UniProtKB - P0C058 (IBPB_ECOLI)
Protein
Small heat shock protein IbpB
Gene
ibpB
Organism
Escherichia coli (strain K12)
Status
Functioni
Associates with aggregated proteins, together with IbpA, to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress. Aggregated proteins bound to the IbpAB complex are more efficiently refolded and reactivated by the ATP-dependent chaperone systems ClpB and DnaK/DnaJ/GrpE. Its activity is ATP-independent.3 Publications
Temperature dependencei
Thermostable up to 50 degrees Celsius.
GO - Molecular functioni
- identical protein binding Source: EcoCyc
GO - Biological processi
- protein stabilization Source: UniProtKB-UniRule
- response to heat Source: EcoCyc
- stress response to copper ion Source: EcoCyc
Keywordsi
Molecular function | Chaperone |
Biological process | Stress response |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11535-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Small heat shock protein IbpBAlternative name(s): 16 kDa heat shock protein B |
Gene namesi | Name:ibpB Synonyms:hslS, htpE Ordered Locus Names:b3686, JW3663 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000126028 | 1 – 142 | Small heat shock protein IbpBAdd BLAST | 142 |
Proteomic databases
PaxDbi | P0C058 |
PRIDEi | P0C058 |
Expressioni
Inductioni
By heat shock.1 Publication
Interactioni
Subunit structurei
Homodimer. Forms homomultimers of about 100-150 subunits at optimal growth temperatures. Conformation changes to oligomers at high temperatures or high ionic concentrations. The decrease in size of the multimers is accompanied by an increase in chaperone activity.
3 PublicationsBinary interactionsi
Hide detailsP0C058
With | #Exp. | IntAct |
---|---|---|
ibpA [P0C054] | 5 | EBI-552784,EBI-550729 |
itself | 3 | EBI-552784,EBI-552784 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4260809, 224 interactors 852497, 1 interactor |
DIPi | DIP-48244N |
IntActi | P0C058, 11 interactors |
MINTi | P0C058 |
STRINGi | 511145.b3686 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 137 | sHSPPROSITE-ProRule annotationAdd BLAST | 112 |
Domaini
The N- and C-terminal flexible termini are involved in oligomerization and in the binding of non-native substrate proteins, and are essential for chaperone activity.1 Publication
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0071, Bacteria |
HOGENOMi | CLU_046737_4_2_6 |
InParanoidi | P0C058 |
PhylomeDBi | P0C058 |
Family and domain databases
CDDi | cd06470, ACD_IbpA-B_like, 1 hit |
Gene3Di | 2.60.40.790, 1 hit |
HAMAPi | MF_02001, HSP20_IbpB, 1 hit |
InterProi | View protein in InterPro IPR002068, A-crystallin/Hsp20_dom IPR037913, ACD_IbpA/B IPR008978, HSP20-like_chaperone IPR022848, HSP20_IbpB |
Pfami | View protein in Pfam PF00011, HSP20, 1 hit |
SUPFAMi | SSF49764, SSF49764, 1 hit |
PROSITEi | View protein in PROSITE PS01031, SHSP, 1 hit |
i Sequence
Sequence statusi: Complete.
P0C058-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRNFDLSPLM RQWIGFDKLA NALQNAGESQ SFPPYNIEKS DDNHYRITLA
60 70 80 90 100
LAGFRQEDLE IQLEGTRLSV KGTPEQPKEE KKWLHQGLMN QPFSLSFTLA
110 120 130 140
ENMEVSGATF VNGLLHIDLI RNEPEPIAAQ RIAISERPAL NS
Sequence cautioni
The sequence AAA62038 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94104 Genomic DNA Translation: AAA24425.1 L10328 Genomic DNA Translation: AAA62038.1 Different initiation. U00096 Genomic DNA Translation: AAC76709.2 AP009048 Genomic DNA Translation: BAE77608.1 |
RefSeqi | NP_418141.2, NC_000913.3 WP_001243431.1, NZ_STEB01000015.1 |
Genome annotation databases
EnsemblBacteriai | AAC76709; AAC76709; b3686 BAE77608; BAE77608; BAE77608 |
GeneIDi | 58460850 948192 |
KEGGi | ecj:JW3663 eco:b3686 |
PATRICi | fig|511145.12.peg.3808 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94104 Genomic DNA Translation: AAA24425.1 L10328 Genomic DNA Translation: AAA62038.1 Different initiation. U00096 Genomic DNA Translation: AAC76709.2 AP009048 Genomic DNA Translation: BAE77608.1 |
RefSeqi | NP_418141.2, NC_000913.3 WP_001243431.1, NZ_STEB01000015.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260809, 224 interactors 852497, 1 interactor |
DIPi | DIP-48244N |
IntActi | P0C058, 11 interactors |
MINTi | P0C058 |
STRINGi | 511145.b3686 |
Proteomic databases
PaxDbi | P0C058 |
PRIDEi | P0C058 |
Genome annotation databases
EnsemblBacteriai | AAC76709; AAC76709; b3686 BAE77608; BAE77608; BAE77608 |
GeneIDi | 58460850 948192 |
KEGGi | ecj:JW3663 eco:b3686 |
PATRICi | fig|511145.12.peg.3808 |
Organism-specific databases
EchoBASEi | EB1497 |
Phylogenomic databases
eggNOGi | COG0071, Bacteria |
HOGENOMi | CLU_046737_4_2_6 |
InParanoidi | P0C058 |
PhylomeDBi | P0C058 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11535-MONOMER |
Miscellaneous databases
PROi | PR:P0C058 |
Family and domain databases
CDDi | cd06470, ACD_IbpA-B_like, 1 hit |
Gene3Di | 2.60.40.790, 1 hit |
HAMAPi | MF_02001, HSP20_IbpB, 1 hit |
InterProi | View protein in InterPro IPR002068, A-crystallin/Hsp20_dom IPR037913, ACD_IbpA/B IPR008978, HSP20-like_chaperone IPR022848, HSP20_IbpB |
Pfami | View protein in Pfam PF00011, HSP20, 1 hit |
SUPFAMi | SSF49764, SSF49764, 1 hit |
PROSITEi | View protein in PROSITE PS01031, SHSP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IBPB_ECOLI | |
Accessioni | P0C058Primary (citable) accession number: P0C058 Secondary accession number(s): P29210, P76733, Q2M7Z8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | July 5, 2005 | |
Last modified: | February 10, 2021 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families