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UniProtKB - P0C062 (GRSA_BREBE)
Protein
Gramicidin S synthase 1
Gene
grsA
Organism
Brevibacillus brevis (Bacillus brevis)
Status
Functioni
In the first step of peptide synthesis this enzyme activates phenylalanine and racemizes it to the D-isomer.
By similarityMiscellaneous
The racemization reaction takes place in the thioester-bound stage of phenylalanine that is formed via the thiol group of the serine-bound phosphopantetheine.By similarity
Catalytic activityi
- EC:5.1.1.11
Cofactori
pantetheine 4'-phosphateBy similarityNote: Binds 1 phosphopantetheine covalently.By similarity
: gramicidin S biosynthesis Pathwayi
This protein is involved in the pathway gramicidin S biosynthesis, which is part of Antibiotic biosynthesis.View all proteins of this organism that are known to be involved in the pathway gramicidin S biosynthesis and in Antibiotic biosynthesis.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ligase activity Source: UniProtKB-KW
- phenylalanine racemase (ATP-hydrolyzing) activity Source: UniProtKB-EC
GO - Biological processi
- antibiotic biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase, Ligase, Multifunctional enzyme |
Biological process | Antibiotic biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 5.1.1.11, 638 |
SABIO-RKi | P0C062 |
UniPathwayi | UPA00102 |
Names & Taxonomyi
Protein namesi | Recommended name: Gramicidin S synthase 1Alternative name(s): Gramicidin S synthase I Including the following 2 domains: ATP-dependent D-phenylalanine adenylase Short name: D-PheA Alternative name(s): D-phenylalanine activase Phenylalanine racemase [ATP-hydrolyzing] (EC:5.1.1.11) |
Gene namesi | Name:grsA Synonyms:grs1 |
Organismi | Brevibacillus brevis (Bacillus brevis) |
Taxonomic identifieri | 1393 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Brevibacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193086 | 1 – 1098 | Gramicidin S synthase 1Add BLAST | 1098 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 573 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Phosphopantetheine, PhosphoproteinInteractioni
Subunit structurei
Large multienzyme complex of GrsA and GrsB.
By similarityStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0C062 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 538 – 612 | CarrierPROSITE-ProRule annotationAdd BLAST | 75 |
Domaini
One-module-bearing peptide synthase with a C-terminal epimerization domain. Each module incorporates one amino acid into the peptide product and can be further subdivided into domains responsible for substrate adenylation, thiolation, condensation (not for the initiation module), and epimerization (optional), and N methylation (optional) (By similarity).By similarity
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.30.300.30, 1 hit 3.30.559.10, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR036736, ACP-like_sf IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR010060, NRPS_synth IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit PF00668, Condensation, 1 hit PF00550, PP-binding, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit TIGR01720, NRPS-para261, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
i Sequence
Sequence statusi: Complete.
P0C062-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNSSKSILI HAQNKNGTHE EEQYLFAVNN TKAEYPRDKT IHQLFEEQVS
60 70 80 90 100
KRPNNVAIVC ENEQLTYHEL NVKANQLARI FIEKGIGKDT LVGIMMEKSI
110 120 130 140 150
DLFIGILAVL KAGGAYVPID IEYPKERIQY ILDDSQARML LTQKHLVHLI
160 170 180 190 200
HNIQFNGQVE IFEEDTIKIR EGTNLHVPSK STDLAYVIYT SGTTGNPKGT
210 220 230 240 250
MLEHKGISNL KVFFENSLNV TEKDRIGQFA SISFDASVWE MFMALLTGAS
260 270 280 290 300
LYIILKDTIN DFVKFEQYIN QKEITVITLP PTYVVHLDPE RILSIQTLIT
310 320 330 340 350
AGSATSPSLV NKWKEKVTYI NAYGPTETTI CATTWVATKE TTGHSVPIGA
360 370 380 390 400
PIQNTQIYIV DENLQLKSVG EAGELCIGGE GLARGYWKRP ELTSQKFVDN
410 420 430 440 450
PFVPGEKLYK TGDQARWLPD GNIEYLGRID NQVKIRGHRV ELEEVESILL
460 470 480 490 500
KHMYISETAV SVHKDHQEQP YLCAIFVSEK HIPLEQLRQF SSEELPTYMI
510 520 530 540 550
PSYFIQLDKM PLTSNGKIDR KQLPEPDLTF GMRVDYEAPR NEIEETLVTI
560 570 580 590 600
WQDVLGIEKI GIKDNFYALG GDSIKAIQVA ARLHSYQLKL ETKDLLKYPT
610 620 630 640 650
IDQLVHYIKD SKRRSEQGIV EGEIGLTPIQ HWFFEQQFTN MHHWNQSYML
660 670 680 690 700
YRPNGFDKEI LLRVFNKIVE HHDALRMIYK HHNGKIVQIN RGLEGTLFDF
710 720 730 740 750
YTFDLTANDN EQQVICEESA RLQNSINLEV GPLVKIALFH TQNGDHLFMA
760 770 780 790 800
IHHLVVDGIS WRILFEDLAT AYEQAMHQQT IALPEKTDSF KDWSIELEKY
810 820 830 840 850
ANSELFLEEA EYWHHLNYYT DNVQIKKDYV TMNNKQKNIR YVGMELTIEE
860 870 880 890 900
TEKLLKNVNK AYRTEINDIL LTALGFALKE WADIDKIVIN LEGHGREEIL
910 920 930 940 950
EQMNIARTVG WFTSQYPVVL DMQKSDDLSY QIKLMKENLR RIPNKGIGYE
960 970 980 990 1000
IFKYLTTEYL RPVLPFTLKP EINFNYLGQF DTDVKTELFT RSPYSMGNSL
1010 1020 1030 1040 1050
GPDGKNNLSP EGESYFVLNI NGFIEEGKLH ITFSYNEQQY KEDTIQQLSR
1060 1070 1080 1090
SYKQHLLAII EHCVQKEDTE LTPSDFSFKE LELEEMDDIF DLLADSLT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00519 Genomic DNA Translation: BAA00406.1 D00938 Genomic DNA Translation: BAA00777.1 |
PIRi | JU0122, YGBSG1 |
Genome annotation databases
KEGGi | ag:BAA00406 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00519 Genomic DNA Translation: BAA00406.1 D00938 Genomic DNA Translation: BAA00777.1 |
PIRi | JU0122, YGBSG1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ISW | X-ray | 1.75 | A | 538-1098 | [»] | |
5ISX | X-ray | 2.33 | A/B | 538-1098 | [»] | |
SMRi | P0C062 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
KEGGi | ag:BAA00406 |
Enzyme and pathway databases
UniPathwayi | UPA00102 |
BRENDAi | 5.1.1.11, 638 |
SABIO-RKi | P0C062 |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.30.300.30, 1 hit 3.30.559.10, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR036736, ACP-like_sf IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR010060, NRPS_synth IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit PF00668, Condensation, 1 hit PF00550, PP-binding, 1 hit |
SUPFAMi | SSF47336, SSF47336, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit TIGR01720, NRPS-para261, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit PS50075, CARRIER, 1 hit PS00012, PHOSPHOPANTETHEINE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GRSA_BREBE | |
Accessioni | P0C062Primary (citable) accession number: P0C062 Secondary accession number(s): P14687 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | July 5, 2005 | |
Last modified: | February 23, 2022 | |
This is version 74 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families