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UniProtKB - P0C1A9 (PMEA_DICD3)
Protein
Pectinesterase A
Gene
pemA
Organism
Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))
Status
Functioni
Catalyzes the first step in maceration and soft-rotting of plant tissue.
2 PublicationsCatalytic activityi
- [(1→4)-α-D-galacturonosyl methyl ester](n) + n H2O = [(1→4)-α-D-galacturonosyl](n) + n H+ + n methanol2 PublicationsEC:3.1.1.112 Publications
: pectin degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.2 Publications This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 109 | Substrate1 Publication | 1 | |
Binding sitei | 153 | Substrate1 Publication | 1 | |
Sitei | 177 | Transition state stabilizer1 Publication | 1 | |
Active sitei | 178 | Proton donor1 Publication | 1 | |
Active sitei | 199 | Nucleophile1 Publication | 1 | |
Binding sitei | 219 | Substrate1 Publication | 1 | |
Binding sitei | 226 | Substrate1 Publication | 1 | |
Binding sitei | 230 | Substrate1 Publication | 1 | |
Binding sitei | 267 | Substrate1 Publication | 1 | |
Binding sitei | 269 | Substrate1 Publication | 1 | |
Binding sitei | 272 | Substrate; via amide nitrogen and carbonyl oxygen1 Publication | 1 |
GO - Molecular functioni
- aspartyl esterase activity Source: UniProtKB-KW
- pectinesterase activity Source: ASAP
GO - Biological processi
- cell wall modification Source: InterPro
- pectin catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Aspartyl esterase, Hydrolase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BioCyci | DDAD198628:DDA3937_RS15630-MONOMER |
UniPathwayi | UPA00545;UER00823 |
Names & Taxonomyi
Protein namesi | Recommended name: Pectinesterase A (EC:3.1.1.111 Publication)Short name: PE A Alternative name(s): Pectin methylesterase A Short name: PME1 Publication |
Gene namesi | Name:pemA1 Publication Synonyms:pem1 Publication Ordered Locus Names:Dda3937_03374 |
Organismi | Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) |
Taxonomic identifieri | 198628 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Pectobacteriaceae › Dickeya › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Note: Upon overexpression also accumulates in the periplasm.1 Publication
Extracellular region or secreted
- extracellular space Source: ASAP
Keywords - Cellular componenti
SecretedPathology & Biotechi
Disruption phenotypei
Decreased growth on methylated oligogalacturides; double pemA-pemB deletions grow very poorly on methylated oligogalacturides.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 153 | Q → A: Strong decrease in affinity for substrate. 1 Publication | 1 | |
Mutagenesisi | 177 | Q → A: Strong decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 178 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 198 | V → A: Strong decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 199 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 267 | R → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 269 | W → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 272 | T → A: Decreases affinity for substrate. 1 Publication | 1 | |
Mutagenesisi | 306 | M → A: Strong decrease in catalytic activity. Decreases affinity for substrate. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | 1 PublicationAdd BLAST | 24 | |
ChainiPRO_0000233033 | 25 – 366 | Pectinesterase AAdd BLAST | 342 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 192 ↔ 212 | 1 Publication |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Monomer.
2 PublicationsProtein-protein interaction databases
STRINGi | 198628.Dda3937_03374 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0C1A9 |
SMRi | P0C1A9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0C1A9 |
Family & Domainsi
Sequence similaritiesi
Belongs to the pectinesterase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG4677, Bacteria |
HOGENOMi | CLU_012243_3_1_6 |
OMAi | NRVAWCR |
OrthoDBi | 1095798at2 |
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat |
Pfami | View protein in Pfam PF01095, Pectinesterase, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS00503, PECTINESTERASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0C1A9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKTISGTLA LSLIIAASVH QAQAATTYNA VVSKSSSDGK TFKTIADAIA
60 70 80 90 100
SAPAGSTPFV ILIKNGVYNE RLTITRNNLH LKGESRNGAV IAAATAAGTL
110 120 130 140 150
KSDGSKWGTA GSSTITISAK DFSAQSLTIR NDFDFPANQA KSDSDSSKIK
160 170 180 190 200
DTQAVALYVT KSGDRAYFKD VSLVGYQDTL YVSGGRSFFS DCRISGTVDF
210 220 230 240 250
IFGDGTALFN NCDLVSRYRA DVKSGNVSGY LTAPSTNINQ KYGLVITNSR
260 270 280 290 300
VIRESDSVPA KSYGLGRPWH PTTTFSDGRY ADPNAIGQTV FLNTSMDNHI
310 320 330 340 350
YGWDKMSGKD KNGNTIWFNP EDSRFFEYKS YGAGATVSKD RRQLTDAQAA
360
EYTQSKVLGD WTPTLP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07644 Genomic DNA Translation: AAA24852.1 CP002038 Genomic DNA Translation: ADM99554.1 |
PIRi | JN0799 |
RefSeqi | WP_013318985.1, NC_014500.1 |
Genome annotation databases
EnsemblBacteriai | ADM99554; ADM99554; Dda3937_03374 |
GeneIDi | 9734805 |
KEGGi | ddd:Dda3937_03374 |
PATRICi | fig|198628.6.peg.3312 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07644 Genomic DNA Translation: AAA24852.1 CP002038 Genomic DNA Translation: ADM99554.1 |
PIRi | JN0799 |
RefSeqi | WP_013318985.1, NC_014500.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2NSP | X-ray | 1.70 | A/B | 25-366 | [»] | |
2NST | X-ray | 1.70 | A/B | 25-366 | [»] | |
2NT6 | X-ray | 1.70 | A/B | 25-366 | [»] | |
2NT9 | X-ray | 1.90 | A/B | 25-366 | [»] | |
2NTB | X-ray | 1.80 | A/B | 25-366 | [»] | |
2NTP | X-ray | 1.70 | A/B | 25-366 | [»] | |
2NTQ | X-ray | 1.80 | A/B | 25-366 | [»] | |
AlphaFoldDBi | P0C1A9 | |||||
SMRi | P0C1A9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 198628.Dda3937_03374 |
Genome annotation databases
EnsemblBacteriai | ADM99554; ADM99554; Dda3937_03374 |
GeneIDi | 9734805 |
KEGGi | ddd:Dda3937_03374 |
PATRICi | fig|198628.6.peg.3312 |
Phylogenomic databases
eggNOGi | COG4677, Bacteria |
HOGENOMi | CLU_012243_3_1_6 |
OMAi | NRVAWCR |
OrthoDBi | 1095798at2 |
Enzyme and pathway databases
UniPathwayi | UPA00545;UER00823 |
BioCyci | DDAD198628:DDA3937_RS15630-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0C1A9 |
Family and domain databases
Gene3Di | 2.160.20.10, 1 hit |
InterProi | View protein in InterPro IPR012334, Pectin_lyas_fold IPR011050, Pectin_lyase_fold/virulence IPR033131, Pectinesterase_Asp_AS IPR000070, Pectinesterase_cat |
Pfami | View protein in Pfam PF01095, Pectinesterase, 1 hit |
SUPFAMi | SSF51126, SSF51126, 1 hit |
PROSITEi | View protein in PROSITE PS00503, PECTINESTERASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PMEA_DICD3 | |
Accessioni | P0C1A9Primary (citable) accession number: P0C1A9 Secondary accession number(s): E0SAZ5, P07863 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 18, 2006 |
Last sequence update: | April 18, 2006 | |
Last modified: | May 25, 2022 | |
This is version 81 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families