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Protein

Wee1-like protein kinase 2

Gene

WEE2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei241ATPPROSITE-ProRule annotation1
Active sitei339Proton acceptorPROSITE-ProRule annotation1
Metal bindingi344Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi380Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi218 – 226ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
Biological processMeiosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiP0C1S8

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Gene namesi
Name:WEE2
Synonyms:WEE1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000214102.7
HGNCiHGNC:19684 WEE2
MIMi614084 gene
neXtProtiNX_P0C1S8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000214102
PharmGKBiPA134909633

Chemistry databases

ChEMBLiCHEMBL5095

Polymorphism and mutation databases

BioMutaiWEE2
DMDMi254763343

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002480791 – 567Wee1-like protein kinase 2Add BLAST567

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei76PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation leads to increase its activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0C1S8
PeptideAtlasiP0C1S8
PRIDEiP0C1S8
ProteomicsDBi52298

PTM databases

iPTMnetiP0C1S8
PhosphoSitePlusiP0C1S8

Expressioni

Tissue specificityi

Expressed in testis.1 Publication

Gene expression databases

BgeeiENSG00000214102 Expressed in 64 organ(s), highest expression level in intestine
CleanExiHS_WEE2
ExpressionAtlasiP0C1S8 baseline and differential

Organism-specific databases

HPAiHPA027418

Interactioni

Protein-protein interaction databases

BioGridi138978, 1 interactor
STRINGi9606.ENSP00000380675

Chemistry databases

BindingDBiP0C1S8

Structurei

Secondary structure

1567
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0C1S8
SMRiP0C1S8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini212 – 486Protein kinasePROSITE-ProRule annotationAdd BLAST275

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili494 – 519Sequence analysisAdd BLAST26

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi173 – 175Nuclear localization signalBy similarity3
Motifi315 – 329Nuclear export signalBy similarityAdd BLAST15

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0601 Eukaryota
ENOG410XS1M LUCA
GeneTreeiENSGT00530000063230
HOGENOMiHOG000004824
HOVERGENiHBG005050
InParanoidiP0C1S8
KOiK06632
OMAiDWFLSAN
OrthoDBiEOG091G049H
PhylomeDBiP0C1S8
TreeFamiTF101088

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR017164 Wee1-like_protein_kinase
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF037281 Wee1-like_protein_kinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P0C1S8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDKDIDKEL RQKLNFSYCE ETEIEGQKKV EESREASSQT PEKGEVQDSE
60 70 80 90 100
AKGTPPWTPL SNVHELDTSS EKDKESPDQI LRTPVSHPLK CPETPAQPDS
110 120 130 140 150
RSKLLPSDSP STPKTMLSRL VISPTGKLPS RGPKHLKLTP APLKDEMTSL
160 170 180 190 200
ALVNINPFTP ESYKKLFLQS GGKRKIRGDL EEAGPEEGKG GLPAKRCVLR
210 220 230 240 250
ETNMASRYEK EFLEVEKIGV GEFGTVYKCI KRLDGCVYAI KRSMKTFTEL
260 270 280 290 300
SNENSALHEV YAHAVLGHHP HVVRYYSSWA EDDHMIIQNE YCNGGSLQAA
310 320 330 340 350
ISENTKSGNH FEEPKLKDIL LQISLGLNYI HNSSMVHLDI KPSNIFICHK
360 370 380 390 400
MQSESSGVIE EVENEADWFL SANVMYKIGD LGHATSINKP KVEEGDSRFL
410 420 430 440 450
ANEILQEDYR HLPKADIFAL GLTIAVAAGA ESLPTNGAAW HHIRKGNFPD
460 470 480 490 500
VPQELSESFS SLLKNMIQPD AEQRPSAAAL ARNTVLRPSL GKTEELQQQL
510 520 530 540 550
NLEKFKTATL ERELREAQQA QSPQGYTHHG DTGVSGTHTG SRSTKRLVGG
560
KSARSSSFTS GEREPLH
Length:567
Mass (Da):62,925
Last modified:July 28, 2009 - v2
Checksum:iBC05DDFE96D87E3F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7C5P0H7C5P0_HUMAN
Wee1-like protein kinase 2
WEE2
203Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti351M → V in AK131218 (PubMed:14702039).Curated1
Sequence conflicti529H → Y in AK131218 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0413048K → T1 PublicationCorresponds to variant dbSNP:rs35672788Ensembl.1
Natural variantiVAR_041305332N → K in a gastric adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041306398R → H in an ovarian mucinous carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs200610853Ensembl.1
Natural variantiVAR_041307470D → E1 PublicationCorresponds to variant dbSNP:rs55901099Ensembl.1
Natural variantiVAR_041308526Y → D1 PublicationCorresponds to variant dbSNP:rs35683659Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK131218 mRNA No translation available.
AC004918 Genomic DNA No translation available.
CCDSiCCDS43660.1
RefSeqiNP_001099028.1, NM_001105558.1
UniGeneiHs.657927

Genome annotation databases

EnsembliENST00000397541; ENSP00000380675; ENSG00000214102
ENST00000574659; ENSP00000459392; ENSG00000263042
GeneIDi494551
KEGGihsa:494551
UCSCiuc003vwn.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK131218 mRNA No translation available.
AC004918 Genomic DNA No translation available.
CCDSiCCDS43660.1
RefSeqiNP_001099028.1, NM_001105558.1
UniGeneiHs.657927

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5VDKX-ray2.70A202-492[»]
ProteinModelPortaliP0C1S8
SMRiP0C1S8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi138978, 1 interactor
STRINGi9606.ENSP00000380675

Chemistry databases

BindingDBiP0C1S8
ChEMBLiCHEMBL5095

PTM databases

iPTMnetiP0C1S8
PhosphoSitePlusiP0C1S8

Polymorphism and mutation databases

BioMutaiWEE2
DMDMi254763343

Proteomic databases

PaxDbiP0C1S8
PeptideAtlasiP0C1S8
PRIDEiP0C1S8
ProteomicsDBi52298

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397541; ENSP00000380675; ENSG00000214102
ENST00000574659; ENSP00000459392; ENSG00000263042
GeneIDi494551
KEGGihsa:494551
UCSCiuc003vwn.2 human

Organism-specific databases

CTDi494551
EuPathDBiHostDB:ENSG00000214102.7
GeneCardsiWEE2
H-InvDBiHIX0007154
HIX0033848
HGNCiHGNC:19684 WEE2
HPAiHPA027418
MIMi614084 gene
neXtProtiNX_P0C1S8
OpenTargetsiENSG00000214102
PharmGKBiPA134909633
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0601 Eukaryota
ENOG410XS1M LUCA
GeneTreeiENSGT00530000063230
HOGENOMiHOG000004824
HOVERGENiHBG005050
InParanoidiP0C1S8
KOiK06632
OMAiDWFLSAN
OrthoDBiEOG091G049H
PhylomeDBiP0C1S8
TreeFamiTF101088

Enzyme and pathway databases

SignaLinkiP0C1S8

Miscellaneous databases

GenomeRNAii494551
PROiPR:P0C1S8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000214102 Expressed in 64 organ(s), highest expression level in intestine
CleanExiHS_WEE2
ExpressionAtlasiP0C1S8 baseline and differential

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR017164 Wee1-like_protein_kinase
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF037281 Wee1-like_protein_kinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWEE2_HUMAN
AccessioniPrimary (citable) accession number: P0C1S8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 28, 2009
Last modified: September 12, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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