UniProtKB - P0C0L7 (PROP_ECOLI)

BLAST

>sp|P0C0L7|PROP_ECOLI Proline/betaine transporter OS=Escherichia coli (strain K12) OX=83333 GN=proP PE=1 SV=1
MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGAD
PSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYD
TIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRKRGFMGSWLDFGSIAGFVLGA
GVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLRHALEETPAFQQHVDKLEQGDRE
GLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYYMLLTYMPSYLSHNLHYSEDHGVLI
IIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVALFVLAIPAFILINSNVIGLIFAGLLM
LAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNISVLVAGLTPTLAAWLVESSQNLMMP
AYYLMVVAVVGLITGVTMKETANRPLKGATPAASDIQEAKEILVEHYDNIEQKIDDIDHE
IADLQAKRTRLVQQHPRIDE

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History
Entry version 112 (07 Nov 2018)
Sequence version 1 (08 Nov 2005)
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Protein

Proline/betaine transporter

Gene

proP

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in the bacterial osmoregulatory response.3 Publications

Kineticsⁱ

K_M=
175 µM for proline
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Purification and reconstitution of an osmosensor: transporter ProP of Escherichia coli senses and responds to osmotic shifts."
  Racher K.I., Voegele R.T., Marshall E.V., Culham D.E., Wood J.M., Jung H., Bacon M., Cairns M.T., Ferguson S.M., Liang W.J., Henderson P.J., White G., Hallett F.R.
  Biochemistry 38:1676-1684(1999) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.

V_max=
83.6 nmol/min/mg enzyme with proline as substrate
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Purification and reconstitution of an osmosensor: transporter ProP of Escherichia coli senses and responds to osmotic shifts."
  Racher K.I., Voegele R.T., Marshall E.V., Culham D.E., Wood J.M., Jung H., Bacon M., Cairns M.T., Ferguson S.M., Liang W.J., Henderson P.J., White G., Hallett F.R.
  Biochemistry 38:1676-1684(1999) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.

GO - Molecular functionⁱ

glycine betaine:proton symporter activity
Source: EcoCyc
Inferred from direct assayⁱ
- 3049595

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

amino acid transport
Source: EcoliWiki
Inferred from direct assayⁱ
- 10026245
cellular hyperosmotic salinity response
Source: CAFA
Inferred from direct assayⁱ
- 15476391
osmosensory signaling pathway
Source: CAFA
Inferred from direct assayⁱ
- 15476391
proline import across plasma membrane
Source: CAFA
Inferred from direct assayⁱ
- 15476391

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Symport, Transport

Biological process

Symport, Transport

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PROP-MONOMER MetaCyc:PROP-MONOMER
SABIO-RKⁱ	P0C0L7

Protein family/group databases

TCDBⁱ	2.A.1.6.4 the major facilitator superfamily (mfs)

TCDBⁱ

2.A.1.6.4 the major facilitator superfamily (mfs)

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Proline/betaine transporter Alternative name(s): Proline porter II Short name: PPII
Gene namesⁱ	Name:proP Ordered Locus Names:b4111, JW4072
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11612 proP

EcoGeneⁱ

EG11612 proP

Subcellular locationⁱ

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 37	CytoplasmicSequence analysisAdd BLAST	37
Transmembraneⁱ	38 – 58	Helical; Name=1Sequence analysisAdd BLAST	21
Topological domainⁱ	59 – 65	PeriplasmicSequence analysis	7
Transmembraneⁱ	66 – 86	Helical; Name=2Sequence analysisAdd BLAST	21
Topological domainⁱ	87 – 97	CytoplasmicSequence analysisAdd BLAST	11
Transmembraneⁱ	98 – 118	Helical; Name=3Sequence analysisAdd BLAST	21
Topological domainⁱ	119 – 121	PeriplasmicSequence analysis	3
Transmembraneⁱ	122 – 142	Helical; Name=4Sequence analysisAdd BLAST	21
Topological domainⁱ	143 – 169	CytoplasmicSequence analysisAdd BLAST	27
Transmembraneⁱ	170 – 190	Helical; Name=5Sequence analysisAdd BLAST	21
Topological domainⁱ	191 – 194	PeriplasmicSequence analysis	4
Transmembraneⁱ	195 – 215	Helical; Name=6Sequence analysisAdd BLAST	21
Topological domainⁱ	216 – 260	CytoplasmicSequence analysisAdd BLAST	45
Transmembraneⁱ	261 – 281	Helical; Name=7Sequence analysisAdd BLAST	21
Topological domainⁱ	282 – 297	PeriplasmicSequence analysisAdd BLAST	16
Transmembraneⁱ	298 – 318	Helical; Name=8Sequence analysisAdd BLAST	21
Topological domainⁱ	319 – 325	CytoplasmicSequence analysis	7
Transmembraneⁱ	326 – 346	Helical; Name=9Sequence analysisAdd BLAST	21
Topological domainⁱ	347 – 350	PeriplasmicSequence analysis	4
Transmembraneⁱ	351 – 371	Helical; Name=10Sequence analysisAdd BLAST	21
Topological domainⁱ	372 – 390	CytoplasmicSequence analysisAdd BLAST	19
Transmembraneⁱ	391 – 411	Helical; Name=11Sequence analysisAdd BLAST	21
Topological domainⁱ	412 – 416	PeriplasmicSequence analysis	5
Transmembraneⁱ	417 – 437	Helical; Name=12Sequence analysisAdd BLAST	21
Topological domainⁱ	438 – 500	Cytoplasmic1 PublicationAdd BLAST	63

GO - Cellular componentⁱ

integral component of membrane
Source: EcoliWiki
Inferred from direct assayⁱ
- 10026245
integral component of plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 10026245
plasma membrane
Source: CAFA
Inferred from direct assayⁱ
- 15476391

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000050324	1 – 500	Proline/betaine transporterAdd BLAST		500

Proteomic databases

PaxDbⁱ	P0C0L7
PRIDEⁱ	P0C0L7

Expressionⁱ

Inductionⁱ

Induced by an increase in the osmolarity of the growth medium.1 Publication

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4263085, 22 interactors
STRINGⁱ	316385.ECDH10B_4303

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Helixⁱ	469 – 494	Combined sources		26

Show more details

3D structure databases

ProteinModelPortalⁱ	P0C0L7
SMRⁱ	P0C0L7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P0C0L7

Family & Domainsⁱ

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	453 – 498	Sequence analysisAdd BLAST		46

Sequence similaritiesⁱ

Belongs to the major facilitator superfamily. Metabolite:H+ Symporter (MHS) family (TC 2.A.1.6) family. [View classification]Curated

Keywords - Domainⁱ

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4105CSH Bacteria ENOG410XP7I LUCA
HOGENOMⁱ	HOG000141611
InParanoidⁱ	P0C0L7
KEGG Orthology (KO) More...KOⁱ	K03762
PhylomeDBⁱ	P0C0L7

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06174 MFS, 1 hit
InterProⁱ	View protein in InterPro IPR020846 MFS_dom IPR005828 MFS_sugar_transport-like IPR036259 MFS_trans_sf IPR004736 MHS_symport IPR015041 Osmo_CC IPR036292 ProP_C IPR005829 Sugar_transporter_CS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08946 Osmo_CC, 1 hit PF00083 Sugar_tr, 1 hit
SUPFAMⁱ	SSF103473 SSF103473, 1 hit SSF103661 SSF103661, 1 hit
TIGRFAMsⁱ	TIGR00883 2A0106, 1 hit
PROSITEⁱ	View protein in PROSITE PS50850 MFS, 1 hit PS00216 SUGAR_TRANSPORT_1, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0C0L7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA 
        60         70         80         90        100
LGKVFFPGAD PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA 
       110        120        130        140        150
ITIVIMSIST FCIGLIPSYD TIGIWAPILL LICKMAQGFS VGGEYTGASI 
       160        170        180        190        200
FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA GVVVLISTIV GEANFLDWGW 
       210        220        230        240        250
RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE GLQDGPKVSF 
       260        270        280        290        300
KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI 
       310        320        330        340        350
IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV 
       360        370        380        390        400
IGLIFAGLLM LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA 
       410        420        430        440        450
GLTPTLAAWL VESSQNLMMP AYYLMVVAVV GLITGVTMKE TANRPLKGAT 
       460        470        480        490        500
PAASDIQEAK EILVEHYDNI EQKIDDIDHE IADLQAKRTR LVQQHPRIDE

Length:500

Mass (Da):54,846

Last modified:November 8, 2005 - v1

Checksum:ⁱ60EDDBF61190DDFC

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M83089 Genomic DNA Translation: AAB00919.1 U14003 Genomic DNA Translation: AAA97010.1 U00096 Genomic DNA Translation: AAC77072.1 AP009048 Genomic DNA Translation: BAE78113.1
PIRⁱ	S32331
NCBI Reference Sequences More...RefSeqⁱ	NP_418535.1, NC_000913.3 WP_001298520.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC77072; AAC77072; b4111 BAE78113; BAE78113; BAE78113
GeneIDⁱ	948626
KEGGⁱ	ecj:JW4072 eco:b4111
PATRICⁱ	fig\|1411691.4.peg.2589

