UniProtKB - P0AFK2 (PNUC_ECOLI)
Protein
Nicotinamide riboside transporter PnuC
Gene
pnuC
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for nicotinamide riboside transport across the inner membrane.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 188 | Nicotinamide ribosideBy similarity | 1 | |
Binding sitei | 192 | Nicotinamide ribosideBy similarity | 1 |
GO - Molecular functioni
- nicotinamide riboside transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- nicotinamide riboside transport Source: EcoCyc
Keywordsi
Biological process | Transport |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:PNUC-MONOMER MetaCyc:PNUC-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Nicotinamide riboside transporter PnuC |
Gene namesi | Name:pnuC Ordered Locus Names:b0751, JW0734 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 2 Publications
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 21 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 22 – 42 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 43 – 48 | PeriplasmicSequence analysis | 6 | |
Transmembranei | 49 – 68 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 69 – 71 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 72 – 89 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 90 – 109 | PeriplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 110 – 127 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 128 – 157 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 158 – 177 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 178 – 183 | PeriplasmicSequence analysis | 6 | |
Transmembranei | 184 – 206 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 207 – 239 | Cytoplasmic1 PublicationAdd BLAST | 33 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058488 | 1 – 239 | Nicotinamide riboside transporter PnuCAdd BLAST | 239 |
Proteomic databases
PaxDbi | P0AFK2 |
PRIDEi | P0AFK2 |
Expressioni
Inductioni
Repressed by NadR.
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259938, 8 interactors |
STRINGi | 511145.b0751 |
Family & Domainsi
Sequence similaritiesi
Belongs to the nicotinamide ribonucleoside (NR) uptake permease (TC 4.B.1) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3201, Bacteria |
HOGENOMi | CLU_076589_1_0_6 |
InParanoidi | P0AFK2 |
PhylomeDBi | P0AFK2 |
Family and domain databases
InterProi | View protein in InterPro IPR006419, NMN_transpt_PnuC |
PANTHERi | PTHR36122, PTHR36122, 1 hit |
Pfami | View protein in Pfam PF04973, NMN_transporter, 1 hit |
TIGRFAMsi | TIGR01528, NMN_trans_PnuC, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFK2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDFFSVQNIL VHIPIGAGGY DLSWIEAVGT IAGLLCIGLA SLEKISNYFF
60 70 80 90 100
GLINVTLFGI IFFQIQLYAS LLLQVFFFAA NIYGWYAWSR QTSQNEAELK
110 120 130 140 150
IRWLPLPKAL SWLAVCVVSI GLMTVFINPV FAFLTRVAVM IMQALGLQVV
160 170 180 190 200
MPELQPDAFP FWDSCMMVLS IVAMILMTRK YVENWLLWVI INVISVVIFA
210 220 230
LQGVYAMSLE YIILTFIALN GSRMWINSAR ERGSRALSH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 6 – 7 | VQ → TH (PubMed:2841129).Curated | 2 | |
Sequence conflicti | 11 | V → I (PubMed:2841129).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73838.1 AP009048 Genomic DNA Translation: BAA35413.1 X12713 Genomic DNA No translation available. |
PIRi | G64810 |
RefSeqi | NP_415272.1, NC_000913.3 WP_000345410.1, NZ_STEB01000028.1 |
Genome annotation databases
EnsemblBacteriai | AAC73838; AAC73838; b0751 BAA35413; BAA35413; BAA35413 |
GeneIDi | 58460880 945350 |
KEGGi | ecj:JW0734 eco:b0751 |
PATRICi | fig|1411691.4.peg.1528 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73838.1 AP009048 Genomic DNA Translation: BAA35413.1 X12713 Genomic DNA No translation available. |
PIRi | G64810 |
RefSeqi | NP_415272.1, NC_000913.3 WP_000345410.1, NZ_STEB01000028.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4259938, 8 interactors |
STRINGi | 511145.b0751 |
Proteomic databases
PaxDbi | P0AFK2 |
PRIDEi | P0AFK2 |
Genome annotation databases
EnsemblBacteriai | AAC73838; AAC73838; b0751 BAA35413; BAA35413; BAA35413 |
GeneIDi | 58460880 945350 |
KEGGi | ecj:JW0734 eco:b0751 |
PATRICi | fig|1411691.4.peg.1528 |
Organism-specific databases
EchoBASEi | EB1651 |
Phylogenomic databases
eggNOGi | COG3201, Bacteria |
HOGENOMi | CLU_076589_1_0_6 |
InParanoidi | P0AFK2 |
PhylomeDBi | P0AFK2 |
Enzyme and pathway databases
BioCyci | EcoCyc:PNUC-MONOMER MetaCyc:PNUC-MONOMER |
Miscellaneous databases
PROi | PR:P0AFK2 |
Family and domain databases
InterProi | View protein in InterPro IPR006419, NMN_transpt_PnuC |
PANTHERi | PTHR36122, PTHR36122, 1 hit |
Pfami | View protein in Pfam PF04973, NMN_transporter, 1 hit |
TIGRFAMsi | TIGR01528, NMN_trans_PnuC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PNUC_ECOLI | |
Accessioni | P0AFK2Primary (citable) accession number: P0AFK2 Secondary accession number(s): P31215, P77227 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 108 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families