The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P0AFL3 (PPIA_ECOLI)
Protein
Peptidyl-prolyl cis-trans isomerase A
Gene
ppiA
Organism
Escherichia coli (strain K12)
Status
Functioni
PPIases accelerate the folding of proteins (Probable). It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed:2190212).
Curated1 PublicationCatalytic activityi
- EC:5.2.1.81 Publication
Activity regulationi
Inhibition by cyclosporin A with a Ki of 25 to 50 mu-mol, a concentration 1000-fold higher than that required for eukaryotic PPIases.1 Publication
GO - Molecular functioni
- peptidyl-prolyl cis-trans isomerase activity Source: EcoCyc
GO - Biological processi
- protein folding Source: InterPro
- protein peptidyl-prolyl isomerization Source: EcoCyc
Keywordsi
Molecular function | Isomerase, Rotamase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10757-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Peptidyl-prolyl cis-trans isomerase A1 Publication (EC:5.2.1.81 Publication)Short name: PPIase A1 Publication Alternative name(s): Cyclophilin A Rotamase A1 Publication |
Gene namesi | Name:ppiA Synonyms:rot, rotA Ordered Locus Names:b3363, JW3326 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm 1 Publication
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
- periplasmic space Source: EcoliWiki
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 136 | F → W: Enhances susceptibility to CSA inhibition. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB08168, Coumarin 120 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | 1 PublicationAdd BLAST | 24 | |
ChainiPRO_0000025497 | 25 – 190 | Peptidyl-prolyl cis-trans isomerase AAdd BLAST | 166 |
Proteomic databases
jPOSTi | P0AFL3 |
PaxDbi | P0AFL3 |
PRIDEi | P0AFL3 |
2D gel databases
SWISS-2DPAGEi | P0AFL3 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261085, 160 interactors |
DIPi | DIP-48080N |
IntActi | P0AFL3, 1 interactor |
STRINGi | 511145.b3363 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0AFL3 |
BMRBi | P0AFL3 |
SMRi | P0AFL3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFL3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 188 | PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST | 162 |
Sequence similaritiesi
Belongs to the cyclophilin-type PPIase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0652, Bacteria |
HOGENOMi | CLU_012062_16_9_6 |
InParanoidi | P0AFL3 |
OMAi | VPFHRVM |
PhylomeDBi | P0AFL3 |
Family and domain databases
Gene3Di | 2.40.100.10, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom IPR044665, E_coli_cyclophilin_A-like |
PANTHERi | PTHR43246, PTHR43246, 1 hit |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SUPFAMi | SSF50891, SSF50891, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AFL3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFKSTLAAMA AVFALSALSP AAMAAKGDPH VLLTTSAGNI ELELDKQKAP
60 70 80 90 100
VSVQNFVDYV NSGFYNNTTF HRVIPGFMIQ GGGFTEQMQQ KKPNPPIKNE
110 120 130 140 150
ADNGLRNTRG TIAMARTADK DSATSQFFIN VADNAFLDHG QRDFGYAVFG
160 170 180 190
KVVKGMDVAD KISQVPTHDV GPYQNVPSKP VVILSAKVLP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55429 Genomic DNA Translation: AAA23451.1 M28363 Genomic DNA Translation: AAA23772.1 U18997 Genomic DNA Translation: AAA58160.1 U00096 Genomic DNA Translation: AAC76388.1 AP009048 Genomic DNA Translation: BAE77927.1 M32354 Genomic DNA Translation: AAA24261.1 |
PIRi | A37964, CSECA |
RefSeqi | NP_417822.1, NC_000913.3 WP_000477225.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76388; AAC76388; b3363 BAE77927; BAE77927; BAE77927 |
GeneIDi | 66672756 947870 |
KEGGi | ecj:JW3326 eco:b3363 |
PATRICi | fig|1411691.4.peg.3367 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55429 Genomic DNA Translation: AAA23451.1 M28363 Genomic DNA Translation: AAA23772.1 U18997 Genomic DNA Translation: AAA58160.1 U00096 Genomic DNA Translation: AAC76388.1 AP009048 Genomic DNA Translation: BAE77927.1 M32354 Genomic DNA Translation: AAA24261.1 |
PIRi | A37964, CSECA |
RefSeqi | NP_417822.1, NC_000913.3 WP_000477225.1, NZ_STEB01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CLH | NMR | - | A | 25-190 | [»] | |
1J2A | X-ray | 1.80 | A | 25-190 | [»] | |
1V9T | X-ray | 1.70 | A/B | 25-190 | [»] | |
1VAI | X-ray | 1.80 | A/B | 25-190 | [»] | |
AlphaFoldDBi | P0AFL3 | |||||
BMRBi | P0AFL3 | |||||
SMRi | P0AFL3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261085, 160 interactors |
DIPi | DIP-48080N |
IntActi | P0AFL3, 1 interactor |
STRINGi | 511145.b3363 |
Chemistry databases
DrugBanki | DB08168, Coumarin 120 |
2D gel databases
SWISS-2DPAGEi | P0AFL3 |
Proteomic databases
jPOSTi | P0AFL3 |
PaxDbi | P0AFL3 |
PRIDEi | P0AFL3 |
Genome annotation databases
EnsemblBacteriai | AAC76388; AAC76388; b3363 BAE77927; BAE77927; BAE77927 |
GeneIDi | 66672756 947870 |
KEGGi | ecj:JW3326 eco:b3363 |
PATRICi | fig|1411691.4.peg.3367 |
Organism-specific databases
EchoBASEi | EB0750 |
Phylogenomic databases
eggNOGi | COG0652, Bacteria |
HOGENOMi | CLU_012062_16_9_6 |
InParanoidi | P0AFL3 |
OMAi | VPFHRVM |
PhylomeDBi | P0AFL3 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10757-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFL3 |
PROi | PR:P0AFL3 |
Family and domain databases
Gene3Di | 2.40.100.10, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom IPR044665, E_coli_cyclophilin_A-like |
PANTHERi | PTHR43246, PTHR43246, 1 hit |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SUPFAMi | SSF50891, SSF50891, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PPIA_ECOLI | |
Accessioni | P0AFL3Primary (citable) accession number: P0AFL3 Secondary accession number(s): P20752, Q2M729 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | May 25, 2022 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families