Membrane-bound lytic murein transglycosylase C

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityⁱ

• Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

  Manual assertion according to rulesⁱ

  • HAMAP-Rule:MF_01616

  EC:4.2.2.n1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• hydrolase activity, acting on glycosyl bonds Source: InterPro
• lyase activity Source: UniProtKB-KW
• lytic endotransglycosylase activity Source: EcoCyc <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Reactions of all Escherichia coli lytic transglycosylases with bacterial cell wall."
    Lee M., Hesek D., Llarrull L.I., Lastochkin E., Pi H., Boggess B., Mobashery S.
    J. Am. Chem. Soc. 135:3311-3314(2013) [PubMed] [Europe PMC] [Abstract]
• lytic transglycosylase activity Source: EcoCycInferred from direct assayⁱ
  • "Reactions of all Escherichia coli lytic transglycosylases with bacterial cell wall."
    Lee M., Hesek D., Llarrull L.I., Lastochkin E., Pi H., Boggess B., Mobashery S.
    J. Am. Chem. Soc. 135:3311-3314(2013) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• cellular response to antibiotic Source: EcoCyc <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Susceptibility to antibiotics and beta-lactamase induction in murein hydrolase mutants of Escherichia coli."
    Korsak D., Liebscher S., Vollmer W.
    Antimicrob. Agents Chemother. 49:1404-1409(2005) [PubMed] [Europe PMC] [Abstract]
• cellular response to oxidative stress Source: EcoCyc <p>Inferred from Expression Pattern</p> <p>Covers cases where the annotation is inferred from the timing or location of expression of a gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iep">GO evidence code guide</a></p> Inferred from expression patternⁱ
  • Ref.4

    "SoxRS-regulated expression and genetic analysis of the yggX gene of Escherichia coli."
    Pomposiello P.J., Koutsolioutsou A., Carrasco D., Demple B.
    J. Bacteriol. 185:6624-6632(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: INDUCTION.
• cell wall macromolecule catabolic process Source: UniProtKB-UniRule
• cell wall organization Source: UniProtKB-KW
• peptidoglycan catabolic process Source: EcoCycInferred from direct assayⁱ
  • Ref.3

    "Identification of new members of the lytic transglycosylase family in Haemophilus influenzae and Escherichia coli."
    Dijkstra A.J., Keck W.
    Microb. Drug Resist. 2:141-145(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 162-200, IDENTIFICATION.

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

GO - Cellular componentⁱ

• anchored component of membrane Source: EcoCycInferred from sequence modelⁱ
  • Ref.3

    "Identification of new members of the lytic transglycosylase family in Haemophilus influenzae and Escherichia coli."
    Dijkstra A.J., Keck W.
    Microb. Drug Resist. 2:141-145(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 162-200, IDENTIFICATION.
• cell outer membrane Source: UniProtKB-SubCell
• outer membrane-bounded periplasmic space Source: EcoCyc <p>Traceable Author Statement</p> <p>Used for information from review articles where the original experiments are traceable through that article and also for information from text books or dictionaries.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#tas">GO evidence code guide</a></p> Traceable author statementⁱ
  • "Fine-tuning the prediction of sequences cleaved by signal peptidase II: a curated set of proven and predicted lipoproteins of Escherichia coli K-12."
    Gonnet P., Rudd K.E., Lisacek F.
    Proteomics 4:1597-1613(2004) [PubMed] [Europe PMC] [Abstract]

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductionⁱ

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingⁱ: The displayed sequence is further processed into a mature form.

        10         20         30         40         50
MKKYLALALI APLLISCSTT KKGDTYNEAW VKDTNGFDIL MGQFAHNIEN 
        60         70         80         90        100
IWGFKEVVIA GPKDYVKYTD QYQTRSHINF DDGTITIETI AGTEPAAHLR 
       110        120        130        140        150
RAIIKTLLMG DDPSSVDLYS DVDDITISKE PFLYGQVVDN TGQPIRWEGR 
       160        170        180        190        200
ASNFADYLLK NRLKSRSNGL RIIYSVTINM VPNHLDKRAH KYLGMVRQAS 
       210        220        230        240        250
RKYGVDESLI LAIMQTESSF NPYAVSRSDA LGLMQVVQHT AGKDVFRSQG 
       260        270        280        290        300
KSGTPSRSFL FDPASNIDTG TAYLAMLNNV YLGGIDNPTS RRYAVITAYN 
       310        320        330        340        350
GGAGSVLRVF SNDKIQAANI INTMTPGDVY QTLTTRHPSA ESRRYLYKVN 

TAQKSYRRR                                              

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautionⁱ

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
2. SIMILARITY comments
   Index of protein domains and families
3. Escherichia coli
   Escherichia coli (strain K12): entries and cross-references to EcoGene