UniProtKB - P0AFY8 (SEQA_ECOLI)
Negative modulator of initiation of replication
seqA
Functioni
Negative regulator of replication initiation, which contributes to regulation of DNA replication and ensures that replication initiation occurs exactly once per chromosome per cell cycle. Binds to pairs of hemimethylated GATC sequences in the oriC region, thus preventing assembly of replication proteins and re-initiation at newly replicated origins. Repression is relieved when the region becomes fully methylated. Can also bind to hemimethylated GATC sequences outside of oriC region. Binds, with less affinity, to fully methylated GATC sites and affects timing of replication. May play a role in chromosome organization and gene regulation.
UniRule annotation6 PublicationsGO - Molecular functioni
- DNA replication origin binding Source: EcoCyc
- double-stranded methylated DNA binding Source: EcoCyc
- hemi-methylated DNA-binding Source: EcoCyc
- identical protein binding Source: IntAct
GO - Biological processi
- negative regulation of DNA-dependent DNA replication initiation Source: EcoCyc
- regulation of transcription, DNA-templated Source: InterPro
- response to radiation Source: EcoCyc
Keywordsi
Molecular function | DNA replication inhibitor, DNA-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12197-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Negative modulator of initiation of replicationUniRule annotation |
Gene namesi | Name:seqAUniRule annotation Ordered Locus Names:b0687, JW0674 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Note: Localization is cell cycle-dependent. Localizes at midcell in newborn cells, then migrates in opposite directions toward cell quarter sites and remains tethered there until the cell divides.
Cytosol
- cytosol Source: EcoCyc
Other locations
- protein-containing complex Source: EcoCyc
- SeqA-DNA complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 116 | R → A: Strongly reduced DNA binding. 1 Publication | 1 | |
Mutagenesisi | 118 | R → A: Strongly reduced DNA binding. 1 Publication | 1 | |
Mutagenesisi | 149 | T → A: Strongly reduced DNA binding. 1 Publication | 1 | |
Mutagenesisi | 150 | N → A or D: Strongly reduced DNA binding. 1 Publication | 1 | |
Mutagenesisi | 151 | T → A: Reduced DNA binding. 1 Publication | 1 | |
Mutagenesisi | 152 | N → D: Strongly reduced DNA binding. 1 Publication | 1 | |
Mutagenesisi | 155 | R → A: Strongly reduced DNA binding. 1 Publication | 1 | |
Mutagenesisi | 156 | K → A: Strongly reduced DNA binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097684 | 1 – 181 | Negative modulator of initiation of replicationAdd BLAST | 181 |
Proteomic databases
jPOSTi | P0AFY8 |
PaxDbi | P0AFY8 |
PRIDEi | P0AFY8 |
Interactioni
Subunit structurei
Homodimer. Polymerizes to form helical filaments. Dimerization is sufficient for DNA-binding, but oligomerization is essential for function.
UniRule annotation4 PublicationsBinary interactionsi
P0AFY8
With | #Exp. | IntAct |
---|---|---|
itself | 13 | EBI-552553,EBI-552553 |
uvrB [P0A8F8] | 2 | EBI-552553,EBI-552176 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4261907, 83 interactors 849651, 2 interactors |
ComplexPortali | CPX-1955, SeqA-DNA complex |
DIPi | DIP-48017N |
IntActi | P0AFY8, 3 interactors |
STRINGi | 511145.b0687 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P0AFY8 |
SMRi | P0AFY8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFY8 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 87 – 88 | Interaction with DNA | 2 | |
Regioni | 116 – 120 | Interaction with DNA | 5 | |
