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Protein

Histone H2A.Z

Gene

H2afz

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. Essential for early development.3 Publications

Miscellaneous

Down-regulation of H2afz by RNA interference leads to death at early embryonic stages.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-171306 Packaging Of Telomere Ends
R-MMU-201722 Formation of the beta-catenin:TCF transactivating complex
R-MMU-212300 PRC2 methylates histones and DNA
R-MMU-2299718 Condensation of Prophase Chromosomes
R-MMU-2559580 Oxidative Stress Induced Senescence
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-2559586 DNA Damage/Telomere Stress Induced Senescence
R-MMU-3214858 RMTs methylate histone arginines
R-MMU-427359 SIRT1 negatively regulates rRNA expression
R-MMU-427413 NoRC negatively regulates rRNA expression
R-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-5578749 Transcriptional regulation by small RNAs
R-MMU-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-MMU-73728 RNA Polymerase I Promoter Opening
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2A.Z
Short name:
H2A/z
Gene namesi
Name:H2afz
Synonyms:H2az
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1888388 H2afz

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00000552982 – 128Histone H2A.ZAdd BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5N6-acetyllysine; alternate1 Publication1
Modified residuei5N6-methyllysine; alternateBy similarity1
Modified residuei8N6-acetyllysine; alternate1 Publication1
Modified residuei8N6-methyllysine; alternateBy similarity1
Modified residuei12N6-acetyllysine1 Publication1
Modified residuei14N6-acetyllysineBy similarity1
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.By similarity
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.2 Publications
Not phosphorylated.1 Publication
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

EPDiP0C0S6
MaxQBiP0C0S6
PaxDbiP0C0S6
PRIDEiP0C0S6
TopDownProteomicsiP0C0S6

PTM databases

iPTMnetiP0C0S6
PhosphoSitePlusiP0C0S6

Expressioni

Gene expression databases

BgeeiENSMUSG00000037894 Expressed in 265 organ(s), highest expression level in primitive streak
CleanExiMM_H2AFZ
ExpressionAtlasiP0C0S6 baseline and differential
GenevisibleiP0C0S6 MM

Interactioni

Subunit structurei

Interacts (via M6 cassette) with ANP32E; leading to removal of H2A.Z/H2AFZ from the nucleosome (By similarity). The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms a heterodimer with H2B (By similarity). H2AFZ interacts with INCENP (PubMed:12660166). Interacts with VPS72 (via N-terminal domain). Interacts with PWWP2A (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206173, 5 interactors
IntActiP0C0S6, 13 interactors
MINTiP0C0S6
STRINGi10090.ENSMUSP00000036907

Structurei

3D structure databases

ProteinModelPortaliP0C0S6
SMRiP0C0S6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 17Required for interaction with INCENP1 PublicationAdd BLAST17
Regioni89 – 100M6 cassetteBy similarityAdd BLAST12
Regioni93 – 103Required for interaction with INCENP1 PublicationAdd BLAST11
Regioni120 – 128Required for interaction with PWWP2ABy similarity9

Sequence similaritiesi

Belongs to the histone H2A family.Curated

Phylogenomic databases

eggNOGiKOG1757 Eukaryota
COG5262 LUCA
GeneTreeiENSGT00900000140979
HOGENOMiHOG000234652
HOVERGENiHBG009342
InParanoidiP0C0S6
KOiK11251
OMAiDKDKGKM
OrthoDBiEOG091G0XGD
PhylomeDBiP0C0S6
TreeFamiTF354232

Family and domain databases

CDDicd00074 H2A, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR002119 Histone_H2A
IPR007125 Histone_H2A/H2B/H3
IPR032454 Histone_H2A_C
IPR032458 Histone_H2A_CS
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PF16211 Histone_H2A_C, 1 hit
PRINTSiPR00620 HISTONEH2A
SMARTiView protein in SMART
SM00414 H2A, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00046 HISTONE_H2A, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P0C0S6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGGKAGKDS GKAKTKAVSR SQRAGLQFPV GRIHRHLKSR TTSHGRVGAT
60 70 80 90 100
AAVYSAAILE YLTAEVLELA GNASKDLKVK RITPRHLQLA IRGDEELDSL
110 120
IKATIAGGGV IPHIHKSLIG KKGQQKTV
Length:128
Mass (Da):13,553
Last modified:January 23, 2007 - v2
Checksum:iE024E53818230371
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3UWL7G3UWL7_MOUSE
Histone H2A
H2afz
87Annotation score:
Q3UA95Q3UA95_MOUSE
Histone H2A
H2afz
105Annotation score:
G3UX40G3UX40_MOUSE
Histone H2A.Z
H2afz
54Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12K → E in BAE30460 (PubMed:16141072).Curated1
Sequence conflicti29P → A in BAE26959 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70494 mRNA Translation: AAB09578.1
AY074806 mRNA Translation: AAL71864.1
AK028176 mRNA Translation: BAC25791.1
AK088704 mRNA Translation: BAC40515.1
AK143572 mRNA Translation: BAE25443.1
AK146181 mRNA Translation: BAE26959.1
AK150454 mRNA Translation: BAE29574.1
AK151052 mRNA Translation: BAE30069.1
AK151509 mRNA Translation: BAE30460.1
AK151868 mRNA Translation: BAE30756.1
AK152398 mRNA Translation: BAE31186.1
AK152848 mRNA Translation: BAE31541.1
AK168178 mRNA Translation: BAE40138.1
BC079903 mRNA Translation: AAH79903.1
BC147478 mRNA Translation: AAI47479.1
BC158035 mRNA Translation: AAI58036.1
BC171999 mRNA Translation: AAI71999.1
CCDSiCCDS38647.1
RefSeqiNP_058030.1, NM_016750.3
UniGeneiMm.117541
Mm.372513
Mm.465508

