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Protein

Trk system potassium uptake protein TrkH

Gene

trkH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Low-affinity potassium transport system. Interacts with Trk system potassium uptake protein TrkA. Requires TrkE (sapD) for transport activity, 20% more uptake is seen with both SapD and SapF (PubMed:11700350). Transport in the absence of SapD and SapF is dependent on a high membrane potential and a high cytoplasmic ATP concentration, suggesting this protein may be able to interact with other ATP-binding proteins (PubMed:11700350). Can transport potassium and rubidium (PubMed:7896723).By similarity2 Publications

Kineticsi

  1. KM=6 mM for K+1 Publication
  2. KM=2 mM for Rb+1 Publication
  1. Vmax=800 µmol/min/g enzyme with K+ as substrate1 Publication
  2. Vmax=10 µmol/min/g enzyme with Rb+ as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi111Potassium; via carbonyl oxygenBy similarity1
Metal bindingi112Potassium; via carbonyl oxygenBy similarity1
Metal bindingi220Potassium; via carbonyl oxygenBy similarity1
Metal bindingi221Potassium; via carbonyl oxygenBy similarity1
Metal bindingi318Potassium; via carbonyl oxygenBy similarity1
Metal bindingi319Potassium; via carbonyl oxygenBy similarity1
Metal bindingi435Potassium; via carbonyl oxygenBy similarity1
Metal bindingi436Potassium; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel
Biological processIon transport, Potassium transport, Transport
LigandMetal-binding, Potassium

Enzyme and pathway databases

BioCyciEcoCyc:TRKH-MONOMER
MetaCyc:TRKH-MONOMER

Protein family/group databases

TCDBi2.A.38.1.1 the k(+) transporter (trk) family

Names & Taxonomyi

Protein namesi
Recommended name:
Trk system potassium uptake protein TrkH
Gene namesi
Name:trkH
Ordered Locus Names:b3849, JW5576
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11021 trkH

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini? – 483CytoplasmicBy similarity
Topological domaini? – 389CytoplasmicBy similarity
Intramembranei? – 300By similarity
Topological domaini? – 273CytoplasmicBy similarity
Intramembranei? – 97By similarity
Topological domaini1 – 2CytoplasmicBy similarity2
Transmembranei3 – 29HelicalBy similarityAdd BLAST27
Topological domaini30 – 35PeriplasmicBy similarity6
Transmembranei36 – 57HelicalBy similarityAdd BLAST22
Topological domaini58 – 65CytoplasmicBy similarity8
Transmembranei66 – 90HelicalBy similarityAdd BLAST25
Topological domaini91 – ?PeriplasmicBy similarity
Intramembranei98 – 109Helical; Pore-formingBy similarityAdd BLAST12
Intramembranei110 – 115By similarity6
Topological domaini116 – 124PeriplasmicBy similarity9
Transmembranei125 – 150HelicalBy similarityAdd BLAST26
Topological domaini151 – 177CytoplasmicBy similarityAdd BLAST27
Transmembranei178 – 202HelicalBy similarityAdd BLAST25
Topological domaini203 – 205PeriplasmicBy similarity3
Intramembranei206By similarity1
Intramembranei207 – 218Helical; Pore-formingBy similarityAdd BLAST12
Intramembranei219 – 224By similarity6
Topological domaini225 – 234PeriplasmicBy similarity10
Intramembranei235 – 250HelicalBy similarityAdd BLAST16
Intramembranei251 – ?By similarity
Transmembranei274 – 294HelicalBy similarityAdd BLAST21
Topological domaini295 – ?PeriplasmicBy similarity
Intramembranei301 – 316Helical; Pore-formingBy similarityAdd BLAST16
Intramembranei317 – 322By similarity6
Topological domaini323 – 330PeriplasmicBy similarity8
Intramembranei331 – 342HelicalBy similarityAdd BLAST12
Intramembranei343 – 355Note=Loop between two helicesBy similarityAdd BLAST13
Intramembranei356 – ?HelicalBy similarity
Transmembranei390 – 417HelicalBy similarityAdd BLAST28
Topological domaini418 – 419PeriplasmicBy similarity2
Intramembranei420 – 421By similarity2
Intramembranei422 – 432Helical; Pore-formingBy similarityAdd BLAST11
Intramembranei433 – 439By similarity7
Topological domaini440 – 451PeriplasmicBy similarityAdd BLAST12
Intramembranei452 – 463HelicalBy similarityAdd BLAST12
Intramembranei464 – ?By similarity

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000704751 – 483Trk system potassium uptake protein TrkHAdd BLAST483

Proteomic databases

PaxDbiP0AFZ7
PRIDEiP0AFZ7

Interactioni

Protein-protein interaction databases

BioGridi4262619, 7 interactors
DIPiDIP-47978N
IntActiP0AFZ7, 2 interactors
STRINGi316385.ECDH10B_4038

Structurei

3D structure databases

ProteinModelPortaliP0AFZ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni110 – 115Selectivity filter part 1By similarity6
Regioni219 – 224Selectivity filter part 2By similarity6
Regioni317 – 322Selectivity filter part 3By similarity6
Regioni434 – 439Selectivity filter part 4By similarity6

Sequence similaritiesi

Belongs to the TrkH potassium transport family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D37 Bacteria
COG0168 LUCA
HOGENOMiHOG000225541
InParanoidiP0AFZ7
KOiK03498
OMAiSMSAWTT
PhylomeDBiP0AFZ7

Family and domain databases

InterProiView protein in InterPro
IPR003445 Cat_transpt
IPR004772 TrkH
PfamiView protein in Pfam
PF02386 TrkH, 1 hit
PIRSFiPIRSF006247 TrkH, 1 hit
TIGRFAMsiTIGR00933 2a38, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFRAITRIV GLLVILFSGT MIIPGLVALI YRDGAGRAFT QTFFVALAIG
60 70 80 90 100
SMLWWPNRKE KGELKSREGF LIVVLFWTVL GSVGALPFIF SESPNLTITD
110 120 130 140 150
AFFESFSGLT TTGATTLVGL DSLPHAILFY RQMLQWFGGM GIIVLAVAIL
160 170 180 190 200
PILGVGGMQL YRAEMPGPLK DNKMRPRIAE TAKTLWLIYV LLTVACALAL
210 220 230 240 250
WFAGMDAFDA IGHSFATIAI GGFSTHDASI GYFDSPTINT IIAIFLLISG
260 270 280 290 300
CNYGLHFSLL SGRSLKVYWR DPEFRMFIGV QFTLVVICTL VLWFHNVYSS
310 320 330 340 350
ALMTINQAFF QVVSMATTAG FTTDSIARWP LFLPVLLLCS AFIGGCAGST
360 370 380 390 400
GGGLKVIRIL LLFKQGNREL KRLVHPNAVY SIKLGNRALP ERILEAVWGF
410 420 430 440 450
FSAYALVFIV SMLAIIATGV DDFSAFASVV ATLNNLGPGL GVVADNFTSM
460 470 480
NPVAKWILIA NMLFGRLEVF TLLVLFTPTF WRE
Length:483
Mass (Da):52,960
Last modified:December 20, 2005 - v1
Checksum:i1AA9CC2F83EB509A
GO

Sequence cautioni

The sequence AAA67646 differs from that shown. Reason: Frameshift at position 416.Curated
The sequence X54687 differs from that shown. Reason: Frameshift at positions 418 and 420.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69Missing in AAA67646 (PubMed:1379743).Curated1
Sequence conflicti144V → IV in X54687 (PubMed:2243799).Curated1
Sequence conflicti196C → S in X54687 (PubMed:2243799).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54687 Genomic DNA No translation available.
M87049 Genomic DNA Translation: AAA67646.1 Frameshift.
U00096 Genomic DNA Translation: AAT48231.1
AP009048 Genomic DNA Translation: BAE77454.1
PIRiB65190
RefSeqiWP_000545677.1, NZ_LN832404.1
YP_026273.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48231; AAT48231; b3849
BAE77454; BAE77454; BAE77454
GeneIDi948333
KEGGiecj:JW5576
eco:b3849
PATRICifig|1411691.4.peg.2861

Similar proteinsi

Entry informationi

Entry nameiTRKH_ECOLI
AccessioniPrimary (citable) accession number: P0AFZ7
Secondary accession number(s): P21166, P76769, Q2M8F2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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