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Protein

Putrescine export system permease protein SapB

Gene

sapB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of a putrescine export transport system, does not play a role in resistance to antimicrobial peptides.1 Publication

GO - Molecular functioni

  • dipeptide transmembrane transporter activity Source: GO_Central
  • putrescine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:SAPB-MONOMER
MetaCyc:SAPB-MONOMER

Protein family/group databases

TCDBi3.A.1.5.42 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine export system permease protein SapBCurated
Gene namesi
Name:sapB
Ordered Locus Names:b1293, JW1286
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20255 sapB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini30 – 80PeriplasmicSequence analysisAdd BLAST51
Transmembranei81 – 101HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini102 – 112CytoplasmicSequence analysisAdd BLAST11
Transmembranei113 – 133HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini134 – 174PeriplasmicSequence analysisAdd BLAST41
Transmembranei175 – 195HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini196 – 248CytoplasmicSequence analysisAdd BLAST53
Transmembranei249 – 269HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini270 – 280PeriplasmicSequence analysisAdd BLAST11
Transmembranei281 – 301HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini302 – 321CytoplasmicSequence analysis1 PublicationAdd BLAST20

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Single mutation, decreased extracellular putrescine, in an sapBCDF operon deletion no change in resistance to antimicrobial peptide LL-37. Operon deletion has no effect on growth, but if the putrescine importer PuuP is also deleted then growth is slower.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000601581 – 321Putrescine export system permease protein SapBAdd BLAST321

Proteomic databases

PaxDbiP0AGH3
PRIDEiP0AGH3

Interactioni

Protein-protein interaction databases

BioGridi4260136, 262 interactors
STRINGi316385.ECDH10B_1410

Structurei

3D structure databases

ProteinModelPortaliP0AGH3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 302ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST229

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108I5W Bacteria
COG4168 LUCA
HOGENOMiHOG000170242
InParanoidiP0AGH3
KOiK19227
PhylomeDBiP0AGH3

Family and domain databases

CDDicd06261 TM_PBP2, 1 hit
InterProiView protein in InterPro
IPR000515 MetI-like
IPR035906 MetI-like_sf
PfamiView protein in Pfam
PF00528 BPD_transp_1, 1 hit
SUPFAMiSSF161098 SSF161098, 1 hit
PROSITEiView protein in PROSITE
PS50928 ABC_TM1, 1 hit

Sequencei

Sequence statusi: Complete.

P0AGH3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIIFTLRRIL LLIVTLFLLT FVGFSLSYFT PHAPLQGASL WNAWVFWFNG
60 70 80 90 100
LIHWDFGVSS INGQPIAEQL KEVFPATMEL CILAFGFALI VGIPVGMIAG
110 120 130 140 150
ITRHKWQDNL INAIALLGFS IPVFWLALLL TLFCSLTLGW LPVSGRFDLL
160 170 180 190 200
YEVKPITGFA LIDAWLSDSP WRDEMIMSAI RHMILPVITL SVAPTTEVIR
210 220 230 240 250
LMRISTIEVY DQNYVKAAAT RGLSRFTILR RHVLHNALPP VIPRLGLQFS
260 270 280 290 300
TMLTLAMITE MVFSWPGLGR WLINAIRQQD YAAISAGVMV CGSLVIIVNV
310 320
ISDILGAMAN PLKHKEWYAL R
Length:321
Mass (Da):36,038
Last modified:December 20, 2005 - v1
Checksum:iE50607A4A2C551F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97282 Genomic DNA Translation: CAA65938.1
U00096 Genomic DNA Translation: AAC74375.1
AP009048 Genomic DNA Translation: BAA14854.1
PIRiH64877
RefSeqiNP_415809.1, NC_000913.3
WP_000583277.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74375; AAC74375; b1293
BAA14854; BAA14854; BAA14854
GeneIDi946191
KEGGiecj:JW1286
eco:b1293
PATRICifig|1411691.4.peg.986

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97282 Genomic DNA Translation: CAA65938.1
U00096 Genomic DNA Translation: AAC74375.1
AP009048 Genomic DNA Translation: BAA14854.1
PIRiH64877
RefSeqiNP_415809.1, NC_000913.3
WP_000583277.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP0AGH3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260136, 262 interactors
STRINGi316385.ECDH10B_1410

Protein family/group databases

TCDBi3.A.1.5.42 the atp-binding cassette (abc) superfamily

Proteomic databases

PaxDbiP0AGH3
PRIDEiP0AGH3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74375; AAC74375; b1293
BAA14854; BAA14854; BAA14854
GeneIDi946191
KEGGiecj:JW1286
eco:b1293
PATRICifig|1411691.4.peg.986

Organism-specific databases

EchoBASEiEB4156
EcoGeneiEG20255 sapB

Phylogenomic databases

eggNOGiENOG4108I5W Bacteria
COG4168 LUCA
HOGENOMiHOG000170242
InParanoidiP0AGH3
KOiK19227
PhylomeDBiP0AGH3

Enzyme and pathway databases

BioCyciEcoCyc:SAPB-MONOMER
MetaCyc:SAPB-MONOMER

Miscellaneous databases

PROiPR:P0AGH3

Family and domain databases

CDDicd06261 TM_PBP2, 1 hit
InterProiView protein in InterPro
IPR000515 MetI-like
IPR035906 MetI-like_sf
PfamiView protein in Pfam
PF00528 BPD_transp_1, 1 hit
SUPFAMiSSF161098 SSF161098, 1 hit
PROSITEiView protein in PROSITE
PS50928 ABC_TM1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSAPB_ECOLI
AccessioniPrimary (citable) accession number: P0AGH3
Secondary accession number(s): P76838, Q47623
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 7, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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