UniProtKB - P0AFT5 (BTSR_ECOLI)
Protein
Transcriptional regulatory protein BtsR
Gene
btsR
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system BtsS/BtsR, which is part of a nutrient-sensing regulatory network composed of BtsS/BtsR, the low-affinity pyruvate signaling system YpdA/YpdB and their respective target proteins, BtsT and YhjX. Responds to depletion of nutrients, specifically serine, and the concomitant presence of extracellular pyruvate. BtsR regulates expression of btsT by binding to its promoter region. Activation of the BtsS/BtsR signaling cascade also suppresses YpdA/YpdB-mediated yhjX induction.4 Publications
GO - Molecular functioni
- DNA-binding transcription activator activity Source: GO_Central
- phosphorelay response regulator activity Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- phosphorelay signal transduction system Source: EcoCyc
- regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation, Two-component regulatory system |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12006-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Transcriptional regulatory protein BtsRCurated |
Gene namesi | Name:btsR1 Publication Synonyms:yehT Ordered Locus Names:b2125, JW5352 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Other locations
- protein-DNA complex Source: GO_Central
Pathology & Biotechi
Disruption phenotypei
Deletion of btsSR has no obvious phenotypic effect under the conditions tested.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 54 | D → E: 2-fold increase in btsT induction. 1 Publication | 1 | |
Mutagenesisi | 54 | D → N: 10-fold decrease in btsT induction. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081361 | 1 – 239 | Transcriptional regulatory protein BtsRAdd BLAST | 239 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 54 | 4-aspartylphosphatePROSITE-ProRule annotation | 1 |
Post-translational modificationi
Phosphorylated by BtsS.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AFT5 |
PaxDbi | P0AFT5 |
PRIDEi | P0AFT5 |
Interactioni
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4261849, 319 interactors 853268, 4 interactors |
DIPi | DIP-48085N |
IntActi | P0AFT5, 3 interactors |
STRINGi | 511145.b2125 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 116 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 114 | |
Domaini | 137 – 239 | HTH LytTR-typePROSITE-ProRule annotationAdd BLAST | 103 |
Phylogenomic databases
eggNOGi | COG3279, Bacteria |
HOGENOMi | CLU_000445_14_1_6 |
InParanoidi | P0AFT5 |
PhylomeDBi | P0AFT5 |
Family and domain databases
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR007492, LytTR_DNA-bd_dom IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like |
PANTHERi | PTHR48111, PTHR48111, 1 hit |
Pfami | View protein in Pfam PF04397, LytTR, 1 hit PF00072, Response_reg, 1 hit |
SMARTi | View protein in SMART SM00850, LytTR, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS50930, HTH_LYTTR, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFT5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKVLIVDDE PLARENLRVF LQEQSDIEIV GECSNAVEGI GAVHKLRPDV
60 70 80 90 100
LFLDIQMPRI SGLEMVGMLD PEHRPYIVFL TAFDEYAIKA FEEHAFDYLL
110 120 130 140 150
KPIDEARLEK TLARLRQERS KQDVSLLPEN QQALKFIPCT GHSRIYLLQM
160 170 180 190 200
KDVAFVSSRM SGVYVTSHEG KEGFTELTLR TLESRTPLLR CHRQYLVNLA
210 220 230
HLQEIRLEDN GQAELILRNG LTVPVSRRYL KSLKEAIGL
Sequence cautioni
The sequence AAA60488 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60488.1 Different initiation. U00096 Genomic DNA Translation: AAC75186.2 AP009048 Genomic DNA Translation: BAE76601.1 |
PIRi | D64980 |
RefSeqi | NP_416629.4, NC_000913.3 WP_000598641.1, NZ_STEB01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC75186; AAC75186; b2125 BAE76601; BAE76601; BAE76601 |
GeneIDi | 58463038 949024 |
KEGGi | ecj:JW5352 eco:b2125 |
PATRICi | fig|1411691.4.peg.120 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60488.1 Different initiation. U00096 Genomic DNA Translation: AAC75186.2 AP009048 Genomic DNA Translation: BAE76601.1 |
PIRi | D64980 |
RefSeqi | NP_416629.4, NC_000913.3 WP_000598641.1, NZ_STEB01000002.1 |
3D structure databases
SMRi | P0AFT5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261849, 319 interactors 853268, 4 interactors |
DIPi | DIP-48085N |
IntActi | P0AFT5, 3 interactors |
STRINGi | 511145.b2125 |
Proteomic databases
jPOSTi | P0AFT5 |
PaxDbi | P0AFT5 |
PRIDEi | P0AFT5 |
Genome annotation databases
EnsemblBacteriai | AAC75186; AAC75186; b2125 BAE76601; BAE76601; BAE76601 |
GeneIDi | 58463038 949024 |
KEGGi | ecj:JW5352 eco:b2125 |
PATRICi | fig|1411691.4.peg.120 |
Organism-specific databases
EchoBASEi | EB1944 |
Phylogenomic databases
eggNOGi | COG3279, Bacteria |
HOGENOMi | CLU_000445_14_1_6 |
InParanoidi | P0AFT5 |
PhylomeDBi | P0AFT5 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12006-MONOMER |
Miscellaneous databases
PROi | PR:P0AFT5 |
Family and domain databases
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR007492, LytTR_DNA-bd_dom IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like |
PANTHERi | PTHR48111, PTHR48111, 1 hit |
Pfami | View protein in Pfam PF04397, LytTR, 1 hit PF00072, Response_reg, 1 hit |
SMARTi | View protein in SMART SM00850, LytTR, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
PROSITEi | View protein in PROSITE PS50930, HTH_LYTTR, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BTSR_ECOLI | |
Accessioni | P0AFT5Primary (citable) accession number: P0AFT5 Secondary accession number(s): P33356 Q9ALR8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |