UniProtKB - P0AGF2 (CSDE_ECOLI)
Protein
Sulfur acceptor protein CsdE
Gene
csdE
Organism
Escherichia coli (strain K12)
Status
Functioni
Stimulates the cysteine desulfurase activity of CsdA. Contains a cysteine residue (Cys-61) that acts to accept sulfur liberated via the desulfurase activity of CsdA. May be able to transfer sulfur to TcdA/CsdL. Seems to support the function of TcdA in the generation of cyclic threonylcarbamoyladenosine at position 37 (ct6A37) in tRNAs that read codons beginning with adenine. Does not appear to participate in Fe/S biogenesis.3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 61 | Cysteine persulfide intermediate | 1 |
GO - Molecular functioni
- sulfur carrier activity Source: EcoCyc
GO - Biological processi
- cyclic threonylcarbamoyladenosine biosynthetic process Source: EcoCyc
Enzyme and pathway databases
BioCyci | EcoCyc:G7455-MONOMER MetaCyc:G7455-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfur acceptor protein CsdE |
Gene namesi | Name:csdE Synonyms:ygdK Ordered Locus Names:b2811, JW2782 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene display a high decrease in the level of ct6A modification in tRNAs, and show the t6A modification instead. They also show no defects in motility, fitness or antibiotic sensitivity, in contrast to csdA mutants.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 61 | C → S: Unable to exert a stimulatory effect on the cysteine desulfurase activity of CsdA and to accept sulfur. Retains the ability to interact with CsdA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202136 | 1 – 147 | Sulfur acceptor protein CsdEAdd BLAST | 147 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 61 | Cysteine persulfide1 Publication | 1 |
Proteomic databases
jPOSTi | P0AGF2 |
PaxDbi | P0AGF2 |
PRIDEi | P0AGF2 |
Interactioni
Subunit structurei
Homodimer.
Forms a heterodimer with CsdA.
Interacts with CsdA and with TcdA/CsdL.
2 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4261123, 398 interactors 851604, 1 interactor |
ComplexPortali | CPX-2138, CdsA-CdsE complex |
DIPi | DIP-12143N |
IntActi | P0AGF2, 5 interactors |
STRINGi | 511145.b2811 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0AGF2 |
SMRi | P0AGF2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AGF2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the SufE family.Curated
Phylogenomic databases
eggNOGi | COG2166, Bacteria |
HOGENOMi | CLU_124502_1_0_6 |
InParanoidi | P0AGF2 |
PhylomeDBi | P0AGF2 |
Family and domain databases
InterProi | View protein in InterPro IPR017763, Cysteine_desulfurase_CsdE IPR003808, Fe-S_metab-assoc_dom |
PANTHERi | PTHR43597, PTHR43597, 1 hit |
Pfami | View protein in Pfam PF02657, SufE, 1 hit |
TIGRFAMsi | TIGR03391, FeS_syn_CsdE, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AGF2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTNPQFAGHP FGTTVTAETL RNTFAPLTQW EDKYRQLIML GKQLPALPDE
60 70 80 90 100
LKAQAKEIAG CENRVWLGYT VAENGKMHFF GDSEGRIVRG LLAVLLTAVE
110 120 130 140
GKTAAELQAQ SPLALFDELG LRAQLSASRS QGLNALSEAI IAATKQV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29581 Genomic DNA Translation: AAB40461.1 U00096 Genomic DNA Translation: AAC75853.1 AP009048 Genomic DNA Translation: BAE76883.1 |
PIRi | G65063 |
RefSeqi | NP_417291.1, NC_000913.3 WP_000184261.1, NZ_SSUV01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC75853; AAC75853; b2811 BAE76883; BAE76883; BAE76883 |
GeneIDi | 57730385 947274 |
KEGGi | ecj:JW2782 eco:b2811 |
PATRICi | fig|1411691.4.peg.3922 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29581 Genomic DNA Translation: AAB40461.1 U00096 Genomic DNA Translation: AAC75853.1 AP009048 Genomic DNA Translation: BAE76883.1 |
PIRi | G65063 |
RefSeqi | NP_417291.1, NC_000913.3 WP_000184261.1, NZ_SSUV01000026.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NI7 | NMR | - | A | 1-147 | [»] | |
5EEP | X-ray | 2.40 | A | 1-147 | [»] | |
5FT8 | X-ray | 2.50 | B/D/F/H/J/L/N/P | 1-147 | [»] | |
5NQ6 | X-ray | 2.40 | A/B | 1-147 | [»] | |
BMRBi | P0AGF2 | |||||
SMRi | P0AGF2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261123, 398 interactors 851604, 1 interactor |
ComplexPortali | CPX-2138, CdsA-CdsE complex |
DIPi | DIP-12143N |
IntActi | P0AGF2, 5 interactors |
STRINGi | 511145.b2811 |
Proteomic databases
jPOSTi | P0AGF2 |
PaxDbi | P0AGF2 |
PRIDEi | P0AGF2 |
Genome annotation databases
EnsemblBacteriai | AAC75853; AAC75853; b2811 BAE76883; BAE76883; BAE76883 |
GeneIDi | 57730385 947274 |
KEGGi | ecj:JW2782 eco:b2811 |
PATRICi | fig|1411691.4.peg.3922 |
Organism-specific databases
EchoBASEi | EB2892 |
Phylogenomic databases
eggNOGi | COG2166, Bacteria |
HOGENOMi | CLU_124502_1_0_6 |
InParanoidi | P0AGF2 |
PhylomeDBi | P0AGF2 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7455-MONOMER MetaCyc:G7455-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AGF2 |
PROi | PR:P0AGF2 |
Family and domain databases
InterProi | View protein in InterPro IPR017763, Cysteine_desulfurase_CsdE IPR003808, Fe-S_metab-assoc_dom |
PANTHERi | PTHR43597, PTHR43597, 1 hit |
Pfami | View protein in Pfam PF02657, SufE, 1 hit |
TIGRFAMsi | TIGR03391, FeS_syn_CsdE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CSDE_ECOLI | |
Accessioni | P0AGF2Primary (citable) accession number: P0AGF2 Secondary accession number(s): Q2MA23, Q46926 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families