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UniProtKB - P0C0K3 (SRKA_ECOLI)
Protein
Stress response kinase A
Gene
srkA
Organism
Escherichia coli (strain K12)
Status
Functioni
A protein kinase that (auto)phosphorylates on Ser and Thr residues (PubMed:17302814).
Probably acts to suppress the effects of stress linked to accumulation of reactive oxygen species. Protects cells from stress by antagonizing the MazE-MazF TA module, probably indirectly as it has not been seen to phosphorylate MazE, MazF or MazG (PubMed:23416055).
Probably involved in the extracytoplasmic stress response (PubMed:9159398).
UniRule annotation3 PublicationsCatalytic activityi
- EC:2.7.11.1UniRule annotation1 Publication
- ATP + L-threonyl-[protein] = ADP + H+ + O-phospho-L-threonyl-[protein]UniRule annotation1 PublicationEC:2.7.11.1UniRule annotation1 Publication
Cofactori
Mg2+1 PublicationNote: May bind 2 Mg2+ ions.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 36 | ATPUniRule annotation1 Publication | 1 | |
Active sitei | 201 | Proton acceptorUniRule annotation1 Publication | 1 | |
Metal bindingi | 206 | MagnesiumUniRule annotation1 Publication | 1 | |
Active sitei | 217 | UniRule annotation1 Publication | 1 | |
Metal bindingi | 217 | MagnesiumUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: EcoCyc
- protein serine kinase activity Source: RHEA
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Stress response |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11831-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Stress response kinase A1 PublicationUniRule annotation (EC:2.7.11.1UniRule annotation1 Publication)Alternative name(s): Serine/threonine protein kinase YihE1 Publication Serine/threonine-protein kinase SrkAUniRule annotationCurated |
Gene namesi | Name:srkA1 PublicationUniRule annotation Synonyms:rdoA, yihE Ordered Locus Names:b3859, JW3831 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Other locations
- cytoplasm Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
A hyperlethal phenotype (reduced survival in the presence of antibiotic but no change in minimal inhibitory concentration) for a number of antimicrobials including nalidixic acid, tetracycline, ampicillin and mitomycin C as well as exposure to UV light and H2O2. Lethality is mitigated by pretreatment with 2,2'-bipyridyl and thiourea, which inhibit hydroxyl radical accumulation. No change in cell survival upon heat shock (up to 55 degrees Celsius), rifampicin or fluoroquinolone PD161144 treatment. A triple srkA-mazE-mazF disruption mutant shows no hyperlethality in the presence of nalidixic acid or UV light, suggesting SrkA has a negative effect on MazF. Double katG-srkA and double cpxR-srkA disruption mutants are as sensitive to killing as single srkA mutants; i.e. all 3 genes are epistatic and function in the same genetic pathway.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 36 | S → A: Partial loss of kinase activity, only partially protects bacteria against hyperlethal stress. 1 Publication | 1 | |
Mutagenesisi | 217 | D → A: Loss of kinase activity; does not protect bacteria against hyperlethal stress. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209574 | 1 – 328 | Stress response kinase AAdd BLAST | 328 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0C0K3 |
PaxDbi | P0C0K3 |
PRIDEi | P0C0K3 |
Expressioni
Inductioni
Induced by the two-component Cpx system that responds to extracellular stress.1 Publication
Interactioni
Subunit structurei
Monomer.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4261338, 3 interactors |
IntActi | P0C0K3, 5 interactors |
STRINGi | 511145.b3859 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0C0K3 |
SMRi | P0C0K3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0C0K3 |
Family & Domainsi
Sequence similaritiesi
Belongs to the SrkA/RdoA protein kinase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG2334, Bacteria |
HOGENOMi | CLU_054715_0_0_6 |
InParanoidi | P0C0K3 |
OMAi | MHYSAWL |
PhylomeDBi | P0C0K3 |
Family and domain databases
HAMAPi | MF_01497, SrkA_kinase, 1 hit |
InterProi | View protein in InterPro IPR002575, Aminoglycoside_PTrfase IPR011009, Kinase-like_dom_sf IPR032882, SrkA/RdoA |
PANTHERi | PTHR39573, PTHR39573, 1 hit |
Pfami | View protein in Pfam PF01636, APH, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
i Sequence
Sequence statusi: Complete.
