sapB

Functionⁱ

Part of a putrescine export transport system, does not play a role in resistance to antimicrobial peptides.1 Publication

GO - Molecular functionⁱ

dipeptide transmembrane transporter activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
putrescine transmembrane transporter activity
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 27803167

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

putrescine transport
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 27803167

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Transport

Biological process

Transport

Enzyme and pathway databases

BioCycⁱ	EcoCyc:SAPB-MONOMER MetaCyc:SAPB-MONOMER

BioCycⁱ

EcoCyc:SAPB-MONOMER
MetaCyc:SAPB-MONOMER

Protein family/group databases

TCDBⁱ	3.A.1.5.42 the atp-binding cassette (abc) superfamily

TCDBⁱ

3.A.1.5.42 the atp-binding cassette (abc) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Putrescine export system permease protein SapBCurated
Gene namesⁱ	Name:sapB Ordered Locus Names:b1293, JW1286
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG20255 sapB

EcoGeneⁱ

EG20255 sapB

Subcellular locationⁱ

Cell inner membrane
1 Publication
Manual assertion based on experiment inⁱ
- Ref.5
  "Global topology analysis of the Escherichia coli inner membrane proteome."
  Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
  Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION, TOPOLOGY [LARGE SCALE ANALYSIS].
; Multi-pass membrane protein
1 Publication
Manual assertion inferred by curator fromⁱ
- Ref.5
  "Global topology analysis of the Escherichia coli inner membrane proteome."
  Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
  Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION, TOPOLOGY [LARGE SCALE ANALYSIS].

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 8	CytoplasmicSequence analysis	8
Transmembraneⁱ	9 – 29	HelicalPROSITE-ProRule annotationAdd BLAST	21
Topological domainⁱ	30 – 80	PeriplasmicSequence analysisAdd BLAST	51
Transmembraneⁱ	81 – 101	HelicalPROSITE-ProRule annotationAdd BLAST	21
Topological domainⁱ	102 – 112	CytoplasmicSequence analysisAdd BLAST	11
Transmembraneⁱ	113 – 133	HelicalPROSITE-ProRule annotationAdd BLAST	21
Topological domainⁱ	134 – 174	PeriplasmicSequence analysisAdd BLAST	41
Transmembraneⁱ	175 – 195	HelicalPROSITE-ProRule annotationAdd BLAST	21
Topological domainⁱ	196 – 248	CytoplasmicSequence analysisAdd BLAST	53
Transmembraneⁱ	249 – 269	HelicalPROSITE-ProRule annotationAdd BLAST	21
Topological domainⁱ	270 – 280	PeriplasmicSequence analysisAdd BLAST	11
Transmembraneⁱ	281 – 301	HelicalPROSITE-ProRule annotationAdd BLAST	21
Topological domainⁱ	302 – 321	CytoplasmicSequence analysis1 PublicationAdd BLAST	20

GO - Cellular componentⁱ

integral component of membrane Source: UniProtKB-KW
plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 15919996

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Single mutation, decreased extracellular putrescine, in an sapBCDF operon deletion no change in resistance to antimicrobial peptide LL-37. Operon deletion has no effect on growth, but if the putrescine importer PuuP is also deleted then growth is slower.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000060158	1 – 321	Putrescine export system permease protein SapBAdd BLAST		321

Proteomic databases

PaxDbⁱ	P0AGH3
PRIDEⁱ	P0AGH3

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4260136, 262 interactors
STRINGⁱ	316385.ECDH10B_1410

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0AGH3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	74 – 302	ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST		229

Sequence similaritiesⁱ

Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4108I5W Bacteria COG4168 LUCA
HOGENOMⁱ	HOG000170242
InParanoidⁱ	P0AGH3
KEGG Orthology (KO) More...KOⁱ	K19227
PhylomeDBⁱ	P0AGH3

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06261 TM_PBP2, 1 hit
InterProⁱ	View protein in InterPro IPR000515 MetI-like IPR035906 MetI-like_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00528 BPD_transp_1, 1 hit
SUPFAMⁱ	SSF161098 SSF161098, 1 hit
PROSITEⁱ	View protein in PROSITE PS50928 ABC_TM1, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AGH3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIIFTLRRIL LLIVTLFLLT FVGFSLSYFT PHAPLQGASL WNAWVFWFNG 
        60         70         80         90        100
LIHWDFGVSS INGQPIAEQL KEVFPATMEL CILAFGFALI VGIPVGMIAG 
       110        120        130        140        150
ITRHKWQDNL INAIALLGFS IPVFWLALLL TLFCSLTLGW LPVSGRFDLL 
       160        170        180        190        200
YEVKPITGFA LIDAWLSDSP WRDEMIMSAI RHMILPVITL SVAPTTEVIR 
       210        220        230        240        250
LMRISTIEVY DQNYVKAAAT RGLSRFTILR RHVLHNALPP VIPRLGLQFS 
       260        270        280        290        300
TMLTLAMITE MVFSWPGLGR WLINAIRQQD YAAISAGVMV CGSLVIIVNV 
       310        320 
ISDILGAMAN PLKHKEWYAL R

Length:321

Mass (Da):36,038

Last modified:December 20, 2005 - v1

Checksum:ⁱE50607A4A2C551F2

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X97282 Genomic DNA Translation: CAA65938.1 U00096 Genomic DNA Translation: AAC74375.1 AP009048 Genomic DNA Translation: BAA14854.1
PIRⁱ	H64877
NCBI Reference Sequences More...RefSeqⁱ	NP_415809.1, NC_000913.3 WP_000583277.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74375; AAC74375; b1293 BAA14854; BAA14854; BAA14854
GeneIDⁱ	946191
KEGGⁱ	ecj:JW1286 eco:b1293
PATRICⁱ	fig\|1411691.4.peg.986

Protein	Similar proteins		Species	Score	Length	Source
P0AGH3	Peptide transport system permease protein SapB		ECO57		321	UniRef100_P0AGH4
Peptide ABC transporter permease		Shigella sp.		321
Peptide ABC transporter permease		Escherichia coli O145 str. RM9872		321
Peptide transport system permease protein SapB		Klebsiella pneumoniae IS22		321
Peptide transport system permease protein sapB		KLEPN		321
+117

Protein	Similar proteins		Species	Score	Length	Source
P0AGH3	Peptide transport system permease protein SapB		ECO57		321	UniRef90_P0AGH4
Peptide ABC transporter permease		Shigella sp.		321
Peptide ABC transporter permease		Escherichia coli O145 str. RM9872		321
Peptide transport system permease protein SapB		Klebsiella pneumoniae IS22		321
Peptide transport system permease protein sapB		KLEPN		321
+722

Protein	Similar proteins		Species	Score	Length	Source
P0AGH3	Peptide transport system permease protein SapB		ECO57		321	UniRef50_P0AGH4
Peptide transport system permease protein SapB		SALTI		321
Peptide transport system permease protein SapB		SALTY		321
Peptide ABC transporter permease		Shigella sp.		321
Peptide ABC transporter permease		Escherichia coli O145 str. RM9872		321
+1905

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X97282 Genomic DNA Translation: CAA65938.1 U00096 Genomic DNA Translation: AAC74375.1 AP009048 Genomic DNA Translation: BAA14854.1
PIRⁱ	H64877
RefSeqⁱ	NP_415809.1, NC_000913.3 WP_000583277.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P0AGH3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260136, 262 interactors
STRINGⁱ	316385.ECDH10B_1410

Protein family/group databases

TCDBⁱ	3.A.1.5.42 the atp-binding cassette (abc) superfamily

TCDBⁱ

3.A.1.5.42 the atp-binding cassette (abc) superfamily

Proteomic databases

PaxDbⁱ	P0AGH3
PRIDEⁱ	P0AGH3

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74375; AAC74375; b1293 BAA14854; BAA14854; BAA14854
GeneIDⁱ	946191
KEGGⁱ	ecj:JW1286 eco:b1293
PATRICⁱ	fig\|1411691.4.peg.986

Organism-specific databases

EchoBASEⁱ	EB4156
EcoGeneⁱ	EG20255 sapB

Phylogenomic databases

eggNOGⁱ	ENOG4108I5W Bacteria COG4168 LUCA
HOGENOMⁱ	HOG000170242
InParanoidⁱ	P0AGH3
KOⁱ	K19227
PhylomeDBⁱ	P0AGH3

Enzyme and pathway databases

BioCycⁱ	EcoCyc:SAPB-MONOMER MetaCyc:SAPB-MONOMER

BioCycⁱ

EcoCyc:SAPB-MONOMER
MetaCyc:SAPB-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0AGH3

PROⁱ

PR:P0AGH3

Family and domain databases

CDDⁱ	cd06261 TM_PBP2, 1 hit
InterProⁱ	View protein in InterPro IPR000515 MetI-like IPR035906 MetI-like_sf
Pfamⁱ	View protein in Pfam PF00528 BPD_transp_1, 1 hit
SUPFAMⁱ	SSF161098 SSF161098, 1 hit
PROSITEⁱ	View protein in PROSITE PS50928 ABC_TM1, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SAPB_ECOLI
Accessionⁱ	P0AGH3Primary (citable) accession number: P0AGH3 Secondary accession number(s): P76838, Q47623
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
	Last sequence update:	December 20, 2005
	Last modified:	November 7, 2018
	This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0AGH3 (SAPB_ECOLI)

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Putrescine export system permease protein SapB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