UniProtKB - P0AFS9 (MEPM_ECOLI)
Protein
Murein DD-endopeptidase MepM
Gene
mepM
Organism
Escherichia coli (strain K12)
Status
Functioni
A murein DD-endopeptidase with specificity for D-Ala-meso-diaminopimelic acid (mDAP) cross-links. Its role is probably to cleave D-Ala-mDAP cross-links to allow insertion of new glycans and thus cell wall expansion. Functionally redundant with MepM and MepH. Partially suppresses an mepS disruption mutant.1 Publication
Cofactori
Zn2+1 Publication, Ca2+1 PublicationNote: Zinc, although calcium also allows some activity.1 Publication
: cell wall polysaccharide biosynthesis Pathwayi
This protein is involved in the pathway cell wall polysaccharide biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway cell wall polysaccharide biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 314 | ZincSequence analysis | 1 |
GO - Molecular functioni
- endopeptidase activity Source: CACAO
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: EcoCyc
GO - Biological processi
- capsule polysaccharide biosynthetic process Source: UniProtKB-UniPathway
- cell wall organization Source: UniProtKB-KW
- peptidoglycan metabolic process Source: EcoCyc
- peptidoglycan turnover Source: CACAO
- septum digestion after cytokinesis Source: EcoliWiki
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Cell wall biogenesis/degradation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10013-MONOMER MetaCyc:EG10013-MONOMER |
UniPathwayi | UPA00963 |
Names & Taxonomyi
Protein namesi | Recommended name: Murein DD-endopeptidase MepM (EC:3.4.24.-)Alternative name(s): Murein hydrolase MepM ORFU |
Gene namesi | Name:mepM Synonyms:yebA Ordered Locus Names:b1856, JW5304 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane Curated; Single-pass membrane protein Curated Note: Uniform peripheral location, with partial enrichment at cell poles.
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 20 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 21 – 40 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 41 – 440 | PeriplasmicSequence analysisAdd BLAST | 400 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells are shorter in a single mutant, while triple envC-nlpD-mepM disruptions have defects in septation and cell separation and form long filaments (15-fold longer) and further yet by the quadruple disruption mutant (envC-nlpD-mepM-ygeR, over 21-fold longer). Quadruple mutants are less sensitive to ampicillin lysis (PubMed:19525345). A triple mepS-mepH-mepM mutant is inviable, whereas a double mepS-mepM will grow on a nutrient-poor medium but not on a rich medium, suggesting the 3 endopeptidases are functionally redundant in vivo. Depletion experiments of the double or triple mutants lead to cell lysis, as well as significantly decreased incorporation of mDAP into peptidogylcan sacculi and increased amounts of the enzyme's substrate (Tetra-Tetra-anhydro muropeptide) (PubMed:23062283).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000026826 | 1 – 440 | Murein DD-endopeptidase MepMAdd BLAST | 440 |
Proteomic databases
PaxDbi | P0AFS9 |
PRIDEi | P0AFS9 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260352, 385 interactors |
STRINGi | 511145.b1856 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 96 – 141 | LysMPROSITE-ProRule annotationAdd BLAST | 46 |
Sequence similaritiesi
Belongs to the peptidase M23B family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0739, Bacteria |
HOGENOMi | CLU_026846_0_2_6 |
InParanoidi | P0AFS9 |
PhylomeDBi | P0AFS9 |
Family and domain databases
CDDi | cd00118, LysM, 1 hit |
