UniProtKB - P0C0S1 (MSCS_ECOLI)
Small-conductance mechanosensitive channel
mscS
Functioni
Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell, opening in response to stretch forces in the membrane lipid bilayer, without the need for other proteins. Contributes to normal resistance to hypoosmotic shock. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage; as the membrane is depolarized, less tension is required to open the channel and vice versa. The channel is characterized by short bursts of activity that last for a few seconds.
11 PublicationsGO - Molecular functioni
- identical protein binding Source: IntAct
- mechanosensitive ion channel activity Source: UniProtKB
GO - Biological processi
- cellular water homeostasis Source: EcoCyc
- ion transmembrane transport Source: UniProtKB
- protein homooligomerization Source: UniProtKB
Keywordsi
Molecular function | Ion channel |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11160-MONOMER |
Protein family/group databases
TCDBi | 1.A.23.2.1, the small conductance mechanosensitive ion channel (mscs) family |
Names & Taxonomyi
Protein namesi | Recommended name: Small-conductance mechanosensitive channel |
Gene namesi | Name:mscS Synonyms:yggB Ordered Locus Names:b2924, JW2891 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 7 Publications; Multi-pass membrane protein 5 Publications
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 30 | Periplasmic1 PublicationAdd BLAST | 30 | |
Transmembranei | 31 – 52 | Helical2 PublicationsAdd BLAST | 22 | |
Topological domaini | 53 – 67 | Cytoplasmic1 PublicationAdd BLAST | 15 | |
Transmembranei | 68 – 88 | Helical2 PublicationsAdd BLAST | 21 | |
Topological domaini | 89 – 90 | Periplasmic1 Publication | 2 | |
Transmembranei | 91 – 111 | Helical2 PublicationsAdd BLAST | 21 | |
Topological domaini | 112 – 286 | Cytoplasmic1 PublicationAdd BLAST | 175 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 40 | V → C, G or N: No detectable phenotype. 1 Publication | 1 | |
Mutagenesisi | 40 | V → D or K: Normal growth stops, without cell death, due to increased membrane permeability to potassium ions and protons (permeability tested only for D substitutions). 1 Publication | 1 | |
Mutagenesisi | 58 | S → C: Readily forms disulfide bonds with cross-linkers, suggesting that individual S-58 are only 3 Angstroms apart in the closed state, versus 33 Angstroms apart in the open state crystal structure. 1 Publication | 1 | |
Mutagenesisi | 158 | A → F: Decreased conductance, due to decreased diameter of the channel portal. 1 Publication | 1 | |
Mutagenesisi | 266 – 286 | ISFPY…EDKAA → HHHHHHLE: Normal levels of channels are expressed; they recover more slowly than wild-type cells after desensitization. 1 PublicationAdd BLAST | 21 | |
Mutagenesisi | 266 – 286 | ISFPY…EDKAA → LE: Fewer channels present in the membrane, they require slightly more pressure to open and do not recover after desensitization. 1 PublicationAdd BLAST | 21 | |
Mutagenesisi | 267 | S → C: Provides biochemical evidence for heptameric structure upon cross-linking. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000110238 | 1 – 286 | Small-conductance mechanosensitive channelAdd BLAST | 286 |
Proteomic databases
jPOSTi | P0C0S1 |
PaxDbi | P0C0S1 |
PRIDEi | P0C0S1 |
Interactioni
Subunit structurei
Homoheptamer.
7 PublicationsBinary interactionsi
P0C0S1
With | #Exp. | IntAct |
---|---|---|
itself | 7 | EBI-554616,EBI-554616 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259238, 255 interactors |
DIPi | DIP-36192N |
IntActi | P0C0S1, 2 interactors |
STRINGi | 511145.b2924 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P0C0S1 |
SMRi | P0C0S1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0C0S1 |
Family & Domainsi
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0668, Bacteria |
HOGENOMi | CLU_037945_1_1_6 |
InParanoidi | P0C0S1 |
OMAi | GMQDTLT |
PhylomeDBi | P0C0S1 |
Family and domain databases
Gene3Di | 2.30.30.60, 1 hit |
InterProi | View protein in InterPro IPR010920, LSM_dom_sf IPR045275, MscS_archaea/bacteria_type IPR006685, MscS_channel IPR011066, MscS_channel_C IPR006686, MscS_channel_CS IPR011014, MscS_channel_TM-2 IPR023408, MscS_dom_sf IPR008910, TM_helix |
PANTHERi | PTHR30221, PTHR30221, 1 hit |
