UniProtKB - P0AFV4 (MEPS_ECOLI)
Protein
Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase
Gene
mepS
Organism
Escherichia coli (strain K12)
Status
Functioni
A murein DD-endopeptidase with specificity for D-Ala-meso-diaminopimelic acid (mDAP) cross-links. Its role is probably to cleave D-Ala-mDAP cross-links to allow insertion of new glycans and thus cell wall expansion. Functionally redundant with MepM and MepH. Also has weak LD-carboxypeptidase activity on L-mDAP-D-Ala peptide bonds. Partially suppresses a prc disruption mutant.2 Publications
Catalytic activityi
- H2O + N-acetyl-D-glucosaminyl-N-acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanine = D-alanine + N-acetyl-D-glucosaminyl-N-acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysylEC:3.4.17.13
: cell wall polysaccharide biosynthesis Pathwayi
This protein is involved in the pathway cell wall polysaccharide biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway cell wall polysaccharide biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 94 | NucleophileCurated | 1 | |
Active sitei | 145 | Proton acceptorCurated | 1 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB-KW
- endopeptidase activity Source: EcoCyc
GO - Biological processi
- capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
- cell wall organization Source: UniProtKB-KW
- peptidoglycan metabolic process Source: EcoCyc
- peptidoglycan turnover Source: CACAO
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BioCyci | EcoCyc:G7147-MONOMER ECOL316407:JW2163-MONOMER MetaCyc:G7147-MONOMER |
UniPathwayi | UPA00963 |
Protein family/group databases
MEROPSi | C40.004 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mepS Synonyms:spr, yeiV Ordered Locus Names:b2175, JW2163 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell outer membrane Curated; Lipid-anchor PROSITE-ProRule annotation
GO - Cellular componenti
- cell outer membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell outer membrane, MembranePathology & Biotechi
Disruption phenotypei
Unable to grow on nutrient agar at 42 degrees Celsius. A triple mepS-mepH-mepM mutant is inviable, whereas a double mepS-mepM will grow on a nutrient-poor medium but not on a rich medium, suggesting the 3 endopeptidases are functionally redundant in vivo. Depletion experiments of the double or triple mutants lead to cell lysis, as well as significantly decreased incorporation of mDAP into peptidogylcan sacculi and increased amounts of the enzyme's substrate (Tetra-Tetra-anhydro muropeptide).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 94 | C → A: Loss of DD-endopeptidase activity, no complementation of double mepS-mepM deletion mutants. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | PROSITE-ProRule annotationAdd BLAST | 26 | |
ChainiPRO_0000019763 | 27 – 188 | Murein DD-endopeptidase MepS/Murein LD-carboxypeptidaseAdd BLAST | 162 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 27 | N-palmitoyl cysteinePROSITE-ProRule annotation | 1 | |
Lipidationi | 27 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
PaxDbi | P0AFV4 |
PRIDEi | P0AFV4 |
Interactioni
Subunit structurei
Monomer.
CuratedProtein-protein interaction databases
BioGridi | 4261093, 195 interactors |
IntActi | P0AFV4, 8 interactors |
STRINGi | 511145.b2175 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFV4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFV4 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C40 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG4107TT5 Bacteria COG0791 LUCA |
HOGENOMi | HOG000229978 |
InParanoidi | P0AFV4 |
KOi | K13694 |
PhylomeDBi | P0AFV4 |
Family and domain databases
InterProi | View protein in InterPro IPR000064 NLP_P60_dom IPR038765 Papain-like_cys_pep_sf |
Pfami | View protein in Pfam PF00877 NLPC_P60, 1 hit |
SUPFAMi | SSF54001 SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS51257 PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AFV4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVKSQPILRY ILRGIPAIAV AVLLSACSAN NTAKNMHPET RAVGSETSSL
60 70 80 90 100
QASQDEFENL VRNVDVKSRI MDQYADWKGV RYRLGGSTKK GIDCSGFVQR
110 120 130 140 150
TFREQFGLEL PRSTYEQQEM GKSVSRSNLR TGDLVLFRAG STGRHVGIYI
160 170 180
GNNQFVHAST SSGVIISSMN EPYWKKRYNE ARRVLSRS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86610 Genomic DNA Translation: BAA13140.1 U00096 Genomic DNA Translation: AAC75236.1 AP009048 Genomic DNA Translation: BAA15983.1 |
PIRi | F64986 |
RefSeqi | NP_416680.1, NC_000913.3 WP_000241011.1, NZ_STEB01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC75236; AAC75236; b2175 BAA15983; BAA15983; BAA15983 |
GeneIDi | 946686 |
KEGGi | ecj:JW2163 eco:b2175 |
PATRICi | fig|511145.12.peg.2263 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D86610 Genomic DNA Translation: BAA13140.1 U00096 Genomic DNA Translation: AAC75236.1 AP009048 Genomic DNA Translation: BAA15983.1 |
PIRi | F64986 |
RefSeqi | NP_416680.1, NC_000913.3 WP_000241011.1, NZ_STEB01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2K1G | NMR | - | A | 63-188 | [»] | |
SMRi | P0AFV4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 4261093, 195 interactors |
IntActi | P0AFV4, 8 interactors |
STRINGi | 511145.b2175 |
Protein family/group databases
MEROPSi | C40.004 |
Proteomic databases
PaxDbi | P0AFV4 |
PRIDEi | P0AFV4 |
Genome annotation databases
EnsemblBacteriai | AAC75236; AAC75236; b2175 BAA15983; BAA15983; BAA15983 |
GeneIDi | 946686 |
KEGGi | ecj:JW2163 eco:b2175 |
PATRICi | fig|511145.12.peg.2263 |
Organism-specific databases
EchoBASEi | EB3829 |
Phylogenomic databases
eggNOGi | ENOG4107TT5 Bacteria COG0791 LUCA |
HOGENOMi | HOG000229978 |
InParanoidi | P0AFV4 |
KOi | K13694 |
PhylomeDBi | P0AFV4 |
Enzyme and pathway databases
UniPathwayi | UPA00963 |
BioCyci | EcoCyc:G7147-MONOMER ECOL316407:JW2163-MONOMER MetaCyc:G7147-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFV4 |
PROi | PR:P0AFV4 |
Family and domain databases
InterProi | View protein in InterPro IPR000064 NLP_P60_dom IPR038765 Papain-like_cys_pep_sf |
Pfami | View protein in Pfam PF00877 NLPC_P60, 1 hit |
SUPFAMi | SSF54001 SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS51257 PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MEPS_ECOLI | |
Accessioni | P0AFV4Primary (citable) accession number: P0AFV4 Secondary accession number(s): O08016, P77685 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | November 13, 2019 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references