UniProtKB - P0AFX7 (RSEA_ECOLI)
Anti-sigma-E factor RseA
rseA
Functioni
An anti-sigma factor for extracytoplasmic function (ECF) sigma factor sigma-E (RpoE). ECF sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis (RIP). RIP occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The membrane-spanning regulatory substrate protein is first cut periplasmically (site-1 protease, S1P, DegS), then within the membrane itself (site-2 protease, S2P, RseP), while cytoplasmic proteases finish degrading the anti-sigma factor, liberating sigma-E. Overexpression of RseA blocks sigma-E from acting, results in cell lysis in stationary phase and temperature-sensitivity above 37 degrees Celsius.
3 PublicationsCaution
GO - Molecular functioni
- sigma factor antagonist activity Source: EcoCyc
Keywordsi
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12341-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Anti-sigma-E factor RseAAlternative name(s): Regulator of SigE Sigma-E anti-sigma factor RseA Sigma-E factor negative regulatory protein |
Gene namesi | Name:rseA Synonyms:mclA, yfiJ Ordered Locus Names:b2572, JW2556 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 4 Publications; Single-pass type II membrane protein 4 Publications
Note: Following cleavage by DegS the large fragment of the protein is still in the inner membrane and retains its anti-sigma-E activity.
Plasma Membrane
- plasma membrane Source: CACAO
Other locations
- integral component of membrane Source: UniProtKB-KW
- sigma factor antagonist complex Source: ComplexPortal
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 100 | Cytoplasmic1 PublicationAdd BLAST | 100 | |
Transmembranei | 101 – 118 | Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST | 18 | |
Topological domaini | 119 – 216 | Periplasmic1 PublicationAdd BLAST | 98 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 – 28 | Missing : Loss of anti-sigma factor activity. 1 PublicationAdd BLAST | 28 | |
Mutagenesisi | 11 | D → H: Loss of anti-sigma factor activity. 1 Publication | 1 | |
Mutagenesisi | 19 | L → P: Loss of anti-sigma factor activity. 1 Publication | 1 | |
Mutagenesisi | 33 | W → C: Loss of anti-sigma factor activity. 1 Publication | 1 | |
Mutagenesisi | 79 | Q → A: No binding of N-terminal fragment to SspB. 1 Publication | 1 | |
Mutagenesisi | 85 | W → A: No binding of N-terminal fragment to SspB. 1 Publication | 1 | |
Mutagenesisi | 88 | M → A: Reduced binding of N-terminal fragment to SspB. 1 Publication | 1 | |
Mutagenesisi | 107 – 108 | AA → DD: Significantly less degradation by ClpX-ClpP when present as a 1-108 peptide fragment. 1 Publication | 2 | |
Mutagenesisi | 146 | S → X: No effect on protein cleavage. 1 Publication | 1 | |
Mutagenesisi | 147 | P → X: No effect on protein cleavage in vitro and in vivo. 2 Publications | 1 | |
Mutagenesisi | 148 | V → A, C, I, L, M, N or T: Normal cleavage by DegS and RseP in vitro (PubMed:19706448), for A-148 decreased cleavage by DegS in vivo (PubMed:23016873). 2 Publications | 1 | |
Mutagenesisi | 148 | V → D, E, G, F or P: Not cleaved by DegS nor RseP in vitro (PubMed:19706448). 2 Publications | 1 | |
Mutagenesisi | 148 | V → H, K, Q, R, S or Y: Cleaved by DegS but not by RseP in vitro (PubMed:19706448), for R-148 and S-148 decreased cleavage by DegS in vivo (PubMed:23016873). 2 Publications | 1 | |
Mutagenesisi | 162 – 169 | QQQQVQEQ → AAAAVAEA: RseA is degraded by RseP in the absence of DegS. 1 Publication | 8 | |
Mutagenesisi | 172 | R → A: Still binds RseB. No binding to RseB; when associated with A-185. 1 Publication | 1 | |
Mutagenesisi | 172 | R → D: No binding to RseB. 1 Publication | 1 | |
Mutagenesisi | 185 | R → A: Still binds RseB. No binding to RseB; when associated with A-172. 1 Publication | 1 | |
Mutagenesisi | 185 | R → E: No binding to RseB. 1 Publication | 1 | |
Mutagenesisi | 190 – 200 | QLQFEQAQTQQ → ALAFFAAATAA: RseA is degraded by RseP in the absence of DegS. 1 PublicationAdd BLAST | 11 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097480 | 1 – 216 | Anti-sigma-E factor RseAAdd BLAST | 216 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 108 – 109 | Cleavage; by RseP | 2 | |
Sitei | 148 – 149 | Cleavage; by DegS | 2 |
Proteomic databases
jPOSTi | P0AFX7 |
PaxDbi | P0AFX7 |
PRIDEi | P0AFX7 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Interacts 1:1 with ECF RNA polymerase sigma-E (RpoE); this inhibits the interaction of sigma-E with the RNA polymerase catalytic core and leads to a decreased expression of sigma-E-regulated genes.
Interacts with RseB with 1:1 stoichiometry. The liberated N-terminus (residues 1-108) forms a complex with SspB and RpoE; binding to SspB is competitively inhibited by ssrA-tags, although the 2 proteins bind in opposite directions in SspB's peptide-binding groove.