Pfami | View protein in Pfam PF00924, MS_channel, 1 hit PF05552, TM_helix, 1 hit |
SUPFAMi | SSF50182, SSF50182, 1 hit SSF82689, SSF82689, 1 hit SSF82861, SSF82861, 1 hit |
PROSITEi | View protein in PROSITE PS01246, UPF0003, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEDLNVVDSI NGAGSWLVAN QALLLSYAVN IVAALAIIIV GLIIARMISN
60 70 80 90 100
AVNRLMISRK IDATVADFLS ALVRYGIIAF TLIAALGRVG VQTASVIAVL
110 120 130 140 150
GAAGLAVGLA LQGSLSNLAA GVLLVMFRPF RAGEYVDLGG VAGTVLSVQI
160 170 180 190 200
FSTTMRTADG KIIVIPNGKI IAGNIINFSR EPVRRNEFII GVAYDSDIDQ
210 220 230 240 250
VKQILTNIIQ SEDRILKDRE MTVRLNELGA SSINFVVRVW SNSGDLQNVY
260 270 280
WDVLERIKRE FDAAGISFPY PQMDVNFKRV KEDKAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14436 Genomic DNA Translation: CAA32606.1 U28377 Genomic DNA Translation: AAA69091.1 U00096 Genomic DNA Translation: AAC75961.1 AP009048 Genomic DNA Translation: BAE76988.1 |
PIRi | S04735, QQEC4A |
RefSeqi | NP_417399.1, NC_000913.3 WP_000389818.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC75961; AAC75961; b2924 BAE76988; BAE76988; BAE76988 |
GeneIDi | 66673199 947416 |
KEGGi | ecj:JW2891 eco:b2924 |
PATRICi | fig|1411691.4.peg.3808 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14436 Genomic DNA Translation: CAA32606.1 U28377 Genomic DNA Translation: AAA69091.1 U00096 Genomic DNA Translation: AAC75961.1 AP009048 Genomic DNA Translation: BAE76988.1 |
PIRi | S04735, QQEC4A |
RefSeqi | NP_417399.1, NC_000913.3 WP_000389818.1, NZ_STEB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2OAU | X-ray | 3.70 | A/B/C/D/E/F/G | 1-286 | [»] | |
2VV5 | X-ray | 3.45 | A/B/C/D/E/F/G | 1-286 | [»] | |
3UDC | X-ray | 3.36 | A/B/C/D/E/F/G | 272-286 | [»] | |
4AGE | X-ray | 4.84 | A/B/C/D/E/F/G | 1-286 | [»] | |
4AGF | X-ray | 4.70 | A/B/C/D/E/F/G | 1-286 | [»] | |
4HWA | X-ray | 4.37 | A/B/C/D/E/F/G | 1-286 | [»] | |
5AJI | X-ray | 2.99 | A/B/C/D/E/F/G | 1-286 | [»] | |
6PWN | electron microscopy | 3.10 | A/B/C/D/E/F/G | 1-286 | [»] | |
6PWO | electron microscopy | 3.40 | A/B/C/D/E/F/G | 1-286 | [»] | |
6PWP | electron microscopy | 4.10 | A/B/C/D/E/F/G | 1-286 | [»] | |
6RLD | electron microscopy | 2.93 | A/B/C/D/E/F/G | 1-286 | [»] | |
6UZH | electron microscopy | 3.30 | A/B/C/D/E/F/G | 1-286 | [»] | |
6VYK | electron microscopy | 3.20 | A/B/C/D/E/F/G | 1-286 | [»] | |
6VYL | electron microscopy | 3.40 | A/B/C/D/E/F/G | 1-286 | [»] | |
6VYM | electron microscopy | 3.70 | A/B/C/D/E/F/G | 1-286 | [»] | |
7ONL | electron microscopy | 3.90 | A/B/C/D/E/F/G | 1-286 | [»] | |
AlphaFoldDBi | P0C0S1 | |||||
SMRi | P0C0S1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259238, 255 interactors |
DIPi | DIP-36192N |
IntActi | P0C0S1, 2 interactors |
STRINGi | 511145.b2924 |
Protein family/group databases
TCDBi | 1.A.23.2.1, the small conductance mechanosensitive ion channel (mscs) family |
Proteomic databases
jPOSTi | P0C0S1 |
PaxDbi | P0C0S1 |
PRIDEi | P0C0S1 |
Genome annotation databases
EnsemblBacteriai | AAC75961; AAC75961; b2924 BAE76988; BAE76988; BAE76988 |
GeneIDi | 66673199 947416 |
KEGGi | ecj:JW2891 eco:b2924 |
PATRICi | fig|1411691.4.peg.3808 |
Organism-specific databases
EchoBASEi | EB1149 |
Phylogenomic databases
eggNOGi | COG0668, Bacteria |
HOGENOMi | CLU_037945_1_1_6 |
InParanoidi | P0C0S1 |
OMAi | GMQDTLT |
PhylomeDBi | P0C0S1 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11160-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0C0S1 |
PROi | PR:P0C0S1 |
Family and domain databases
Gene3Di | 2.30.30.60, 1 hit |
InterProi | View protein in InterPro IPR010920, LSM_dom_sf IPR045275, MscS_archaea/bacteria_type IPR006685, MscS_channel IPR011066, MscS_channel_C IPR006686, MscS_channel_CS IPR011014, MscS_channel_TM-2 IPR023408, MscS_dom_sf IPR008910, TM_helix |
PANTHERi | PTHR30221, PTHR30221, 1 hit |
Pfami | View protein in Pfam PF00924, MS_channel, 1 hit PF05552, TM_helix, 1 hit |
SUPFAMi | SSF50182, SSF50182, 1 hit SSF82689, SSF82689, 1 hit SSF82861, SSF82861, 1 hit |
PROSITEi | View protein in PROSITE PS01246, UPF0003, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MSCS_ECOLI | |
Accessioni | P0C0S1Primary (citable) accession number: P0C0S1 Secondary accession number(s): P11666, Q2M9R8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | May 25, 2022 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families