modificationi

Contains 5 disulfide bonds.Curated
VxXXA stands for the form with the Pro-55 hydroxylated. A second major form has both Pro-55 and Pro-74 hydroxylated. The two major forms VxXXA and [hydroxyPro-74]VxXXA exist in a 1:1 ratio.1 Publication
Minor forms are [hydroxyPro-70,hydroxyPro-74]VxXXA and [Pro-55]VxXXA.1 Publication

Keywords - PTMi

Disulfide bond, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom duct.1 Publication

Interactioni

Subunit structurei

Homodimer or pseudo-homodimer. Three dimers exist: homodimer of VxXXA, pseudo-homodimer of both VxXXA and [hydroxyPro-74]VxXXA and homodimer of [hydroxyPro-74]VxXXA. These three components exist in a 1:2:1 ratio.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP0C1W5
SMRiP0C1W5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The cysteine framework is XX (C-CC-C-CC-C-C-C-C).Curated

Sequence similaritiesi

Belongs to the conotoxin D superfamily.Curated

Keywords - Domaini

Signal

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C1W5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPKLEMMLLV LLIFPLSYFI AAGGQVVQVD RRGDGLAGYL QRGDRDVQDC
60 70 80 90
QVSTPGSKWG RCCLNRVCGP MCCPASHCYC VYHRGRGHGC SC
Length:92
Mass (Da):10,113
Last modified:March 2, 2010 - v2
Checksum:iEF52B139C26F83E1
GO

Mass spectrometryi

Molecular mass is 5134.6 Da from positions 46 - 92. Determined by ESI. With hydroxyPro-55.1 Publication
Molecular mass is 5150.6 Da from positions 46 - 92. Determined by ESI. With hydroxyPro-55 and hydroxyPro-74.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN178633 mRNA Translation: CAX51119.1

Similar proteinsi

Entry informationi

Entry nameiCDKA_CONVX
AccessioniPrimary (citable) accession number: P0C1W5
Secondary accession number(s): C4PWC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 2, 2010
Last modified: September 12, 2018
This is version 31 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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