UniProtKB - P0C0L7 (PROP_ECOLI)
Protein
Proline/betaine transporter
Gene
proP
Organism
Escherichia coli (strain K12)
Status
Functioni
Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in the bacterial osmoregulatory response.3 Publications
Kineticsi
- KM=175 µM for proline1 Publication
- Vmax=83.6 nmol/min/mg enzyme with proline as substrate1 Publication
GO - Molecular functioni
- proline:proton symporter activity Source: EcoCyc
GO - Biological processi
- amino acid transport Source: EcoliWiki
- cellular hyperosmotic response Source: EcoCyc
- cellular hyperosmotic salinity response Source: CAFA
- glycine betaine transport Source: EcoCyc
- osmosensory signaling pathway Source: CAFA
- proline import across plasma membrane Source: CAFA
Keywordsi
Biological process | Symport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:PROP-MONOMER MetaCyc:PROP-MONOMER |
SABIO-RKi | P0C0L7 |
Protein family/group databases
TCDBi | 2.A.1.6.4, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Proline/betaine transporterAlternative name(s): Proline porter II Short name: PPII |
Gene namesi | Name:proP Ordered Locus Names:b4111, JW4072 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: CAFA
Other locations
- integral component of membrane Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 37 | CytoplasmicSequence analysisAdd BLAST | 37 | |
Transmembranei | 38 – 58 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 59 – 65 | PeriplasmicSequence analysis | 7 | |
Transmembranei | 66 – 86 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 87 – 97 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 98 – 118 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 119 – 121 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 122 – 142 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 143 – 169 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 170 – 190 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 191 – 194 | PeriplasmicSequence analysis | 4 | |
Transmembranei | 195 – 215 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 216 – 260 | CytoplasmicSequence analysisAdd BLAST | 45 | |
Transmembranei | 261 – 281 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 282 – 297 | PeriplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 298 – 318 | Helical; Name=8Sequence analysisAdd BLAST | 21 | |
Topological domaini | 319 – 325 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 326 – 346 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 347 – 350 | PeriplasmicSequence analysis | 4 | |
Transmembranei | 351 – 371 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 372 – 390 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 391 – 411 | Helical; Name=11Sequence analysisAdd BLAST | 21 | |
Topological domaini | 412 – 416 | PeriplasmicSequence analysis | 5 | |
Transmembranei | 417 – 437 | Helical; Name=12Sequence analysisAdd BLAST | 21 | |
Topological domaini | 438 – 500 | Cytoplasmic1 PublicationAdd BLAST | 63 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050324 | 1 – 500 | Proline/betaine transporterAdd BLAST | 500 |
Proteomic databases
jPOSTi | P0C0L7 |
PaxDbi | P0C0L7 |
PRIDEi | P0C0L7 |
Expressioni
Inductioni
Induced by an increase in the osmolarity of the growth medium.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263085, 22 interactors |
STRINGi | 511145.b4111 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0C0L7 |
SMRi | P0C0L7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0C0L7 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 453 – 498 | Sequence analysisAdd BLAST | 46 |
Sequence similaritiesi
Belongs to the major facilitator superfamily. Metabolite:H+ Symporter (MHS) family (TC 2.A.1.6) family. [View classification]Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0477, Bacteria |
HOGENOMi | CLU_001265_39_0_6 |
InParanoidi | P0C0L7 |
PhylomeDBi | P0C0L7 |
Family and domain databases
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR004736, MHS_symport IPR015041, Osmo_CC IPR036292, ProP_C IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF08946, Osmo_CC, 1 hit PF00083, Sugar_tr, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit SSF103661, SSF103661, 1 hit |
TIGRFAMsi | TIGR00883, 2A0106, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P0C0L7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA
60 70 80 90 100
LGKVFFPGAD PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA
110 120 130 140 150
ITIVIMSIST FCIGLIPSYD TIGIWAPILL LICKMAQGFS VGGEYTGASI
160 170 180 190 200
FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA GVVVLISTIV GEANFLDWGW
210 220 230 240 250
RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE GLQDGPKVSF
260 270 280 290 300
KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI
310 320 330 340 350
IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV
360 370 380 390 400
IGLIFAGLLM LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA
410 420 430 440 450
GLTPTLAAWL VESSQNLMMP AYYLMVVAVV GLITGVTMKE TANRPLKGAT
460 470 480 490 500
PAASDIQEAK EILVEHYDNI EQKIDDIDHE IADLQAKRTR LVQQHPRIDE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83089 Genomic DNA Translation: AAB00919.1 U14003 Genomic DNA Translation: AAA97010.1 U00096 Genomic DNA Translation: AAC77072.1 AP009048 Genomic DNA Translation: BAE78113.1 |
PIRi | S32331 |
RefSeqi | NP_418535.1, NC_000913.3 WP_001298520.1, NZ_SSZK01000018.1 |
Genome annotation databases
EnsemblBacteriai | AAC77072; AAC77072; b4111 BAE78113; BAE78113; BAE78113 |
GeneIDi | 948626 |
KEGGi | ecj:JW4072 eco:b4111 |
PATRICi | fig|1411691.4.peg.2589 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83089 Genomic DNA Translation: AAB00919.1 U14003 Genomic DNA Translation: AAA97010.1 U00096 Genomic DNA Translation: AAC77072.1 AP009048 Genomic DNA Translation: BAE78113.1 |
PIRi | S32331 |
RefSeqi | NP_418535.1, NC_000913.3 WP_001298520.1, NZ_SSZK01000018.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R48 | NMR | - | A/B | 468-497 | [»] | |
1Y8S | model | - | A | 1-500 | [»] | |
BMRBi | P0C0L7 | |||||
SMRi | P0C0L7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263085, 22 interactors |
STRINGi | 511145.b4111 |
Protein family/group databases
TCDBi | 2.A.1.6.4, the major facilitator superfamily (mfs) |
Proteomic databases
jPOSTi | P0C0L7 |
PaxDbi | P0C0L7 |
PRIDEi | P0C0L7 |
Genome annotation databases
EnsemblBacteriai | AAC77072; AAC77072; b4111 BAE78113; BAE78113; BAE78113 |
GeneIDi | 948626 |
KEGGi | ecj:JW4072 eco:b4111 |
PATRICi | fig|1411691.4.peg.2589 |
Organism-specific databases
EchoBASEi | EB1569 |
Phylogenomic databases
eggNOGi | COG0477, Bacteria |
HOGENOMi | CLU_001265_39_0_6 |
InParanoidi | P0C0L7 |
PhylomeDBi | P0C0L7 |
Enzyme and pathway databases
BioCyci | EcoCyc:PROP-MONOMER MetaCyc:PROP-MONOMER |
SABIO-RKi | P0C0L7 |
Miscellaneous databases
EvolutionaryTracei | P0C0L7 |
PROi | PR:P0C0L7 |
Family and domain databases
InterProi | View protein in InterPro IPR020846, MFS_dom IPR005828, MFS_sugar_transport-like IPR036259, MFS_trans_sf IPR004736, MHS_symport IPR015041, Osmo_CC IPR036292, ProP_C IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF08946, Osmo_CC, 1 hit PF00083, Sugar_tr, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit SSF103661, SSF103661, 1 hit |
TIGRFAMsi | TIGR00883, 2A0106, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS00216, SUGAR_TRANSPORT_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PROP_ECOLI | |
Accessioni | P0C0L7Primary (citable) accession number: P0C0L7 Secondary accession number(s): P30848, Q2M6J3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | November 8, 2005 | |
Last modified: | April 7, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families