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Protein

Trk system potassium uptake protein TrkH

Gene

trkH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Low-affinity potassium transport system. Interacts with Trk system potassium uptake protein TrkA. Requires TrkE (sapD) for transport activity, 20% more uptake is seen with both SapD and SapF (PubMed:11700350). Transport in the absence of SapD and SapF is dependent on a high membrane potential and a high cytoplasmic ATP concentration, suggesting this protein may be able to interact with other ATP-binding proteins (PubMed:11700350). Can transport potassium and rubidium (PubMed:7896723).By similarity2 Publications

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=6 mM for K+1 Publication
  2. KM=2 mM for Rb+1 Publication
  1. Vmax=800 µmol/min/g enzyme with K+ as substrate1 Publication
  2. Vmax=10 µmol/min/g enzyme with Rb+ as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi111Potassium; via carbonyl oxygenBy similarity1
Metal bindingi112Potassium; via carbonyl oxygenBy similarity1
Metal bindingi220Potassium; via carbonyl oxygenBy similarity1
Metal bindingi221Potassium; via carbonyl oxygenBy similarity1
Metal bindingi318Potassium; via carbonyl oxygenBy similarity1
Metal bindingi319Potassium; via carbonyl oxygenBy similarity1
Metal bindingi435Potassium; via carbonyl oxygenBy similarity1
Metal bindingi436Potassium; via carbonyl oxygenBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIon channel
Biological processIon transport, Potassium transport, Transport
LigandMetal-binding, Potassium

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:TRKH-MONOMER
MetaCyc:TRKH-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
2.A.38.1.1 the k(+) transporter (trk) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Trk system potassium uptake protein TrkH
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:trkH
Ordered Locus Names:b3849, JW5576
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG11021 trkH

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini? – 483CytoplasmicBy similarity
Topological domaini? – 389CytoplasmicBy similarity
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a region that is buried within a membrane, but does not cross it.<p><a href='/help/intramem' target='_top'>More...</a></p>Intramembranei? – 300By similarity
Topological domaini? – 273CytoplasmicBy similarity
Intramembranei? – 97By similarity
Topological domaini1 – 2CytoplasmicBy similarity2
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei3 – 29HelicalBy similarityAdd BLAST27
Topological domaini30 – 35PeriplasmicBy similarity6
Transmembranei36 – 57HelicalBy similarityAdd BLAST22
Topological domaini58 – 65CytoplasmicBy similarity8
Transmembranei66 – 90HelicalBy similarityAdd BLAST25
Topological domaini91 – ?PeriplasmicBy similarity
Intramembranei98 – 109Helical; Pore-formingBy similarityAdd BLAST12
Intramembranei110 – 115By similarity6
Topological domaini116 – 124PeriplasmicBy similarity9
Transmembranei125 – 150HelicalBy similarityAdd BLAST26
Topological domaini151 – 177CytoplasmicBy similarityAdd BLAST27
Transmembranei178 – 202HelicalBy similarityAdd BLAST25
Topological domaini203 – 205PeriplasmicBy similarity3
Intramembranei206By similarity1
Intramembranei207 – 218Helical; Pore-formingBy similarityAdd BLAST12
Intramembranei219 – 224By similarity6
Topological domaini225 – 234PeriplasmicBy similarity10
Intramembranei235 – 250HelicalBy similarityAdd BLAST16
Intramembranei251 – ?By similarity
Transmembranei274 – 294HelicalBy similarityAdd BLAST21
Topological domaini295 – ?PeriplasmicBy similarity
Intramembranei301 – 316Helical; Pore-formingBy similarityAdd BLAST16
Intramembranei317 – 322By similarity6
Topological domaini323 – 330PeriplasmicBy similarity8
Intramembranei331 – 342HelicalBy similarityAdd BLAST12
Intramembranei343 – 355Note=Loop between two helicesBy similarityAdd BLAST13
Intramembranei356 – ?HelicalBy similarity
Transmembranei390 – 417HelicalBy similarityAdd BLAST28
Topological domaini418 – 419PeriplasmicBy similarity2
Intramembranei420 – 421By similarity2
Intramembranei422 – 432Helical; Pore-formingBy similarityAdd BLAST11
Intramembranei433 – 439By similarity7
Topological domaini440 – 451PeriplasmicBy similarityAdd BLAST12
Intramembranei452 – 463HelicalBy similarityAdd BLAST12
Intramembranei464 – ?By similarity

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000704751 – 483Trk system potassium uptake protein TrkHAdd BLAST483

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P0AFZ7

PRoteomics IDEntifications database

More...
PRIDEi
P0AFZ7

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4262619, 7 interactors

Database of interacting proteins

More...
DIPi
DIP-47978N

Protein interaction database and analysis system

More...
IntActi
P0AFZ7, 2 interactors

STRING: functional protein association networks

More...
STRINGi
316385.ECDH10B_4038

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
P0AFZ7

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni110 – 115Selectivity filter part 1By similarity6
Regioni219 – 224Selectivity filter part 2By similarity6
Regioni317 – 322Selectivity filter part 3By similarity6
Regioni434 – 439Selectivity filter part 4By similarity6

