UniProtKB - P0AGA2 (SECY_ECOLI)
Protein translocase subunit SecY
secY
Functioni
The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently. SecY is required to insert newly synthesized SecY into the inner membrane. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true, overexpression also results in FtsH-mediated degradation of SecY.
GO - Molecular functioni
- protein transmembrane transporter activity Source: EcoCyc
- signal sequence binding Source: EcoCyc
GO - Biological processi
- intracellular protein transmembrane transport Source: EcoCyc
- intracellular protein transport Source: EcoliWiki
- protein insertion into membrane from inner side Source: EcoCyc
- protein transport by the Sec complex Source: ComplexPortal
- SRP-dependent cotranslational protein targeting to membrane, translocation Source: EcoCyc
Keywordsi
Biological process | Protein transport, Translocation, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:SECY |
Protein family/group databases
TCDBi | 3.A.5.1.1, the general secretory pathway (sec) family |
Names & Taxonomyi
Protein namesi | Recommended name: Protein translocase subunit SecY |
Gene namesi | Name:secY Synonyms:prlA Ordered Locus Names:b3300, JW3262 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: CACAO
- plasma membrane Source: EcoCyc
Other locations
- cell envelope Sec protein transport complex Source: EcoCyc
- integral component of membrane Source: EcoliWiki
- membrane Source: ComplexPortal
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | CytoplasmicCuratedAdd BLAST | 22 | |
Transmembranei | 23 – 35 | Helical; Name=1CuratedAdd BLAST | 13 | |
Topological domaini | 36 – 60 | PeriplasmicBy similarityAdd BLAST | 25 | |
Transmembranei | 61 – 96 | Discontinuously helical; Name=2By similarityAdd BLAST | 36 | |
Intramembranei | 61 – 70 | Helical; Name=Helix 2ACurated | 10 | |
Intramembranei | 71 – 76 | Curated | 6 | |
Intramembranei | 77 – 96 | Helical; Name=Helix 2BCuratedAdd BLAST | 20 | |
Topological domaini | 97 – 115 | CytoplasmicCuratedAdd BLAST | 19 | |
Transmembranei | 116 – 131 | Helical; Name=3CuratedAdd BLAST | 16 | |
Topological domaini | 132 – 164 | PeriplasmicCuratedAdd BLAST | 33 | |
Transmembranei | 165 – 178 | Helical; Name=4CuratedAdd BLAST | 14 | |
Topological domaini | 179 – 183 | CytoplasmicCurated | 5 | |
Transmembranei | 184 – 200 | Helical; Name=5CuratedAdd BLAST | 17 | |
Topological domaini | 201 – 223 | PeriplasmicCuratedAdd BLAST | 23 | |
Transmembranei | 224 – 237 | Helical; Name=6CuratedAdd BLAST | 14 | |
Topological domaini | 238 – 273 | CytoplasmicCuratedAdd BLAST | 36 | |
Transmembranei | 274 – 287 | Helical; Name=7CuratedAdd BLAST | 14 | |
Topological domaini | 288 – 313 | PeriplasmicCuratedAdd BLAST | 26 | |
Transmembranei | 314 – 329 | Helical; Name=8CuratedAdd BLAST | 16 | |
Topological domaini | 330 – 380 | CytoplasmicCuratedAdd BLAST | 51 | |
Transmembranei | 381 – 395 | Helical; Name=9CuratedAdd BLAST | 15 | |
Topological domaini | 396 | PeriplasmicCurated | 1 | |
Transmembranei | 397 – 413 | Helical; Name=10CuratedAdd BLAST | 17 | |
Topological domaini | 414 – 443 | CytoplasmicCuratedAdd BLAST | 30 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 40 | P → S in secY100; temperature-sensitive. | 1 | |
Mutagenesisi | 60 – 74 | Missing : Some loss of viability, supports protein translocation; strongly suppresses defective and missing signal sequences; transient transmembrane channels open. 2 PublicationsAdd BLAST | 15 | |
Mutagenesisi | 65 – 70 | Missing : Grows almost as well as wild-type, supports protein translocation; strongly suppresses defective and missing signal sequences; transient transmembrane channels open. 2 Publications | 6 | |
Mutagenesisi | 67 | F → C in prlA3; altered signal sequence interaction, transient channel opening and closing in presence of oxidant; massive ion flux when cross-linked to SecE C-120 mutation. 1 Publication | 1 | |
Mutagenesisi | 167 | G → E in secY100; temperature-sensitive. | 1 | |
Mutagenesisi | 240 | G → D in secY24; temperature-sensitive at 42 degrees Celsius, impairs interaction with SecE even at 30 degrees in vitro. 1 Publication | 1 | |
Mutagenesisi | 282 | S → R in prlA401; altered signal sequence interaction, transient transmembrane channels open. | 1 | |
Mutagenesisi | 286 | F → Y in prlA4-1; altered signal sequence interaction. | 1 | |
Mutagenesisi | 287 | P → L in secY161; altered signal sequence interaction. | 1 | |
Mutagenesisi | 290 | I → T in secY121; altered signal sequence interaction. | 1 | |
Mutagenesisi | 357 | R → H in secY39; cold-sensitive. | 1 | |
Mutagenesisi | 363 | A → S in secY40; cold-sensitive. | 1 | |
Mutagenesisi | 408 | I → N in prlA4-2; altered signal sequence interaction. 1 Publication | 1 | |
Mutagenesisi | 424 – 443 | Missing : No longer complements secY24, a temperature-sensitive secY mutation. 1 PublicationAdd BLAST | 20 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000131721 | 1 – 443 | Protein translocase subunit SecYAdd BLAST | 443 |
Post-translational modificationi
Proteomic databases
jPOSTi | P0AGA2 |
PaxDbi | P0AGA2 |
PRIDEi | P0AGA2 |
Interactioni
Subunit structurei
Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins.
Interacts with the ribosome.
Interacts with SecDF-YajC and YidC; YidC interacts with nascent inner membrane proteins after SecY. The SecDF-YidC-YajC translocase forms a supercomplex with SecYEG, called the holo-translocon (HTL) (PubMed:27435098). The stoichiometry of the super complex may be SecYEG:YidC:SecDF 4:3:1, YajC is in the reconstituted complex (with SecDF) but as no antibody is available it could not be quantified (PubMed:27435098). SecY probably contacts the 23S rRNA and possibly also ribosomal protein L23 during ribosome docking. A single SecY molecule forms the translocating pore, although interaction with SecA may require oligomers.
3 PublicationsBinary interactionsi
P0AGA2
With | #Exp. | IntAct |
---|---|---|
ompA [P0A910] | 2 | EBI-761422,EBI-371347 |
secA [P10408] | 6 | EBI-761422,EBI-543213 |
secE [P0AG96] | 5 | EBI-761422,EBI-6404267 |
secG [P0AG99] | 3 | EBI-761422,EBI-6404248 |
Protein-protein interaction databases
BioGRIDi | 4263404, 365 interactors |
ComplexPortali | CPX-1095, Holo-translocon SecYEG-SecDF-YajC-YidC complex CPX-1096, Protein-conducting channel SecYEG complex |
DIPi | DIP-59302N |
IntActi | P0AGA2, 5 interactors |
STRINGi | 511145.b3300 |
Structurei
Secondary structure
3D structure databases
SMRi | P0AGA2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AGA2 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0201, Bacteria |
HOGENOMi | CLU_030313_0_2_6 |
InParanoidi | P0AGA2 |
PhylomeDBi | P0AGA2 |
Family and domain databases
Gene3Di | 1.10.3370.10, 1 hit |
HAMAPi | MF_01465, SecY, 1 hit |
InterProi | View protein in InterPro IPR026593, SecY IPR002208, SecY/SEC61-alpha IPR030659, SecY_CS IPR023201, SecY_dom_sf |
PANTHERi | PTHR10906, PTHR10906, 1 hit |
Pfami | View protein in Pfam PF00344, SecY, 1 hit |
PIRSFi | PIRSF004557, SecY, 1 hit |
SUPFAMi | SSF103491, SSF103491, 1 hit |
TIGRFAMsi | TIGR00967, 3a0501s007, 1 hit |
PROSITEi | View protein in PROSITE PS00755, SECY_1, 1 hit PS00756, SECY_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAKQPGLDFQ SAKGGLGELK RRLLFVIGAL IVFRIGSFIP IPGIDAAVLA
60 70 80 90 100
KLLEQQRGTI IEMFNMFSGG ALSRASIFAL GIMPYISASI IIQLLTVVHP
110 120 130 140 150
TLAEIKKEGE SGRRKISQYT RYGTLVLAIF QSIGIATGLP NMPGMQGLVI
160 170 180 190 200
NPGFAFYFTA VVSLVTGTMF LMWLGEQITE RGIGNGISII IFAGIVAGLP
210 220 230 240 250
PAIAHTIEQA RQGDLHFLVL LLVAVLVFAV TFFVVFVERG QRRIVVNYAK
260 270 280 290 300
RQQGRRVYAA QSTHLPLKVN MAGVIPAIFA SSIILFPATI ASWFGGGTGW
310 320 330 340 350
NWLTTISLYL QPGQPLYVLL YASAIIFFCF FYTALVFNPR ETADNLKKSG
360 370 380 390 400
AFVPGIRPGE QTAKYIDKVM TRLTLVGALY ITFICLIPEF MRDAMKVPFY
410 420 430 440
FGGTSLLIVV VVIMDFMAQV QTLMMSSQYE SALKKANLKG YGR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01563 Genomic DNA Translation: CAA25725.1 U18997 Genomic DNA Translation: AAA58097.1 U00096 Genomic DNA Translation: AAC76325.1 AP009048 Genomic DNA Translation: BAE77991.1 |
PIRi | A04473, QQECSY |
