btsR

Functionⁱ

Member of the two-component regulatory system BtsS/BtsR, which is part of a nutrient-sensing regulatory network composed of BtsS/BtsR, the low-affinity pyruvate signaling system YpdA/YpdB and their respective target proteins, BtsT and YhjX. Responds to depletion of nutrients, specifically serine, and the concomitant presence of extracellular pyruvate. BtsR regulates expression of btsT by binding to its promoter region. Activation of the BtsS/BtsR signaling cascade also suppresses YpdA/YpdB-mediated yhjX induction.4 Publications

GO - Molecular functionⁱ

DNA binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

phosphorelay signal transduction system
Source: EcoCyc
Inferred from direct assayⁱ
- 22685278
regulation of transcription, DNA-templated
Source: EcoCyc
Inferred from direct assayⁱ
- 22685278
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation, Two-component regulatory system

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG12006-MONOMER

BioCycⁱ

EcoCyc:EG12006-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcriptional regulatory protein BtsRCurated
Gene namesⁱ	Name:btsR1 Publication Synonyms:yehT Ordered Locus Names:b2125, JW5352
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG12006 yehT

EcoGeneⁱ

EG12006 yehT

Subcellular locationⁱ

GO - Cellular componentⁱ

intracellular Source: GOC

View the complete GO annotation on QuickGO ...

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Deletion of btsSR has no obvious phenotypic effect under the conditions tested.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	54	D → E: 2-fold increase in btsT induction. 1 Publication		1
Mutagenesisⁱ	54	D → N: 10-fold decrease in btsT induction. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000081361	1 – 239	Transcriptional regulatory protein BtsRAdd BLAST		239

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	54	4-aspartylphosphatePROSITE-ProRule annotation		1

Post-translational modificationⁱ

Phosphorylated by BtsS.1 Publication

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	P0AFT5
PRIDEⁱ	P0AFT5

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
proA	P07004	4	EBI-556431,EBI-548584
sfsA	P0A823	2	EBI-556431,EBI-556413

Protein-protein interaction databases

BioGridⁱ	4261849, 319 interactors
Database of interacting proteins More...DIPⁱ	DIP-48085N
IntActⁱ	P0AFT5, 3 interactors
STRINGⁱ	316385.ECDH10B_2280

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0AFT5
SMRⁱ	P0AFT5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	3 – 116	Response regulatoryPROSITE-ProRule annotationAdd BLAST		114
Domainⁱ	137 – 239	HTH LytTR-typePROSITE-ProRule annotationAdd BLAST		103

Phylogenomic databases

eggNOGⁱ	ENOG4108NEV Bacteria COG3279 LUCA
HOGENOMⁱ	HOG000242207
InParanoidⁱ	P0AFT5
KEGG Orthology (KO) More...KOⁱ	K02477
OMAⁱ	VVGECAN
PhylomeDBⁱ	P0AFT5

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00156 REC, 1 hit
InterProⁱ	View protein in InterPro IPR011006 CheY-like_superfamily IPR007492 LytTR_DNA-bd_dom IPR001789 Sig_transdc_resp-reg_receiver
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04397 LytTR, 1 hit PF00072 Response_reg, 1 hit
SMARTⁱ	View protein in SMART SM00850 LytTR, 1 hit SM00448 REC, 1 hit
SUPFAMⁱ	SSF52172 SSF52172, 1 hit
PROSITEⁱ	View protein in PROSITE PS50930 HTH_LYTTR, 1 hit PS50110 RESPONSE_REGULATORY, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AFT5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIKVLIVDDE PLARENLRVF LQEQSDIEIV GECSNAVEGI GAVHKLRPDV 
        60         70         80         90        100
LFLDIQMPRI SGLEMVGMLD PEHRPYIVFL TAFDEYAIKA FEEHAFDYLL 
       110        120        130        140        150
KPIDEARLEK TLARLRQERS KQDVSLLPEN QQALKFIPCT GHSRIYLLQM 
       160        170        180        190        200
KDVAFVSSRM SGVYVTSHEG KEGFTELTLR TLESRTPLLR CHRQYLVNLA 
       210        220        230 
HLQEIRLEDN GQAELILRNG LTVPVSRRYL KSLKEAIGL

