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Protein

Hexose-6-phosphate:phosphate antiporter

Gene

uhpT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the exchange of external hexose 6-phosphate and internal inorganic phosphate. Can transport glucose-6-phosphate, fructose-6-phosphate and mannose-6-phosphate. Also catalyzes the neutral exchange of internal and external phosphate.6 Publications

Enzyme regulationi

Reaction is unaffected by the ionophores valinomycin, valinomycin plus nigericin, and carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP).1 Publication

GO - Molecular functioni

  • hexose-phosphate:inorganic phosphate antiporter activity Source: EcoCyc

GO - Biological processi

  • carbohydrate transport Source: UniProtKB-KW
  • glucose-6-phosphate transport Source: EcoCyc
  • hexose phosphate transport Source: EcoCyc

Keywordsi

Biological processPhosphate transport, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:UHPT-MONOMER
MetaCyc:UHPT-MONOMER

Protein family/group databases

TCDBi2.A.1.4.1 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Hexose-6-phosphate:phosphate antiporterCurated
Gene namesi
Name:uhpT
Ordered Locus Names:b3666, JW3641
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11054 uhpT

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24Cytoplasmic1 PublicationAdd BLAST24
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Topological domaini46 – 60Periplasmic1 PublicationAdd BLAST15
Transmembranei61 – 81HelicalSequence analysisAdd BLAST21
Topological domaini82 – 96Cytoplasmic1 PublicationAdd BLAST15
Transmembranei97 – 117HelicalSequence analysisAdd BLAST21
Topological domaini118 – 120Periplasmic1 Publication3
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Topological domaini142 – 159Cytoplasmic2 PublicationsAdd BLAST18
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 189Periplasmic1 Publication9
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 259Cytoplasmic1 PublicationAdd BLAST49
Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
Topological domaini281 – 297Periplasmic1 PublicationAdd BLAST17
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Topological domaini319 – 326Cytoplasmic1 Publication8
Transmembranei327 – 347HelicalSequence analysisAdd BLAST21
Topological domaini348 – 357Periplasmic1 Publication10
Transmembranei358 – 378HelicalSequence analysisAdd BLAST21
Topological domaini379 – 391Cytoplasmic1 PublicationAdd BLAST13
Transmembranei392 – 411HelicalSequence analysisAdd BLAST20
Topological domaini412 – 425Periplasmic1 PublicationAdd BLAST14
Transmembranei426 – 446HelicalSequence analysisAdd BLAST21
Topological domaini447 – 463Cytoplasmic1 Publication1 PublicationAdd BLAST17

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi108C → S: No change in activity. 1 Publication1
Mutagenesisi143C → S: 30% of wild-type sugar phosphate transport activity. 1 Publication1
Mutagenesisi265C → S: No change in activity. 1 Publication1
Mutagenesisi331C → S: No change in activity. 1 Publication1
Mutagenesisi436C → S: No change in activity. 1 Publication1
Mutagenesisi438C → S: No change in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001998831 – 463Hexose-6-phosphate:phosphate antiporterAdd BLAST463

Proteomic databases

PaxDbiP0AGC0
PRIDEiP0AGC0

Expressioni

Inductioni

Expression is induced by external glucose-6-phosphate through the UhpABC signaling cascade.4 Publications

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262581, 5 interactors
STRINGi316385.ECDH10B_3849

Structurei

3D structure databases

ProteinModelPortaliP0AGC0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CXY Bacteria
COG2271 LUCA
HOGENOMiHOG000274728
InParanoidiP0AGC0
KOiK07784
OMAiTKWTPRN
PhylomeDBiP0AGC0

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
IPR021159 Sugar-P_transporter_CS
IPR000849 Sugar_P_transporter
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
PIRSFiPIRSF002808 Hexose_phosphate_transp, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
PROSITEiView protein in PROSITE
PS00942 GLPT, 1 hit
PS50850 MFS, 1 hit

Sequencei

Sequence statusi: Complete.

P0AGC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAFLNQVRK PTLDLPLEVR RKMWFKPFMQ SYLVVFIGYL TMYLIRKNFN
60 70 80 90 100
IAQNDMISTY GLSMTQLGMI GLGFSITYGV GKTLVSYYAD GKNTKQFLPF
110 120 130 140 150
MLILSAICML GFSASMGSGS VSLFLMIAFY ALSGFFQSTG GSCSYSTITK
160 170 180 190 200
WTPRRKRGTF LGFWNISHNL GGAGAAGVAL FGANYLFDGH VIGMFIFPSI
210 220 230 240 250
IALIVGFIGL RYGSDSPESY GLGKAEELFG EEISEEDKET ESTDMTKWQI
260 270 280 290 300
FVEYVLKNKV IWLLCFANIF LYVVRIGIDQ WSTVYAFQEL KLSKAVAIQG
310 320 330 340 350
FTLFEAGALV GTLLWGWLSD LANGRRGLVA CIALALIIAT LGVYQHASNE
360 370 380 390 400
YIYLASLFAL GFLVFGPQLL IGVAAVGFVP KKAIGAADGI KGTFAYLIGD
410 420 430 440 450
SFAKLGLGMI ADGTPVFGLT GWAGTFAALD IAAIGCICLM AIVAVMEERK
460
IRREKKIQQL TVA
Length:463
Mass (Da):50,607
Last modified:December 20, 2005 - v1
Checksum:i1BE23CEFF05311B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA Translation: AAA24723.1
M89479 Genomic DNA Translation: AAA24727.1
L10328 Genomic DNA Translation: AAA62018.1
U00096 Genomic DNA Translation: AAC76689.1
AP009048 Genomic DNA Translation: BAE77627.1
PIRiA30395 MMECHP
RefSeqiNP_418122.1, NC_000913.3
WP_000879194.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76689; AAC76689; b3666
BAE77627; BAE77627; BAE77627
GeneIDi948201
KEGGiecj:JW3641
eco:b3666
PATRICifig|1411691.4.peg.3039

Similar proteinsi

Entry informationi

Entry nameiUHPT_ECOLI
AccessioniPrimary (citable) accession number: P0AGC0
Secondary accession number(s): P13408, P76727, Q2M7X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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