UniProtKB - P0AGE4 (SSTT_ECOLI)
Protein
Serine/threonine transporter SstT
Gene
sstT
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the import of serine and threonine into the cell, with the concomitant import of sodium (symport system).2 Publications
Activity regulationi
Activated by valinomycin and inhibited by monensin.1 Publication
Kineticsi
- KM=0.82 µM for serine1 Publication
- Vmax=0.37 µmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 7.0.1 Publication
Temperature dependencei
Optimum temperature is 40 degrees Celsius.1 Publication
GO - Molecular functioni
- neutral amino acid:sodium symporter activity Source: EcoliWiki
- neutral amino acid transmembrane transporter activity Source: EcoliWiki
- transmembrane transporter activity Source: GO_Central
GO - Biological processi
- amino acid transport Source: EcoliWiki
- serine transport Source: EcoliWiki
- threonine transport Source: InterPro
Keywordsi
Biological process | Amino-acid transport, Symport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:YGJU-MONOMER ECOL316407:JW3060-MONOMER MetaCyc:YGJU-MONOMER |
Protein family/group databases
TCDBi | 2.A.23.4.1 the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine transporter SstTAlternative name(s): Na(+)/serine-threonine symporter |
Gene namesi | Name:sstT Synonyms:ygjU Ordered Locus Names:b3089, JW3060 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 15 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 16 – 36 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 37 – 45 | PeriplasmicSequence analysis | 9 | |
Transmembranei | 46 – 66 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 67 – 83 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 84 – 104 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 105 – 142 | PeriplasmicSequence analysisAdd BLAST | 38 | |
Transmembranei | 143 – 163 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 164 – 179 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 180 – 200 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 201 – 217 | PeriplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 218 – 238 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 239 – 299 | CytoplasmicSequence analysisAdd BLAST | 61 | |
Transmembranei | 300 – 320 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 321 – 331 | PeriplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 332 – 352 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 353 – 414 | CytoplasmicSequence analysisAdd BLAST | 62 |
GO - Cellular componenti
- integral component of membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000202118 | 2 – 414 | Serine/threonine transporter SstTAdd BLAST | 413 |
Proteomic databases
EPDi | P0AGE4 |
PaxDbi | P0AGE4 |
PRIDEi | P0AGE4 |
Expressioni
Inductioni
Repressed by tryptophan.1 Publication
Interactioni
Protein-protein interaction databases
BioGridi | 4260951, 17 interactors |
STRINGi | 511145.b3089 |
Family & Domainsi
Sequence similaritiesi
Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG4107QV7 Bacteria COG3633 LUCA |
HOGENOMi | HOG000218252 |
InParanoidi | P0AGE4 |
KOi | K07862 |
PhylomeDBi | P0AGE4 |
Family and domain databases
Gene3Di | 1.10.3860.10, 1 hit |
HAMAPi | MF_01582 Ser_Thr_transp_SstT, 1 hit |
InterProi | View protein in InterPro IPR001991 Na-dicarboxylate_symporter IPR036458 Na:dicarbo_symporter_sf IPR023025 Ser_Thr_transp_SstT |
Pfami | View protein in Pfam PF00375 SDF, 1 hit |
SUPFAMi | SSF118215 SSF118215, 1 hit |
PROSITEi | View protein in PROSITE PS00713 NA_DICARBOXYL_SYMP_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AGE4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTQRSPGLF RRLAHGSLVK QILVGLVLGI LLAWISKPAA EAVGLLGTLF
60 70 80 90 100
VGALKAVAPI LVLMLVMASI ANHQHGQKTN IRPILFLYLL GTFSAALAAV
110 120 130 140 150
VFSFAFPSTL HLSSSAGDIS PPSGIVEVMR GLVMSMVSNP IDALLKGNYI
160 170 180 190 200
GILVWAIGLG FALRHGNETT KNLVNDMSNA VTFMVKLVIR FAPIGIFGLV
210 220 230 240 250
SSTLATTGFS TLWGYAQLLV VLVGCMLLVA LVVNPLLVWW KIRRNPFPLV
260 270 280 290 300
LLCLRESGVY AFFTRSSAAN IPVNMALCEK LNLDRDTYSV SIPLGATINM
310 320 330 340 350
AGAAITITVL TLAAVNTLGI PVDLPTALLL SVVASLCACG ASGVAGGSLL
360 370 380 390 400
LIPLACNMFG ISNDIAMQVV AVGFIIGVLQ DSCETALNSS TDVLFTAAAC
410
QAEDDRLANS ALRN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57891.1 U00096 Genomic DNA Translation: AAC76124.1 AP009048 Genomic DNA Translation: BAE77139.1 D13328 Genomic DNA No translation available. |
PIRi | F65097 |
RefSeqi | NP_417560.1, NC_000913.3 WP_000211655.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC76124; AAC76124; b3089 BAE77139; BAE77139; BAE77139 |
GeneIDi | 947605 |
KEGGi | ecj:JW3060 eco:b3089 |
PATRICi | fig|1411691.4.peg.3640 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57891.1 U00096 Genomic DNA Translation: AAC76124.1 AP009048 Genomic DNA Translation: BAE77139.1 D13328 Genomic DNA No translation available. |
PIRi | F65097 |
RefSeqi | NP_417560.1, NC_000913.3 WP_000211655.1, NZ_STEB01000001.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGridi | 4260951, 17 interactors |
STRINGi | 511145.b3089 |
Protein family/group databases
TCDBi | 2.A.23.4.1 the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family |
Proteomic databases
EPDi | P0AGE4 |
PaxDbi | P0AGE4 |
PRIDEi | P0AGE4 |
Genome annotation databases
EnsemblBacteriai | AAC76124; AAC76124; b3089 BAE77139; BAE77139; BAE77139 |
GeneIDi | 947605 |
KEGGi | ecj:JW3060 eco:b3089 |
PATRICi | fig|1411691.4.peg.3640 |
Organism-specific databases
EchoBASEi | EB2590 |
Phylogenomic databases
eggNOGi | ENOG4107QV7 Bacteria COG3633 LUCA |
HOGENOMi | HOG000218252 |
InParanoidi | P0AGE4 |
KOi | K07862 |
PhylomeDBi | P0AGE4 |
Enzyme and pathway databases
BioCyci | EcoCyc:YGJU-MONOMER ECOL316407:JW3060-MONOMER MetaCyc:YGJU-MONOMER |
Miscellaneous databases
PROi | PR:P0AGE4 |
Family and domain databases
Gene3Di | 1.10.3860.10, 1 hit |
HAMAPi | MF_01582 Ser_Thr_transp_SstT, 1 hit |
InterProi | View protein in InterPro IPR001991 Na-dicarboxylate_symporter IPR036458 Na:dicarbo_symporter_sf IPR023025 Ser_Thr_transp_SstT |
Pfami | View protein in Pfam PF00375 SDF, 1 hit |
SUPFAMi | SSF118215 SSF118215, 1 hit |
PROSITEi | View protein in PROSITE PS00713 NA_DICARBOXYL_SYMP_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SSTT_ECOLI | |
Accessioni | P0AGE4Primary (citable) accession number: P0AGE4 Secondary accession number(s): P42602, Q2M9B7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | November 13, 2019 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene