UniProtKB - P0AG14 (SOHB_ECOLI)
Protein
Probable protease SohB
Gene
sohB
Organism
Escherichia coli (strain K12)
Status
Functioni
Multicopy suppressor of the HtrA (DegP) null phenotype. It is possibly a protease, not essential for bacterial viability.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 178 | NucleophileBy similarity | 1 | |
Active sitei | 230 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: InterPro
GO - Biological processi
- proteolysis Source: EcoCyc
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10956-MONOMER |
Protein family/group databases
MEROPSi | S49.002 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:sohB Ordered Locus Names:b1272, JW1264 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | PeriplasmicSequence analysis | 8 | |
Transmembranei | 9 – 29 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 30 – 349 | CytoplasmicSequence analysisAdd BLAST | 320 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171448 | 1 – 349 | Probable protease SohBAdd BLAST | 349 |
Proteomic databases
jPOSTi | P0AG14 |
PaxDbi | P0AG14 |
PRIDEi | P0AG14 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259577, 67 interactors |
DIPi | DIP-35930N |
IntActi | P0AG14, 3 interactors |
STRINGi | 511145.b1272 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S49 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0616, Bacteria |
HOGENOMi | CLU_070316_0_0_6 |
InParanoidi | P0AG14 |
PhylomeDBi | P0AG14 |
Family and domain databases
CDDi | cd07023, S49_Sppa_N_C, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR002142, Peptidase_S49 IPR013703, Peptidase_S49_N_proteobac |
Pfami | View protein in Pfam PF01343, Peptidase_S49, 1 hit PF08496, Peptidase_S49_N, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AG14-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELLSEYGLF LAKIVTVVLA IAAIAAIIVN VAQRNKRQRG ELRVNNLSEQ
60 70 80 90 100
YKEMKEELAA ALMDSHQQKQ WHKAQKKKHK QEAKAAKAKA KLGEVATDSK
110 120 130 140 150
PRVWVLDFKG SMDAHEVNSL REEITAVLAA FKPQDQVVLR LESPGGMVHG
160 170 180 190 200
YGLAASQLQR LRDKNIPLTV TVDKVAASGG YMMACVADKI VSAPFAIVGS
210 220 230 240 250
IGVVAQMPNF NRFLKSKDID IELHTAGQYK RTLTLLGENT EEGREKFREE
260 270 280 290 300
LNETHQLFKD FVKRMRPSLD IEQVATGEHW YGQQAVEKGL VDEINTSDEV
310 320 330 340
ILSLMEGREV VNVRYMQRKR LIDRFTGSAA ESADRLLLRW WQRGQKPLM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 85 | A → R in AAA24639 (PubMed:1885549).Curated | 1 | |
Sequence conflicti | 90 | A → R in AAA24639 (PubMed:1885549).Curated | 1 | |
Sequence conflicti | 133 – 138 | PQDQVV → HQPQVA (PubMed:3029379).Curated | 6 | |
Sequence conflicti | 143 – 154 | SPGGM…GYGLA → ALVAWCMLRAG (PubMed:3029379).CuratedAdd BLAST | 12 | |
Sequence conflicti | 164 | K → R (PubMed:3029379).Curated | 1 | |
Sequence conflicti | 245 – 258 | EKFRE…THQLF → GKIPRRVERNASVV (PubMed:3029379).CuratedAdd BLAST | 14 | |
Sequence conflicti | 296 | T → A in AAA24639 (PubMed:1885549).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73320 Genomic DNA Translation: AAA24639.1 U00096 Genomic DNA Translation: AAC74354.1 AP009048 Genomic DNA Translation: BAA14809.1 |
PIRi | C64875 |
RefSeqi | NP_415788.1, NC_000913.3 WP_000422045.1, NZ_STEB01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAC74354; AAC74354; b1272 BAA14809; BAA14809; BAA14809 |
GeneIDi | 48106219 945858 |
KEGGi | ecj:JW1264 eco:b1272 |
PATRICi | fig|1411691.4.peg.1012 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73320 Genomic DNA Translation: AAA24639.1 U00096 Genomic DNA Translation: AAC74354.1 AP009048 Genomic DNA Translation: BAA14809.1 |
PIRi | C64875 |
RefSeqi | NP_415788.1, NC_000913.3 WP_000422045.1, NZ_STEB01000005.1 |
3D structure databases
SMRi | P0AG14 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259577, 67 interactors |
DIPi | DIP-35930N |
IntActi | P0AG14, 3 interactors |
STRINGi | 511145.b1272 |
Protein family/group databases
MEROPSi | S49.002 |
Proteomic databases
jPOSTi | P0AG14 |
PaxDbi | P0AG14 |
PRIDEi | P0AG14 |
Genome annotation databases
EnsemblBacteriai | AAC74354; AAC74354; b1272 BAA14809; BAA14809; BAA14809 |
GeneIDi | 48106219 945858 |
KEGGi | ecj:JW1264 eco:b1272 |
PATRICi | fig|1411691.4.peg.1012 |
Organism-specific databases
EchoBASEi | EB0949 |
Phylogenomic databases
eggNOGi | COG0616, Bacteria |
HOGENOMi | CLU_070316_0_0_6 |
InParanoidi | P0AG14 |
PhylomeDBi | P0AG14 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10956-MONOMER |
Miscellaneous databases
PROi | PR:P0AG14 |
Family and domain databases
CDDi | cd07023, S49_Sppa_N_C, 1 hit |
InterProi | View protein in InterPro IPR029045, ClpP/crotonase-like_dom_sf IPR002142, Peptidase_S49 IPR013703, Peptidase_S49_N_proteobac |
Pfami | View protein in Pfam PF01343, Peptidase_S49, 1 hit PF08496, Peptidase_S49_N, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SOHB_ECOLI | |
Accessioni | P0AG14Primary (citable) accession number: P0AG14 Secondary accession number(s): P24213, P77676 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries