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Protein

Protein UmuD

Gene

umuD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in UV protection and mutation. Poorly processive, error-prone DNA polymerase involved in translesion repair (PubMed:10801133). Essential for induced (or SOS) mutagenesis. Able to replicate DNA across DNA lesions (thymine photodimers and abasic sites, called translesion synthesis) in the presence of activated RecA; efficiency is maximal in the presence of the beta sliding-clamp and clamp-loading complex of DNA polymerase III plus single-stranded binding protein (SSB) (PubMed:10801133). RecA and to a lesser extent the beta clamp-complex may target Pol V to replication complexes stalled at DNA template lesions (PubMed:10801133).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60For autocatalytic cleavage activity1
Active sitei97For autocatalytic cleavage activity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • DNA repair Source: EcoCyc
  • regulation of transcription, DNA-templated Source: InterPro
  • SOS response Source: EcoCyc
  • translesion synthesis Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processDNA damage, DNA repair, SOS mutagenesis, SOS response

Enzyme and pathway databases

BioCyciEcoCyc:EG11057-MONOMER
MetaCyc:EG11057-MONOMER
BRENDAi3.4.21.B30 2026

Protein family/group databases

MEROPSiS24.003

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UmuD (EC:3.4.21.-)
Alternative name(s):
DNA polymerase V
Short name:
Pol V
Cleaved into the following chain:
Gene namesi
Name:umuD
Ordered Locus Names:b1183, JW1172
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11057 umuD

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27P → D in umuD1; non-cleavable. 1 Publication1
Mutagenesisi65G → R in umuD44; non-cleavable. 1 Publication1
Mutagenesisi92G → D in umuD77; non-cleavable. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000419881 – 139Protein UmuDAdd BLAST139
ChainiPRO_000002730525 – 139Protein UmuD'Add BLAST115

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei24 – 25Cleavage; by autolysis2

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiP0AG11
PRIDEiP0AG11

Expressioni

Inductioni

Repressed by LexA, induced by DNA damage (PubMed:10760155). Induced 1.5-fold by hydroxyurea (PubMed:20005847).2 Publications

Interactioni

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261817, 117 interactors
DIPiDIP-29679N
IntActiP0AG11, 15 interactors
STRINGi316385.ECDH10B_1236

Structurei

Secondary structure

1139
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00626
ProteinModelPortaliP0AG11
SMRiP0AG11
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AG11

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S24 family.Curated

Phylogenomic databases

eggNOGiENOG4105KT5 Bacteria
COG1974 LUCA
HOGENOMiHOG000232166
InParanoidiP0AG11
KOiK03503
PhylomeDBiP0AG11

Family and domain databases

CDDicd06529 S24_LexA-like, 1 hit
InterProiView protein in InterPro
IPR039418 LexA-like
IPR036286 LexA/Signal_pep-like_sf
IPR006197 Peptidase_S24_LexA
IPR015927 Peptidase_S24_S26A/B/C
PfamiView protein in Pfam
PF00717 Peptidase_S24, 1 hit
PRINTSiPR00726 LEXASERPTASE
SUPFAMiSSF51306 SSF51306, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AG11-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLFIKPADLR EIVTFPLFSD LVQCGFPSPA ADYVEQRIDL NQLLIQHPSA
60 70 80 90 100
TYFVKASGDS MIDGGISDGD LLIVDSAITA SHGDIVIAAV DGEFTVKKLQ
110 120 130
LRPTVQLIPM NSAYSPITIS SEDTLDVFGV VIHVVKAMR
Length:139
Mass (Da):15,063
Last modified:November 1, 1986 - v1
Checksum:i0681A3FFAC7ED583
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10107 Genomic DNA Translation: AAA24728.1
M13387 Genomic DNA Translation: AAA98073.1
U00096 Genomic DNA Translation: AAC74267.1
AP009048 Genomic DNA Translation: BAA36030.1
PIRiA03551 ZWECD
RefSeqiNP_415701.1, NC_000913.3
WP_000897378.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74267; AAC74267; b1183
BAA36030; BAA36030; BAA36030
GeneIDi945746
KEGGiecj:JW1172
eco:b1183
PATRICifig|1411691.4.peg.1104

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10107 Genomic DNA Translation: AAA24728.1
M13387 Genomic DNA Translation: AAA98073.1
U00096 Genomic DNA Translation: AAC74267.1
AP009048 Genomic DNA Translation: BAA36030.1
PIRiA03551 ZWECD
RefSeqiNP_415701.1, NC_000913.3
WP_000897378.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AY9X-ray3.00A/B32-139[»]
1I4VNMR-A/B26-139[»]
1UMUX-ray2.50A/B25-137[»]
DisProtiDP00626
ProteinModelPortaliP0AG11
SMRiP0AG11
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261817, 117 interactors
DIPiDIP-29679N
IntActiP0AG11, 15 interactors
STRINGi316385.ECDH10B_1236

Protein family/group databases

MEROPSiS24.003

Proteomic databases

PaxDbiP0AG11
PRIDEiP0AG11

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74267; AAC74267; b1183
BAA36030; BAA36030; BAA36030
GeneIDi945746
KEGGiecj:JW1172
eco:b1183
PATRICifig|1411691.4.peg.1104

Organism-specific databases

EchoBASEiEB1050
EcoGeneiEG11057 umuD

Phylogenomic databases

eggNOGiENOG4105KT5 Bacteria
COG1974 LUCA
HOGENOMiHOG000232166
InParanoidiP0AG11
KOiK03503
PhylomeDBiP0AG11

Enzyme and pathway databases

BioCyciEcoCyc:EG11057-MONOMER
MetaCyc:EG11057-MONOMER
BRENDAi3.4.21.B30 2026

Miscellaneous databases

EvolutionaryTraceiP0AG11
PROiPR:P0AG11

Family and domain databases

CDDicd06529 S24_LexA-like, 1 hit
InterProiView protein in InterPro
IPR039418 LexA-like
IPR036286 LexA/Signal_pep-like_sf
IPR006197 Peptidase_S24_LexA
IPR015927 Peptidase_S24_S26A/B/C
PfamiView protein in Pfam
PF00717 Peptidase_S24, 1 hit
PRINTSiPR00726 LEXASERPTASE
SUPFAMiSSF51306 SSF51306, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUMUD_ECOLI
AccessioniPrimary (citable) accession number: P0AG11
Secondary accession number(s): P04153
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1986
Last sequence update: November 1, 1986
Last modified: November 7, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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