rsmE

Functionⁱ

Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.2 Publications

Catalytic activityⁱ

S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N₃-methyluracil(1498) in 16S rRNA.1 Publication

Kineticsⁱ

K_M=
2.0 µM for 16S rRNA within 30S ribosomal subunits
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE."
  Basturea G.N., Deutscher M.P.
  RNA 13:1969-1976(2007) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
K_M=
26.7 µM for S-adenosyl-L-methionine
1 Publication
Manual assertion based on experiment inⁱ
- Ref.7
  "Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE."
  Basturea G.N., Deutscher M.P.
  RNA 13:1969-1976(2007) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

pH dependenceⁱ

Optimum pH is 7-9.1 Publication

GO - Molecular functionⁱ

rRNA (uridine-N3-)-methyltransferase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 16431987

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

rRNA base methylation
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 16431987

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Biological process	rRNA processing
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG12366-MONOMER MetaCyc:EG12366-MONOMER
BRENDAⁱ	2.1.1.193 2026

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ribosomal RNA small subunit methyltransferase E (EC:2.1.1.193) Alternative name(s): 16S rRNA m3U1498 methyltransferase
Gene namesⁱ	Name:rsmE Synonyms:yggJ Ordered Locus Names:b2946, JW2913
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG12366 rsmE

EcoGeneⁱ

EG12366 rsmE

Subcellular locationⁱ

Cytoplasm Curated

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000176199	1 – 243	Ribosomal RNA small subunit methyltransferase EAdd BLAST		243

Proteomic databases

PaxDbⁱ	P0AGL7
PRIDEⁱ	P0AGL7

Interactionⁱ

Subunit structureⁱ

Homodimer.1 Publication

Protein-protein interaction databases

BioGridⁱ	4259407, 22 interactors
IntActⁱ	P0AGL7, 5 interactors
STRINGⁱ	316385.ECDH10B_3121

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	5 – 7	Combined sources	3
Beta strandⁱ	17 – 19	Combined sources	3
Helixⁱ	22 – 29	Combined sources	8
Beta strandⁱ	39 – 43	Combined sources	5
Beta strandⁱ	45 – 57	Combined sources	13
Beta strandⁱ	62 – 71	Combined sources	10
Beta strandⁱ	77 – 85	Combined sources	9
Beta strandⁱ	88 – 90	Combined sources	3
Helixⁱ	91 – 101	Combined sources	11
Beta strandⁱ	106 – 111	Combined sources	6
Helixⁱ	121 – 142	Combined sources	22
Helixⁱ	156 – 161	Combined sources	6
Beta strandⁱ	165 – 171	Combined sources	7
Beta strandⁱ	176 – 178	Combined sources	3
Helixⁱ	179 – 181	Combined sources	3
Beta strandⁱ	188 – 193	Combined sources	6
Helixⁱ	201 – 209	Combined sources	9
Beta strandⁱ	213 – 216	Combined sources	4
Helixⁱ	224 – 238	Combined sources	15

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4E8B	X-ray	2.25	A	1-243	[»]
ProteinModelPortalⁱ	P0AGL7
SMRⁱ	P0AGL7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the RNA methyltransferase RsmE family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105QF0 Bacteria COG1385 LUCA
HOGENOMⁱ	HOG000015265
InParanoidⁱ	P0AGL7
KEGG Orthology (KO) More...KOⁱ	K09761
OMAⁱ	ERMEFTI
PhylomeDBⁱ	P0AGL7

Family and domain databases

Gene3Dⁱ	3.40.1280.10, 1 hit
InterProⁱ	View protein in InterPro IPR029028 Alpha/beta_knot_MTases IPR015947 PUA-like_sf IPR006700 rRNA_ssu_MeTrfase-E IPR029026 tRNA_m1G_MTases_N
PANTHERⁱ	PTHR30027 PTHR30027, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04452 Methyltrans_RNA, 1 hit
PIRSFⁱ	PIRSF015601 MTase_slr0722, 1 hit
SUPFAMⁱ	SSF75217 SSF75217, 1 hit SSF88697 SSF88697, 1 hit
TIGRFAMsⁱ	TIGR00046 TIGR00046, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AGL7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRIPRIYHPE PLTSHSHIAL CEDAANHIGR VLRMGPGQAL QLFDGSNQVF 
        60         70         80         90        100
DAEITSASKK SVEVKVLEGQ IDDRESPLHI HLGQVMSRGE KMEFTIQKSI 
       110        120        130        140        150
ELGVSLITPL FSERCGVKLD SERLNKKLQQ WQKIAIAACE QCGRNRVPEI 
       160        170        180        190        200
RPAMDLEAWC AEQDEGLKLN LHPRASNSIN TLPLPVERVR LLIGPEGGLS 
       210        220        230        240 
ADEIAMTARY QFTDILLGPR VLRTETTALT AITALQVRFG DLG

