UniProtKB - P0AG96 (SECE_ECOLI)
Protein translocase subunit SecE
secE
Functioni
Essential subunit of the protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY.
1 PublicationGO - Molecular functioni
- protein transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- intracellular protein transmembrane transport Source: EcoCyc
- intracellular protein transport Source: EcoliWiki
- protein insertion into membrane from inner side Source: EcoCyc
- protein secretion Source: UniProtKB-UniRule
- protein transport by the Sec complex Source: ComplexPortal
- SRP-dependent cotranslational protein targeting to membrane, translocation Source: EcoCyc
Keywordsi
Biological process | Protein transport, Translocation, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:SECE |
Protein family/group databases
TCDBi | 3.A.5.1.1, the general secretory pathway (sec) family |
Names & Taxonomyi
Protein namesi | Recommended name: Protein translocase subunit SecEUniRule annotation |
Gene namesi | Name:secEUniRule annotation Synonyms:prlG Ordered Locus Names:b3981, JW3944 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- cell envelope Sec protein transport complex Source: EcoCyc
- integral component of membrane Source: EcoliWiki
- membrane Source: ComplexPortal
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | Cytoplasmic1 PublicationAdd BLAST | 19 | |
Transmembranei | 20 – 32 | HelicalCuratedAdd BLAST | 13 | |
Topological domaini | 33 – 48 | Periplasmic1 PublicationAdd BLAST | 16 | |
Transmembranei | 49 – 60 | HelicalCuratedAdd BLAST | 12 | |
Topological domaini | 61 – 97 | Cytoplasmic1 PublicationAdd BLAST | 37 | |
Transmembranei | 98 – 115 | HelicalCuratedAdd BLAST | 18 | |
Topological domaini | 116 – 127 | Periplasmic1 PublicationAdd BLAST | 12 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 120 | S → C: Massive ion flux when cross-linked to SecY C-67 mutation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000104163 | 1 – 127 | Protein translocase subunit SecEAdd BLAST | 127 |
Post-translational modificationi
Proteomic databases
jPOSTi | P0AG96 |
PaxDbi | P0AG96 |
PRIDEi | P0AG96 |
Interactioni
Subunit structurei
Part of the essential Sec protein translocation complex which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC/OxaA. Heterotrimer consisting of SecY, SecE and SecG subunits. The SecDF-YidC-YajC translocase forms a supercomplex with SecYEG, called the holo-translocon (HTL) (PubMed:27435098). The stoichiometry of the super complex may be SecYEG:YidC:SecDF 4:3:1, YajC is in the reconstituted complex (with SecDF) but as no antibody is available it could not be quantified (PubMed:27435098). The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. The channel interacts with SecA via subunit SecY. SecE might contact ribosomal protein L23 and/or L29 when the translocation complex is docked with the ribosome.
3 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 4259511, 262 interactors 852780, 1 interactor |
ComplexPortali | CPX-1095, Holo-translocon SecYEG-SecDF-YajC-YidC complex CPX-1096, Protein-conducting channel SecYEG complex |
DIPi | DIP-59303N |
IntActi | P0AG96, 4 interactors |
STRINGi | 511145.b3981 |
Structurei
Secondary structure
3D structure databases
SMRi | P0AG96 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AG96 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0690, Bacteria |
HOGENOMi | CLU_113663_0_1_6 |
InParanoidi | P0AG96 |
PhylomeDBi | P0AG96 |
Family and domain databases
Gene3Di | 1.20.5.1030, 1 hit |
HAMAPi | MF_00422, SecE, 1 hit |
