UniProtKB - P0C1V0 (RLMH_STAAU)
Protein
Ribosomal RNA large subunit methyltransferase H
Gene
rlmH
Organism
Staphylococcus aureus
Status
Functioni
Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA (By similarity). Specific for fully assembled 70S ribosomes (PubMed:23150671).UniRule annotation1 Publication
Catalytic activityi
- pseudouridine1915 in 23S rRNA + S-adenosyl-L-methionine = H+ + N3-methylpseudouridine1915 in 23S rRNA + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.177UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 76 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 108 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotationCombined sources1 Publication | 1 |
GO - Molecular functioni
- rRNA (pseudouridine-N3-)-methyltransferase activity Source: UniProtKB-UniRule
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA large subunit methyltransferase HUniRule annotation (EC:2.1.1.177UniRule annotation)Alternative name(s): 23S rRNA (pseudouridine1915-N3)-methyltransferaseUniRule annotation 23S rRNA m3Psi1915 methyltransferaseUniRule annotation rRNA (pseudouridine-N3-)-methyltransferase RlmHUniRule annotation |
Gene namesi | Name:rlmHUniRule annotation |
Organismi | Staphylococcus aureus |
Taxonomic identifieri | 1280 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 77 | E → A: 30% decrease in methyltransferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000198181 | 1 – 159 | Ribosomal RNA large subunit methyltransferase HAdd BLAST | 159 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0C1V0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0C1V0 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 127 – 132 | S-adenosyl-L-methionine bindingUniRule annotationCombined sources1 Publication | 6 |
Sequence similaritiesi
Belongs to the RNA methyltransferase RlmH family.UniRule annotation
Family and domain databases
Gene3Di | 3.40.1280.10, 1 hit |
HAMAPi | MF_00658, 23SrRNA_methyltr_H, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR003742, RlmH-like IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR33603, PTHR33603, 1 hit |
Pfami | View protein in Pfam PF02590, SPOUT_MTase, 1 hit |
PIRSFi | PIRSF004505, MT_bac, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00246, tRNA_RlmH_YbeA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0C1V0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKITILAVGK LKEKYWKQAI AEYEKRLGPY TKIDIIEVPD EKAPENMSDK
60 70 80 90 100
EIEQVKEKEG QRILAKIKPQ STVITLEIQG KMLSSEGLAQ ELNQRMTQGQ
110 120 130 140 150
SDFVFVIGGS NGLHKDVLQR SNYALSFSKM TFPHQMMRVV LIEQVYRAFK
IMRGEAYHK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF181950 Genomic DNA Translation: AAF85653.1 D86934 Genomic DNA Translation: BAA82243.1 AB033763 Genomic DNA Translation: BAA86653.1 AB037671 Genomic DNA Translation: BAB47681.1 AB038513 Genomic DNA Translation: BAC53844.1 AB047089 Genomic DNA Translation: BAC57492.1 AB047239 Genomic DNA Translation: BAB47141.1 AB063172 Genomic DNA Translation: BAB72121.1 AB063173 Genomic DNA Translation: BAB72138.1 AB096217 Genomic DNA Translation: BAC67575.1 |
RefSeqi | WP_000704775.1, NZ_WYDB01000001.1 |
Genome annotation databases
GeneIDi | 45573363 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF181950 Genomic DNA Translation: AAF85653.1 D86934 Genomic DNA Translation: BAA82243.1 AB033763 Genomic DNA Translation: BAA86653.1 AB037671 Genomic DNA Translation: BAB47681.1 AB038513 Genomic DNA Translation: BAC53844.1 AB047089 Genomic DNA Translation: BAC57492.1 AB047239 Genomic DNA Translation: BAB47141.1 AB063172 Genomic DNA Translation: BAB72121.1 AB063173 Genomic DNA Translation: BAB72138.1 AB096217 Genomic DNA Translation: BAC67575.1 |
RefSeqi | WP_000704775.1, NZ_WYDB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1VH0 | X-ray | 2.31 | A/B/C/D/E/F | 2-159 | [»] | |
4FAK | X-ray | 1.70 | A | 1-159 | [»] | |
SMRi | P0C1V0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
GeneIDi | 45573363 |
Miscellaneous databases
EvolutionaryTracei | P0C1V0 |
Family and domain databases
Gene3Di | 3.40.1280.10, 1 hit |
HAMAPi | MF_00658, 23SrRNA_methyltr_H, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR003742, RlmH-like IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR33603, PTHR33603, 1 hit |
Pfami | View protein in Pfam PF02590, SPOUT_MTase, 1 hit |
PIRSFi | PIRSF004505, MT_bac, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00246, tRNA_RlmH_YbeA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RLMH_STAAU | |
Accessioni | P0C1V0Primary (citable) accession number: P0C1V0 Secondary accession number(s): P0A0N8, Q9WVW7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | September 5, 2006 | |
Last modified: | December 2, 2020 | |
This is version 77 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families