Length:239

Mass (Da):27,400

Last modified:December 20, 2005 - v1

Checksum:ⁱ5665AAE782B7FBA3

GO

Sequence cautionⁱ

The sequence AAA60488 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00007 Genomic DNA Translation: AAA60488.1 Different initiation. U00096 Genomic DNA Translation: AAC75186.2 AP009048 Genomic DNA Translation: BAE76601.1
PIRⁱ	D64980
NCBI Reference Sequences More...RefSeqⁱ	NP_416629.4, NC_000913.3 WP_000598641.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC75186; AAC75186; b2125 BAE76601; BAE76601; BAE76601
GeneIDⁱ	949024
KEGGⁱ	ecj:JW5352 eco:b2125
PATRICⁱ	fig\|1411691.4.peg.120

Protein	Similar proteins		Species	Score	Length	Source
P0AFT5	Transcriptional regulatory protein BtsR		ECO57		239	UniRef100_P0AFT7
Transcriptional regulatory protein BtsR		ECOL6		239
Uncharacterized protein		ECOLX		244
Uncharacterized protein		Escherichia coli D6-113.11		244
Predicted response regulator in two-component system with YehU		Escherichia coli O55:H7		244
+159

Protein	Similar proteins		Species	Score	Length	Source
P0AFT5	Transcriptional regulatory protein BtsR		ECO57		239	UniRef90_P0AFT7
Transcriptional regulatory protein BtsR		ECOL6		239
Transcriptional regulatory protein BtsR		SHIFL		239
Transcriptional regulatory protein BtsR		SALTI		239
Transcriptional regulatory protein BtsR		SALTY		239
+539

Protein	Similar proteins		Species	Score	Length	Source
P0AFT5	Transcriptional regulatory protein BtsR		ECO57		239	UniRef50_P0AFT7
Transcriptional regulatory protein BtsR		ECOL6		239
Transcriptional regulatory protein BtsR		SHIFL		239
Transcriptional regulatory protein BtsR		SALTY		239
Transcriptional regulatory protein BtsR		SALTI		239
+1824

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00007 Genomic DNA Translation: AAA60488.1 Different initiation. U00096 Genomic DNA Translation: AAC75186.2 AP009048 Genomic DNA Translation: BAE76601.1
PIRⁱ	D64980
RefSeqⁱ	NP_416629.4, NC_000913.3 WP_000598641.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P0AFT5
SMRⁱ	P0AFT5
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261849, 319 interactors
DIPⁱ	DIP-48085N
IntActⁱ	P0AFT5, 3 interactors
STRINGⁱ	316385.ECDH10B_2280

Proteomic databases

PaxDbⁱ	P0AFT5
PRIDEⁱ	P0AFT5

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC75186; AAC75186; b2125 BAE76601; BAE76601; BAE76601
GeneIDⁱ	949024
KEGGⁱ	ecj:JW5352 eco:b2125
PATRICⁱ	fig\|1411691.4.peg.120

Organism-specific databases

EchoBASEⁱ	EB1944
EcoGeneⁱ	EG12006 yehT

Phylogenomic databases

eggNOGⁱ	ENOG4108NEV Bacteria COG3279 LUCA
HOGENOMⁱ	HOG000242207
InParanoidⁱ	P0AFT5
KOⁱ	K02477
OMAⁱ	VVGECAN
PhylomeDBⁱ	P0AFT5

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG12006-MONOMER

BioCycⁱ

EcoCyc:EG12006-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0AFT5

PROⁱ

PR:P0AFT5

Family and domain databases

CDDⁱ	cd00156 REC, 1 hit
InterProⁱ	View protein in InterPro IPR011006 CheY-like_superfamily IPR007492 LytTR_DNA-bd_dom IPR001789 Sig_transdc_resp-reg_receiver
Pfamⁱ	View protein in Pfam PF04397 LytTR, 1 hit PF00072 Response_reg, 1 hit
SMARTⁱ	View protein in SMART SM00850 LytTR, 1 hit SM00448 REC, 1 hit
SUPFAMⁱ	SSF52172 SSF52172, 1 hit
PROSITEⁱ	View protein in PROSITE PS50930 HTH_LYTTR, 1 hit PS50110 RESPONSE_REGULATORY, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BTSR_ECOLI
Accessionⁱ	P0AFT5Primary (citable) accession number: P0AFT5 Secondary accession number(s): P33356 , P76432, P76433, Q2MAV5, Q9ALR8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
	Last sequence update:	December 20, 2005
	Last modified:	March 28, 2018
	This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

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UniParc

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UniRef

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Supporting data

UniProtKB - P0AFT5 (BTSR_ECOLI)

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Transcriptional regulatory protein BtsR

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