Regioni | 150 – 156 | Interaction with DNA | 7 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG3057, Bacteria |
HOGENOMi | CLU_099733_0_0_6 |
OMAi | RTRTYFA |
PhylomeDBi | P0AFY8 |
Family and domain databases
Gene3Di | 1.10.1220.10, 1 hit 1.20.1380.10, 1 hit |
HAMAPi | MF_00908, SeqA, 1 hit |
InterProi | View protein in InterPro IPR013321, Arc_rbn_hlx_hlx IPR010985, Ribbon_hlx_hlx IPR005621, SeqA IPR026577, SeqA_DNA-bd_C IPR036835, SeqA_DNA-bd_C_sf IPR033761, SeqA_N |
Pfami | View protein in Pfam PF03925, SeqA, 1 hit PF17206, SeqA_N, 1 hit |
PIRSFi | PIRSF019401, SeqA, 1 hit |
SUPFAMi | SSF47598, SSF47598, 1 hit SSF82808, SSF82808, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKTIEVDDEL YSYIASHTKH IGESASDILR RMLKFSAASQ PAAPVTKEVR
60 70 80 90 100
VASPAIVEAK PVKTIKDKVR AMRELLLSDE YAEQKRAVNR FMLLLSTLYS
110 120 130 140 150
LDAQAFAEAT ESLHGRTRVY FAADEQTLLK NGNQTKPKHV PGTPYWVITN
160 170 180
TNTGRKCSMI EHIMQSMQFP AELIEKVCGT I
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07651 Unassigned RNA Translation: AAA19855.1 U00096 Genomic DNA Translation: AAC73781.1 AP009048 Genomic DNA Translation: BAA35336.1 |
PIRi | A54296 |
RefSeqi | NP_415213.1, NC_000913.3 WP_000848387.1, NZ_STEB01000044.1 |
Genome annotation databases
EnsemblBacteriai | AAC73781; AAC73781; b0687 BAA35336; BAA35336; BAA35336 |
GeneIDi | 66671042 945272 |
KEGGi | ecj:JW0674 eco:b0687 |
PATRICi | fig|1411691.4.peg.1589 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07651 Unassigned RNA Translation: AAA19855.1 U00096 Genomic DNA Translation: AAC73781.1 AP009048 Genomic DNA Translation: BAA35336.1 |
PIRi | A54296 |
RefSeqi | NP_415213.1, NC_000913.3 WP_000848387.1, NZ_STEB01000044.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IU3 | X-ray | 3.00 | C/F | 71-181 | [»] | |
1J3E | X-ray | 2.50 | A | 71-181 | [»] | |
1LRR | X-ray | 2.65 | A/D | 51-181 | [»] | |
1XRX | X-ray | 2.15 | A/B/C/D | 1-50 | [»] | |
3FMT | X-ray | 2.98 | A/B/E/F | 1-181 | [»] | |
AlphaFoldDBi | P0AFY8 | |||||
SMRi | P0AFY8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261907, 83 interactors 849651, 2 interactors |
ComplexPortali | CPX-1955, SeqA-DNA complex |
DIPi | DIP-48017N |
IntActi | P0AFY8, 3 interactors |
STRINGi | 511145.b0687 |
Proteomic databases
jPOSTi | P0AFY8 |
PaxDbi | P0AFY8 |
PRIDEi | P0AFY8 |
Genome annotation databases
EnsemblBacteriai | AAC73781; AAC73781; b0687 BAA35336; BAA35336; BAA35336 |
GeneIDi | 66671042 945272 |
KEGGi | ecj:JW0674 eco:b0687 |
PATRICi | fig|1411691.4.peg.1589 |
Organism-specific databases
EchoBASEi | EB2114 |
Phylogenomic databases
eggNOGi | COG3057, Bacteria |
HOGENOMi | CLU_099733_0_0_6 |
OMAi | RTRTYFA |
PhylomeDBi | P0AFY8 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12197-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFY8 |
PROi | PR:P0AFY8 |
Family and domain databases
Gene3Di | 1.10.1220.10, 1 hit 1.20.1380.10, 1 hit |
HAMAPi | MF_00908, SeqA, 1 hit |
InterProi | View protein in InterPro IPR013321, Arc_rbn_hlx_hlx IPR010985, Ribbon_hlx_hlx IPR005621, SeqA IPR026577, SeqA_DNA-bd_C IPR036835, SeqA_DNA-bd_C_sf IPR033761, SeqA_N |
Pfami | View protein in Pfam PF03925, SeqA, 1 hit PF17206, SeqA_N, 1 hit |
PIRSFi | PIRSF019401, SeqA, 1 hit |
SUPFAMi | SSF47598, SSF47598, 1 hit SSF82808, SSF82808, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SEQA_ECOLI | |
Accessioni | P0AFY8Primary (citable) accession number: P0AFY8 Secondary accession number(s): P36658 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | May 25, 2022 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families