UniProtKB - P0AGC0 (UHPT_ECOLI)
Protein
Hexose-6-phosphate:phosphate antiporter
Gene
uhpT
Organism
Escherichia coli (strain K12)
Status
Functioni
Mediates the exchange of external hexose 6-phosphate and internal inorganic phosphate. Can transport glucose-6-phosphate, fructose-6-phosphate and mannose-6-phosphate. Also catalyzes the neutral exchange of internal and external phosphate.6 Publications
Activity regulationi
Reaction is unaffected by the ionophores valinomycin, valinomycin plus nigericin, and carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP).1 Publication
GO - Molecular functioni
- hexose-phosphate:inorganic phosphate antiporter activity Source: EcoCyc
GO - Biological processi
- carbohydrate transport Source: UniProtKB-KW
- glucose-6-phosphate transport Source: EcoCyc
- hexose phosphate transport Source: EcoCyc
Keywordsi
Biological process | Phosphate transport, Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:UHPT-MONOMER MetaCyc:UHPT-MONOMER |
Protein family/group databases
TCDBi | 2.A.1.4.1, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Hexose-6-phosphate:phosphate antiporterCurated |
Gene namesi | Name:uhpT Ordered Locus Names:b3666, JW3641 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 3 Publications; Multi-pass membrane protein Sequence analysis1 Publication
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 24 | Cytoplasmic1 PublicationAdd BLAST | 24 | |
Transmembranei | 25 – 45 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 46 – 60 | Periplasmic1 PublicationAdd BLAST | 15 | |
Transmembranei | 61 – 81 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 82 – 96 | Cytoplasmic1 PublicationAdd BLAST | 15 | |
Transmembranei | 97 – 117 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 118 – 120 | Periplasmic1 Publication | 3 | |
Transmembranei | 121 – 141 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 142 – 159 | Cytoplasmic2 PublicationsAdd BLAST | 18 | |
Transmembranei | 160 – 180 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 181 – 189 | Periplasmic1 Publication | 9 | |
Transmembranei | 190 – 210 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 211 – 259 | Cytoplasmic1 PublicationAdd BLAST | 49 | |
Transmembranei | 260 – 280 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 281 – 297 | Periplasmic1 PublicationAdd BLAST | 17 | |
Transmembranei | 298 – 318 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 319 – 326 | Cytoplasmic1 Publication | 8 | |
Transmembranei | 327 – 347 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 348 – 357 | Periplasmic1 Publication | 10 | |
Transmembranei | 358 – 378 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 379 – 391 | Cytoplasmic1 PublicationAdd BLAST | 13 | |
Transmembranei | 392 – 411 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 412 – 425 | Periplasmic1 PublicationAdd BLAST | 14 | |
Transmembranei | 426 – 446 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 447 – 463 | Cytoplasmic1 Publication1 PublicationAdd BLAST | 17 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 108 | C → S: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 143 | C → S: 30% of wild-type sugar phosphate transport activity. 1 Publication | 1 | |
Mutagenesisi | 265 | C → S: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 331 | C → S: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 436 | C → S: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 438 | C → S: No change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199883 | 1 – 463 | Hexose-6-phosphate:phosphate antiporterAdd BLAST | 463 |
Proteomic databases
PaxDbi | P0AGC0 |
PRIDEi | P0AGC0 |
Expressioni
Inductioni