Protein	Similar proteins		Species	Score	Length	Source
P0C0L7	Proline/betaine transporter		ECO57		500	UniRef100_P0C0L8
Proline/betaine transporter		Shigella dysenteriae WRSd3		511
Proline/betaine transporter		Shigella dysenteriae 1617		511
Proline/betaine transporter (Fragment)		ECOLX		409
Proline/betaine transporter		Shigella sp.		500
+52

Protein	Similar proteins		Species	Score	Length	Source
P0C0L7	Proline/betaine transporter		SALTY		500	UniRef90_P40862
Proline/betaine transporter		ECO57		500
Proline porter II		Salmonella enterica subsp. enterica serovar Enteritidis str. 2009K0958		504
Proline porter II		Salmonella enterica subsp. enterica serovar Dublin str. UC16		504
Proline porter II		Salmonella enterica subsp. enterica serovar Cubana str. 76814		504
+924

Protein	Similar proteins		Species	Score	Length	Source
P0C0L7	Glycine betaine/proline/ectoine/pipecolic acid transporter OusA		DICD3		501	UniRef50_Q47421
Proline/betaine transporter		ECO57		500
Proline/betaine transporter		SALTY		500
Metabolite/H+ symporter, major facilitator superfamily (MFS)		DICZ5		501
Metabolite/H+ symporter, major facilitator superfamily (MFS)		DICC1		501
+2339

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M83089 Genomic DNA Translation: AAB00919.1 U14003 Genomic DNA Translation: AAA97010.1 U00096 Genomic DNA Translation: AAC77072.1 AP009048 Genomic DNA Translation: BAE78113.1
PIRⁱ	S32331
RefSeqⁱ	NP_418535.1, NC_000913.3 WP_001298520.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1R48	NMR	-	A/B	468-497	[»]
	1Y8S	model	-	A	1-500	[»]
ProteinModelPortalⁱ	P0C0L7
SMRⁱ	P0C0L7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263085, 22 interactors
STRINGⁱ	316385.ECDH10B_4303

Protein family/group databases

TCDBⁱ	2.A.1.6.4 the major facilitator superfamily (mfs)

TCDBⁱ

2.A.1.6.4 the major facilitator superfamily (mfs)

Proteomic databases

PaxDbⁱ	P0C0L7
PRIDEⁱ	P0C0L7

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC77072; AAC77072; b4111 BAE78113; BAE78113; BAE78113
GeneIDⁱ	948626
KEGGⁱ	ecj:JW4072 eco:b4111
PATRICⁱ	fig\|1411691.4.peg.2589

Organism-specific databases

EchoBASEⁱ	EB1569
EcoGeneⁱ	EG11612 proP

Phylogenomic databases

eggNOGⁱ	ENOG4105CSH Bacteria ENOG410XP7I LUCA
HOGENOMⁱ	HOG000141611
InParanoidⁱ	P0C0L7
KOⁱ	K03762
PhylomeDBⁱ	P0C0L7

Enzyme and pathway databases

BioCycⁱ	EcoCyc:PROP-MONOMER MetaCyc:PROP-MONOMER
SABIO-RKⁱ	P0C0L7

Miscellaneous databases

EvolutionaryTraceⁱ	P0C0L7
Protein Ontology More...PROⁱ	PR:P0C0L7

Family and domain databases

CDDⁱ	cd06174 MFS, 1 hit
InterProⁱ	View protein in InterPro IPR020846 MFS_dom IPR005828 MFS_sugar_transport-like IPR036259 MFS_trans_sf IPR004736 MHS_symport IPR015041 Osmo_CC IPR036292 ProP_C IPR005829 Sugar_transporter_CS
Pfamⁱ	View protein in Pfam PF08946 Osmo_CC, 1 hit PF00083 Sugar_tr, 1 hit
SUPFAMⁱ	SSF103473 SSF103473, 1 hit SSF103661 SSF103661, 1 hit
TIGRFAMsⁱ	TIGR00883 2A0106, 1 hit
PROSITEⁱ	View protein in PROSITE PS50850 MFS, 1 hit PS00216 SUGAR_TRANSPORT_1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PROP_ECOLI
Accessionⁱ	P0C0L7Primary (citable) accession number: P0C0L7 Secondary accession number(s): P30848, Q2M6J3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
	Last sequence update:	November 8, 2005
	Last modified:	November 7, 2018
	This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0C0L7 (PROP_ECOLI)

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Proline/betaine transporter

proP

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Kineticsⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