Genome annotation databases

EnsembliENSMUST00000041045; ENSMUSP00000036907; ENSMUSG00000037894
GeneIDi51788
KEGGimmu:51788
UCSCiuc008rmo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70494 mRNA Translation: AAB09578.1
AY074806 mRNA Translation: AAL71864.1
AK028176 mRNA Translation: BAC25791.1
AK088704 mRNA Translation: BAC40515.1
AK143572 mRNA Translation: BAE25443.1
AK146181 mRNA Translation: BAE26959.1
AK150454 mRNA Translation: BAE29574.1
AK151052 mRNA Translation: BAE30069.1
AK151509 mRNA Translation: BAE30460.1
AK151868 mRNA Translation: BAE30756.1
AK152398 mRNA Translation: BAE31186.1
AK152848 mRNA Translation: BAE31541.1
AK168178 mRNA Translation: BAE40138.1
BC079903 mRNA Translation: AAH79903.1
BC147478 mRNA Translation: AAI47479.1
BC158035 mRNA Translation: AAI58036.1
BC171999 mRNA Translation: AAI71999.1
CCDSiCCDS38647.1
RefSeqiNP_058030.1, NM_016750.3
UniGeneiMm.117541
Mm.372513
Mm.465508

3D structure databases

ProteinModelPortaliP0C0S6
SMRiP0C0S6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206173, 5 interactors
IntActiP0C0S6, 13 interactors
MINTiP0C0S6
STRINGi10090.ENSMUSP00000036907

PTM databases

iPTMnetiP0C0S6
PhosphoSitePlusiP0C0S6

Proteomic databases

EPDiP0C0S6
MaxQBiP0C0S6
PaxDbiP0C0S6
PRIDEiP0C0S6
TopDownProteomicsiP0C0S6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041045; ENSMUSP00000036907; ENSMUSG00000037894
GeneIDi51788
KEGGimmu:51788
UCSCiuc008rmo.2 mouse

Organism-specific databases

CTDi3015
MGIiMGI:1888388 H2afz

Phylogenomic databases

eggNOGiKOG1757 Eukaryota
COG5262 LUCA
GeneTreeiENSGT00900000140979
HOGENOMiHOG000234652
HOVERGENiHBG009342
InParanoidiP0C0S6
KOiK11251
OMAiDKDKGKM
OrthoDBiEOG091G0XGD
PhylomeDBiP0C0S6
TreeFamiTF354232

Enzyme and pathway databases

ReactomeiR-MMU-171306 Packaging Of Telomere Ends
R-MMU-201722 Formation of the beta-catenin:TCF transactivating complex
R-MMU-212300 PRC2 methylates histones and DNA
R-MMU-2299718 Condensation of Prophase Chromosomes
R-MMU-2559580 Oxidative Stress Induced Senescence
R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-MMU-2559586 DNA Damage/Telomere Stress Induced Senescence
R-MMU-3214858 RMTs methylate histone arginines
R-MMU-427359 SIRT1 negatively regulates rRNA expression
R-MMU-427413 NoRC negatively regulates rRNA expression
R-MMU-5250924 B-WICH complex positively regulates rRNA expression
R-MMU-5578749 Transcriptional regulation by small RNAs
R-MMU-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3
R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-MMU-73728 RNA Polymerase I Promoter Opening
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

PROiPR:P0C0S6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037894 Expressed in 265 organ(s), highest expression level in primitive streak
CleanExiMM_H2AFZ
ExpressionAtlasiP0C0S6 baseline and differential
GenevisibleiP0C0S6 MM

Family and domain databases

CDDicd00074 H2A, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR002119 Histone_H2A
IPR007125 Histone_H2A/H2B/H3
IPR032454 Histone_H2A_C
IPR032458 Histone_H2A_CS
PfamiView protein in Pfam
PF00125 Histone, 1 hit
PF16211 Histone_H2A_C, 1 hit
PRINTSiPR00620 HISTONEH2A
SMARTiView protein in SMART
SM00414 H2A, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS00046 HISTONE_H2A, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiH2AZ_MOUSE
AccessioniPrimary (citable) accession number: P0C0S6
Secondary accession number(s): B2RXZ3
, P17317, Q3UA55, Q3UK43, Q68FD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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