UniProtKB - P0C037 (PPNP_ECOLI)
Protein
Pyrimidine/purine nucleoside phosphorylase
Gene
ppnP
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases. Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates. Also catalyzes the reverse reactions. Is not able to produce D-ribose 1-phosphate from D-ribose and phosphate.1 Publication
Catalytic activityi
- EC:2.4.2.11 Publication
- EC:2.4.2.11 Publication
- EC:2.4.2.11 Publication EC:2.4.2.151 Publication
- EC:2.4.2.11 Publication
- EC:2.4.2.21 Publication EC:2.4.2.41 Publication
- EC:2.4.2.21 Publication EC:2.4.2.31 Publication
- EC:2.4.2.11 Publication
GO - Molecular functioni
- guanosine phosphorylase activity Source: UniProtKB-EC
- purine-nucleoside phosphorylase activity Source: EcoCyc
- pyrimidine-nucleoside phosphorylase activity Source: EcoCyc
- thymidine phosphorylase activity Source: UniProtKB-EC
- uridine phosphorylase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12159-MONOMER MetaCyc:EG12159-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Pyrimidine/purine nucleoside phosphorylase1 PublicationUniRule annotation (EC:2.4.2.1UniRule annotation1 Publication, EC:2.4.2.2UniRule annotation1 Publication)Alternative name(s): Adenosine phosphorylase1 Publication Cytidine phosphorylase1 Publication Guanosine phosphorylase1 Publication (EC:2.4.2.151 Publication) Inosine phosphorylase1 Publication Thymidine phosphorylase1 Publication (EC:2.4.2.41 Publication) Uridine phosphorylase1 Publication (EC:2.4.2.31 Publication) Xanthosine phosphorylase1 Publication |
Gene namesi | Name:ppnP1 Publication Synonyms:yaiEImported Ordered Locus Names:b0391, JW0382 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene show a consistent change in xanthine level.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211763 | 1 – 94 | Pyrimidine/purine nucleoside phosphorylaseAdd BLAST | 94 |
Proteomic databases
jPOSTi | P0C037 |
PaxDbi | P0C037 |
PRIDEi | P0C037 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259817, 22 interactors 849437, 8 interactors |
IntActi | P0C037, 9 interactors |
STRINGi | 511145.b0391 |
Family & Domainsi
Sequence similaritiesi
Belongs to the nucleoside phosphorylase PpnP family.UniRule annotation
Phylogenomic databases
eggNOGi | COG3123, Bacteria |
HOGENOMi | CLU_157874_0_0_6 |
InParanoidi | P0C037 |
PhylomeDBi | P0C037 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
HAMAPi | MF_01537, Nucleos_phosphorylase_PpnP, 1 hit |
InterProi | View protein in InterPro IPR009664, Ppnp IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
PANTHERi | PTHR36540, PTHR36540, 1 hit |
Pfami | View protein in Pfam PF06865, Ppnp, 1 hit |
SUPFAMi | SSF51182, SSF51182, 1 hit |
i Sequence
Sequence statusi: Complete.
P0C037-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLQSNEYFSG KVKSIGFSSS STGRASVGVM VEGEYTFSTA EPEEMTVISG
60 70 80 90
ALNVLLPDAT DWQVYEAGSV FNVPGHSEFH LQVAEPTSYL CRYL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 77 – 94 | SEFHL…LCRYL → TVSFICKLPNPPLICAAICN SSPSP in CAA54286 (PubMed:8807285).CuratedAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76979 Genomic DNA Translation: CAA54286.1 U73857 Genomic DNA Translation: AAB18115.1 U00096 Genomic DNA Translation: AAC73494.1 AP009048 Genomic DNA Translation: BAE76172.1 |
PIRi | G64767 |
RefSeqi | NP_414925.1, NC_000913.3 WP_000941942.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73494; AAC73494; b0391 BAE76172; BAE76172; BAE76172 |
GeneIDi | 58460366 945048 |
KEGGi | ecj:JW0382 eco:b0391 |
PATRICi | fig|511145.12.peg.403 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76979 Genomic DNA Translation: CAA54286.1 U73857 Genomic DNA Translation: AAB18115.1 U00096 Genomic DNA Translation: AAC73494.1 AP009048 Genomic DNA Translation: BAE76172.1 |
PIRi | G64767 |
RefSeqi | NP_414925.1, NC_000913.3 WP_000941942.1, NZ_STEB01000007.1 |
3D structure databases
SMRi | P0C037 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259817, 22 interactors 849437, 8 interactors |
IntActi | P0C037, 9 interactors |
STRINGi | 511145.b0391 |
Proteomic databases
jPOSTi | P0C037 |
PaxDbi | P0C037 |
PRIDEi | P0C037 |
Genome annotation databases
EnsemblBacteriai | AAC73494; AAC73494; b0391 BAE76172; BAE76172; BAE76172 |
GeneIDi | 58460366 945048 |
KEGGi | ecj:JW0382 eco:b0391 |
PATRICi | fig|511145.12.peg.403 |
Organism-specific databases
EchoBASEi | EB2079 |
Phylogenomic databases
eggNOGi | COG3123, Bacteria |
HOGENOMi | CLU_157874_0_0_6 |
InParanoidi | P0C037 |
PhylomeDBi | P0C037 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12159-MONOMER MetaCyc:EG12159-MONOMER |
Miscellaneous databases
PROi | PR:P0C037 |
Family and domain databases
Gene3Di | 2.60.120.10, 1 hit |
HAMAPi | MF_01537, Nucleos_phosphorylase_PpnP, 1 hit |
InterProi | View protein in InterPro IPR009664, Ppnp IPR014710, RmlC-like_jellyroll IPR011051, RmlC_Cupin_sf |
PANTHERi | PTHR36540, PTHR36540, 1 hit |
Pfami | View protein in Pfam PF06865, Ppnp, 1 hit |
SUPFAMi | SSF51182, SSF51182, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPNP_ECOLI | |
Accessioni | P0C037Primary (citable) accession number: P0C037 Secondary accession number(s): P36768, P77343, Q2MC34 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | July 5, 2005 | |
Last modified: | February 10, 2021 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - SIMILARITY comments
Index of protein domains and families