InterProi | View protein in InterPro IPR005807, SecE_bac IPR038379, SecE_sf IPR001901, Translocase_SecE/Sec61-g |
PANTHERi | PTHR33910, PTHR33910, 1 hit |
Pfami | View protein in Pfam PF00584, SecE, 1 hit |
PRINTSi | PR01650, SECETRNLCASE |
TIGRFAMsi | TIGR00964, secE_bact, 1 hit |
PROSITEi | View protein in PROSITE PS01067, SECE_SEC61G, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSANTEAQGS GRGLEAMKWV VVVALLLVAI VGNYLYRDIM LPLRALAVVI
60 70 80 90 100
LIAAAGGVAL LTTKGKATVA FAREARTEVR KVIWPTRQET LHTTLIVAAV
110 120
TAVMSLILWG LDGILVRLVS FITGLRF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30610 Genomic DNA Translation: AAA24621.1 U00006 Genomic DNA Translation: AAC43079.1 U00096 Genomic DNA Translation: AAC76955.1 AP009048 Genomic DNA Translation: BAE77339.1 |
PIRi | A35139, VXECSE |
RefSeqi | NP_418408.1, NC_000913.3 WP_001275702.1, NZ_STEB01000045.1 |
Genome annotation databases
EnsemblBacteriai | AAC76955; AAC76955; b3981 BAE77339; BAE77339; BAE77339 |
GeneIDi | 67415318 948486 |
KEGGi | ecj:JW3944 eco:b3981 |
PATRICi | fig|1411691.4.peg.2731 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30610 Genomic DNA Translation: AAA24621.1 U00006 Genomic DNA Translation: AAC43079.1 U00096 Genomic DNA Translation: AAC76955.1 AP009048 Genomic DNA Translation: BAE77339.1 |
PIRi | A35139, VXECSE |
RefSeqi | NP_418408.1, NC_000913.3 WP_001275702.1, NZ_STEB01000045.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AKH | electron microscopy | 14.90 | C/Z | 17-127 | [»] | |
2AKI | electron microscopy | 14.90 | C/Z | 17-127 | [»] | |
3J45 | electron microscopy | 9.50 | E | 74-127 | [»] | |
3J46 | electron microscopy | 10.10 | E | 74-127 | [»] | |
4V6M | electron microscopy | - | B | 12-127 | [»] | |
5GAE | electron microscopy | 3.33 | h | 1-127 | [»] | |
5MG3 | electron microscopy | 14.00 | E | 3-127 | [»] | |
5NCO | electron microscopy | 4.80 | h | 67-122 | [»] | |
6R7L | electron microscopy | 6.00 | E | 60-127 | [»] | |
SMRi | P0AG96 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259511, 262 interactors 852780, 1 interactor |
ComplexPortali | CPX-1095, Holo-translocon SecYEG-SecDF-YajC-YidC complex CPX-1096, Protein-conducting channel SecYEG complex |
DIPi | DIP-59303N |
IntActi | P0AG96, 4 interactors |
STRINGi | 511145.b3981 |
Protein family/group databases
TCDBi | 3.A.5.1.1, the general secretory pathway (sec) family |
Proteomic databases
jPOSTi | P0AG96 |
PaxDbi | P0AG96 |
PRIDEi | P0AG96 |
Genome annotation databases
EnsemblBacteriai | AAC76955; AAC76955; b3981 BAE77339; BAE77339; BAE77339 |
GeneIDi | 67415318 948486 |
KEGGi | ecj:JW3944 eco:b3981 |
PATRICi | fig|1411691.4.peg.2731 |
Organism-specific databases
EchoBASEi | EB0932 |
Phylogenomic databases
eggNOGi | COG0690, Bacteria |
HOGENOMi | CLU_113663_0_1_6 |
InParanoidi | P0AG96 |
PhylomeDBi | P0AG96 |
Enzyme and pathway databases
BioCyci | EcoCyc:SECE |
Miscellaneous databases
EvolutionaryTracei | P0AG96 |
PROi | PR:P0AG96 |
Family and domain databases
Gene3Di | 1.20.5.1030, 1 hit |
HAMAPi | MF_00422, SecE, 1 hit |
InterProi | View protein in InterPro IPR005807, SecE_bac IPR038379, SecE_sf IPR001901, Translocase_SecE/Sec61-g |
PANTHERi | PTHR33910, PTHR33910, 1 hit |
Pfami | View protein in Pfam PF00584, SecE, 1 hit |
PRINTSi | PR01650, SECETRNLCASE |
TIGRFAMsi | TIGR00964, secE_bact, 1 hit |
PROSITEi | View protein in PROSITE PS01067, SECE_SEC61G, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SECE_ECOLI | |
Accessioni | P0AG96Primary (citable) accession number: P0AG96 Secondary accession number(s): P16920, Q2M8R7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families