UniProtKB - P0AFQ7 (YCFH_ECOLI)
Protein
Uncharacterized metal-dependent hydrolase YcfH
Gene
ycfH
Organism
Escherichia coli (strain K12)
Status
Functioni
Has D-tyrosyl-tRNA deacylase activity in vitro.1 Publication
Cofactori
Co2+1 Publication, Mn2+1 Publication, Ni2+1 PublicationNote: Binds 2 divalent metal cations per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 7 | Divalent metal cation 11 Publication | 1 | |
Metal bindingi | 9 | Divalent metal cation 11 Publication | 1 | |
Metal bindingi | 94 | Divalent metal cation 11 Publication | 1 | |
Metal bindingi | 94 | Divalent metal cation 21 Publication | 1 | |
Metal bindingi | 130 | Divalent metal cation 21 Publication | 1 | |
Metal bindingi | 155 | Divalent metal cation 21 Publication | 1 | |
Metal bindingi | 205 | Divalent metal cation 11 Publication | 1 |
GO - Molecular functioni
- endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12303-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ycfH Ordered Locus Names:b1100, JW1086 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201995 | 1 – 265 | Uncharacterized metal-dependent hydrolase YcfHAdd BLAST | 265 |
Proteomic databases
jPOSTi | P0AFQ7 |
PaxDbi | P0AFQ7 |
PRIDEi | P0AFQ7 |
Interactioni
Binary interactionsi
P0AFQ7
With | #Exp. | IntAct |
---|---|---|
gcd [P15877] | 2 | EBI-560527,EBI-545666 |
Protein-protein interaction databases
BioGRIDi | 4260943, 192 interactors |
DIPi | DIP-48157N |
IntActi | P0AFQ7, 9 interactors |
STRINGi | 511145.b1100 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFQ7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFQ7 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0084, Bacteria |
HOGENOMi | CLU_031506_4_0_6 |
InParanoidi | P0AFQ7 |
PhylomeDBi | P0AFQ7 |
Family and domain databases
CDDi | cd01310, TatD_DNAse, 1 hit |
InterProi | View protein in InterPro IPR018228, DNase_TatD-rel_CS IPR015991, Hydrolase_TatD-type IPR032466, Metal_Hydrolase IPR001130, TatD_family |
Pfami | View protein in Pfam PF01026, TatD_DNase, 1 hit |
PIRSFi | PIRSF005902, DNase_TatD, 1 hit |
SUPFAMi | SSF51556, SSF51556, 1 hit |
TIGRFAMsi | TIGR00010, TIGR00010, 1 hit |
PROSITEi | View protein in PROSITE PS01137, TATD_1, 1 hit PS01091, TATD_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFQ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFLVDSHCHL DGLDYESLHK DVDDVLAKAA ARDVKFCLAV ATTLPGYLHM
60 70 80 90 100
RDLVGERDNV VFSCGVHPLN QNDPYDVEDL RRLAAEEGVV ALGETGLDYY
110 120 130 140 150
YTPETKVRQQ ESFIHHIQIG RELNKPVIVH TRDARADTLA ILREEKVTDC
160 170 180 190 200
GGVLHCFTED RETAGKLLDL GFYISFSGIV TFRNAEQLRD AARYVPLDRL
210 220 230 240 250
LVETDSPYLA PVPHRGKENQ PAMVRDVAEY MAVLKGVAVE ELAQVTTDNF
260
ARLFHIDASR LQSIR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 97 | L → Q (PubMed:8509334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74184.1 AP009048 Genomic DNA Translation: BAA35907.1 L01483 Genomic DNA No translation available. L04577 Genomic DNA No translation available. |
PIRi | A64854 |
RefSeqi | NP_415618.1, NC_000913.3 WP_000480245.1, NZ_STEB01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC74184; AAC74184; b1100 BAA35907; BAA35907; BAA35907 |
GeneIDi | 58461220 945656 |
KEGGi | ecj:JW1086 eco:b1100 |
PATRICi | fig|1411691.4.peg.1168 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74184.1 AP009048 Genomic DNA Translation: BAA35907.1 L01483 Genomic DNA No translation available. L04577 Genomic DNA No translation available. |
PIRi | A64854 |
RefSeqi | NP_415618.1, NC_000913.3 WP_000480245.1, NZ_STEB01000016.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YIX | X-ray | 1.90 | A/B | 1-265 | [»] | |
SMRi | P0AFQ7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260943, 192 interactors |
DIPi | DIP-48157N |
IntActi | P0AFQ7, 9 interactors |
STRINGi | 511145.b1100 |
Proteomic databases
jPOSTi | P0AFQ7 |
PaxDbi | P0AFQ7 |
PRIDEi | P0AFQ7 |
Genome annotation databases
EnsemblBacteriai | AAC74184; AAC74184; b1100 BAA35907; BAA35907; BAA35907 |
GeneIDi | 58461220 945656 |
KEGGi | ecj:JW1086 eco:b1100 |
PATRICi | fig|1411691.4.peg.1168 |
Organism-specific databases
EchoBASEi | EB2209 |
Phylogenomic databases
eggNOGi | COG0084, Bacteria |
HOGENOMi | CLU_031506_4_0_6 |
InParanoidi | P0AFQ7 |
PhylomeDBi | P0AFQ7 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12303-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFQ7 |
PROi | PR:P0AFQ7 |
Family and domain databases
CDDi | cd01310, TatD_DNAse, 1 hit |
InterProi | View protein in InterPro IPR018228, DNase_TatD-rel_CS IPR015991, Hydrolase_TatD-type IPR032466, Metal_Hydrolase IPR001130, TatD_family |
Pfami | View protein in Pfam PF01026, TatD_DNase, 1 hit |
PIRSFi | PIRSF005902, DNase_TatD, 1 hit |
SUPFAMi | SSF51556, SSF51556, 1 hit |
TIGRFAMsi | TIGR00010, TIGR00010, 1 hit |
PROSITEi | View protein in PROSITE PS01137, TATD_1, 1 hit PS01091, TATD_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YCFH_ECOLI | |
Accessioni | P0AFQ7Primary (citable) accession number: P0AFQ7 Secondary accession number(s): P37346, P78057 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families