UniProtKB - P0AFS5 (TQSA_ECOLI)
Protein
AI-2 transport protein TqsA
Gene
tqsA
Organism
Escherichia coli (strain K12)
Status
Functioni
Controls the transport of the quorum-sensing signal AI-2 either by enhancing its secretion or inhibiting its uptake and consequently represses biofilm formation and motility and affects the global gene expression in biofilms.1 Publication
GO - Molecular functioni
- efflux transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- autoinducer AI-2 transmembrane transport Source: EcoCyc
- quorum sensing Source: EcoCyc
- transmembrane transport Source: GO_Central
Keywordsi
Biological process | Quorum sensing, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:G6859-MONOMER MetaCyc:G6859-MONOMER |
Protein family/group databases
TCDBi | 2.A.86.1.4, the autoinducer-2 exporter (ai-2e) family (formerly the perm family, tc #9,b,22) |
Names & Taxonomyi
Protein namesi | Recommended name: AI-2 transport protein TqsAAlternative name(s): Transport of quorum-sensing signal protein |
Gene namesi | Name:tqsA Synonyms:ydgG Ordered Locus Names:b1601, JW1593 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | PeriplasmicSequence analysis | 4 | |
Transmembranei | 5 – 25 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 26 – 30 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 31 – 51 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 52 – 61 | PeriplasmicSequence analysis | 10 | |
Transmembranei | 62 – 82 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 83 – 149 | CytoplasmicSequence analysisAdd BLAST | 67 | |
Transmembranei | 150 – 170 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 171 – 197 | PeriplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 198 – 218 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 219 – 221 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 222 – 242 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 243 – 257 | PeriplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 258 – 278 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 279 – 292 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 293 – 313 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 314 – 316 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 317 – 337 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 338 – 344 | CytoplasmicSequence analysis | 7 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells have a decreased extracellular and increased intracellular concentration of AI-2. Deletion of tqsA results in a 7000-fold increase in biofilm thickness and 574-fold increase in biomass in flow cells. Deletion of tqsA increases cell motility by increasing transcription of flagellar genes. The tqsA deletion mutant shows higher resistance toward crystal violet, spectinomycin, streptomycin sulfate, 2,6-dichloroquinone-4-chloroimide, chloramphenicol and amoxicillin.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000148297 | 1 – 344 | AI-2 transport protein TqsAAdd BLAST | 344 |
Proteomic databases
PaxDbi | P0AFS5 |
PRIDEi | P0AFS5 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260647, 14 interactors |
STRINGi | 511145.b1601 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0628, Bacteria |
HOGENOMi | CLU_031275_0_3_6 |
InParanoidi | P0AFS5 |
PhylomeDBi | P0AFS5 |
Family and domain databases
InterProi | View protein in InterPro IPR002549, AI-2E-like |
PANTHERi | PTHR21716, PTHR21716, 1 hit |
Pfami | View protein in Pfam PF01594, AI-2E_transport, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFS5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKPIITLNG LKIVIMLGML VIILCGIRFA AEIIVPFILA LFIAVILNPL
60 70 80 90 100
VQHMVRWRVP RVLAVSILMT IIVMAMVLLL AYLGSALNEL TRTLPQYRNS
110 120 130 140 150
IMTPLQALEP LLQRVGIDVS VDQLAHYIDP NAAMTLLTNL LTQLSNAMSS
160 170 180 190 200
IFLLLLTVLF MLLEVPQLPG KFQQMMARPV EGMAAIQRAI DSVSHYLVLK
210 220 230 240 250
TAISIITGLV AWAMLAALDV RFAFVWGLLA FALNYIPNIG SVLAAIPPIA
260 270 280 290 300
QVLVFNGFYE ALLVLAGYLL INLVFGNILE PRIMGRGLGL STLVVFLSLI
310 320 330 340
FWGWLLGPVG MLLSVPLTII VKIALEQTAG GQSIAVLLSD LNKE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74673.1 AP009048 Genomic DNA Translation: BAA15335.1 |
PIRi | C64916 |
RefSeqi | NP_416118.1, NC_000913.3 WP_001118241.1, NZ_STEB01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC74673; AAC74673; b1601 BAA15335; BAA15335; BAA15335 |
GeneIDi | 57730995 946142 |
KEGGi | ecj:JW1593 eco:b1601 |
PATRICi | fig|1411691.4.peg.661 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74673.1 AP009048 Genomic DNA Translation: BAA15335.1 |
PIRi | C64916 |
RefSeqi | NP_416118.1, NC_000913.3 WP_001118241.1, NZ_STEB01000003.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260647, 14 interactors |
STRINGi | 511145.b1601 |
Protein family/group databases
TCDBi | 2.A.86.1.4, the autoinducer-2 exporter (ai-2e) family (formerly the perm family, tc #9,b,22) |
Proteomic databases
PaxDbi | P0AFS5 |
PRIDEi | P0AFS5 |
Genome annotation databases
EnsemblBacteriai | AAC74673; AAC74673; b1601 BAA15335; BAA15335; BAA15335 |
GeneIDi | 57730995 946142 |
KEGGi | ecj:JW1593 eco:b1601 |
PATRICi | fig|1411691.4.peg.661 |
Organism-specific databases
EchoBASEi | EB3687 |
Phylogenomic databases
eggNOGi | COG0628, Bacteria |
HOGENOMi | CLU_031275_0_3_6 |
InParanoidi | P0AFS5 |
PhylomeDBi | P0AFS5 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6859-MONOMER MetaCyc:G6859-MONOMER |
Miscellaneous databases
PROi | PR:P0AFS5 |
Family and domain databases
InterProi | View protein in InterPro IPR002549, AI-2E-like |
PANTHERi | PTHR21716, PTHR21716, 1 hit |
Pfami | View protein in Pfam PF01594, AI-2E_transport, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TQSA_ECOLI | |
Accessioni | P0AFS5Primary (citable) accession number: P0AFS5 Secondary accession number(s): P77535 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - SIMILARITY comments
Index of protein domains and families