RefSeqi | NP_417759.1, NC_000913.3 WP_001118861.1, NZ_STEB01000038.1 |
Genome annotation databases
EnsemblBacteriai | AAC76325; AAC76325; b3300 BAE77991; BAE77991; BAE77991 |
GeneIDi | 67415341 947799 |
KEGGi | ecj:JW3262 eco:b3300 |
PATRICi | fig|1411691.4.peg.3431 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X01563 Genomic DNA Translation: CAA25725.1 U18997 Genomic DNA Translation: AAA58097.1 U00096 Genomic DNA Translation: AAC76325.1 AP009048 Genomic DNA Translation: BAE77991.1 |
PIRi | A04473, QQECSY |
RefSeqi | NP_417759.1, NC_000913.3 WP_001118861.1, NZ_STEB01000038.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AKH | electron microscopy | 14.90 | B/Y | 1-436 | [»] | |
2AKI | electron microscopy | 14.90 | B/Y | 1-436 | [»] | |
3J45 | electron microscopy | 9.50 | y | 6-440 | [»] | |
3J46 | electron microscopy | 10.10 | y | 6-440 | [»] | |
4V6M | electron microscopy | - | A | 8-442 | [»] | |
5ABB | electron microscopy | 8.00 | A | 1-443 | [»] | |
5GAE | electron microscopy | 3.33 | g | 1-443 | [»] | |
5MG3 | electron microscopy | 14.00 | Y | 1-443 | [»] | |
5NCO | electron microscopy | 4.80 | g | 14-429 | [»] | |
6R7L | electron microscopy | 6.00 | Y | 1-443 | [»] | |
SMRi | P0AGA2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263404, 365 interactors |
ComplexPortali | CPX-1095, Holo-translocon SecYEG-SecDF-YajC-YidC complex CPX-1096, Protein-conducting channel SecYEG complex |
DIPi | DIP-59302N |
IntActi | P0AGA2, 5 interactors |
STRINGi | 511145.b3300 |
Protein family/group databases
TCDBi | 3.A.5.1.1, the general secretory pathway (sec) family |
Proteomic databases
jPOSTi | P0AGA2 |
PaxDbi | P0AGA2 |
PRIDEi | P0AGA2 |
Genome annotation databases
EnsemblBacteriai | AAC76325; AAC76325; b3300 BAE77991; BAE77991; BAE77991 |
GeneIDi | 67415341 947799 |
KEGGi | ecj:JW3262 eco:b3300 |
PATRICi | fig|1411691.4.peg.3431 |
Organism-specific databases
EchoBASEi | EB0759 |
Phylogenomic databases
eggNOGi | COG0201, Bacteria |
HOGENOMi | CLU_030313_0_2_6 |
InParanoidi | P0AGA2 |
PhylomeDBi | P0AGA2 |
Enzyme and pathway databases
BioCyci | EcoCyc:SECY |
Miscellaneous databases
EvolutionaryTracei | P0AGA2 |
PROi | PR:P0AGA2 |
Family and domain databases
Gene3Di | 1.10.3370.10, 1 hit |
HAMAPi | MF_01465, SecY, 1 hit |
InterProi | View protein in InterPro IPR026593, SecY IPR002208, SecY/SEC61-alpha IPR030659, SecY_CS IPR023201, SecY_dom_sf |
PANTHERi | PTHR10906, PTHR10906, 1 hit |
Pfami | View protein in Pfam PF00344, SecY, 1 hit |
PIRSFi | PIRSF004557, SecY, 1 hit |
SUPFAMi | SSF103491, SSF103491, 1 hit |
TIGRFAMsi | TIGR00967, 3a0501s007, 1 hit |
PROSITEi | View protein in PROSITE PS00755, SECY_1, 1 hit PS00756, SECY_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SECY_ECOLI | |
Accessioni | P0AGA2Primary (citable) accession number: P0AGA2 Secondary accession number(s): P03844, Q2M6W5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families