UniProtKB - P0AGL7 (RSME_ECOLI)
Protein
Ribosomal RNA small subunit methyltransferase E
Gene
rsmE
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.2 Publications
Catalytic activityi
- S-adenosyl-L-methionine + uridine1498 in 16S rRNA = H+ + N3-methyluridine1498 in 16S rRNA + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1931 Publication
Kineticsi
- KM=2.0 µM for 16S rRNA within 30S ribosomal subunits1 Publication
- KM=26.7 µM for S-adenosyl-L-methionine1 Publication
pH dependencei
Optimum pH is 7-9.1 Publication
GO - Molecular functioni
- rRNA (uridine-N3-)-methyltransferase activity Source: EcoCyc
GO - Biological processi
- rRNA base methylation Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12366-MONOMER MetaCyc:EG12366-MONOMER |
BRENDAi | 2.1.1.193, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA small subunit methyltransferase E (EC:2.1.1.193)Alternative name(s): 16S rRNA m3U1498 methyltransferase |
Gene namesi | Name:rsmE Synonyms:yggJ Ordered Locus Names:b2946, JW2913 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000176199 | 1 – 243 | Ribosomal RNA small subunit methyltransferase EAdd BLAST | 243 |
Proteomic databases
jPOSTi | P0AGL7 |
PaxDbi | P0AGL7 |
PRIDEi | P0AGL7 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259407, 22 interactors |
IntActi | P0AGL7, 5 interactors |
STRINGi | 511145.b2946 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AGL7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the RNA methyltransferase RsmE family.Curated
Phylogenomic databases
eggNOGi | COG1385, Bacteria |
HOGENOMi | CLU_067442_5_1_6 |
InParanoidi | P0AGL7 |
PhylomeDBi | P0AGL7 |
Family and domain databases
Gene3Di | 3.40.1280.10, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR015947, PUA-like_sf IPR006700, rRNA_ssu_MeTrfase-E IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR30027, PTHR30027, 1 hit |
Pfami | View protein in Pfam PF04452, Methyltrans_RNA, 1 hit |
PIRSFi | PIRSF015601, MTase_slr0722, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00046, TIGR00046, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AGL7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRIPRIYHPE PLTSHSHIAL CEDAANHIGR VLRMGPGQAL QLFDGSNQVF
60 70 80 90 100
DAEITSASKK SVEVKVLEGQ IDDRESPLHI HLGQVMSRGE KMEFTIQKSI
110 120 130 140 150
ELGVSLITPL FSERCGVKLD SERLNKKLQQ WQKIAIAACE QCGRNRVPEI
160 170 180 190 200
RPAMDLEAWC AEQDEGLKLN LHPRASNSIN TLPLPVERVR LLIGPEGGLS
210 220 230 240
ADEIAMTARY QFTDILLGPR VLRTETTALT AITALQVRFG DLG
Sequence cautioni
The sequence AAA69113 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69113.1 Different initiation. U00096 Genomic DNA Translation: AAC75983.2 AP009048 Genomic DNA Translation: BAE77009.1 X65169 Genomic DNA No translation available. X01666 Genomic DNA No translation available. |
RefSeqi | NP_417421.4, NC_000913.3 WP_001222509.1, NZ_SSUR01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC75983; AAC75983; b2946 BAE77009; BAE77009; BAE77009 |
GeneIDi | 58389770 945816 |
KEGGi | ecj:JW2913 eco:b2946 |
PATRICi | fig|1411691.4.peg.3787 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28377 Genomic DNA Translation: AAA69113.1 Different initiation. U00096 Genomic DNA Translation: AAC75983.2 AP009048 Genomic DNA Translation: BAE77009.1 X65169 Genomic DNA No translation available. X01666 Genomic DNA No translation available. |
RefSeqi | NP_417421.4, NC_000913.3 WP_001222509.1, NZ_SSUR01000013.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4E8B | X-ray | 2.25 | A | 1-243 | [»] | |
SMRi | P0AGL7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259407, 22 interactors |
IntActi | P0AGL7, 5 interactors |
STRINGi | 511145.b2946 |
Proteomic databases
jPOSTi | P0AGL7 |
PaxDbi | P0AGL7 |
PRIDEi | P0AGL7 |
Genome annotation databases
EnsemblBacteriai | AAC75983; AAC75983; b2946 BAE77009; BAE77009; BAE77009 |
GeneIDi | 58389770 945816 |
KEGGi | ecj:JW2913 eco:b2946 |
PATRICi | fig|1411691.4.peg.3787 |
Organism-specific databases
EchoBASEi | EB2269 |
Phylogenomic databases
eggNOGi | COG1385, Bacteria |
HOGENOMi | CLU_067442_5_1_6 |
InParanoidi | P0AGL7 |
PhylomeDBi | P0AGL7 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12366-MONOMER MetaCyc:EG12366-MONOMER |
BRENDAi | 2.1.1.193, 2026 |
Miscellaneous databases
PROi | PR:P0AGL7 |
Family and domain databases
Gene3Di | 3.40.1280.10, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR015947, PUA-like_sf IPR006700, rRNA_ssu_MeTrfase-E IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR30027, PTHR30027, 1 hit |
Pfami | View protein in Pfam PF04452, Methyltrans_RNA, 1 hit |
PIRSFi | PIRSF015601, MTase_slr0722, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00046, TIGR00046, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RSME_ECOLI | |
Accessioni | P0AGL7Primary (citable) accession number: P0AGL7 Secondary accession number(s): P37912, P76647, Q2M9P7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families