Expression is induced by external glucose-6-phosphate through the UhpABC signaling cascade.4 Publications
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262581, 5 interactors |
STRINGi | 511145.b3666 |
Family & Domainsi
Sequence similaritiesi
Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2271, Bacteria |
HOGENOMi | CLU_001265_31_0_6 |
InParanoidi | P0AGC0 |
PhylomeDBi | P0AGC0 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR021159, Sugar-P_transporter_CS IPR000849, Sugar_P_transporter |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
PIRSFi | PIRSF002808, Hexose_phosphate_transp, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS00942, GLPT, 1 hit PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AGC0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLAFLNQVRK PTLDLPLEVR RKMWFKPFMQ SYLVVFIGYL TMYLIRKNFN
60 70 80 90 100
IAQNDMISTY GLSMTQLGMI GLGFSITYGV GKTLVSYYAD GKNTKQFLPF
110 120 130 140 150
MLILSAICML GFSASMGSGS VSLFLMIAFY ALSGFFQSTG GSCSYSTITK
160 170 180 190 200
WTPRRKRGTF LGFWNISHNL GGAGAAGVAL FGANYLFDGH VIGMFIFPSI
210 220 230 240 250
IALIVGFIGL RYGSDSPESY GLGKAEELFG EEISEEDKET ESTDMTKWQI
260 270 280 290 300
FVEYVLKNKV IWLLCFANIF LYVVRIGIDQ WSTVYAFQEL KLSKAVAIQG
310 320 330 340 350
FTLFEAGALV GTLLWGWLSD LANGRRGLVA CIALALIIAT LGVYQHASNE
360 370 380 390 400
YIYLASLFAL GFLVFGPQLL IGVAAVGFVP KKAIGAADGI KGTFAYLIGD
410 420 430 440 450
SFAKLGLGMI ADGTPVFGLT GWAGTFAALD IAAIGCICLM AIVAVMEERK
460
IRREKKIQQL TVA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17102 Genomic DNA Translation: AAA24723.1 M89479 Genomic DNA Translation: AAA24727.1 L10328 Genomic DNA Translation: AAA62018.1 U00096 Genomic DNA Translation: AAC76689.1 AP009048 Genomic DNA Translation: BAE77627.1 |
PIRi | A30395, MMECHP |
RefSeqi | NP_418122.1, NC_000913.3 WP_000879194.1, NZ_STEB01000015.1 |
Genome annotation databases
EnsemblBacteriai | AAC76689; AAC76689; b3666 BAE77627; BAE77627; BAE77627 |
GeneIDi | 58389656 948201 |
KEGGi | ecj:JW3641 eco:b3666 |
PATRICi | fig|1411691.4.peg.3039 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M17102 Genomic DNA Translation: AAA24723.1 M89479 Genomic DNA Translation: AAA24727.1 L10328 Genomic DNA Translation: AAA62018.1 U00096 Genomic DNA Translation: AAC76689.1 AP009048 Genomic DNA Translation: BAE77627.1 |
PIRi | A30395, MMECHP |
RefSeqi | NP_418122.1, NC_000913.3 WP_000879194.1, NZ_STEB01000015.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262581, 5 interactors |
STRINGi | 511145.b3666 |
Protein family/group databases
TCDBi | 2.A.1.4.1, the major facilitator superfamily (mfs) |
Proteomic databases
PaxDbi | P0AGC0 |
PRIDEi | P0AGC0 |
Genome annotation databases
EnsemblBacteriai | AAC76689; AAC76689; b3666 BAE77627; BAE77627; BAE77627 |
GeneIDi | 58389656 948201 |
KEGGi | ecj:JW3641 eco:b3666 |
PATRICi | fig|1411691.4.peg.3039 |
Organism-specific databases
EchoBASEi | EB1047 |
Phylogenomic databases
eggNOGi | COG2271, Bacteria |
HOGENOMi | CLU_001265_31_0_6 |
InParanoidi | P0AGC0 |
PhylomeDBi | P0AGC0 |
Enzyme and pathway databases
BioCyci | EcoCyc:UHPT-MONOMER MetaCyc:UHPT-MONOMER |
Miscellaneous databases
PROi | PR:P0AGC0 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR021159, Sugar-P_transporter_CS IPR000849, Sugar_P_transporter |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
PIRSFi | PIRSF002808, Hexose_phosphate_transp, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS00942, GLPT, 1 hit PS50850, MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UHPT_ECOLI | |
Accessioni | P0AGC0Primary (citable) accession number: P0AGC0 Secondary accession number(s): P13408, P76727, Q2M7X9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families