UniProtKB - P0AFX0 (HPF_ECOLI)
Protein
Ribosome hibernation promoting factor
Gene
hpf
Organism
Escherichia coli (strain K12)
Status
Functioni
During stationary phase, promotes and stabilizes dimerization of 70S ribosomes by the ribosome modulation factor (RMF), leading to the formation of inactive 100S ribosomes (PubMed:18174192). Converts immature 90S particles formed by RMF into 100S ribosomes (PubMed:16324148). Crystallization with T.thermophilus 70S ribosomes shows it binds in the channel between the head and body of the 30S subunit, where mRNA, tRNAs, initiation factors IF1 and IF3 and elongation factor G would bind; however RMF is still able to bind (PubMed:22605777). In this crystal binding of HPF induces movement of the 30S head domain away from the rest of the ribosome, presumably so they would more easily form dimers (PubMed:22605777). May also function as a potential translational inhibitor (PubMed:18174192).5 Publications
GO - Molecular functioni
- ribosomal small subunit binding Source: EcoCyc
- ribosome binding Source: EcoCyc
GO - Biological processi
- negative regulation of translation Source: EcoCyc
- negative regulation of translational elongation Source: GO_Central
- primary metabolic process Source: InterPro
Keywordsi
Biological process | Translation regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11681-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosome hibernation promoting factor1 PublicationShort name: HPF1 Publication Alternative name(s): Hibernation factor HPF1 Publication |
Gene namesi | Name:hpf1 Publication Synonyms:yhbH Ordered Locus Names:b3203, JW3170 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: EcoCyc
- cytosolic small ribosomal subunit Source: GO_Central
Pathology & Biotechi
Disruption phenotypei
Non-essential gene, no formation of inactive 100S ribosomes. Double hpf-yfiA deletion mutants form 90S ribosomes (PubMed:16324148). A quadruple yfiA-hpf-rmf-sra knockout strain is significantly outcompeted by wild-type after 4 days growth (PubMed:17277072). No change in sensitivity to aminoglycoside antiobiotic gentamicin in stationary phase cultures (PubMed:26324267).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097417 | 1 – 95 | Ribosome hibernation promoting factorAdd BLAST | 95 |
Proteomic databases
jPOSTi | P0AFX0 |
PaxDbi | P0AFX0 |
PRIDEi | P0AFX0 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Associates exclusively with 100S ribosomes, which are dimers of 70S ribosomes.
2 PublicationsBinary interactionsi
P0AFX0
With | #Exp. | IntAct |
---|---|---|
dnaN [P0A988] | 2 | EBI-561113,EBI-542385 |
Protein-protein interaction databases
BioGRIDi | 4259283, 29 interactors |
DIPi | DIP-48273N |
IntActi | P0AFX0, 4 interactors |
STRINGi | 511145.b3203 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0AFX0 |
SMRi | P0AFX0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFX0 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1544, Bacteria |
HOGENOMi | CLU_071472_3_1_6 |
InParanoidi | P0AFX0 |
PhylomeDBi | P0AFX0 |
Family and domain databases
CDDi | cd00552, RaiA, 1 hit |
Gene3Di | 3.30.160.100, 1 hit |
InterProi | View protein in InterPro IPR036567, RHF-like IPR003489, RHF/RaiA |
Pfami | View protein in Pfam PF02482, Ribosomal_S30AE, 1 hit |
SUPFAMi | SSF69754, SSF69754, 1 hit |
TIGRFAMsi | TIGR00741, yfiA, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFX0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQLNITGNNV EITEALREFV TAKFAKLEQY FDRINQVYVV LKVEKVTHTS
60 70 80 90
DATLHVNGGE IHASAEGQDM YAAIDGLIDK LARQLTKHKD KLKQH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12938 Genomic DNA Translation: BAA02316.1 Z27094 Genomic DNA Translation: CAA81618.1 U12684 Genomic DNA Translation: AAB60164.1 U18997 Genomic DNA Translation: AAA58005.1 U00096 Genomic DNA Translation: AAC76235.1 AP009048 Genomic DNA Translation: BAE77247.1 |
PIRi | I76719 |
RefSeqi | NP_417670.1, NC_000913.3 WP_001176599.1, NZ_STEB01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC76235; AAC76235; b3203 BAE77247; BAE77247; BAE77247 |
GeneIDi | 49582835 947718 |
KEGGi | ecj:JW3170 eco:b3203 |
PATRICi | fig|1411691.4.peg.3528 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12938 Genomic DNA Translation: BAA02316.1 Z27094 Genomic DNA Translation: CAA81618.1 U12684 Genomic DNA Translation: AAB60164.1 U18997 Genomic DNA Translation: AAA58005.1 U00096 Genomic DNA Translation: AAC76235.1 AP009048 Genomic DNA Translation: BAE77247.1 |
PIRi | I76719 |
RefSeqi | NP_417670.1, NC_000913.3 WP_001176599.1, NZ_STEB01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2RQL | NMR | - | A | 1-95 | [»] | |
4V8H | X-ray | 3.10 | AX/CX | 1-95 | [»] | |
6H4N | electron microscopy | 3.00 | x | 1-95 | [»] | |
6H58 | electron microscopy | 7.90 | x/xx | 1-95 | [»] | |
6Y69 | electron microscopy | 2.86 | x | 1-95 | [»] | |
BMRBi | P0AFX0 | |||||
SMRi | P0AFX0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259283, 29 interactors |
DIPi | DIP-48273N |
IntActi | P0AFX0, 4 interactors |
STRINGi | 511145.b3203 |
Proteomic databases
jPOSTi | P0AFX0 |
PaxDbi | P0AFX0 |
PRIDEi | P0AFX0 |
Genome annotation databases
EnsemblBacteriai | AAC76235; AAC76235; b3203 BAE77247; BAE77247; BAE77247 |
GeneIDi | 49582835 947718 |
KEGGi | ecj:JW3170 eco:b3203 |
PATRICi | fig|1411691.4.peg.3528 |
Organism-specific databases
EchoBASEi | EB1632 |
Phylogenomic databases
eggNOGi | COG1544, Bacteria |
HOGENOMi | CLU_071472_3_1_6 |
InParanoidi | P0AFX0 |
PhylomeDBi | P0AFX0 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11681-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFX0 |
PROi | PR:P0AFX0 |
Family and domain databases
CDDi | cd00552, RaiA, 1 hit |
Gene3Di | 3.30.160.100, 1 hit |
InterProi | View protein in InterPro IPR036567, RHF-like IPR003489, RHF/RaiA |
Pfami | View protein in Pfam PF02482, Ribosomal_S30AE, 1 hit |
SUPFAMi | SSF69754, SSF69754, 1 hit |
TIGRFAMsi | TIGR00741, yfiA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HPF_ECOLI | |
Accessioni | P0AFX0Primary (citable) accession number: P0AFX0 Secondary accession number(s): P31221, Q2M909 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families