UniProtKB - P0AGH3 (SAPB_ECOLI)
Protein
Putrescine export system permease protein SapB
Gene
sapB
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of a putrescine export transport system, does not play a role in resistance to antimicrobial peptides.1 Publication
GO - Molecular functioni
- dipeptide transmembrane transporter activity Source: GO_Central
- putrescine transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- putrescine transport Source: EcoCyc
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:SAPB-MONOMER MetaCyc:SAPB-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.5.42, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Putrescine export system permease protein SapBCurated |
Gene namesi | Name:sapB Ordered Locus Names:b1293, JW1286 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 9 – 29 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 30 – 80 | PeriplasmicSequence analysisAdd BLAST | 51 | |
Transmembranei | 81 – 101 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 102 – 112 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 113 – 133 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 134 – 174 | PeriplasmicSequence analysisAdd BLAST | 41 | |
Transmembranei | 175 – 195 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 196 – 248 | CytoplasmicSequence analysisAdd BLAST | 53 | |
Transmembranei | 249 – 269 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 270 – 280 | PeriplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 281 – 301 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 302 – 321 | CytoplasmicSequence analysis1 PublicationAdd BLAST | 20 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Single mutation, decreased extracellular putrescine, in an sapBCDF operon deletion no change in resistance to antimicrobial peptide LL-37. Operon deletion has no effect on growth, but if the putrescine importer PuuP is also deleted then growth is slower.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060158 | 1 – 321 | Putrescine export system permease protein SapBAdd BLAST | 321 |
Proteomic databases
jPOSTi | P0AGH3 |
PaxDbi | P0AGH3 |
PRIDEi | P0AGH3 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260136, 262 interactors |
ComplexPortali | CPX-4422, Putative peptide ABC transporter |
STRINGi | 511145.b1293 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 74 – 302 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 229 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG4168, Bacteria |
HOGENOMi | CLU_036879_0_3_6 |
InParanoidi | P0AGH3 |
PhylomeDBi | P0AGH3 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
InterProi | View protein in InterPro IPR000515, MetI-like IPR035906, MetI-like_sf |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AGH3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIIFTLRRIL LLIVTLFLLT FVGFSLSYFT PHAPLQGASL WNAWVFWFNG
60 70 80 90 100
LIHWDFGVSS INGQPIAEQL KEVFPATMEL CILAFGFALI VGIPVGMIAG
110 120 130 140 150
ITRHKWQDNL INAIALLGFS IPVFWLALLL TLFCSLTLGW LPVSGRFDLL
160 170 180 190 200
YEVKPITGFA LIDAWLSDSP WRDEMIMSAI RHMILPVITL SVAPTTEVIR
210 220 230 240 250
LMRISTIEVY DQNYVKAAAT RGLSRFTILR RHVLHNALPP VIPRLGLQFS
260 270 280 290 300
TMLTLAMITE MVFSWPGLGR WLINAIRQQD YAAISAGVMV CGSLVIIVNV
310 320
ISDILGAMAN PLKHKEWYAL R
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97282 Genomic DNA Translation: CAA65938.1 U00096 Genomic DNA Translation: AAC74375.1 AP009048 Genomic DNA Translation: BAA14854.1 |
PIRi | H64877 |
RefSeqi | NP_415809.1, NC_000913.3 WP_000583277.1, NZ_STEB01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAC74375; AAC74375; b1293 BAA14854; BAA14854; BAA14854 |
GeneIDi | 57732063 946191 |
KEGGi | ecj:JW1286 eco:b1293 |
PATRICi | fig|1411691.4.peg.986 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X97282 Genomic DNA Translation: CAA65938.1 U00096 Genomic DNA Translation: AAC74375.1 AP009048 Genomic DNA Translation: BAA14854.1 |
PIRi | H64877 |
RefSeqi | NP_415809.1, NC_000913.3 WP_000583277.1, NZ_STEB01000005.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260136, 262 interactors |
ComplexPortali | CPX-4422, Putative peptide ABC transporter |
STRINGi | 511145.b1293 |
Protein family/group databases
TCDBi | 3.A.1.5.42, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P0AGH3 |
PaxDbi | P0AGH3 |
PRIDEi | P0AGH3 |
Genome annotation databases
EnsemblBacteriai | AAC74375; AAC74375; b1293 BAA14854; BAA14854; BAA14854 |
GeneIDi | 57732063 946191 |
KEGGi | ecj:JW1286 eco:b1293 |
PATRICi | fig|1411691.4.peg.986 |
Organism-specific databases
EchoBASEi | EB4156 |
Phylogenomic databases
eggNOGi | COG4168, Bacteria |
HOGENOMi | CLU_036879_0_3_6 |
InParanoidi | P0AGH3 |
PhylomeDBi | P0AGH3 |
Enzyme and pathway databases
BioCyci | EcoCyc:SAPB-MONOMER MetaCyc:SAPB-MONOMER |
Miscellaneous databases
PROi | PR:P0AGH3 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
InterProi | View protein in InterPro IPR000515, MetI-like IPR035906, MetI-like_sf |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SAPB_ECOLI | |
Accessioni | P0AGH3Primary (citable) accession number: P0AGH3 Secondary accession number(s): P76838, Q47623 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families