Length:243

Mass (Da):26,978

Last modified:December 20, 2005 - v1

Checksum:ⁱ5C4659F5B295E033

GO

Sequence cautionⁱ

The sequence AAA69113 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U28377 Genomic DNA Translation: AAA69113.1 Different initiation. U00096 Genomic DNA Translation: AAC75983.2 AP009048 Genomic DNA Translation: BAE77009.1 X65169 Genomic DNA No translation available. X01666 Genomic DNA No translation available.
NCBI Reference Sequences More...RefSeqⁱ	NP_417421.4, NC_000913.3 WP_001222509.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC75983; AAC75983; b2946 BAE77009; BAE77009; BAE77009
GeneIDⁱ	945816
KEGGⁱ	ecj:JW2913 eco:b2946
PATRICⁱ	fig\|1411691.4.peg.3787

Protein	Similar proteins		Species	Score	Length
P0AGL7	Ribosomal RNA small subunit methyltransferase E		ECOL6		243
Ribosomal RNA small subunit methyltransferase E		SHIFL		243
Ribosomal RNA small subunit methyltransferase E		Klebsiella pneumoniae IS22		243
Ribosomal RNA small subunit methyltransferase E		Escherichia coli D6-113.11		243
Ribosomal RNA small subunit methyltransferase E		Shigella flexneri 1235-66		243
+578

Protein	Similar proteins		Species	Score	Length
P0AGL7	Ribosomal RNA small subunit methyltransferase E		ECOL6		243
Ribosomal RNA small subunit methyltransferase E		SHIFL		243
Ribosomal RNA small subunit methyltransferase E		Klebsiella pneumoniae IS22		243
Ribosomal RNA small subunit methyltransferase E		Escherichia coli D6-113.11		243
Ribosomal RNA small subunit methyltransferase E		Shigella flexneri 1235-66		243
+746

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U28377 Genomic DNA Translation: AAA69113.1 Different initiation. U00096 Genomic DNA Translation: AAC75983.2 AP009048 Genomic DNA Translation: BAE77009.1 X65169 Genomic DNA No translation available. X01666 Genomic DNA No translation available.
NCBI Reference Sequences More...RefSeqⁱ	NP_417421.4, NC_000913.3 WP_001222509.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4E8B	X-ray	2.25	A	1-243	[»]
ProteinModelPortalⁱ	P0AGL7
SMRⁱ	P0AGL7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259407, 22 interactors
IntActⁱ	P0AGL7, 5 interactors
STRINGⁱ	316385.ECDH10B_3121

Proteomic databases

PaxDbⁱ	P0AGL7
PRIDEⁱ	P0AGL7

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC75983; AAC75983; b2946 BAE77009; BAE77009; BAE77009
GeneIDⁱ	945816
KEGGⁱ	ecj:JW2913 eco:b2946
PATRICⁱ	fig\|1411691.4.peg.3787

Organism-specific databases

EchoBASEⁱ	EB2269
EcoGeneⁱ	EG12366 rsmE

Phylogenomic databases

eggNOGⁱ	ENOG4105QF0 Bacteria COG1385 LUCA
HOGENOMⁱ	HOG000015265
InParanoidⁱ	P0AGL7
KEGG Orthology (KO) More...KOⁱ	K09761
OMAⁱ	ERMEFTI
PhylomeDBⁱ	P0AGL7

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG12366-MONOMER MetaCyc:EG12366-MONOMER
BRENDAⁱ	2.1.1.193 2026

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0AGL7

PROⁱ

PR:P0AGL7

Family and domain databases

Gene3Dⁱ	3.40.1280.10, 1 hit
InterProⁱ	View protein in InterPro IPR029028 Alpha/beta_knot_MTases IPR015947 PUA-like_sf IPR006700 rRNA_ssu_MeTrfase-E IPR029026 tRNA_m1G_MTases_N
PANTHERⁱ	PTHR30027 PTHR30027, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04452 Methyltrans_RNA, 1 hit
PIRSFⁱ	PIRSF015601 MTase_slr0722, 1 hit
SUPFAMⁱ	SSF75217 SSF75217, 1 hit SSF88697 SSF88697, 1 hit
TIGRFAMsⁱ	TIGR00046 TIGR00046, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RSME_ECOLI
Accessionⁱ	P0AGL7Primary (citable) accession number: P0AGL7 Secondary accession number(s): P37912, P76647, Q2M9P7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
	Last sequence update:	December 20, 2005
	Last modified:	March 28, 2018
	This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries

UniProtKB

UniParc

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UniRef

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Supporting data

UniProtKB - P0AGL7 (RSME_ECOLI)

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Ribosomal RNA small subunit methyltransferase E

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Kineticsⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