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Protein

Nucleoid occlusion factor SlmA

Gene

slmA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.UniRule annotation3 Publications

Miscellaneous

Inhibitory activity is enhanced by sequence-specific DNA binding.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi33 – 52H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • DNA binding transcription factor activity Source: GO_Central
  • identical protein binding Source: IntAct
  • sequence-specific DNA binding Source: EcoCyc
  • transcription regulatory region sequence-specific DNA binding Source: GO_Central

GO - Biological processi

  • division septum site selection Source: EcoliWiki
  • negative regulation of division septum assembly Source: EcoliWiki
  • negative regulation of protein polymerization Source: EcoCyc
  • positive regulation of GTPase activity Source: EcoliWiki

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG11191-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoid occlusion factor SlmAUniRule annotation
Alternative name(s):
Protein Ttk
Synthetically lethal with a defective Min system protein A
Gene namesi
Name:slmAUniRule annotation
Synonyms:ttk, yicB
Ordered Locus Names:b3641, JW5641
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11191 slmA

Subcellular locationi

  • nucleoid UniRule annotation1 Publication

GO - Cellular componenti

  • bacterial nucleoid Source: EcoCyc
  • cytosol Source: GO_Central

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33T → A: Does not associate with DNA, but can inhibit division when overproduced. 1 Publication1
Mutagenesisi73R → D: Loss of activity. Binds DNA, but does not associate with FtsZ polymers. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001989661 – 198Nucleoid occlusion factor SlmAAdd BLAST198

Proteomic databases

PaxDbiP0C093
PRIDEiP0C093

Interactioni

Subunit structurei

Homodimer. Interacts with FtsZ.UniRule annotation3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8520580,EBI-8520580

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259414, 342 interactors
IntActiP0C093, 1 interactor
MINTiP0C093
STRINGi316385.ECDH10B_3823

Structurei

Secondary structure

1198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 24Combined sources13
Helixi26 – 29Combined sources4
Helixi34 – 40Combined sources7
Helixi45 – 49Combined sources5
Helixi55 – 80Combined sources26
Helixi84 – 101Combined sources18
Helixi103 – 109Combined sources7
Beta strandi115 – 117Combined sources3
Helixi122 – 141Combined sources20
Turni142 – 146Combined sources5
Helixi155 – 174Combined sources20
Turni175 – 178Combined sources4
Turni182 – 185Combined sources4
Helixi186 – 194Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NXCX-ray2.50A1-198[»]
5HBUX-ray2.60A/B/C/D/E/F/G/H7-198[»]
5K58X-ray2.77A/B/E/F9-198[»]
ProteinModelPortaliP0C093
SMRiP0C093
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 70HTH tetR-typeUniRule annotationAdd BLAST61

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili117 – 144UniRule annotationAdd BLAST28

Sequence similaritiesi

Belongs to the nucleoid occlusion factor SlmA family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4108ENE Bacteria
ENOG410XQXX LUCA
HOGENOMiHOG000266053
InParanoidiP0C093
KOiK05501
OMAiIEQTVFG
PhylomeDBiP0C093

Family and domain databases

HAMAPiMF_01839 NO_factor_SlmA, 1 hit
InterProiView protein in InterPro
IPR023772 DNA-bd_HTH_TetR-type_CS
IPR009057 Homeobox-like_sf
IPR001647 HTH_TetR
IPR023769 NO_SlmA
IPR036271 Tet_transcr_reg_TetR-rel_C_sf
PfamiView protein in Pfam
PF00440 TetR_N, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF48498 SSF48498, 1 hit
PROSITEiView protein in PROSITE
PS01081 HTH_TETR_1, 1 hit
PS50977 HTH_TETR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0C093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKQTAKRN RREEILQSLA LMLESSDGSQ RITTAKLAAS VGVSEAALYR
60 70 80 90 100
HFPSKTRMFD SLIEFIEDSL ITRINLILKD EKDTTARLRL IVLLLLGFGE
110 120 130 140 150
RNPGLTRILT GHALMFEQDR LQGRINQLFE RIEAQLRQVL REKRMREGEG
160 170 180 190
YTTDETLLAS QILAFCEGML SRFVRSEFKY RPTDDFDARW PLIAAQLQ
Length:198
Mass (Da):22,836
Last modified:August 16, 2005 - v1
Checksum:iC797B0E2875B0E39
GO

Sequence cautioni

The sequence AAA61994 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196 – 198QLQ → SCSNMTPDDFSSGEFL in CAA25860 (PubMed:6139280).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01714 Genomic DNA Translation: CAA25860.1
V01578 Genomic DNA Translation: CAA24898.1
V01498 Genomic DNA No translation available.
L10328 Genomic DNA Translation: AAA61994.1 Different initiation.
U00096 Genomic DNA Translation: AAC76665.2
AP009048 Genomic DNA Translation: BAE77651.1
RefSeqiNP_418098.4, NC_000913.3
WP_000818601.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76665; AAC76665; b3641
BAE77651; BAE77651; BAE77651
GeneIDi948158
KEGGiecj:JW5641
eco:b3641
PATRICifig|1411691.4.peg.3065

Similar proteinsi

Entry informationi

Entry nameiSLMA_ECOLI
AccessioniPrimary (citable) accession number: P0C093
Secondary accession number(s): P06969, Q2M7V5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: August 16, 2005
Last modified: March 28, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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