UniProtKB - P0AFW4 (RNK_ECOLI)
Protein
Regulator of nucleoside diphosphate kinase
Gene
rnk
Organism
Escherichia coli (strain K12)
Status
Functioni
May act as an anti-Gre factor. Regulates the level of the nucleoside diphosphate kinase Ndk.UniRule annotation2 Publications
Miscellaneous
Can restore alginate synthesis and Ndk activity in the aglQ mutant of P.aeruginosa.1 Publication
GO - Molecular functioni
- DNA binding Source: InterPro
- RNA polymerase binding Source: InterPro
GO - Biological processi
- DNA-templated transcription, elongation Source: GO_Central
- regulation of DNA-templated transcription, elongation Source: InterPro
Enzyme and pathway databases
BioCyci | EcoCyc:G6337-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Regulator of nucleoside diphosphate kinaseUniRule annotation |
Gene namesi | Name:rnkUniRule annotation Ordered Locus Names:b0610, JW0602 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutant shows a significant reduction in the level of Ndk.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097386 | 1 – 136 | Regulator of nucleoside diphosphate kinaseAdd BLAST | 136 |
Proteomic databases
jPOSTi | P0AFW4 |
PaxDbi | P0AFW4 |
PRIDEi | P0AFW4 |
Interactioni
Subunit structurei
Interacts with the RNA polymerase.
UniRule annotation1 PublicationBinary interactionsi
Hide detailsGO - Molecular functioni
- RNA polymerase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 4259900, 11 interactors 851863, 7 interactors |
DIPi | DIP-48048N |
IntActi | P0AFW4, 28 interactors |
STRINGi | 511145.b0610 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFW4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFW4 |
Family & Domainsi
Domaini
Contains a short N-terminal coiled-coil domain and a C-terminal globular domain.1 Publication
Sequence similaritiesi
Belongs to the Rnk family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0782, Bacteria |
HOGENOMi | CLU_120358_1_1_6 |
InParanoidi | P0AFW4 |
PhylomeDBi | P0AFW4 |
Family and domain databases
Gene3Di | 3.10.50.30, 1 hit |
HAMAPi | MF_00954, Rnk, 1 hit |
InterProi | View protein in InterPro IPR036953, GreA/GreB_C_sf IPR028625, Rnk IPR029462, Rnk_N IPR001437, Tscrpt_elong_fac_GreA/B_C IPR023459, Tscrpt_elong_fac_GreA/B_fam |
PANTHERi | PTHR30437, PTHR30437, 1 hit |
Pfami | View protein in Pfam PF01272, GreA_GreB, 1 hit PF14760, Rnk_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFW4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRPTIIIND LDAERIDILL EQPAYAGLPI ADALNAELDR AQMCSPEEMP
60 70 80 90 100
HDVVTMNSRV KFRNLSDGEV RVRTLVYPAK MTDSNTQLSV MAPVGAALLG
110 120 130
LRVGDSIHWE LPGGVATHLE VLELEYQPEA AGDYLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37900 Genomic DNA Translation: AAC36933.1 U82598 Genomic DNA Translation: AAB40810.1 U00096 Genomic DNA Translation: AAC73711.1 AP009048 Genomic DNA Translation: BAA35239.1 |
PIRi | I57917 |
RefSeqi | NP_415143.1, NC_000913.3 WP_000089731.1, NZ_STEB01000031.1 |
Genome annotation databases
EnsemblBacteriai | AAC73711; AAC73711; b0610 BAA35239; BAA35239; BAA35239 |
GeneIDi | 58390771 947546 |
KEGGi | ecj:JW0602 eco:b0610 |
PATRICi | fig|1411691.4.peg.1658 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L37900 Genomic DNA Translation: AAC36933.1 U82598 Genomic DNA Translation: AAB40810.1 U00096 Genomic DNA Translation: AAC73711.1 AP009048 Genomic DNA Translation: BAA35239.1 |
PIRi | I57917 |
RefSeqi | NP_415143.1, NC_000913.3 WP_000089731.1, NZ_STEB01000031.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3BMB | X-ray | 1.91 | A/B | 1-136 | [»] | |
SMRi | P0AFW4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259900, 11 interactors 851863, 7 interactors |
DIPi | DIP-48048N |
IntActi | P0AFW4, 28 interactors |
STRINGi | 511145.b0610 |
Proteomic databases
jPOSTi | P0AFW4 |
PaxDbi | P0AFW4 |
PRIDEi | P0AFW4 |
Genome annotation databases
EnsemblBacteriai | AAC73711; AAC73711; b0610 BAA35239; BAA35239; BAA35239 |
GeneIDi | 58390771 947546 |
KEGGi | ecj:JW0602 eco:b0610 |
PATRICi | fig|1411691.4.peg.1658 |
Organism-specific databases
EchoBASEi | EB2518 |
Phylogenomic databases
eggNOGi | COG0782, Bacteria |
HOGENOMi | CLU_120358_1_1_6 |
InParanoidi | P0AFW4 |
PhylomeDBi | P0AFW4 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6337-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFW4 |
PROi | PR:P0AFW4 |
Family and domain databases
Gene3Di | 3.10.50.30, 1 hit |
HAMAPi | MF_00954, Rnk, 1 hit |
InterProi | View protein in InterPro IPR036953, GreA/GreB_C_sf IPR028625, Rnk IPR029462, Rnk_N IPR001437, Tscrpt_elong_fac_GreA/B_C IPR023459, Tscrpt_elong_fac_GreA/B_fam |
PANTHERi | PTHR30437, PTHR30437, 1 hit |
Pfami | View protein in Pfam PF01272, GreA_GreB, 1 hit PF14760, Rnk_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNK_ECOLI | |
Accessioni | P0AFW4Primary (citable) accession number: P0AFW4 Secondary accession number(s): P40679 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families