UniProtKB - P0AFT2 (TCYL_ECOLI)
L-cystine transport system permease protein TcyL
tcyL
Functioni
Part of the ABC transporter complex TcyJLN involved in L-cystine import (PubMed:25139244, PubMed:20351115, PubMed:25837721, PubMed:26350134).
This high affinity cystine transporter is involved in resistance to oxidative stress by forming a L-cysteine/L-cystine shuttle system with the EamA transporter, which exports L-cysteine as reducing equivalents to the periplasm to prevent the cells from oxidative stress. Exported L-cysteine can reduce the periplasmic hydrogen peroxide to water, and then generated L-cystine is imported back into the cytoplasm via the TcyJLN complex (PubMed:20351115, PubMed:25837721).
Functions at low cystine concentrations (PubMed:26350134).
The system can also transport L-cysteine, diaminopimelic acid (DAP), djenkolate, lanthionine, D-cystine, homocystine, and it mediates accumulation of the toxic compounds L-selenaproline (SCA) and L-selenocystine (SeCys) (PubMed:25139244, PubMed:26350134).
Responsible for the translocation of the substrate across the membrane (Probable).
Curated4 PublicationsActivity regulationi
GO - Molecular functioni
- L-cystine transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- amino acid transport Source: GO_Central
- L-cystine transport Source: EcoCyc
- sulfur utilization Source: EcoCyc
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:G7037-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.3.10, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: L-cystine transport system permease protein TcyLCurated |
Gene namesi | Name:tcyL1 Publication Synonyms:yecS Ordered Locus Names:b1918, JW1903 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- ATP-binding cassette (ABC) transporter complex Source: InterPro
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 22 | PeriplasmicCuratedAdd BLAST | 22 | |
Transmembranei | 23 – 43 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 44 – 64 | CytoplasmicCuratedAdd BLAST | 21 | |
Transmembranei | 65 – 85 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 86 – 182 | PeriplasmicCuratedAdd BLAST | 97 | |
Transmembranei | 183 – 203 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 204 – 222 | Cytoplasmic1 PublicationAdd BLAST | 19 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060250 | 1 – 222 | L-cystine transport system permease protein TcyLAdd BLAST | 222 |
Proteomic databases
jPOSTi | P0AFT2 |
PaxDbi | P0AFT2 |
PRIDEi | P0AFT2 |
Expressioni
Inductioni
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (TcyN), two transmembrane proteins (TcyL) and a solute-binding protein (TcyJ).
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261651, 23 interactors |
ComplexPortali | CPX-4406, L-cystine ABC transporter complex |
IntActi | P0AFT2, 1 interactor |
STRINGi | 511145.b1918 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 207 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 189 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0765, Bacteria |
HOGENOMi | CLU_019602_1_4_6 |
InParanoidi | P0AFT2 |
PhylomeDBi | P0AFT2 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR010065, AA_ABC_transptr_permease_3TM IPR043429, ArtM/GltK/GlnP/TcyL/YhdX-like IPR000515, MetI-like IPR035906, MetI-like_sf |
PANTHERi | PTHR30614, PTHR30614, 1 hit |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
TIGRFAMsi | TIGR01726, HEQRo_perm_3TM, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MQESIQLVID SLPFLLKGAG YTLQLSIGGM FFGLLLGFIL ALMRLSPIWP
60 70 80 90 100
VRWLARFYIS IFRGTPLIAQ LFMIYYGLPQ FGIELDPIPS AMIGLSLNTA
110 120 130 140 150
AYAAETLRAA ISSIDKGQWE AAASIGMTPW QTMRRAILPQ AARVALPPLS
160 170 180 190 200
NSFISLVKDT SLAATIQVPE LFRQAQLITS RTLEVFTMYL AASLIYWIMA
210 220
TVLSTLQNHF ENQLNRQERE PK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74985.1 AP009048 Genomic DNA Translation: BAA15738.1 |
PIRi | C64955 |
RefSeqi | NP_416428.1, NC_000913.3 WP_001158220.1, NZ_STEB01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC74985; AAC74985; b1918 BAA15738; BAA15738; BAA15738 |
GeneIDi | 66674192 949105 |
KEGGi | ecj:JW1903 eco:b1918 |
PATRICi | fig|1411691.4.peg.331 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74985.1 AP009048 Genomic DNA Translation: BAA15738.1 |
PIRi | C64955 |
RefSeqi | NP_416428.1, NC_000913.3 WP_001158220.1, NZ_STEB01000026.1 |
3D structure databases
SMRi | P0AFT2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261651, 23 interactors |
ComplexPortali | CPX-4406, L-cystine ABC transporter complex |
IntActi | P0AFT2, 1 interactor |
STRINGi | 511145.b1918 |
Protein family/group databases
TCDBi | 3.A.1.3.10, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P0AFT2 |
PaxDbi | P0AFT2 |
PRIDEi | P0AFT2 |
Genome annotation databases
EnsemblBacteriai | AAC74985; AAC74985; b1918 BAA15738; BAA15738; BAA15738 |
GeneIDi | 66674192 949105 |
KEGGi | ecj:JW1903 eco:b1918 |
PATRICi | fig|1411691.4.peg.331 |
Organism-specific databases
EchoBASEi | EB3791 |
Phylogenomic databases
eggNOGi | COG0765, Bacteria |
HOGENOMi | CLU_019602_1_4_6 |
InParanoidi | P0AFT2 |
PhylomeDBi | P0AFT2 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7037-MONOMER |
Miscellaneous databases
PROi | PR:P0AFT2 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR010065, AA_ABC_transptr_permease_3TM IPR043429, ArtM/GltK/GlnP/TcyL/YhdX-like IPR000515, MetI-like IPR035906, MetI-like_sf |
PANTHERi | PTHR30614, PTHR30614, 1 hit |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
TIGRFAMsi | TIGR01726, HEQRo_perm_3TM, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TCYL_ECOLI | |
Accessioni | P0AFT2Primary (citable) accession number: P0AFT2 Secondary accession number(s): O07985, P76315 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 23, 2022 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families