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Entry version 107 (12 Aug 2020)
Sequence version 1 (20 Dec 2005)
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Protein

tRNA (guanosine(18)-2'-O)-methyltransferase

Gene

trmH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the 2'-O methylation of guanosine at position 18 in tRNA. Type II methylase, which methylates only a subset of tRNA species.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei96S-adenosyl-L-methionineUniRule annotation1
Binding sitei139S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei148S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • tRNA guanine ribose methylation Source: EcoCyc
  • tRNA methylation Source: EcoliWiki

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG10967-MONOMER
ECOL316407:JW3626-MONOMER
MetaCyc:EG10967-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
tRNA (guanosine(18)-2'-O)-methyltransferaseUniRule annotationCurated (EC:2.1.1.34UniRule annotation1 Publication)
Alternative name(s):
tRNA [Gm18] methyltransferaseUniRule annotationCurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:trmH1 PublicationUniRule annotation
Synonyms:spoU1 Publication
Ordered Locus Names:b3651, JW3626
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001597721 – 229tRNA (guanosine(18)-2'-O)-methyltransferaseAdd BLAST229

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P0AGJ2

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P0AGJ2

PRoteomics IDEntifications database

More...
PRIDEi
P0AGJ2

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4262569, 162 interactors
852468, 7 interactors

Database of interacting proteins

More...
DIPi
DIP-35977N

Protein interaction database and analysis system

More...
IntActi
P0AGJ2, 11 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b3651

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P0AGJ2

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0566, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_021322_4_2_6

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
P0AGJ2

KEGG Orthology (KO)

More...
KOi
K00556

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P0AGJ2

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
3.40.1280.10, 1 hit

HAMAP database of protein families

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HAMAPi
MF_02060, tRNA_methyltr_TrmH, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR029028, Alpha/beta_knot_MTases
IPR022724, rRNA_MeTrfase_SpoU_C
IPR001537, SpoU_MeTrfase
IPR033671, TrmH
IPR029026, tRNA_m1G_MTases_N

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF12105, SpoU_methylas_C, 1 hit
PF00588, SpoU_methylase, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF75217, SSF75217, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AGJ2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNPTRYARIC EMLARRQPDL TVCMEQVHKP HNVSAIIRTA DAVGVHEVHA
60 70 80 90 100
VWPGSRMRTM ASAAAGSNSW VQVKTHRTIG DAVAHLKGQG MQILATHLSD
110 120 130 140 150
NAVDFREIDY TRPTCILMGQ EKTGITQEAL ALADQDIIIP MIGMVQSLNV
160 170 180 190 200
SVASALILYE AQRQRQNAGM YLRENSMLPE AEQQRLLFEG GYPVLAKVAK
210 220
RKGLPYPHVN QQGEIEADAD WWATMQAAG
Length:229
Mass (Da):25,343
Last modified:December 20, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBC5AB6B804BDEE2E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M24503 Genomic DNA Translation: AAB00161.1
L10328 Genomic DNA Translation: AAA62004.1
U00096 Genomic DNA Translation: AAC76675.1
AP009048 Genomic DNA Translation: BAE77642.1

Protein sequence database of the Protein Information Resource

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PIRi
JV0043

NCBI Reference Sequences

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RefSeqi
NP_418108.1, NC_000913.3
WP_001070177.1, NZ_STEB01000024.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC76675; AAC76675; b3651
BAE77642; BAE77642; BAE77642

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
48133463
948161

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW3626
eco:b3651

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.3055

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24503 Genomic DNA Translation: AAB00161.1
L10328 Genomic DNA Translation: AAA62004.1
U00096 Genomic DNA Translation: AAC76675.1
AP009048 Genomic DNA Translation: BAE77642.1
PIRiJV0043
RefSeqiNP_418108.1, NC_000913.3
WP_001070177.1, NZ_STEB01000024.1

3D structure databases

SMRiP0AGJ2
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4262569, 162 interactors
852468, 7 interactors
DIPiDIP-35977N
IntActiP0AGJ2, 11 interactors
STRINGi511145.b3651

Proteomic databases

jPOSTiP0AGJ2
PaxDbiP0AGJ2
PRIDEiP0AGJ2

Genome annotation databases

EnsemblBacteriaiAAC76675; AAC76675; b3651
BAE77642; BAE77642; BAE77642
GeneIDi48133463
948161
KEGGiecj:JW3626
eco:b3651
PATRICifig|1411691.4.peg.3055

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0960

Phylogenomic databases

eggNOGiCOG0566, Bacteria
HOGENOMiCLU_021322_4_2_6
InParanoidiP0AGJ2
KOiK00556
PhylomeDBiP0AGJ2

Enzyme and pathway databases

BioCyciEcoCyc:EG10967-MONOMER
ECOL316407:JW3626-MONOMER
MetaCyc:EG10967-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P0AGJ2

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
HAMAPiMF_02060, tRNA_methyltr_TrmH, 1 hit
InterProiView protein in InterPro
IPR029028, Alpha/beta_knot_MTases
IPR022724, rRNA_MeTrfase_SpoU_C
IPR001537, SpoU_MeTrfase
IPR033671, TrmH
IPR029026, tRNA_m1G_MTases_N
PfamiView protein in Pfam
PF12105, SpoU_methylas_C, 1 hit
PF00588, SpoU_methylase, 1 hit
SUPFAMiSSF75217, SSF75217, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTRMH_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AGJ2
Secondary accession number(s): P19396, Q2M7W4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: August 12, 2020
This is version 107 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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