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Protein

Peptidyl-prolyl cis-trans isomerase A

Gene

ppiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.1 Publication

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

Activity regulationi

Inhibition by cyclosporin A with a Ki of 25 to 50 mu-mol, a concentration 100-fold higher than that required for eukaryotic PPIases.

GO - Molecular functioni

  • peptidyl-prolyl cis-trans isomerase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Rotamase

Enzyme and pathway databases

BioCyciEcoCyc:EG10757-MONOMER
MetaCyc:EG10757-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase A (EC:5.2.1.8)
Short name:
PPIase A
Alternative name(s):
Cyclophilin A
Rotamase A
Gene namesi
Name:ppiA
Synonyms:rot, rotA
Ordered Locus Names:b3363, JW3326
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10757 ppiA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136F → W: Enhances susceptibility to CSA inhibition. 1 Publication1

Chemistry databases

DrugBankiDB08168 7-AMINO-4-METHYL-CHROMEN-2-ONE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000002549725 – 190Peptidyl-prolyl cis-trans isomerase AAdd BLAST166

Proteomic databases

EPDiP0AFL3
PaxDbiP0AFL3
PRIDEiP0AFL3

2D gel databases

SWISS-2DPAGEiP0AFL3

Interactioni

Protein-protein interaction databases

BioGridi4261085, 160 interactors
DIPiDIP-48080N
IntActiP0AFL3, 1 interactor
STRINGi316385.ECDH10B_3539

Structurei

Secondary structure

1190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AFL3
SMRiP0AFL3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFL3

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 188PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST162

Sequence similaritiesi

Belongs to the cyclophilin-type PPIase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108R5K Bacteria
COG0652 LUCA
HOGENOMiHOG000065978
InParanoidiP0AFL3
KOiK03767
OMAiEIFCDEV
PhylomeDBiP0AFL3

Family and domain databases

Gene3Di2.40.100.10, 1 hit
InterProiView protein in InterPro
IPR029000 Cyclophilin-like_dom_sf
IPR024936 Cyclophilin-type_PPIase
IPR020892 Cyclophilin-type_PPIase_CS
IPR002130 Cyclophilin-type_PPIase_dom
PfamiView protein in Pfam
PF00160 Pro_isomerase, 1 hit
PIRSFiPIRSF001467 Peptidylpro_ismrse, 1 hit
PRINTSiPR00153 CSAPPISMRASE
SUPFAMiSSF50891 SSF50891, 1 hit
PROSITEiView protein in PROSITE
PS00170 CSA_PPIASE_1, 1 hit
PS50072 CSA_PPIASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFL3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFKSTLAAMA AVFALSALSP AAMAAKGDPH VLLTTSAGNI ELELDKQKAP
60 70 80 90 100
VSVQNFVDYV NSGFYNNTTF HRVIPGFMIQ GGGFTEQMQQ KKPNPPIKNE
110 120 130 140 150
ADNGLRNTRG TIAMARTADK DSATSQFFIN VADNAFLDHG QRDFGYAVFG
160 170 180 190
KVVKGMDVAD KISQVPTHDV GPYQNVPSKP VVILSAKVLP
Length:190
Mass (Da):20,431
Last modified:December 20, 2005 - v1
Checksum:i8B48535F36AA61A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55429 Genomic DNA Translation: AAA23451.1
M28363 Genomic DNA Translation: AAA23772.1
U18997 Genomic DNA Translation: AAA58160.1
U00096 Genomic DNA Translation: AAC76388.1
AP009048 Genomic DNA Translation: BAE77927.1
M32354 Genomic DNA Translation: AAA24261.1
PIRiA37964 CSECA
RefSeqiNP_417822.1, NC_000913.3
WP_000477225.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76388; AAC76388; b3363
BAE77927; BAE77927; BAE77927
GeneIDi947870
KEGGiecj:JW3326
eco:b3363
PATRICifig|1411691.4.peg.3367

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55429 Genomic DNA Translation: AAA23451.1
M28363 Genomic DNA Translation: AAA23772.1
U18997 Genomic DNA Translation: AAA58160.1
U00096 Genomic DNA Translation: AAC76388.1
AP009048 Genomic DNA Translation: BAE77927.1
M32354 Genomic DNA Translation: AAA24261.1
PIRiA37964 CSECA
RefSeqiNP_417822.1, NC_000913.3
WP_000477225.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CLHNMR-A25-190[»]
1J2AX-ray1.80A25-190[»]
1V9TX-ray1.70A/B25-190[»]
1VAIX-ray1.80A/B25-190[»]
ProteinModelPortaliP0AFL3
SMRiP0AFL3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261085, 160 interactors
DIPiDIP-48080N
IntActiP0AFL3, 1 interactor
STRINGi316385.ECDH10B_3539

Chemistry databases

DrugBankiDB08168 7-AMINO-4-METHYL-CHROMEN-2-ONE

2D gel databases

SWISS-2DPAGEiP0AFL3

Proteomic databases

EPDiP0AFL3
PaxDbiP0AFL3
PRIDEiP0AFL3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76388; AAC76388; b3363
BAE77927; BAE77927; BAE77927
GeneIDi947870
KEGGiecj:JW3326
eco:b3363
PATRICifig|1411691.4.peg.3367

Organism-specific databases

EchoBASEiEB0750
EcoGeneiEG10757 ppiA

Phylogenomic databases

eggNOGiENOG4108R5K Bacteria
COG0652 LUCA
HOGENOMiHOG000065978
InParanoidiP0AFL3
KOiK03767
OMAiEIFCDEV
PhylomeDBiP0AFL3

Enzyme and pathway databases

BioCyciEcoCyc:EG10757-MONOMER
MetaCyc:EG10757-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0AFL3
PROiPR:P0AFL3

Family and domain databases

Gene3Di2.40.100.10, 1 hit
InterProiView protein in InterPro
IPR029000 Cyclophilin-like_dom_sf
IPR024936 Cyclophilin-type_PPIase
IPR020892 Cyclophilin-type_PPIase_CS
IPR002130 Cyclophilin-type_PPIase_dom
PfamiView protein in Pfam
PF00160 Pro_isomerase, 1 hit
PIRSFiPIRSF001467 Peptidylpro_ismrse, 1 hit
PRINTSiPR00153 CSAPPISMRASE
SUPFAMiSSF50891 SSF50891, 1 hit
PROSITEiView protein in PROSITE
PS00170 CSA_PPIASE_1, 1 hit
PS50072 CSA_PPIASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPIA_ECOLI
AccessioniPrimary (citable) accession number: P0AFL3
Secondary accession number(s): P20752, Q2M729
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 12, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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