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Protein

Transcriptional regulator SarA

Gene

sarA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Global regulator with both positive and negative effects that controls expression of several virulence factors and biofilm formation process in a cell density-dependent manner. In a strain-dependent manner plays a role in multidrug resistance mechanism. Is required for transcription of primary transcripts RNAII and RNAIII generated by agr (virulence accessory gene regulator) locus. Acts as a transcriptional activator of the genes encoding, among others, for fibronectin binding proteins (fnbA and fnbB), hemolysins (hla, hld, hlgB and hlgC), serine proteases (splA, splB, splD and splF) and of the bap gene, which is essential for biofilm development in some strains. Negatively regulates the expression of the genes for protein A (spa), lipase (lip), thermonuclease (nuc), immunodominant staphylococcal antigen B (isaB), staphylococcal serine and cysteine proteases (sspA and sspB), staphostatin B (sspC), metalloprotease aureolysin (aur) and collagen adhesin (cna). Probably activates the development of biofilm by both enhancing the ica operon transcription and suppressing the transcription of either a protein involved in the turnover of PIA/PNAG or a repressor of its synthesis, whose expression would be sigma-B-dependent.1 Publication

Miscellaneous

Can regulate transcription of some virulence factors (hemolysins, staphylococcal serine and cysteine proteases, lipase, protein A and metalloprotease aureolysin) via either agr-dependent and/or agr-independent pathway. Agr and SarA have opposing effects on the expression of sspA, sspB, sspC, lip and aur.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Divalent metal cationBy similarity1
Metal bindingi8Divalent metal cationBy similarity1
Metal bindingi11Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: InterPro
  • single-species submerged biofilm formation Source: CACAO

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation, Virulence
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulator SarA
Alternative name(s):
Staphylococcal accessory regulator A
Gene namesi
Name:sarA
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002195762 – 124Transcriptional regulator SarAAdd BLAST123

Expressioni

Inductioni

Expressed at the exponential growth phase and maximally induced during the transition from late exponential phase to stationary phase. Repressed by SarR and activated by two-component regulatory system ArlS/ArlR. Activated by SigB in strains harboring an intact sigB operon (rsbU, rsbV, rsbW, and sigB). Transcription is also attenuated by MsrR.

Interactioni

Subunit structurei

Homodimer.2 Publications

Structurei

Secondary structure

1124
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0C1U6
SMRiP0C1U6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C1U6

Family & Domainsi

Sequence similaritiesi

Belongs to the SarA family.Curated

Phylogenomic databases

eggNOGiCOG1846 LUCA

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR010166 SarA/Rot
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
SUPFAMiSSF46785 SSF46785, 1 hit
TIGRFAMsiTIGR01889 Staph_reg_Sar, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C1U6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAITKINDCF ELLSMVTYAD KLKSLIKKEF SISFEEFAVL TYISENKEKE
60 70 80 90 100
YYLKDIINHL NYKQPQVVKA VKILSQEDYF DKKRNEHDER TVLILVNAQQ
110 120
RKKIESLLSR VNKRITEANN EIEL
Length:124
Mass (Da):14,718
Last modified:January 23, 2007 - v2
Checksum:iDB9A16E806C10661
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515775 Genomic DNA Translation: AAM74164.1
DQ414117 Genomic DNA Translation: ABD73686.1
DQ414116 Genomic DNA Translation: ABD73685.1
DQ414115 Genomic DNA Translation: ABD73684.1
DQ414114 Genomic DNA Translation: ABD73683.1
DQ414113 Genomic DNA Translation: ABD73682.1
DQ414112 Genomic DNA Translation: ABD73681.1
DQ414111 Genomic DNA Translation: ABD73680.1
DQ414110 Genomic DNA Translation: ABD73679.1
DQ414109 Genomic DNA Translation: ABD73678.1
DQ414108 Genomic DNA Translation: ABD73677.1
DQ414107 Genomic DNA Translation: ABD73676.1
DQ414106 Genomic DNA Translation: ABD73675.1
DQ414105 Genomic DNA Translation: ABD73674.1
DQ414104 Genomic DNA Translation: ABD73673.1
DQ414103 Genomic DNA Translation: ABD73672.1
DQ414102 Genomic DNA Translation: ABD73671.1
DQ414101 Genomic DNA Translation: ABD73670.1
DQ414100 Genomic DNA Translation: ABD73669.1
DQ414099 Genomic DNA Translation: ABD73668.1
DQ414098 Genomic DNA Translation: ABD73667.1
DQ414097 Genomic DNA Translation: ABD73666.1
DQ414096 Genomic DNA Translation: ABD73665.1
DQ414095 Genomic DNA Translation: ABD73664.1
DQ414094 Genomic DNA Translation: ABD73663.1
DQ414093 Genomic DNA Translation: ABD73662.1
DQ414092 Genomic DNA Translation: ABD73661.1
DQ414091 Genomic DNA Translation: ABD73660.1
DQ414090 Genomic DNA Translation: ABD73659.1
DQ414089 Genomic DNA Translation: ABD73658.1
RefSeqiWP_001018677.1, NZ_UHEH01000001.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF515775 Genomic DNA Translation: AAM74164.1
DQ414117 Genomic DNA Translation: ABD73686.1
DQ414116 Genomic DNA Translation: ABD73685.1
DQ414115 Genomic DNA Translation: ABD73684.1
DQ414114 Genomic DNA Translation: ABD73683.1
DQ414113 Genomic DNA Translation: ABD73682.1
DQ414112 Genomic DNA Translation: ABD73681.1
DQ414111 Genomic DNA Translation: ABD73680.1
DQ414110 Genomic DNA Translation: ABD73679.1
DQ414109 Genomic DNA Translation: ABD73678.1
DQ414108 Genomic DNA Translation: ABD73677.1
DQ414107 Genomic DNA Translation: ABD73676.1
DQ414106 Genomic DNA Translation: ABD73675.1
DQ414105 Genomic DNA Translation: ABD73674.1
DQ414104 Genomic DNA Translation: ABD73673.1
DQ414103 Genomic DNA Translation: ABD73672.1
DQ414102 Genomic DNA Translation: ABD73671.1
DQ414101 Genomic DNA Translation: ABD73670.1
DQ414100 Genomic DNA Translation: ABD73669.1
DQ414099 Genomic DNA Translation: ABD73668.1
DQ414098 Genomic DNA Translation: ABD73667.1
DQ414097 Genomic DNA Translation: ABD73666.1
DQ414096 Genomic DNA Translation: ABD73665.1
DQ414095 Genomic DNA Translation: ABD73664.1
DQ414094 Genomic DNA Translation: ABD73663.1
DQ414093 Genomic DNA Translation: ABD73662.1
DQ414092 Genomic DNA Translation: ABD73661.1
DQ414091 Genomic DNA Translation: ABD73660.1
DQ414090 Genomic DNA Translation: ABD73659.1
DQ414089 Genomic DNA Translation: ABD73658.1
RefSeqiWP_001018677.1, NZ_UHEH01000001.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FZPX-ray2.95B/D2-124[»]
ProteinModelPortaliP0C1U6
SMRiP0C1U6
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG1846 LUCA

Miscellaneous databases

EvolutionaryTraceiP0C1U6
PROiPR:P0C1U6

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR010166 SarA/Rot
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
SUPFAMiSSF46785 SSF46785, 1 hit
TIGRFAMsiTIGR01889 Staph_reg_Sar, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSARA_STAAU
AccessioniPrimary (citable) accession number: P0C1U6
Secondary accession number(s): Q200K0, Q53600, Q53777
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 73 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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