UniProtKB - P0C0U4 (RIMK_ECOLI)
Protein
Ribosomal protein S6--L-glutamate ligase
Gene
rimK
Organism
Escherichia coli (strain K12)
Status
Functioni
Is an L-glutamate ligase that catalyzes the ATP-dependent post-translational addition of glutamate residues to the C-terminus of ribosomal protein S6 (RpsF). Is also able to catalyze the synthesis of poly-alpha-glutamate in vitro, via ATP hydrolysis from unprotected glutamate as substrate. The number of glutamate residues added to either RpsF or to poly-alpha-glutamate changes with pH.UniRule annotation2 Publications
Cofactori
Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
pH dependencei
Optimum pH is 9.5 for RpsF modification and 9 for polyglutamate synthase activity.1 Publication
Temperature dependencei
Shows thermal stability. Exhibits 86% activity after incubation at 55 degrees Celsius for 15 minutes, but its activity decreases sharply at 60 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 141 | ATP1 Publication | 1 | |
Binding sitei | 187 | ATP1 Publication | 1 | |
Metal bindingi | 248 | Magnesium or manganese 1UniRule annotation | 1 | |
Metal bindingi | 260 | Magnesium or manganese 1UniRule annotation | 1 | |
Metal bindingi | 260 | Magnesium or manganese 2UniRule annotation | 1 | |
Metal bindingi | 262 | Magnesium or manganese 2UniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 178 – 179 | ATP1 Publication | 2 | |
Nucleotide bindingi | 211 – 213 | ATP1 Publication | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- identical protein binding Source: EcoCyc
- ligase activity, forming carbon-nitrogen bonds Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- ribosomal S6-glutamic acid ligase activity Source: EcoCyc
GO - Biological processi
- C-terminal protein amino acid modification Source: EcoCyc
- SOS response Source: EcoCyc
- translation Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10852-MONOMER MetaCyc:EG10852-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal protein S6--L-glutamate ligaseUniRule annotation (EC:6.3.2.-UniRule annotation)Alternative name(s): Polyglutamate synthase Ribosomal protein S6 modification proteinUniRule annotation |
Gene namesi | Name:rimK Ordered Locus Names:b0852, JW0836 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Biotechnological usei
This protein may find application in fermentative methods that use microorganisms overexpressing rimK for mass production of poly-alpha-amino acids, which is thought to be the most economical and ecofriendly manufacturing process.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205455 | 1 – 300 | Ribosomal protein S6--L-glutamate ligaseAdd BLAST | 300 |
Proteomic databases
jPOSTi | P0C0U4 |
PaxDbi | P0C0U4 |
PRIDEi | P0C0U4 |
Interactioni
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4259992, 31 interactors |
IntActi | P0C0U4, 4 interactors |
STRINGi | 511145.b0852 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0C0U4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 287 | ATP-graspUniRule annotationAdd BLAST | 184 |
Sequence similaritiesi
Belongs to the RimK family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0189, Bacteria |
HOGENOMi | CLU_054353_0_1_6 |
InParanoidi | P0C0U4 |
PhylomeDBi | P0C0U4 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_01552, RimK, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013651, ATP-grasp_RimK-type IPR013815, ATP_grasp_subdomain_1 IPR023533, RimK IPR041107, Rimk_N IPR004666, RpS6_RimK/Lys_biosynth_LsyX |
Pfami | View protein in Pfam PF08443, RimK, 1 hit PF18030, Rimk_N, 1 hit |
TIGRFAMsi | TIGR00768, rimK_fam, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
i Sequence
Sequence statusi: Complete.
P0C0U4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIAILSRDG TLYSCKRLRE AAIQRGHLVE ILDPLSCYMN INPAASSIHY
60 70 80 90 100
KGRKLPHFDA VIPRIGTAIT FYGTAALRQF EMLGSYPLNE SVAIARARDK
110 120 130 140 150
LRSMQLLARQ GIDLPVTGIA HSPDDTSDLI DMVGGAPLVV KLVEGTQGIG
160 170 180 190 200
VVLAETRQAA ESVIDAFRGL NAHILVQEYI KEAQGCDIRC LVVGDEVVAA
210 220 230 240 250
IERRAKEGDF RSNLHRGGAA SVASITPQER EIAIKAARTM ALDVAGVDIL
260 270 280 290 300
RANRGPLVME VNASPGLEGI EKTTGIDIAG KMIRWIERHA TTEYCLKTGG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 18 | MKIAI…SCKRL → MERSIRVSGW in CAA33868 (PubMed:2570347).CuratedAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15859 Genomic DNA Translation: CAA33868.1 U00096 Genomic DNA Translation: AAC73939.1 AP009048 Genomic DNA Translation: BAA35563.2 |
PIRi | D64823 |
RefSeqi | NP_415373.1, NC_000913.3 WP_000684321.1, NZ_STEB01000019.1 |
Genome annotation databases
EnsemblBacteriai | AAC73939; AAC73939; b0852 BAA35563; BAA35563; BAA35563 |
GeneIDi | 58460667 945484 |
KEGGi | ecj:JW0836 eco:b0852 |
PATRICi | fig|1411691.4.peg.1426 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15859 Genomic DNA Translation: CAA33868.1 U00096 Genomic DNA Translation: AAC73939.1 AP009048 Genomic DNA Translation: BAA35563.2 |
PIRi | D64823 |
RefSeqi | NP_415373.1, NC_000913.3 WP_000684321.1, NZ_STEB01000019.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4IWX | X-ray | 2.85 | A | 1-300 | [»] | |
4IWY | X-ray | 2.90 | A | 2-300 | [»] | |
5ZCT | X-ray | 2.05 | A/B/C/D/E/F/G/H | 1-292 | [»] | |
5ZK6 | X-ray | 3.15 | A | 1-300 | [»] | |
SMRi | P0C0U4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259992, 31 interactors |
IntActi | P0C0U4, 4 interactors |
STRINGi | 511145.b0852 |
Proteomic databases
jPOSTi | P0C0U4 |
PaxDbi | P0C0U4 |
PRIDEi | P0C0U4 |
Genome annotation databases
EnsemblBacteriai | AAC73939; AAC73939; b0852 BAA35563; BAA35563; BAA35563 |
GeneIDi | 58460667 945484 |
KEGGi | ecj:JW0836 eco:b0852 |
PATRICi | fig|1411691.4.peg.1426 |
Organism-specific databases
EchoBASEi | EB0845 |
Phylogenomic databases
eggNOGi | COG0189, Bacteria |
HOGENOMi | CLU_054353_0_1_6 |
InParanoidi | P0C0U4 |
PhylomeDBi | P0C0U4 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10852-MONOMER MetaCyc:EG10852-MONOMER |
Miscellaneous databases
PROi | PR:P0C0U4 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit |
HAMAPi | MF_01552, RimK, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013651, ATP-grasp_RimK-type IPR013815, ATP_grasp_subdomain_1 IPR023533, RimK IPR041107, Rimk_N IPR004666, RpS6_RimK/Lys_biosynth_LsyX |
Pfami | View protein in Pfam PF08443, RimK, 1 hit PF18030, Rimk_N, 1 hit |
TIGRFAMsi | TIGR00768, rimK_fam, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIMK_ECOLI | |
Accessioni | P0C0U4Primary (citable) accession number: P0C0U4 Secondary accession number(s): P17116, P75814 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families