Gene3Di | 2.70.70.10, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR018392, LysM_dom IPR013731, OapA_N IPR016047, Peptidase_M23 |
Pfami | View protein in Pfam PF01476, LysM, 1 hit PF08525, OapA_N, 1 hit PF01551, Peptidase_M23, 1 hit |
SMARTi | View protein in SMART SM00257, LysM, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit |
PROSITEi | View protein in PROSITE PS51782, LYSM, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFS9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQQIARSVAL AFNNLPRPHR VMLGSLTVLT LAVAVWRPYV YHRDATPIVK
60 70 80 90 100
TIELEQNEIR SLLPEASEPI DQAAQEDEAI PQDELDDKIA GEAGVHEYVV
110 120 130 140 150
STGDTLSSIL NQYGIDMGDI TQLAAADKEL RNLKIGQQLS WTLTADGELQ
160 170 180 190 200
RLTWEVSRRE TRTYDRTAAN GFKMTSEMQQ GEWVNNLLKG TVGGSFVASA
210 220 230 240 250
RNAGLTSAEV SAVIKAMQWQ MDFRKLKKGD EFAVLMSREM LDGKREQSQL
260 270 280 290 300
LGVRLRSEGK DYYAIRAEDG KFYDRNGTGL AKGFLRFPTA KQFRISSNFN
310 320 330 340 350
PRRTNPVTGR VAPHRGVDFA MPQGTPVLSV GDGEVVVAKR SGAAGYYVAI
360 370 380 390 400
RHGRSYTTRY MHLRKILVKP GQKVKRGDRI ALSGNTGRST GPHLHYEVWI
410 420 430 440
NQQAVNPLTA KLPRTEGLTG SDRREFLAQA KEIVPQLRFD
Sequence cautioni
The sequence AAA81029 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 90 | A → P in AAA81029 (Ref. 4) Curated | 1 | |
Sequence conflicti | 93 | A → T in AAA81029 (Ref. 4) Curated | 1 | |
Sequence conflicti | 121 | T → S in AAA81029 (Ref. 4) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74926.2 AP009048 Genomic DNA Translation: BAA15664.2 U38702 Genomic DNA Translation: AAA81029.1 Different initiation. M77039 Genomic DNA Translation: AAA24180.1 |
RefSeqi | NP_416370.2, NC_000913.3 WP_001184045.1, NZ_STEB01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC74926; AAC74926; b1856 BAA15664; BAA15664; BAA15664 |
GeneIDi | 49584393 946376 |
KEGGi | ecj:JW5304 eco:b1856 |
PATRICi | fig|1411691.4.peg.392 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74926.2 AP009048 Genomic DNA Translation: BAA15664.2 U38702 Genomic DNA Translation: AAA81029.1 Different initiation. M77039 Genomic DNA Translation: AAA24180.1 |
RefSeqi | NP_416370.2, NC_000913.3 WP_001184045.1, NZ_STEB01000009.1 |
3D structure databases
SMRi | P0AFS9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260352, 385 interactors |
STRINGi | 511145.b1856 |
Proteomic databases
PaxDbi | P0AFS9 |
PRIDEi | P0AFS9 |
Genome annotation databases
EnsemblBacteriai | AAC74926; AAC74926; b1856 BAA15664; BAA15664; BAA15664 |
GeneIDi | 49584393 946376 |
KEGGi | ecj:JW5304 eco:b1856 |
PATRICi | fig|1411691.4.peg.392 |
Organism-specific databases
EchoBASEi | EB0013 |
Phylogenomic databases
eggNOGi | COG0739, Bacteria |
HOGENOMi | CLU_026846_0_2_6 |
InParanoidi | P0AFS9 |
PhylomeDBi | P0AFS9 |
Enzyme and pathway databases
UniPathwayi | UPA00963 |
BioCyci | EcoCyc:EG10013-MONOMER MetaCyc:EG10013-MONOMER |
Miscellaneous databases
PROi | PR:P0AFS9 |
Family and domain databases
CDDi | cd00118, LysM, 1 hit |
Gene3Di | 2.70.70.10, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR018392, LysM_dom IPR013731, OapA_N IPR016047, Peptidase_M23 |
Pfami | View protein in Pfam PF01476, LysM, 1 hit PF08525, OapA_N, 1 hit PF01551, Peptidase_M23, 1 hit |
SMARTi | View protein in SMART SM00257, LysM, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit |
PROSITEi | View protein in PROSITE PS51782, LYSM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MEPM_ECOLI | |
Accessioni | P0AFS9Primary (citable) accession number: P0AFS9 Secondary accession number(s): O07981, P24204, P76283 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries