UniProtKB - P0AG20 (RELA_ECOLI)
Protein
GTP pyrophosphokinase
Gene
relA
Organism
Escherichia coli (strain K12)
Status
Functioni
In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response which coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp. The second messengers ppGpp and c-di-GMP together control biofilm formation in response to translational stress; ppGpp represses biofilm formation while c-di-GMP induces it. ppGpp activates transcription of CsrA-antagonistic small RNAs CsrB and CsrC, which downregulate CsrA's action on translation during the stringent response (PubMed:21488981).4 Publications
Catalytic activityi
- EC:2.7.6.5
: ppGpp biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Bifunctional (p)ppGpp synthase/hydrolase SpoT (spoT), GTP pyrophosphokinase (relA)
- Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA), Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- GTP binding Source: UniProtKB-KW
- GTP diphosphokinase activity Source: EcoCyc
- guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Source: GO_Central
- kinase activity Source: UniProtKB-KW
GO - Biological processi
- guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
- guanosine tetraphosphate metabolic process Source: EcoCyc
- nucleobase-containing small molecule interconversion Source: EcoCyc
- response to starvation Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:RELA-MONOMER MetaCyc:RELA-MONOMER |
UniPathwayi | UPA00908;UER00884 |
Names & Taxonomyi
Protein namesi | Recommended name: GTP pyrophosphokinase (EC:2.7.6.5)Alternative name(s): (p)ppGpp synthase ATP:GTP 3'-pyrophosphotransferase ppGpp synthase I |
Gene namesi | Name:relA Ordered Locus Names:b2784, JW2755 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
A slight increase in biofilm formation in a csrA-disrupted background; when combined with a spoT disruption (a ppGpp0 mutant) there is a very large increase in biofilm formation (PubMed:19460094). The ppGpp0 mutant makes decreased levels of CsrA and its inhibitory small RNAs (sRNA) CsrB and CsrC (PubMed:21488981). Deletion of relA alone decreases persister cell formation in a hipA7 mutant; the double relA/spoT deletion obviates persister cell formation (PubMed:14622409, PubMed:26051177).4 Publications
Chemistry databases
ChEMBLi | CHEMBL1163114 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166546 | 1 – 744 | GTP pyrophosphokinaseAdd BLAST | 744 |
Proteomic databases
jPOSTi | P0AG20 |
PaxDbi | P0AG20 |
PRIDEi | P0AG20 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259226, 81 interactors |
DIPi | DIP-10658N |
IntActi | P0AG20, 15 interactors |
STRINGi | 511145.b2784 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 55 – 160 | HDPROSITE-ProRule annotationAdd BLAST | 106 | |
Domaini | 404 – 465 | TGSPROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 668 – 743 | ACTPROSITE-ProRule annotationAdd BLAST | 76 |
Sequence similaritiesi
Belongs to the RelA/SpoT family.Curated
Phylogenomic databases
eggNOGi | COG0317, Bacteria |
HOGENOMi | CLU_012300_3_0_6 |
InParanoidi | P0AG20 |
PhylomeDBi | P0AG20 |
Family and domain databases
CDDi | cd05399, NT_Rel-Spo_like, 1 hit cd01668, TGS_RSH, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR002912, ACT_dom IPR012675, Beta-grasp_dom_sf IPR003607, HD/PDEase_dom IPR043519, NT_sf IPR004811, RelA/Spo_fam IPR007685, RelA_SpoT IPR004095, TGS IPR012676, TGS-like IPR033655, TGS_RelA |
Pfami | View protein in Pfam PF13291, ACT_4, 1 hit PF04607, RelA_SpoT, 1 hit PF02824, TGS, 1 hit |
SMARTi | View protein in SMART SM00954, RelA_SpoT, 1 hit |
SUPFAMi | SSF81271, SSF81271, 1 hit SSF81301, SSF81301, 1 hit |
TIGRFAMsi | TIGR00691, spoT_relA, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS51831, HD, 1 hit PS51880, TGS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AG20-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVAVRSAHIN KAGEFDPEKW IASLGITSQK SCECLAETWA YCLQQTQGHP
60 70 80 90 100
DASLLLWRGV EMVEILSTLS MDIDTLRAAL LFPLADANVV SEDVLRESVG
110 120 130 140 150
KSVVNLIHGV RDMAAIRQLK ATHTDSVSSE QVDNVRRMLL AMVDDFRCVV
160 170 180 190 200
IKLAERIAHL REVKDAPEDE RVLAAKECTN IYAPLANRLG IGQLKWELED
210 220 230 240 250
YCFRYLHPTE YKRIAKLLHE RRLDREHYIE EFVGHLRAEM KAEGVKAEVY
260 270 280 290 300
GRPKHIYSIW RKMQKKNLAF DELFDVRAVR IVAERLQDCY AALGIVHTHY
310 320 330 340 350
RHLPDEFDDY VANPKPNGYQ SIHTVVLGPG GKTVEIQIRT KQMHEDAELG
360 370 380 390 400
VAAHWKYKEG AAAGGARSGH EDRIAWLRKL IAWQEEMADS GEMLDEVRSQ
410 420 430 440 450
VFDDRVYVFT PKGDVVDLPA GSTPLDFAYH IHSDVGHRCI GAKIGGRIVP
460 470 480 490 500
FTYQLQMGDQ IEIITQKQPN PSRDWLNPNL GYVTTSRGRS KIHAWFRKQD
510 520 530 540 550
RDKNILAGRQ ILDDELEHLG ISLKEAEKHL LPRYNFNDVD ELLAAIGGGD
560 570 580 590 600
IRLNQMVNFL QSQFNKPSAE EQDAAALKQL QQKSYTPQNR SKDNGRVVVE
610 620 630 640 650
GVGNLMHHIA RCCQPIPGDE IVGFITQGRG ISVHRADCEQ LAELRSHAPE
660 670 680 690 700
RIVDAVWGES YSAGYSLVVR VVANDRSGLL RDITTILANE KVNVLGVASR
710 720 730 740
SDTKQQLATI DMTIEIYNLQ VLGRVLGKLN QVPDVIDARR LHGS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 307 | F → K in AAA03237 (PubMed:2844820).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04039 Unassigned DNA Translation: AAA03237.1 U29580 Genomic DNA Translation: AAA69294.1 U00096 Genomic DNA Translation: AAC75826.1 AP009048 Genomic DNA Translation: BAE76858.1 |
PIRi | D65060, KIECG |
RefSeqi | NP_417264.1, NC_000913.3 WP_000226815.1, NZ_STEB01000030.1 |
Genome annotation databases
EnsemblBacteriai | AAC75826; AAC75826; b2784 BAE76858; BAE76858; BAE76858 |
GeneIDi | 58459983 947244 |
KEGGi | ecj:JW2755 eco:b2784 |
PATRICi | fig|1411691.4.peg.3951 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04039 Unassigned DNA Translation: AAA03237.1 U29580 Genomic DNA Translation: AAA69294.1 U00096 Genomic DNA Translation: AAC75826.1 AP009048 Genomic DNA Translation: BAE76858.1 |
PIRi | D65060, KIECG |
RefSeqi | NP_417264.1, NC_000913.3 WP_000226815.1, NZ_STEB01000030.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5IQR | electron microscopy | 3.00 | 8 | 1-744 | [»] | |
5KPS | electron microscopy | 3.90 | A | 2-744 | [»] | |
5KPV | electron microscopy | 4.10 | 33 | 2-744 | [»] | |
5KPW | electron microscopy | 3.90 | 33 | 2-744 | [»] | |
5KPX | electron microscopy | 3.90 | 33 | 2-744 | [»] | |
5L3P | electron microscopy | 3.70 | z | 1-744 | [»] | |
SMRi | P0AG20 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259226, 81 interactors |
DIPi | DIP-10658N |
IntActi | P0AG20, 15 interactors |
STRINGi | 511145.b2784 |
Chemistry databases
ChEMBLi | CHEMBL1163114 |
Proteomic databases
jPOSTi | P0AG20 |
PaxDbi | P0AG20 |
PRIDEi | P0AG20 |
Genome annotation databases
EnsemblBacteriai | AAC75826; AAC75826; b2784 BAE76858; BAE76858; BAE76858 |
GeneIDi | 58459983 947244 |
KEGGi | ecj:JW2755 eco:b2784 |
PATRICi | fig|1411691.4.peg.3951 |
Organism-specific databases
EchoBASEi | EB0828 |
Phylogenomic databases
eggNOGi | COG0317, Bacteria |
HOGENOMi | CLU_012300_3_0_6 |
InParanoidi | P0AG20 |
PhylomeDBi | P0AG20 |
Enzyme and pathway databases
UniPathwayi | UPA00908;UER00884 |
BioCyci | EcoCyc:RELA-MONOMER MetaCyc:RELA-MONOMER |
Miscellaneous databases
PROi | PR:P0AG20 |
Family and domain databases
CDDi | cd05399, NT_Rel-Spo_like, 1 hit cd01668, TGS_RSH, 1 hit |
Gene3Di | 3.10.20.30, 1 hit 3.30.460.10, 1 hit |
InterProi | View protein in InterPro IPR002912, ACT_dom IPR012675, Beta-grasp_dom_sf IPR003607, HD/PDEase_dom IPR043519, NT_sf IPR004811, RelA/Spo_fam IPR007685, RelA_SpoT IPR004095, TGS IPR012676, TGS-like IPR033655, TGS_RelA |
Pfami | View protein in Pfam PF13291, ACT_4, 1 hit PF04607, RelA_SpoT, 1 hit PF02824, TGS, 1 hit |
SMARTi | View protein in SMART SM00954, RelA_SpoT, 1 hit |
SUPFAMi | SSF81271, SSF81271, 1 hit SSF81301, SSF81301, 1 hit |
TIGRFAMsi | TIGR00691, spoT_relA, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS51831, HD, 1 hit PS51880, TGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RELA_ECOLI | |
Accessioni | P0AG20Primary (citable) accession number: P0AG20 Secondary accession number(s): P11585, Q2MA48 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families