UniProtKB - P0AG03 (UBIX_ECOLI)
Protein
Flavin prenyltransferase UbiX
Gene
ubiX
Organism
Escherichia coli (strain K12)
Status
Functioni
Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN (By similarity). Acts in concert with UbiD to perform the decarboxylation of 4-hydroxy-3-octaprenyl-benzoate, a step in the biosynthesis of coenzyme Q (PubMed:16923914, PubMed:17889824).UniRule annotation2 Publications
Catalytic activityi
- EC:2.5.1.129UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | FMNUniRule annotation | 1 | |
Binding sitei | 123 | FMNUniRule annotation | 1 | |
Binding sitei | 153 | DMAPUniRule annotation | 1 | |
Binding sitei | 169 | DMAPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 12 | FMNUniRule annotation | 3 | |
Nucleotide bindingi | 88 – 91 | FMNUniRule annotation | 4 |
GO - Molecular functioni
- carboxy-lyase activity Source: GO_Central
- flavin prenyltransferase activity Source: EcoCyc
GO - Biological processi
- ubiquinone biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Prenyltransferase, Transferase |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
BioCyci | EcoCyc:UBIX-MONOMER MetaCyc:UBIX-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ubiXUniRule annotation Synonyms:dedF Ordered Locus Names:b2311, JW2308 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene produce very low levels of coenzyme Q8 during logarithmic growth, grow slowly on succinate as the sole carbon source, accumulate 4-hydroxy-3-octaprenyl-benzoate, and have reduced UbiG O-methyltransferase activity. In contrast, they synthesize near normal levels of Q8 in the stationary phase.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000134950 | 1 – 189 | Flavin prenyltransferase UbiXAdd BLAST | 189 |
Proteomic databases
PaxDbi | P0AG03 |
PRIDEi | P0AG03 |
Expressioni
Inductioni
During aerobic growth, expression depends on the carbon source, with the highest expression on succinate, a median expression on glycerol, and the lowest on glucose. During anaerobic growth, glucose does not inhibit expression.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260526, 357 interactors |
DIPi | DIP-47873N |
IntActi | P0AG03, 6 interactors |
STRINGi | 511145.b2311 |
Family & Domainsi
Sequence similaritiesi
Belongs to the UbiX/PAD1 family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0163, Bacteria |
HOGENOMi | CLU_074522_0_1_6 |
InParanoidi | P0AG03 |
PhylomeDBi | P0AG03 |
Family and domain databases
Gene3Di | 3.40.50.1950, 1 hit |
HAMAPi | MF_01984, ubiX_pad, 1 hit |
InterProi | View protein in InterPro IPR036551, Flavin_trans-like IPR003382, Flavoprotein IPR004507, UbiX-like |
PANTHERi | PTHR43374, PTHR43374, 2 hits |
Pfami | View protein in Pfam PF02441, Flavoprotein, 1 hit |
SUPFAMi | SSF52507, SSF52507, 1 hit |
TIGRFAMsi | TIGR00421, ubiX_pad, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AG03-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRLIVGISG ASGAIYGVRL LQVLRDVTDI ETHLVMSQAA RQTLSLETDF
60 70 80 90 100
SLREVQALAD VTHDARDIAA SISSGSFQTL GMVILPCSIK TLSGIVHSYT
110 120 130 140 150
DGLLTRAADV VLKERRPLVL CVRETPLHLG HLRLMTQAAE IGAVIMPPVP
160 170 180
AFYHRPQSLD DVINQTVNRV LDQFAITLPE DLFARWQGA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 62 | T → S in AAA23970 (PubMed:3040734).Curated | 1 | |
Sequence conflicti | 139 – 148 | AEIGAVIMPP → GRNRCGDYAS in AAA23970 (PubMed:3040734).Curated | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M68935 Genomic DNA Translation: AAA23970.1 U00096 Genomic DNA Translation: AAC75371.1 AP009048 Genomic DNA Translation: BAA16157.1 |
PIRi | E65003, XMECFD |
RefSeqi | NP_416814.1, NC_000913.3 WP_000825700.1, NZ_STEB01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC75371; AAC75371; b2311 BAA16157; BAA16157; BAA16157 |
GeneIDi | 58350763 949033 |
KEGGi | ecj:JW2308 eco:b2311 |
PATRICi | fig|511145.12.peg.2406 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M68935 Genomic DNA Translation: AAA23970.1 U00096 Genomic DNA Translation: AAC75371.1 AP009048 Genomic DNA Translation: BAA16157.1 |
PIRi | E65003, XMECFD |
RefSeqi | NP_416814.1, NC_000913.3 WP_000825700.1, NZ_STEB01000008.1 |
3D structure databases
SMRi | P0AG03 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260526, 357 interactors |
DIPi | DIP-47873N |
IntActi | P0AG03, 6 interactors |
STRINGi | 511145.b2311 |
Proteomic databases
PaxDbi | P0AG03 |
PRIDEi | P0AG03 |
Genome annotation databases
EnsemblBacteriai | AAC75371; AAC75371; b2311 BAA16157; BAA16157; BAA16157 |
GeneIDi | 58350763 949033 |
KEGGi | ecj:JW2308 eco:b2311 |
PATRICi | fig|511145.12.peg.2406 |
Organism-specific databases
EchoBASEi | EB1037 |
Phylogenomic databases
eggNOGi | COG0163, Bacteria |
HOGENOMi | CLU_074522_0_1_6 |
InParanoidi | P0AG03 |
PhylomeDBi | P0AG03 |
Enzyme and pathway databases
BioCyci | EcoCyc:UBIX-MONOMER MetaCyc:UBIX-MONOMER |
Miscellaneous databases
PROi | PR:P0AG03 |
Family and domain databases
Gene3Di | 3.40.50.1950, 1 hit |
HAMAPi | MF_01984, ubiX_pad, 1 hit |
InterProi | View protein in InterPro IPR036551, Flavin_trans-like IPR003382, Flavoprotein IPR004507, UbiX-like |
PANTHERi | PTHR43374, PTHR43374, 2 hits |
Pfami | View protein in Pfam PF02441, Flavoprotein, 1 hit |
SUPFAMi | SSF52507, SSF52507, 1 hit |
TIGRFAMsi | TIGR00421, ubiX_pad, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBIX_ECOLI | |
Accessioni | P0AG03Primary (citable) accession number: P0AG03 Secondary accession number(s): P09550, P77715 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families