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UniProtKB - P0AGJ2 (TRMH_ECOLI)
Protein
tRNA (guanosine(18)-2'-O)-methyltransferase
Gene
trmH
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the 2'-O methylation of guanosine at position 18 in tRNA. Type II methylase, which methylates only a subset of tRNA species.
1 PublicationCatalytic activityi
- guanosine18 in tRNA + S-adenosyl-L-methionine = 2'-O-methylguanosine18 in tRNA + H+ + S-adenosyl-L-homocysteineUniRule annotation1 PublicationEC:2.1.1.34UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 96 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 139 | S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 148 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotation | 1 |
GO - Molecular functioni
- tRNA (guanosine-2'-O-)-methyltransferase activity Source: EcoCyc
- tRNA (uracil-2'-O-)-methyltransferase activity Source: UniProtKB-EC
- tRNA binding Source: UniProtKB-UniRule
GO - Biological processi
- tRNA guanine ribose methylation Source: EcoCyc
- tRNA methylation Source: EcoliWiki
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase, tRNA-binding |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10967-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanosine(18)-2'-O)-methyltransferaseUniRule annotationCurated (EC:2.1.1.34UniRule annotation1 Publication)Alternative name(s): tRNA [Gm18] methyltransferaseUniRule annotationCurated |
Gene namesi | Name:trmH1 PublicationUniRule annotation Synonyms:spoU1 Publication Ordered Locus Names:b3651, JW3626 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159772 | 1 – 229 | tRNA (guanosine(18)-2'-O)-methyltransferaseAdd BLAST | 229 |
Proteomic databases
jPOSTi | P0AGJ2 |
PaxDbi | P0AGJ2 |
PRIDEi | P0AGJ2 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262569, 162 interactors 852468, 7 interactors |
DIPi | DIP-35977N |
IntActi | P0AGJ2, 11 interactors |
STRINGi | 511145.b3651 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0566, Bacteria |
HOGENOMi | CLU_021322_4_2_6 |
InParanoidi | P0AGJ2 |
OMAi | TDRYQHV |
PhylomeDBi | P0AGJ2 |
Family and domain databases
CDDi | cd18092, SpoU-like_TrmH, 1 hit |
Gene3Di | 3.40.1280.10, 1 hit |
HAMAPi | MF_02060, tRNA_methyltr_TrmH, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR022724, rRNA_MeTrfase_SpoU_C IPR001537, SpoU_MeTrfase IPR033671, TrmH IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR43453, PTHR43453, 1 hit |
Pfami | View protein in Pfam PF12105, SpoU_methylas_C, 1 hit PF00588, SpoU_methylase, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AGJ2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNPTRYARIC EMLARRQPDL TVCMEQVHKP HNVSAIIRTA DAVGVHEVHA
60 70 80 90 100
VWPGSRMRTM ASAAAGSNSW VQVKTHRTIG DAVAHLKGQG MQILATHLSD
110 120 130 140 150
NAVDFREIDY TRPTCILMGQ EKTGITQEAL ALADQDIIIP MIGMVQSLNV
160 170 180 190 200
SVASALILYE AQRQRQNAGM YLRENSMLPE AEQQRLLFEG GYPVLAKVAK
210 220
RKGLPYPHVN QQGEIEADAD WWATMQAAG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24503 Genomic DNA Translation: AAB00161.1 L10328 Genomic DNA Translation: AAA62004.1 U00096 Genomic DNA Translation: AAC76675.1 AP009048 Genomic DNA Translation: BAE77642.1 |
PIRi | JV0043 |
RefSeqi | NP_418108.1, NC_000913.3 WP_001070177.1, NZ_STEB01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC76675; AAC76675; b3651 BAE77642; BAE77642; BAE77642 |
GeneIDi | 66672454 948161 |
KEGGi | ecj:JW3626 eco:b3651 |
PATRICi | fig|1411691.4.peg.3055 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24503 Genomic DNA Translation: AAB00161.1 L10328 Genomic DNA Translation: AAA62004.1 U00096 Genomic DNA Translation: AAC76675.1 AP009048 Genomic DNA Translation: BAE77642.1 |
PIRi | JV0043 |
RefSeqi | NP_418108.1, NC_000913.3 WP_001070177.1, NZ_STEB01000024.1 |
3D structure databases
AlphaFoldDBi | P0AGJ2 |
SMRi | P0AGJ2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262569, 162 interactors 852468, 7 interactors |
DIPi | DIP-35977N |
IntActi | P0AGJ2, 11 interactors |
STRINGi | 511145.b3651 |
Proteomic databases
jPOSTi | P0AGJ2 |
PaxDbi | P0AGJ2 |
PRIDEi | P0AGJ2 |
Genome annotation databases
EnsemblBacteriai | AAC76675; AAC76675; b3651 BAE77642; BAE77642; BAE77642 |
GeneIDi | 66672454 948161 |
KEGGi | ecj:JW3626 eco:b3651 |
PATRICi | fig|1411691.4.peg.3055 |
Organism-specific databases
EchoBASEi | EB0960 |
Phylogenomic databases
eggNOGi | COG0566, Bacteria |
HOGENOMi | CLU_021322_4_2_6 |
InParanoidi | P0AGJ2 |
OMAi | TDRYQHV |
PhylomeDBi | P0AGJ2 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10967-MONOMER |
Miscellaneous databases
PROi | PR:P0AGJ2 |
Family and domain databases
CDDi | cd18092, SpoU-like_TrmH, 1 hit |
Gene3Di | 3.40.1280.10, 1 hit |
HAMAPi | MF_02060, tRNA_methyltr_TrmH, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR022724, rRNA_MeTrfase_SpoU_C IPR001537, SpoU_MeTrfase IPR033671, TrmH IPR029026, tRNA_m1G_MTases_N |
PANTHERi | PTHR43453, PTHR43453, 1 hit |
Pfami | View protein in Pfam PF12105, SpoU_methylas_C, 1 hit PF00588, SpoU_methylase, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRMH_ECOLI | |
Accessioni | P0AGJ2Primary (citable) accession number: P0AGJ2 Secondary accession number(s): P19396, Q2M7W4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | May 25, 2022 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families