14 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 4261964, 1 interactor 851391, 1 interactor |
ComplexPortali | CPX-2532, rpoe-rsea-rseb sigma-antisigma complex |
DIPi | DIP-39581N |
IntActi | P0AFX7, 6 interactors |
STRINGi | 511145.b2572 |
Structurei
Secondary structure
3D structure databases
SMRi | P0AFX7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFX7 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 97 | Sufficient to repress sigma-EAdd BLAST | 97 | |
Regioni | 146 – 166 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 169 – 186 | Primary binding site for RseBAdd BLAST | 18 | |
Regioni | 190 – 200 | Poly-Gln (Q2)Add BLAST | 11 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 158 – 202 | Sequence analysisAdd BLAST | 45 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 152 – 166 | Polar residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3073, Bacteria |
HOGENOMi | CLU_108851_1_0_6 |
InParanoidi | P0AFX7 |
PhylomeDBi | P0AFX7 |
Family and domain databases
CDDi | cd16328, RseA_N, 1 hit |
DisProti | DP00552 |
Gene3Di | 1.10.10.880, 1 hit |
InterProi | View protein in InterPro IPR005573, Anti-sigma_E_RseA_C IPR005572, Anti-sigma_E_RseA_N IPR036147, Anti-sigma_E_RseA_N_sf IPR026279, RseA |
Pfami | View protein in Pfam PF03873, RseA_C, 1 hit PF03872, RseA_N, 1 hit |
PIRSFi | PIRSF016938, RseA, 1 hit |
SUPFAMi | SSF89069, SSF89069, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MQKEQLSALM DGETLDSELL NELAHNPEMQ KTWESYHLIR DSMRGDTPEV
60 70 80 90 100
LHFDISSRVM AAIEEEPVRQ PATLIPEAQP APHQWQKMPF WQKVRPWAAQ
110 120 130 140 150
LTQMGVAACV SLAVIVGVQH YNGQSETSQQ PETPVFNTLP MMGKASPVSL
160 170 180 190 200
GVPSEATANN GQQQQVQEQR RRINAMLQDY ELQRRLHSEQ LQFEQAQTQQ
210
AAVQVPGIQT LGTQSQ
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10148 Genomic DNA Translation: AAA83999.1 U37089 Genomic DNA Translation: AAC45315.1 D64044 Genomic DNA Translation: BAA10919.1 D13169 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC75625.1 AP009048 Genomic DNA Translation: BAE76748.1 U37455 Genomic DNA Translation: AAC45318.1 |
PIRi | B57255 |
RefSeqi | NP_417067.1, NC_000913.3 WP_001168459.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75625; AAC75625; b2572 BAE76748; BAE76748; BAE76748 |
GeneIDi | 66673539 947053 |
KEGGi | ecj:JW2556 eco:b2572 |
PATRICi | fig|1411691.4.peg.4162 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10148 Genomic DNA Translation: AAA83999.1 U37089 Genomic DNA Translation: AAC45315.1 D64044 Genomic DNA Translation: BAA10919.1 D13169 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC75625.1 AP009048 Genomic DNA Translation: BAE76748.1 U37455 Genomic DNA Translation: AAC45318.1 |
PIRi | B57255 |
RefSeqi | NP_417067.1, NC_000913.3 WP_001168459.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OR7 | X-ray | 2.00 | C/F | 1-90 | [»] | |
1YFN | X-ray | 1.80 | E/F/G/H | 77-108 | [»] | |
3M4W | X-ray | 2.30 | E/F/G/H | 121-216 | [»] | |
SMRi | P0AFX7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261964, 1 interactor 851391, 1 interactor |
ComplexPortali | CPX-2532, rpoe-rsea-rseb sigma-antisigma complex |
DIPi | DIP-39581N |
IntActi | P0AFX7, 6 interactors |
STRINGi | 511145.b2572 |
Proteomic databases
jPOSTi | P0AFX7 |
PaxDbi | P0AFX7 |
PRIDEi | P0AFX7 |
Genome annotation databases
EnsemblBacteriai | AAC75625; AAC75625; b2572 BAE76748; BAE76748; BAE76748 |
GeneIDi | 66673539 947053 |
KEGGi | ecj:JW2556 eco:b2572 |
PATRICi | fig|1411691.4.peg.4162 |
Organism-specific databases
EchoBASEi | EB2245 |
Phylogenomic databases
eggNOGi | COG3073, Bacteria |
HOGENOMi | CLU_108851_1_0_6 |
InParanoidi | P0AFX7 |
PhylomeDBi | P0AFX7 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12341-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFX7 |
PROi | PR:P0AFX7 |
Family and domain databases
CDDi | cd16328, RseA_N, 1 hit |
DisProti | DP00552 |
Gene3Di | 1.10.10.880, 1 hit |
InterProi | View protein in InterPro IPR005573, Anti-sigma_E_RseA_C IPR005572, Anti-sigma_E_RseA_N IPR036147, Anti-sigma_E_RseA_N_sf IPR026279, RseA |
Pfami | View protein in Pfam PF03873, RseA_C, 1 hit PF03872, RseA_N, 1 hit |
PIRSFi | PIRSF016938, RseA, 1 hit |
SUPFAMi | SSF89069, SSF89069, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RSEA_ECOLI | |
Accessioni | P0AFX7Primary (citable) accession number: P0AFX7 Secondary accession number(s): P38106, Q2MAF8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 23, 2022 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families