UniProtKB - P0AFK9 (POTD_ECOLI)
Protein
Spermidine/putrescine-binding periplasmic protein
Gene
potD
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 36 | Spermidine1 Publication | 1 | |
Binding sitei | 85 | Spermidine1 Publication | 1 | |
Binding sitei | 327 | Spermidine1 Publication | 1 |
GO - Molecular functioni
- putrescine binding Source: EcoCyc
- spermidine binding Source: EcoCyc
GO - Biological processi
- putrescine transport Source: EcoCyc
- spermidine transport Source: EcoCyc
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:POTD-MONOMER MetaCyc:POTD-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.11.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Spermidine/putrescine-binding periplasmic proteinShort name: SPBP |
Gene namesi | Name:potD Ordered Locus Names:b1123, JW1109 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm 1 Publication
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 3 PublicationsAdd BLAST | 23 | |
ChainiPRO_0000031837 | 24 – 348 | Spermidine/putrescine-binding periplasmic proteinAdd BLAST | 325 |
Proteomic databases
jPOSTi | P0AFK9 |
PaxDbi | P0AFK9 |
PRIDEi | P0AFK9 |
2D gel databases
SWISS-2DPAGEi | P0AFK9 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261797, 57 interactors |
ComplexPortali | CPX-4383, Spermidine ABC transporter complex |
IntActi | P0AFK9, 7 interactors |
STRINGi | 511145.b1123 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFK9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFK9 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 168 – 171 | Spermidine-binding1 Publication | 4 |
Sequence similaritiesi
Belongs to the bacterial solute-binding protein PotD/PotF family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0687, Bacteria |
HOGENOMi | CLU_026974_1_3_6 |
InParanoidi | P0AFK9 |
PhylomeDBi | P0AFK9 |
Family and domain databases
InterProi | View protein in InterPro IPR006059, SBP IPR001188, Sperm_putr-bd |
Pfami | View protein in Pfam PF13416, SBP_bac_8, 1 hit |
PIRSFi | PIRSF019574, Periplasmic_polyamine_BP, 1 hit |
PRINTSi | PR00909, SPERMDNBNDNG |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AFK9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKWSRHLLA AGALALGMSA AHADDNNTLY FYNWTEYVPP GLLEQFTKET
60 70 80 90 100
GIKVIYSTYE SNETMYAKLK TYKDGAYDLV VPSTYYVDKM RKEGMIQKID
110 120 130 140 150
KSKLTNFSNL DPDMLNKPFD PNNDYSIPYI WGATAIGVNG DAVDPKSVTS
160 170 180 190 200
WADLWKPEYK GSLLLTDDAR EVFQMALRKL GYSGNTTDPK EIEAAYNELK
210 220 230 240 250
KLMPNVAAFN SDNPANPYME GEVNLGMIWN GSAFVARQAG TPIDVVWPKE
260 270 280 290 300
GGIFWMDSLA IPANAKNKEG ALKLINFLLR PDVAKQVAET IGYPTPNLAA
310 320 330 340
RKLLSPEVAN DKTLYPDAET IKNGEWQNDV GAASSIYEEY YQKLKAGR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64519 Genomic DNA Translation: AAC37041.1 U00096 Genomic DNA Translation: AAC74207.1 AP009048 Genomic DNA Translation: BAA35943.1 |
PIRi | D40840 |
RefSeqi | NP_415641.1, NC_000913.3 WP_000759317.1, NZ_STEB01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC74207; AAC74207; b1123 BAA35943; BAA35943; BAA35943 |
GeneIDi | 58461241 945682 |
KEGGi | ecj:JW1109 eco:b1123 |
PATRICi | fig|1411691.4.peg.1144 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64519 Genomic DNA Translation: AAC37041.1 U00096 Genomic DNA Translation: AAC74207.1 AP009048 Genomic DNA Translation: BAA35943.1 |
PIRi | D40840 |
RefSeqi | NP_415641.1, NC_000913.3 WP_000759317.1, NZ_STEB01000016.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1POT | X-ray | 1.80 | A | 24-348 | [»] | |
1POY | X-ray | 2.50 | 1/2/3/4 | 26-348 | [»] | |
SMRi | P0AFK9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261797, 57 interactors |
ComplexPortali | CPX-4383, Spermidine ABC transporter complex |
IntActi | P0AFK9, 7 interactors |
STRINGi | 511145.b1123 |
Chemistry databases
DrugBanki | DB03566, Spermidine |
Protein family/group databases
TCDBi | 3.A.1.11.1, the atp-binding cassette (abc) superfamily |
2D gel databases
SWISS-2DPAGEi | P0AFK9 |
Proteomic databases
jPOSTi | P0AFK9 |
PaxDbi | P0AFK9 |
PRIDEi | P0AFK9 |
Genome annotation databases
EnsemblBacteriai | AAC74207; AAC74207; b1123 BAA35943; BAA35943; BAA35943 |
GeneIDi | 58461241 945682 |
KEGGi | ecj:JW1109 eco:b1123 |
PATRICi | fig|1411691.4.peg.1144 |
Organism-specific databases
EchoBASEi | EB0745 |
Phylogenomic databases
eggNOGi | COG0687, Bacteria |
HOGENOMi | CLU_026974_1_3_6 |
InParanoidi | P0AFK9 |
PhylomeDBi | P0AFK9 |
Enzyme and pathway databases
BioCyci | EcoCyc:POTD-MONOMER MetaCyc:POTD-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFK9 |
PROi | PR:P0AFK9 |
Family and domain databases
InterProi | View protein in InterPro IPR006059, SBP IPR001188, Sperm_putr-bd |
Pfami | View protein in Pfam PF13416, SBP_bac_8, 1 hit |
PIRSFi | PIRSF019574, Periplasmic_polyamine_BP, 1 hit |
PRINTSi | PR00909, SPERMDNBNDNG |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | POTD_ECOLI | |
Accessioni | P0AFK9Primary (citable) accession number: P0AFK9 Secondary accession number(s): P23861 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families