UniProtKB - P0AG93 (SECF_ECOLI)
Protein
Protein translocase subunit SecF
Gene
secF
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA. The large periplasmic domain is thought to have a base and head domain joined by a hinge; movement of the hinge may be coupled to both proton transport and protein export, with the head domain capturing substrate, and a conformational change preventing backward movement and driving forward movement. Expression of V.alginolyticus SecD and SecF in E.coli confers Na+-dependent protein export, strongly suggesting SecDF functions via cation-coupled protein translocation.1 Publication
GO - Molecular functioni
GO - Biological processi
- intracellular protein transmembrane transport Source: UniProtKB-UniRule
- protein targeting Source: UniProtKB-UniRule
- protein transport Source: GO_Central
- protein transport by the Sec complex Source: UniProtKB-UniRule
Keywordsi
Biological process | Protein transport, Translocation, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:SECF MetaCyc:SECF |
Protein family/group databases
TCDBi | 2.A.6.4.1, the resistance-nodulation-cell division (rnd) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Protein translocase subunit SecFAlternative name(s): Sec translocon accessory complex subunit SecF |
Gene namesi | Name:secF Ordered Locus Names:b0409, JW0399 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 23 – 43 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 44 – 142 | PeriplasmicSequence analysisAdd BLAST | 99 | |
Transmembranei | 143 – 163 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 164 – 170 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 171 – 191 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 192 – 196 | PeriplasmicSequence analysis | 5 | |
Transmembranei | 197 – 217 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 218 – 247 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 248 – 270 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 271 – 280 | PeriplasmicSequence analysis | 10 | |
Transmembranei | 281 – 301 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 302 – 323 | CytoplasmicSequence analysisAdd BLAST | 22 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Disruption of both secD and SecF confers cold-sensitive growth (strain secD1(Cs)).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 213 | D → M: Abolishes protein translocation. 1 Publication | 1 | |
Mutagenesisi | 247 | R → M: Abolishes protein translocation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095976 | 1 – 323 | Protein translocase subunit SecFAdd BLAST | 323 |
Proteomic databases
jPOSTi | P0AG93 |
PaxDbi | P0AG93 |
PRIDEi | P0AG93 |
Interactioni
Subunit structurei
Part of the prokaryotic protein translocation apparatus which is comprised of SecA, SecB, SecD, SecE, SecF, SecG and SecY. The SecDF-YidC-YajC translocase forms a supercomplex with SecYEG, called the holo-translocon (HTL) (PubMed:27435098). The stoichiometry of the super complex may be SecYEG:YidC:SecDF 4:3:1, YajC is in the reconstituted complex (with SecDF) but as no antibody is available it could not be quantified (PubMed:27435098).
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263101, 312 interactors |
ComplexPortali | CPX-1095, Holo-translocon SecYEG-SecDF-YajC-YidC complex |
STRINGi | 511145.b0409 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0341, Bacteria |
HOGENOMi | CLU_050012_1_0_6 |
InParanoidi | P0AG93 |
PhylomeDBi | P0AG93 |