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the TrkH potassium transport family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105D37 Bacteria
COG0168 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000225541

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P0AFZ7

KEGG Orthology (KO)

More...
KOi
K03498

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P0AFZ7

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003445 Cat_transpt
IPR004772 TrkH

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02386 TrkH, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF006247 TrkH, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00933 2a38, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AFZ7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHFRAITRIV GLLVILFSGT MIIPGLVALI YRDGAGRAFT QTFFVALAIG
60 70 80 90 100
SMLWWPNRKE KGELKSREGF LIVVLFWTVL GSVGALPFIF SESPNLTITD
110 120 130 140 150
AFFESFSGLT TTGATTLVGL DSLPHAILFY RQMLQWFGGM GIIVLAVAIL
160 170 180 190 200
PILGVGGMQL YRAEMPGPLK DNKMRPRIAE TAKTLWLIYV LLTVACALAL
210 220 230 240 250
WFAGMDAFDA IGHSFATIAI GGFSTHDASI GYFDSPTINT IIAIFLLISG
260 270 280 290 300
CNYGLHFSLL SGRSLKVYWR DPEFRMFIGV QFTLVVICTL VLWFHNVYSS
310 320 330 340 350
ALMTINQAFF QVVSMATTAG FTTDSIARWP LFLPVLLLCS AFIGGCAGST
360 370 380 390 400
GGGLKVIRIL LLFKQGNREL KRLVHPNAVY SIKLGNRALP ERILEAVWGF
410 420 430 440 450
FSAYALVFIV SMLAIIATGV DDFSAFASVV ATLNNLGPGL GVVADNFTSM
460 470 480
NPVAKWILIA NMLFGRLEVF TLLVLFTPTF WRE
Length:483
Mass (Da):52,960
Last modified:December 20, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1AA9CC2F83EB509A
GO

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA67646 differs from that shown. Reason: Frameshift at position 416.Curated
The sequence X54687 differs from that shown. Reason: Frameshift at positions 418 and 420.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti69Missing in AAA67646 (PubMed:1379743).Curated1
Sequence conflicti144V → IV in X54687 (PubMed:2243799).Curated1
Sequence conflicti196C → S in X54687 (PubMed:2243799).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X54687 Genomic DNA No translation available.
M87049 Genomic DNA Translation: AAA67646.1 Frameshift.
U00096 Genomic DNA Translation: AAT48231.1
AP009048 Genomic DNA Translation: BAE77454.1

Protein sequence database of the Protein Information Resource

More...
PIRi
B65190

NCBI Reference Sequences

More...
RefSeqi
WP_000545677.1, NZ_LN832404.1
YP_026273.1, NC_000913.3

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAT48231; AAT48231; b3849
BAE77454; BAE77454; BAE77454

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
948333

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW5576
eco:b3849

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.2861

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54687 Genomic DNA No translation available.
M87049 Genomic DNA Translation: AAA67646.1 Frameshift.
U00096 Genomic DNA Translation: AAT48231.1
AP009048 Genomic DNA Translation: BAE77454.1
PIRiB65190
RefSeqiWP_000545677.1, NZ_LN832404.1
YP_026273.1, NC_000913.3

3D structure databases

ProteinModelPortaliP0AFZ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262619, 7 interactors
DIPiDIP-47978N
IntActiP0AFZ7, 2 interactors
STRINGi316385.ECDH10B_4038

Protein family/group databases

TCDBi2.A.38.1.1 the k(+) transporter (trk) family

Proteomic databases

PaxDbiP0AFZ7
PRIDEiP0AFZ7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48231; AAT48231; b3849
BAE77454; BAE77454; BAE77454
GeneIDi948333
KEGGiecj:JW5576
eco:b3849
PATRICifig|1411691.4.peg.2861

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB1014
EcoGeneiEG11021 trkH

Phylogenomic databases

eggNOGiENOG4105D37 Bacteria
COG0168 LUCA
HOGENOMiHOG000225541
InParanoidiP0AFZ7
KOiK03498
PhylomeDBiP0AFZ7

Enzyme and pathway databases

BioCyciEcoCyc:TRKH-MONOMER
MetaCyc:TRKH-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P0AFZ7

Family and domain databases

InterProiView protein in InterPro
IPR003445 Cat_transpt
IPR004772 TrkH
PfamiView protein in Pfam
PF02386 TrkH, 1 hit
PIRSFiPIRSF006247 TrkH, 1 hit
TIGRFAMsiTIGR00933 2a38, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTRKH_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AFZ7
Secondary accession number(s): P21166, P76769, Q2M8F2
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 7, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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