UniProtKB - P0AG82 (PSTS_ECOLI)
Protein
Phosphate-binding protein PstS
Gene
pstS
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex PstSACB involved in phosphate import.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 63 | PhosphateBy similarity | 1 | |
Binding sitei | 81 | PhosphateBy similarity | 1 |
GO - Molecular functioni
- phosphate ion binding Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- phosphate ion transmembrane transport Source: InterPro
- phosphate ion transport Source: EcoCyc
- regulation of phosphatase activity Source: EcoCyc
- response to radiation Source: EcoCyc
Keywordsi
Biological process | Phosphate transport, Stress response, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:PSTS-MONOMER MetaCyc:PSTS-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.7.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphate-binding protein PstSShort name: PBP |
Gene namesi | Name:pstS Synonyms:phoS Ordered Locus Names:b3728, JW3706 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- ATP-binding cassette (ABC) transporter complex Source: InterPro
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Chemistry databases
DrugBanki | DB02831, Dihydrogenphosphate DB02799, N-[2-(1-Maleimidyl)Ethyl]-7-Diethylaminocoumarin-3-Carboxamide |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | 1 PublicationAdd BLAST | 25 | |
ChainiPRO_0000031848 | 26 – 346 | Phosphate-binding protein PstSAdd BLAST | 321 |
Proteomic databases
jPOSTi | P0AG82 |
PaxDbi | P0AG82 |
PRIDEi | P0AG82 |
2D gel databases
SWISS-2DPAGEi | P0AG82 |
Expressioni
Inductioni
By phosphate deprivation. Positively regulated by PhoB and negatively regulated by PhoR.
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).
CuratedProtein-protein interaction databases
BioGRIDi | 4262139, 97 interactors |
ComplexPortali | CPX-4381, Phosphate ABC transporter complex |
DIPi | DIP-48241N |
IntActi | P0AG82, 2 interactors |
STRINGi | 511145.b3728 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AG82 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AG82 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 36 | Phosphate bindingBy similarity | 3 | |
Regioni | 164 – 166 | Phosphate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the PstS family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0226, Bacteria |
HOGENOMi | CLU_034528_1_0_6 |
InParanoidi | P0AG82 |
PhylomeDBi | P0AG82 |
Family and domain databases
InterProi | View protein in InterPro IPR005673, ABC_phos-bd_PstS IPR024370, PBP_domain |
Pfami | View protein in Pfam PF12849, PBP_like_2, 1 hit |
PIRSFi | PIRSF002756, PstS, 1 hit |
TIGRFAMsi | TIGR00975, 3a0107s03, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AG82-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKVMRTTVAT VVAATLSMSA FSVFAEASLT GAGATFPAPV YAKWADTYQK
60 70 80 90 100
ETGNKVNYQG IGSSGGVKQI IANTVDFGAS DAPLSDEKLA QEGLFQFPTV
110 120 130 140 150
IGGVVLAVNI PGLKSGELVL DGKTLGDIYL GKIKKWDDEA IAKLNPGLKL
160 170 180 190 200
PSQNIAVVRR ADGSGTSFVF TSYLAKVNEE WKNNVGTGST VKWPIGLGGK
210 220 230 240 250
GNDGIAAFVQ RLPGAIGYVE YAYAKQNNLA YTKLISADGK PVSPTEENFA
260 270 280 290 300
NAAKGADWSK TFAQDLTNQK GEDAWPITST TFILIHKDQK KPEQGTEVLK
310 320 330 340
FFDWAYKTGA KQANDLDYAS LPDSVVEQVR AAWKTNIKDS SGKPLY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01992 Genomic DNA Translation: AAA24378.1 L10328 Genomic DNA Translation: AAA62079.1 U00096 Genomic DNA Translation: AAC76751.1 AP009048 Genomic DNA Translation: BAE77560.1 |
PIRi | A30277, BYECPR |
RefSeqi | NP_418184.1, NC_000913.3 WP_000867146.1, NZ_STEB01000015.1 |
Genome annotation databases
EnsemblBacteriai | AAC76751; AAC76751; b3728 BAE77560; BAE77560; BAE77560 |
GeneIDi | 58391163 948237 |
KEGGi | ecj:JW3706 eco:b3728 |
PATRICi | fig|1411691.4.peg.2972 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K01992 Genomic DNA Translation: AAA24378.1 L10328 Genomic DNA Translation: AAA62079.1 U00096 Genomic DNA Translation: AAC76751.1 AP009048 Genomic DNA Translation: BAE77560.1 |
PIRi | A30277, BYECPR |
RefSeqi | NP_418184.1, NC_000913.3 WP_000867146.1, NZ_STEB01000015.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A40 | X-ray | 1.70 | A | 26-346 | [»] | |
1A54 | X-ray | 1.60 | A | 26-346 | [»] | |
1A55 | X-ray | 2.40 | A | 26-346 | [»] | |
1IXG | X-ray | 1.05 | A | 26-346 | [»] | |
1IXH | X-ray | 0.98 | A | 26-346 | [»] | |
1IXI | X-ray | 1.89 | A | 26-346 | [»] | |
1OIB | X-ray | 2.40 | A/B | 26-346 | [»] | |
1PBP | X-ray | 1.90 | A | 26-346 | [»] | |
1QUI | X-ray | 1.90 | A | 26-346 | [»] | |
1QUJ | X-ray | 1.90 | A | 26-346 | [»] | |
1QUK | X-ray | 1.70 | A | 26-346 | [»] | |
1QUL | X-ray | 1.70 | A | 26-346 | [»] | |
2ABH | X-ray | 1.70 | A | 26-346 | [»] | |
SMRi | P0AG82 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262139, 97 interactors |
ComplexPortali | CPX-4381, Phosphate ABC transporter complex |
DIPi | DIP-48241N |
IntActi | P0AG82, 2 interactors |
STRINGi | 511145.b3728 |
Chemistry databases
DrugBanki | DB02831, Dihydrogenphosphate DB02799, N-[2-(1-Maleimidyl)Ethyl]-7-Diethylaminocoumarin-3-Carboxamide |
Protein family/group databases
TCDBi | 3.A.1.7.1, the atp-binding cassette (abc) superfamily |
2D gel databases
SWISS-2DPAGEi | P0AG82 |
Proteomic databases
jPOSTi | P0AG82 |
PaxDbi | P0AG82 |
PRIDEi | P0AG82 |
Genome annotation databases
EnsemblBacteriai | AAC76751; AAC76751; b3728 BAE77560; BAE77560; BAE77560 |
GeneIDi | 58391163 948237 |
KEGGi | ecj:JW3706 eco:b3728 |
PATRICi | fig|1411691.4.peg.2972 |
Organism-specific databases
EchoBASEi | EB0727 |
Phylogenomic databases
eggNOGi | COG0226, Bacteria |
HOGENOMi | CLU_034528_1_0_6 |
InParanoidi | P0AG82 |
PhylomeDBi | P0AG82 |
Enzyme and pathway databases
BioCyci | EcoCyc:PSTS-MONOMER MetaCyc:PSTS-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AG82 |
PROi | PR:P0AG82 |
Family and domain databases
InterProi | View protein in InterPro IPR005673, ABC_phos-bd_PstS IPR024370, PBP_domain |
Pfami | View protein in Pfam PF12849, PBP_like_2, 1 hit |
PIRSFi | PIRSF002756, PstS, 1 hit |
TIGRFAMsi | TIGR00975, 3a0107s03, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSTS_ECOLI | |
Accessioni | P0AG82Primary (citable) accession number: P0AG82 Secondary accession number(s): P06128, P76744, Q2M846 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | February 10, 2021 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families