Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoserine phosphatase

Gene

serB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of phosphoserine (P-Ser). Also catalyzes the hydrolysis of phosphothreonine (P-Thr).1 Publication

Catalytic activityi

O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Zn2+1 PublicationNote: Binds 1 Mg2+ ion per subunit. Can also use other divalent metal cations as Mn2+, Co2+ and Zn2+.1 Publication

Kineticsi

  1. KM=0.097 mM for P-Ser (with magnesium ions as cofactor and at pH 9)1 Publication
  2. KM=0.07 mM for imido-diphosphate (with magnesium ions as cofactor and at pH 9)1 Publication
  3. KM=6.2 mM for acetyl-phosphate (with magnesium ions as cofactor and at pH 9)1 Publication

    pH dependencei

    Optimum pH is between 6 and 7.5.1 Publication

    Pathwayi: L-serine biosynthesis

    This protein is involved in step 3 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. D-3-phosphoglycerate dehydrogenase (serA)
    2. Phosphoserine aminotransferase (serC)
    3. Phosphoserine phosphatase (serB)
    This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi12MagnesiumBy similarity1
    Active sitei116NucleophileBy similarity1
    Metal bindingi116MagnesiumBy similarity1
    Active sitei118Proton donorBy similarity1
    Metal bindingi118Magnesium; via carbonyl oxygenBy similarity1
    Binding sitei125SubstrateBy similarity1
    Binding sitei161SubstrateBy similarity1
    Binding sitei249SubstrateBy similarity1
    Metal bindingi272MagnesiumBy similarity1
    Binding sitei275SubstrateBy similarity1

    GO - Molecular functioni

    • magnesium ion binding Source: EcoliWiki
    • phosphatase activity Source: EcoliWiki
    • phosphoserine phosphatase activity Source: EcoliWiki

    GO - Biological processi

    • cellular amino acid biosynthetic process Source: EcoliWiki
    • dephosphorylation Source: GO_Central
    • L-serine biosynthetic process Source: EcoliWiki

    Keywordsi

    Molecular functionHydrolase
    Biological processAmino-acid biosynthesis, Serine biosynthesis
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PSERPHOSPHA-MONOMER
    MetaCyc:PSERPHOSPHA-MONOMER
    SABIO-RKiP0AGB0
    UniPathwayi
    UPA00135;UER00198

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoserine phosphatase (EC:3.1.3.3)
    Short name:
    PSP
    Short name:
    PSPase
    Alternative name(s):
    O-phosphoserine phosphohydrolase
    Gene namesi
    Name:serB
    Ordered Locus Names:b4388, JW4351
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10945 serB

    Subcellular locationi

    GO - Cellular componenti

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001568861 – 322Phosphoserine phosphataseAdd BLAST322

    Proteomic databases

    PaxDbiP0AGB0
    PRIDEiP0AGB0

    Interactioni

    Protein-protein interaction databases

    BioGridi4262789, 119 interactors
    DIPiDIP-48100N
    IntActiP0AGB0, 3 interactors
    STRINGi316385.ECDH10B_4546

    Structurei

    3D structure databases

    ProteinModelPortaliP0AGB0
    SMRiP0AGB0
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni10 – 12Substrate bindingBy similarity3
    Regioni204 – 205Substrate bindingBy similarity2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105EAC Bacteria
    COG0560 LUCA
    HOGENOMiHOG000231115
    InParanoidiP0AGB0
    KOiK01079
    PhylomeDBiP0AGB0

    Family and domain databases

    Gene3Di1.10.150.210, 1 hit
    3.40.50.1000, 1 hit
    InterProiView protein in InterPro
    IPR036412 HAD-like_sf
    IPR023214 HAD_sf
    IPR023190 Pser_Pase_dom_2
    SUPFAMiSSF56784 SSF56784, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0AGB0-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MPNITWCDLP EDVSLWPGLP LSLSGDEVMP LDYHAGRSGW LLYGRGLDKQ
    60 70 80 90 100
    RLTQYQSKLG AAMVIVAAWC VEDYQVIRLA GSLTARATRL AHEAQLDVAP
    110 120 130 140 150
    LGKIPHLRTP GLLVMDMDST AIQIECIDEI AKLAGTGEMV AEVTERAMRG
    160 170 180 190 200
    ELDFTASLRS RVATLKGADA NILQQVRENL PLMPGLTQLV LKLETLGWKV
    210 220 230 240 250
    AIASGGFTFF AEYLRDKLRL TAVVANELEI MDGKFTGNVI GDIVDAQYKA
    260 270 280 290 300
    KTLTRLAQEY EIPLAQTVAI GDGANDLPMI KAAGLGIAYH AKPKVNEKAE
    310 320
    VTIRHADLMG VFCILSGSLN QK
    Length:322
    Mass (Da):35,043
    Last modified:January 1, 1988 - v1
    Checksum:iA09A43BD846B7DE6
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X03046 Genomic DNA Translation: CAA26852.1
    U14003 Genomic DNA Translation: AAA97284.1
    U00096 Genomic DNA Translation: AAC77341.1
    AP009048 Genomic DNA Translation: BAE78377.1
    PIRiA24271 PAECS
    RefSeqiNP_418805.1, NC_000913.3
    WP_001132955.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC77341; AAC77341; b4388
    BAE78377; BAE78377; BAE78377
    GeneIDi948913
    KEGGiecj:JW4351
    eco:b4388
    PATRICifig|1411691.4.peg.2297

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X03046 Genomic DNA Translation: CAA26852.1
    U14003 Genomic DNA Translation: AAA97284.1
    U00096 Genomic DNA Translation: AAC77341.1
    AP009048 Genomic DNA Translation: BAE78377.1
    PIRiA24271 PAECS
    RefSeqiNP_418805.1, NC_000913.3
    WP_001132955.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP0AGB0
    SMRiP0AGB0
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262789, 119 interactors
    DIPiDIP-48100N
    IntActiP0AGB0, 3 interactors
    STRINGi316385.ECDH10B_4546

    Proteomic databases

    PaxDbiP0AGB0
    PRIDEiP0AGB0

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC77341; AAC77341; b4388
    BAE78377; BAE78377; BAE78377
    GeneIDi948913
    KEGGiecj:JW4351
    eco:b4388
    PATRICifig|1411691.4.peg.2297

    Organism-specific databases

    EchoBASEiEB0938
    EcoGeneiEG10945 serB

    Phylogenomic databases

    eggNOGiENOG4105EAC Bacteria
    COG0560 LUCA
    HOGENOMiHOG000231115
    InParanoidiP0AGB0
    KOiK01079
    PhylomeDBiP0AGB0

    Enzyme and pathway databases

    UniPathwayi
    UPA00135;UER00198

    BioCyciEcoCyc:PSERPHOSPHA-MONOMER
    MetaCyc:PSERPHOSPHA-MONOMER
    SABIO-RKiP0AGB0

    Miscellaneous databases

    PROiPR:P0AGB0

    Family and domain databases

    Gene3Di1.10.150.210, 1 hit
    3.40.50.1000, 1 hit
    InterProiView protein in InterPro
    IPR036412 HAD-like_sf
    IPR023214 HAD_sf
    IPR023190 Pser_Pase_dom_2
    SUPFAMiSSF56784 SSF56784, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSERB_ECOLI
    AccessioniPrimary (citable) accession number: P0AGB0
    Secondary accession number(s): P06862, Q2M5S9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: January 1, 1988
    Last modified: November 7, 2018
    This is version 103 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again