Family and domain databases
HAMAPi | MF_01464_B, SecF_B, 1 hit |
InterProi | View protein in InterPro IPR022813, SecD/SecF_arch_bac IPR022645, SecD/SecF_bac IPR022646, SecD/SecF_CS IPR005665, SecF_bac |
PANTHERi | PTHR30081, PTHR30081, 1 hit |
Pfami | View protein in Pfam PF07549, Sec_GG, 1 hit PF02355, SecD_SecF, 1 hit |
PRINTSi | PR01755, SECFTRNLCASE |
TIGRFAMsi | TIGR00916, 2A0604s01, 1 hit TIGR00966, 3a0501s07, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AG93-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQEYTVEQL NHGRKVYDFM RWDYWAFGIS GLLLIAAIVI MGVRGFNWGL
60 70 80 90 100
DFTGGTVIEI TLEKPAEIDV MRDALQKAGF EEPMLQNFGS SHDIMVRMPP
110 120 130 140 150
AEGETGGQVL GSQVLKVINE STNQNAAVKR IEFVGPSVGA DLAQTGAMAL
160 170 180 190 200
MAALLSILVY VGFRFEWRLA AGVVIALAHD VIITLGILSL FHIEIDLTIV
210 220 230 240 250
ASLMSVIGYS LNDSIVVSDR IRENFRKIRR GTPYEIFNVS LTQTLHRTLI
260 270 280 290 300
TSGTTLMVIL MLYLFGGPVL EGFSLTMLIG VSIGTASSIY VASALALKLG
310 320
MKREHMLQQK VEKEGADQPS ILP
Sequence cautioni
The sequence AAB40165 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56175 Genomic DNA Translation: CAA39635.1 U82664 Genomic DNA Translation: AAB40165.1 Different initiation. U00096 Genomic DNA Translation: AAC73512.1 AP009048 Genomic DNA Translation: BAE76189.1 |
PIRi | JQ0697 |
RefSeqi | NP_414943.1, NC_000913.3 WP_000046637.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73512; AAC73512; b0409 BAE76189; BAE76189; BAE76189 |
GeneIDi | 58460348 949120 |
KEGGi | ecj:JW0399 eco:b0409 |
PATRICi | fig|1411691.4.peg.1868 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56175 Genomic DNA Translation: CAA39635.1 U82664 Genomic DNA Translation: AAB40165.1 Different initiation. U00096 Genomic DNA Translation: AAC73512.1 AP009048 Genomic DNA Translation: BAE76189.1 |
PIRi | JQ0697 |
RefSeqi | NP_414943.1, NC_000913.3 WP_000046637.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MG3 | electron microscopy | 14.00 | F | 1-323 | [»] | |
SMRi | P0AG93 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263101, 312 interactors |
ComplexPortali | CPX-1095, Holo-translocon SecYEG-SecDF-YajC-YidC complex |
STRINGi | 511145.b0409 |
Protein family/group databases
TCDBi | 2.A.6.4.1, the resistance-nodulation-cell division (rnd) superfamily |
Proteomic databases
jPOSTi | P0AG93 |
PaxDbi | P0AG93 |
PRIDEi | P0AG93 |
Genome annotation databases
EnsemblBacteriai | AAC73512; AAC73512; b0409 BAE76189; BAE76189; BAE76189 |
GeneIDi | 58460348 949120 |
KEGGi | ecj:JW0399 eco:b0409 |
PATRICi | fig|1411691.4.peg.1868 |
Organism-specific databases
EchoBASEi | EB0933 |
Phylogenomic databases
eggNOGi | COG0341, Bacteria |
HOGENOMi | CLU_050012_1_0_6 |
InParanoidi | P0AG93 |
PhylomeDBi | P0AG93 |
Enzyme and pathway databases
BioCyci | EcoCyc:SECF MetaCyc:SECF |
Miscellaneous databases
PROi | PR:P0AG93 |
Family and domain databases
HAMAPi | MF_01464_B, SecF_B, 1 hit |
InterProi | View protein in InterPro IPR022813, SecD/SecF_arch_bac IPR022645, SecD/SecF_bac IPR022646, SecD/SecF_CS IPR005665, SecF_bac |
PANTHERi | PTHR30081, PTHR30081, 1 hit |
Pfami | View protein in Pfam PF07549, Sec_GG, 1 hit PF02355, SecD_SecF, 1 hit |
PRINTSi | PR01755, SECFTRNLCASE |
TIGRFAMsi | TIGR00916, 2A0604s01, 1 hit TIGR00966, 3a0501s07, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SECF_ECOLI | |
Accessioni | P0AG93Primary (citable) accession number: P0AG93 Secondary accession number(s): P19674, P77113, Q2MC17 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families