P0C0K3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNNSAFTFQT LHPDTIMDAL FEHGIRVDSG LTPLNSYENR VYQFQDEDRR
60 70 80 90 100
RFVVKFYRPE RWTADQILEE HQFALQLVND EVPVAAPVAF NGQTLLNHQG
110 120 130 140 150
FYFAVFPSVG GRQFEADNID QMEAVGRYLG RMHQTGRKQL FIHRPTIGLN
160 170 180 190 200
EYLIEPRKLF EDATLIPSGL KAAFLKATDE LIAAVTAHWR EDFTVLRLHG
210 220 230 240 250
DCHAGNILWR DGPMFVDLDD ARNGPAVQDL WMLLNGDKAE QRMQLETIIE
260 270 280 290 300
AYEEFSEFDT AEIGLIEPLR AMRLVYYLAW LMRRWADPAF PKNFPWLTGE
310 320
DYWLRQTATF IEQAKVLQEP PLQLTPMY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80762 Genomic DNA Translation: CAA56735.1 L19201 Genomic DNA Translation: AAB02994.1 U00096 Genomic DNA Translation: AAC76857.1 AP009048 Genomic DNA Translation: BAE77449.1 |
PIRi | S40805 |
RefSeqi | NP_418296.1, NC_000913.3 WP_001065497.1, NZ_SSZK01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC76857; AAC76857; b3859 BAE77449; BAE77449; BAE77449 |
GeneIDi | 948346 |
KEGGi | ecj:JW3831 eco:b3859 |
PATRICi | fig|511145.12.peg.3968 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80762 Genomic DNA Translation: CAA56735.1 L19201 Genomic DNA Translation: AAB02994.1 U00096 Genomic DNA Translation: AAC76857.1 AP009048 Genomic DNA Translation: BAE77449.1 |
PIRi | S40805 |
RefSeqi | NP_418296.1, NC_000913.3 WP_001065497.1, NZ_SSZK01000026.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZYL | X-ray | 2.80 | A | 1-328 | [»] | |
AlphaFoldDBi | P0C0K3 | |||||
SMRi | P0C0K3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261338, 3 interactors |
IntActi | P0C0K3, 5 interactors |
STRINGi | 511145.b3859 |
Proteomic databases
jPOSTi | P0C0K3 |
PaxDbi | P0C0K3 |
PRIDEi | P0C0K3 |
Genome annotation databases
EnsemblBacteriai | AAC76857; AAC76857; b3859 BAE77449; BAE77449; BAE77449 |
GeneIDi | 948346 |
KEGGi | ecj:JW3831 eco:b3859 |
PATRICi | fig|511145.12.peg.3968 |
Organism-specific databases
EchoBASEi | EB1778 |
Phylogenomic databases
eggNOGi | COG2334, Bacteria |
HOGENOMi | CLU_054715_0_0_6 |
InParanoidi | P0C0K3 |
OMAi | MHYSAWL |
PhylomeDBi | P0C0K3 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11831-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0C0K3 |
PROi | PR:P0C0K3 |
Family and domain databases
HAMAPi | MF_01497, SrkA_kinase, 1 hit |
InterProi | View protein in InterPro IPR002575, Aminoglycoside_PTrfase IPR011009, Kinase-like_dom_sf IPR032882, SrkA/RdoA |
PANTHERi | PTHR39573, PTHR39573, 1 hit |
Pfami | View protein in Pfam PF01636, APH, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SRKA_ECOLI | |
Accessioni | P0C0K3Primary (citable) accession number: P0C0K3 Secondary accession number(s): P32127, Q2M8F7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2005 |
Last sequence update: | September 27, 2005 | |
Last modified: | May 25, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families