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Protein

Alpha-conotoxin AnIB

Gene
N/A
Organism
Conus anemone (Anemone cone)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha-conotoxin AnIB: Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This synthetic toxin blocks rat alpha-3beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.3 nM) and alpha-7/CHRNA7 nAChRs (IC50=76 nM).1 Publication
Alpha-conotoxin AnIA: this synthetic toxin blocks rat alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.2 nM).1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin AnIB1 Publication
Cleaved into the following chain:
Alpha-conotoxin AnIA1 Publication
OrganismiConus anemone (Anemone cone)
Taxonomic identifieri101285 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaNeogastropodaConoideaConidaeConusFloraconus

Organism-specific databases

ConoServeri15 AnIB

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1G → GG: 1.4-fold increase in inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2. 1 Publication1
Mutagenesisi1Missing : 1.6-fold increase in inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00002497801 – 17Alpha-conotoxin AnIB1 PublicationAdd BLAST17
PeptideiPRO_00002497813 – 17Alpha-conotoxin AnIA1 PublicationAdd BLAST15

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi3 ↔ 9By similarity
Disulfide bondi4 ↔ 17By similarity
Modified residuei16Sulfotyrosine1 Publication1
Modified residuei17Cysteine amide1 Publication1

Post-translational modificationi

Amidation at Cys-17 is important for activity, since suppression of this modification induces a 1.9-fold decrease in inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.54 nM), and a 4.8-fold decrease in inhibitory potency on alpha-7/CHRNA7 nAChRs (IC50=367 nM).1 Publication
Sulfation at Tyr-16 is important for activity, since suppression of this modification induces a 2.3-fold decrease in inhibitory potency on alpha-3-beta-2/CHRNA3-CHRNB2 nAChRs (IC50=0.64 nM), and a 11-fold decrease in inhibitory potency on alpha-7/CHRNA7 nAChRs (IC50=836 nM).1 Publication

Keywords - PTMi

Amidation, Disulfide bond, Sulfation

Expressioni

Tissue specificityi

Expressed by the venom duct.1 Publication

Family & Domainsi

Domaini

The cysteine framework is I (CC-C-C). Alpha4/7 pattern.Curated

Sequence similaritiesi

Belongs to the conotoxin A superfamily.Curated

Family and domain databases

InterProiView protein in InterPro
IPR018072 Conotoxin_a-typ_CS
PROSITEiView protein in PROSITE
PS60014 ALPHA_CONOTOXIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Length:17
Mass (Da):1,714
Last modified:September 19, 2006 - v1
Checksum:i76F84AFDFAC99005
GO

Mass spectrometryi

Molecular mass is 1787.4 Da from positions 1 - 17. Determined by ESI. 1 Publication
Molecular mass is 1673.4 Da from positions 3 - 17. Determined by ESI. 1 Publication

Similar proteinsi

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ConoServeri15 AnIB

Family and domain databases

InterProiView protein in InterPro
IPR018072 Conotoxin_a-typ_CS
PROSITEiView protein in PROSITE
PS60014 ALPHA_CONOTOXIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCA1B_CONAN
AccessioniPrimary (citable) accession number: P0C1V7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: September 12, 2018
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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