UniProtKB - P0AG74 (RUSA_ECOLI)
Protein
Crossover junction endodeoxyribonuclease RusA
Gene
rusA
Organism
Escherichia coli (strain K12)
Status
Functioni
Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of ruvAB or ruvC.
Miscellaneous
Encoded by the cryptic lambdoid prophage DLP12. Normally not expressed. Complete suppression of ruv mutations by RusA is dependent on the activity of RecG.
Catalytic activityi
- Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). EC:3.1.22.4
Cofactori
Mg2+CuratedNote: Binds 1 Mg2+ ion per subunit.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 70 | MagnesiumCurated | 1 | |
Metal bindingi | 72 | MagnesiumCurated | 1 | |
Metal bindingi | 91 | MagnesiumCurated | 1 |
GO - Molecular functioni
- crossover junction endodeoxyribonuclease activity Source: EcoCyc
- four-way junction DNA binding Source: EcoCyc
- magnesium ion binding Source: InterPro
GO - Biological processi
- DNA recombination Source: UniProtKB-KW
- DNA repair Source: UniProtKB-KW
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | DNA damage, DNA recombination, DNA repair |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6306-MONOMER |
BRENDAi | 3.1.22.4, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Crossover junction endodeoxyribonuclease RusA (EC:3.1.22.4)Alternative name(s): Holliday junction nuclease RusA Holliday junction resolvase |
Gene namesi | Name:rusA Synonyms:rus, ybcP Ordered Locus Names:b0550, JW0538 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 16 | R → Q: No effect on ability to promote DNA repair. 1 Publication | 1 | |
Mutagenesisi | 17 | Y → L: No effect on ability to promote DNA repair. 1 Publication | 1 | |
Mutagenesisi | 19 | R → Q: No effect on ability to promote DNA repair. 1 Publication | 1 | |
Mutagenesisi | 58 | R → Q: No effect on ability to promote DNA repair. 1 Publication | 1 | |
Mutagenesisi | 68 | R → Q: No effect on ability to promote DNA repair. 1 Publication | 1 | |
Mutagenesisi | 69 | R → Q or A: Loss of ability to promote DNA repair. Great loss of Holliday junction resolvase activity. No effect on DNA binding. 1 Publication | 1 | |
Mutagenesisi | 70 | D → N: Reduces junction resolution 80-fold. No effect on DNA binding. 2 Publications | 1 | |
Mutagenesisi | 72 | D → N: Loss of ability to resolve junctions. No effect on DNA binding. 1 Publication | 1 | |
Mutagenesisi | 73 | N → A: Slight reduction in ability to promote DNA repair. Great reduction in Holliday junction resolution activity. 1 Publication | 1 | |
Mutagenesisi | 76 | K → Q: Loss of ability to promote DNA repair. Loss of Holliday junction resolvase activity. No effect on DNA binding. 1 Publication | 1 | |
Mutagenesisi | 76 | K → R: Loss of ability to promote DNA repair. Less than 2% activity of Holliday junction resolvase. No effect on DNA binding. 1 Publication | 1 | |
Mutagenesisi | 80 | D → N: Slight reduction in ability to resolve junctions. No effect on DNA binding. 1 Publication | 1 | |
Mutagenesisi | 87 | F → Y or V: No effect on ability to promote DNA repair. 1 Publication | 1 | |
Mutagenesisi | 90 | D → N: Slight reduction in ability to resolve junctions. No effect on DNA binding. 1 Publication | 1 | |
Mutagenesisi | 91 | D → N: Loss of ability to resolve junctions. No effect on DNA binding. 1 Publication | 1 | |
Mutagenesisi | 101 | K → Q: No effect on ability to promote DNA repair. 1 Publication | 1 | |
Mutagenesisi | 111 | E → Q: No effect on resolvase activity or DNA binding. 1 Publication | 1 | |
Mutagenesisi | 116 | E → Q: No effect on resolvase activity or DNA binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192004 | 1 – 120 | Crossover junction endodeoxyribonuclease RusAAdd BLAST | 120 |
Proteomic databases
PaxDbi | P0AG74 |
PRIDEi | P0AG74 |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 4261529, 59 interactors 849560, 4 interactors |
DIPi | DIP-16988N |
IntActi | P0AG74, 1 interactor |
STRINGi | 511145.b0550 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AG74 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AG74 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 13 – 16 | DNA binding | 4 | |
Regioni | 66 – 73 | DNA binding | 8 |
Sequence similaritiesi
Belongs to the RusA family.Curated
Phylogenomic databases
eggNOGi | COG4570, Bacteria |
HOGENOMi | CLU_139466_0_2_6 |
InParanoidi | P0AG74 |
Family and domain databases
Gene3Di | 3.30.1330.70, 1 hit |
InterProi | View protein in InterPro IPR016281, Endonuclease_RusA IPR008822, Endonuclease_RusA-like IPR036614, RusA-like_sf |
Pfami | View protein in Pfam PF05866, RusA, 1 hit |
PIRSFi | PIRSF001007, RusA, 1 hit |
SUPFAMi | SSF103084, SSF103084, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AG74-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG
60 70 80 90 100
LAMPVKIRIE CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV
110 120
KMPVTKGGRL ELTITEMGNE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92587 Genomic DNA Translation: CAA63321.1 U82598 Genomic DNA Translation: AAB40746.1 U00096 Genomic DNA Translation: AAC73651.1 AP009048 Genomic DNA Translation: BAE76325.1 |
PIRi | S66590 |
RefSeqi | NP_415082.1, NC_000913.3 WP_001099712.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73651; AAC73651; b0550 BAE76325; BAE76325; BAE76325 |
GeneIDi | 49585103 945174 |
KEGGi | ecj:JW0538 eco:b0550 |
PATRICi | fig|1411691.4.peg.1727 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X92587 Genomic DNA Translation: CAA63321.1 U82598 Genomic DNA Translation: AAB40746.1 U00096 Genomic DNA Translation: AAC73651.1 AP009048 Genomic DNA Translation: BAE76325.1 |
PIRi | S66590 |
RefSeqi | NP_415082.1, NC_000913.3 WP_001099712.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Q8R | X-ray | 1.90 | A/B | 1-120 | [»] | |
2H8C | X-ray | 3.10 | A/B/C/D | 1-120 | [»] | |
2H8E | X-ray | 1.20 | A | 1-120 | [»] | |
SMRi | P0AG74 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261529, 59 interactors 849560, 4 interactors |
DIPi | DIP-16988N |
IntActi | P0AG74, 1 interactor |
STRINGi | 511145.b0550 |
Proteomic databases
PaxDbi | P0AG74 |
PRIDEi | P0AG74 |
Genome annotation databases
EnsemblBacteriai | AAC73651; AAC73651; b0550 BAE76325; BAE76325; BAE76325 |
GeneIDi | 49585103 945174 |
KEGGi | ecj:JW0538 eco:b0550 |
PATRICi | fig|1411691.4.peg.1727 |
Organism-specific databases
EchoBASEi | EB4170 |
Phylogenomic databases
eggNOGi | COG4570, Bacteria |
HOGENOMi | CLU_139466_0_2_6 |
InParanoidi | P0AG74 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6306-MONOMER |
BRENDAi | 3.1.22.4, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0AG74 |
PROi | PR:P0AG74 |
Family and domain databases
Gene3Di | 3.30.1330.70, 1 hit |
InterProi | View protein in InterPro IPR016281, Endonuclease_RusA IPR008822, Endonuclease_RusA-like IPR036614, RusA-like_sf |
Pfami | View protein in Pfam PF05866, RusA, 1 hit |
PIRSFi | PIRSF001007, RusA, 1 hit |
SUPFAMi | SSF103084, SSF103084, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RUSA_ECOLI | |
Accessioni | P0AG74Primary (citable) accession number: P0AG74 Secondary accession number(s): P40116, Q2MBN1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families